Restriction map for
pFN21AB3033
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaBI BsaI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MfeI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 1676 ACGCGT A^C[GA][CT]GT
AgeI 908 ACCGGT A^CCGGT
Alw44I 967 GTGCAC G^TGCAC
AlwNI 898 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1315 CCTCAGG CC^T[ACGT]AGG
ApaLI 967 GTGCAC G^TGCAC
AspI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 12 GGCGCC GGCGC^C
BbvCI 1488 GCTGAGG CCTCAGC(-5/-2)
BlpI 1013 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1013 GCTCAGC GC^T[ACGT]AGC
BsaAI 1063 TACGTG [CT]AC^GT[GA]
BsiEI 977 CGGCCG CG[GA][CT]^CG
BsmI 1494 GAATGC GAATGC(1/-1)
BspHI 1328 TCATGA T^CATGA
BstXI 434 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1315 CCTCAGG CC^T[ACGT]AGG
CelII 1013 GCTCAGC GC^T[ACGT]AGC
DraIII 961 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 977 CGGCCG C^GGCCG
EclXI 977 CGGCCG C^GGCCG
Eco52I 977 CGGCCG C^GGCCG
Eco81I 1315 CCTCAGG CC^T[ACGT]AGG
FseI 57 GGCCGGCC GGCCGG^CC
HaeII 12 GGCGCC [GA]GCGC^[CT]
HincII 1408 GTTGAC GT[CT]^[GA]AC
HindII 1408 GTTGAC GT[CT]^[GA]AC
HinfI 1267 GACTC G^A[ACGT]TC
KasI 12 GGCGCC G^GCGCC
MluI 1676 ACGCGT A^CGCGT
NarI 12 GGCGCC GG^CGCC
PflFI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 908 ACCGGT A^CCGGT
PleI 1267 GACTC GAGTC(4/5)
RcaI 1328 TCATGA T^CATGA
SfoI 12 GGCGCC GGC^GCC
StuI 155 AGGCCT AGG^CCT
Tth111I 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 733 GTCTAC GT^[AC][GT]AC
1369 GTCTAC
AvaI 567 CCCGAG C^[CT]CG[GA]G
1568 CCCGAG
BanI 12 GGCGCC G^G[CT][GA]CC
663 GGCACC
BglI 313 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
712 GCCATGCTGGC
BglII 377 AGATCT A^GATCT
1340 AGATCT
BsaWI 509 TCCGGT [AT]^CCGG[AT]
908 ACCGGT
BsgI 232 GTGCAG GTGCAG(16/14)
1468 GTGCAG
BsmBI 1057 CGTCTC CGTCTC(1/5)
1393 GAGACG
BsoBI 567 CCCGAG C^[CT]CG[GA]G
1568 CCCGAG
BspMI 888 GCAGGT ACCTGC(4/8)
1632 GCAGGT
EcoNI 147 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
744 CCTTCGGGAGG
EcoT14I 258 CCATGG C^C[AT][AT]GG
440 CCATGG
MaeIII 704 GTGAC ^GT[ACGT]AC
817 GTCAC
MseI 254 TTAA T^TAA
629 TTAA
NcoI 258 CCATGG C^CATGG
440 CCATGG
NspI 398 ACATGC [GA]CATG^[CT]
1682 ACATGC
PflMI 787 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
897 CCAGAACCTGG
PvuII 788 CAGCTG CAG^CTG
1402 CAGCTG
StyI 258 CCATGG C^C[AT][AT]GG
440 CCATGG
Tru9I 254 TTAA T^TAA
629 TTAA
Tsp45I 704 GTGAC ^GT[GC]AC
817 GTCAC
Van91I 787 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
897 CCAGAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI ApaI AspHI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I XcmI XhoII