(Circular) (Six-base) MAPSORT of: pFN21AB3033.seq Check: 1013 from: 1 to: 6394 pFN21AB3033 6394 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2884 2884 Size: 6394 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2231 3471 3632 4204 Size: 53 83 186 936 695 1240 161 572 Cuts at: 4204 278 Size: 2468 Fragments arranged by size: 2468 1240 936 695 572 186 161 83 53 AccI GT'mk_AC Cuts at: 1937 2739 3375 3737 1937 Size: 802 636 362 4594 Fragments arranged by size: 4594 802 636 362 Acc65I G'GTAC_C Cuts at: 3715 3715 Size: 6394 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2195 2378 2411 4457 5841 6109 1632 Size: 482 81 183 33 2046 1384 268 1917 Fragments arranged by size: 2046 1917 1384 482 268 183 81 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 3681 5293 6270 1203 Size: 2478 1612 977 1327 Fragments arranged by size: 2478 1612 1327 977 AgeI A'CCGG_T Cuts at: 2913 5225 2913 Size: 2312 4082 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 2841 3488 1166 Size: 1675 647 4072 Fragments arranged by size: 4072 1675 647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6394 AlwNI CAG_nnn'CTG Cuts at: 2908 4137 5709 2908 Size: 1229 1572 3593 Fragments arranged by size: 3593 1572 1229 ApaI G_GGCC'C Cuts at: 2380 2805 3574 3608 2380 Size: 425 769 34 5166 Fragments arranged by size: 5166 769 425 34 ApaLI G'TGCA_C Cuts at: 2972 4451 5607 2972 Size: 1479 1156 3759 Fragments arranged by size: 3759 1479 1156 ApoI r'AATT_y Cuts at: 1493 3703 3929 4081 6190 1493 Size: 2210 226 152 2109 1697 Fragments arranged by size: 2210 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 2572 3573 3719 1870 Size: 73 629 1001 146 4545 Fragments arranged by size: 4545 1001 629 146 73 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2668 3715 5170 618 Size: 325 217 627 230 651 1047 1455 1842 Fragments arranged by size: 1842 1455 1047 651 627 325 230 217 BanII G_rGCy'C Cuts at: 1391 2112 2380 2805 3297 3330 3574 3608 3713 Size: 721 268 425 492 33 244 34 105 Cuts at: 3713 1391 Size: 4072 Fragments arranged by size: 4072 721 492 425 268 244 105 34 33 BbeI G_GCGC'C Cuts at: 2021 2021 Size: 6394 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5423 BbvCI CC'TCA_GC Cuts at: 3494 3494 Size: 6394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4585 4619 1903 Size: 34 50 34 2564 34 3678 Fragments arranged by size: 3678 2564 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4286 5496 4286 Size: 1210 5184 BclI T'GATC_A Cuts at: 1548 1548 Size: 6394 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2324 2723 5005 Size: 107 122 71 631 605 652 399 2282 Cuts at: 5005 136 Size: 1525 Fragments arranged by size: 2282 1525 652 631 605 399 122 107 71 BglII A'GATC_T Cuts at: 2382 3345 6389 2382 Size: 963 3044 2387 Fragments arranged by size: 3044 2387 963 BlpI GC'TnA_GC Cuts at: 3019 3811 3019 Size: 792 5602 Bme1580I G_kGCm'C Cuts at: 1790 2380 2571 2671 2805 2871 2976 3574 3608 Size: 590 191 100 134 66 105 598 34 Cuts at: 3608 4455 5611 1790 Size: 847 1156 2573 Fragments arranged by size: 2573 1156 847 598 590 191 134 105 100 66 34 BmgBI CAC'GTC Cuts at: 1318 2236 2989 1318 Size: 918 753 4723 Fragments arranged by size: 4723 918 753 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 4125 5083 Size: 416 53 83 399 24 1283 1425 958 Cuts at: 5083 442 Size: 1753 Fragments arranged by size: 1753 1425 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2564 2624 2961 3131 3236 3694 5054 1239 Size: 1325 60 337 170 105 458 1360 2579 Fragments arranged by size: 2579 1360 1325 458 337 170 105 60 Bpu10I CC'TnA_GC Cuts at: 2139 3494 2139 Size: 1355 5039 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4519 5384 5682 4519 Size: 865 298 5231 Fragments arranged by size: 5231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5057 882 Size: 617 3558 2219 Fragments arranged by size: 3558 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3070 4185 493 Size: 711 1866 1115 2702 Fragments arranged by size: 2702 1866 1115 711 BsaBI GATnn'nnATC Cuts at: 6388 6388 Size: 6394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2228 3468 3629 Size: 53 83 186 936 485 210 1240 161 Cuts at: 3629 4201 4583 5218 275 Size: 572 382 635 1451 Fragments arranged by size: 1451 1240 936 635 572 485 382 210 186 161 83 53 BsaWI w'CCGG_w Cuts at: 2514 2913 4827 5225 5499 5646 6057 2514 Size: 399 1914 398 274 147 411 2851 Fragments arranged by size: 2851 1914 411 399 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 3212 3242 1178 Size: 30 165 30 669 30 1110 30 4330 Fragments arranged by size: 4330 1110 669 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 2231 3128 3220 3356 1186 Size: 891 154 897 92 136 4224 Fragments arranged by size: 4224 897 891 154 136 92 BseYI C'CCAG_C Cuts at: 3236 5597 6093 3236 Size: 2361 496 3537 Fragments arranged by size: 3537 2361 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 3494 1163 Size: 573 522 1236 4063 Fragments arranged by size: 4063 1236 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2985 3844 4605 5633 1540 Size: 275 186 984 859 761 1028 2301 Fragments arranged by size: 2301 1028 984 859 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2579 2838 2976 3059 3120 3222 4455 4540 5611 Size: 259 138 83 61 102 1233 85 1071 Cuts at: 5611 2579 Size: 3362 Fragments arranged by size: 3362 1233 1071 259 138 102 85 83 61 BsmI GAATG_Cn' Cuts at: 3505 3905 3998 3505 Size: 400 93 5901 Fragments arranged by size: 5901 400 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3068 3392 3068 Size: 324 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2380 2571 2579 2671 2805 2838 Size: 399 322 268 191 8 92 134 33 Cuts at: 2838 2871 2976 3059 3120 3222 3297 3330 3574 Size: 33 105 83 61 102 75 33 244 Cuts at: 3574 3608 3713 4455 4540 5611 1391 Size: 34 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 268 244 191 134 105 105 102 92 85 83 75 61 34 33 33 33 8 BspEI T'CCGG_A Cuts at: 6057 6057 Size: 6394 BspHI T'CATG_A Cuts at: 3333 4283 6013 3333 Size: 950 1730 3714 Fragments arranged by size: 3714 1730 950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2884 3628 3682 3749 844 Size: 2040 744 54 67 3489 Fragments arranged by size: 3489 2040 744 67 54 BsrBI CCG'CTC Cuts at: 1394 1823 2979 3006 3018 4281 1394 Size: 429 1156 27 12 1263 3507 Fragments arranged by size: 3507 1263 1156 429 27 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4889 5063 65 Size: 1183 3641 174 1396 Fragments arranged by size: 3641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2063 2671 2913 3506 5038 5225 Size: 110 204 49 608 242 593 1532 187 Cuts at: 5225 1700 Size: 2869 Fragments arranged by size: 2869 1532 608 593 242 204 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6394 BssSI C'ACGA_G Cuts at: 4454 5466 4454 Size: 1012 5382 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6394 BstXI CCAn_nnnn'nTGG Cuts at: 2446 2446 Size: 6394 BstYI r'GATC_y Cuts at: 1972 2382 2430 3345 3724 4091 4476 4493 5945 Size: 410 48 915 379 367 385 17 1452 Cuts at: 5945 6031 6043 6389 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 915 410 385 379 367 346 86 48 17 12 Bsu36I CC'TnA_GG Cuts at: 3321 3321 Size: 6394 BtgI C'CryG_G Cuts at: 513 1065 2263 2445 3276 513 Size: 552 1198 182 831 3631 Fragments arranged by size: 3631 1198 831 552 182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5655 Fragments arranged by size: 5655 683 56 BtsI GCAGTG_nn' Cuts at: 2640 2712 2869 3180 3453 3910 4704 4724 2640 Size: 72 157 311 273 457 794 20 4310 Fragments arranged by size: 4310 794 457 311 273 157 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6359 DraI TTT'AAA Cuts at: 3698 3698 Size: 6394 DraIII CAC_nnn'GTG Cuts at: 2971 2971 Size: 6394 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5401 809 Size: 507 4085 1802 Fragments arranged by size: 4085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2277 2619 Size: 54 1425 50 275 18 270 177 342 Cuts at: 2619 2724 2982 3037 3086 3252 3841 4730 6359 Size: 105 258 55 49 166 589 889 1629 Cuts at: 6359 8 Size: 43 Fragments arranged by size: 1629 1425 889 589 342 275 270 258 177 166 105 55 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2982 3841 1537 Size: 275 1170 859 4090 Fragments arranged by size: 4090 1170 859 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4324 1353 Size: 2971 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2018 2967 4981 5355 5501 387 Size: 1064 138 429 949 2014 374 146 1280 Fragments arranged by size: 2014 1280 1064 949 429 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2195 2378 2411 2564 2624 2961 Size: 393 482 81 183 33 153 60 337 Cuts at: 2961 3131 3236 3694 4457 5054 5841 6109 1239 Size: 170 105 458 763 597 787 268 1524 Fragments arranged by size: 1524 787 763 597 482 458 393 337 268 183 170 153 105 81 60 33 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2753 2156 Size: 597 5797 EcoO109I rG'GnC_Cy Cuts at: 2170 2353 2802 3009 3159 3316 3570 3604 2170 Size: 183 449 207 150 157 254 34 4960 Fragments arranged by size: 4960 449 254 207 183 157 150 34 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FseI GG_CCGG'CC Cuts at: 2067 2067 Size: 6394 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 2021 5541 1678 Size: 343 3520 2531 Fragments arranged by size: 3520 2531 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3173 3205 5035 5067 5109 Size: 32 135 32 1601 32 1830 32 42 Cuts at: 5109 5141 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 1601 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3415 3738 3984 669 Size: 988 281 1477 323 246 3079 Fragments arranged by size: 3079 1477 988 323 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KasI G'GCGC_C Cuts at: 2017 2017 Size: 6394 KpnI G_GTAC'C Cuts at: 3719 3719 Size: 6394 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 3681 6270 3681 Size: 2589 3805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 3104 5508 5692 1305 Size: 906 893 2404 184 2007 Fragments arranged by size: 2404 2007 906 893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2102 2279 2726 3039 10 Size: 54 1425 343 270 177 447 313 3365 Fragments arranged by size: 3365 1425 447 343 313 270 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2564 2717 2841 3488 4352 4711 4870 Size: 648 1398 153 124 647 864 359 159 Cuts at: 4870 518 Size: 2042 Fragments arranged by size: 2042 1398 864 648 647 359 159 153 124 MspA1I CmG'CkG Cuts at: 1606 2495 2795 3409 3810 4487 5635 5880 1606 Size: 889 300 614 401 677 1148 245 2120 Fragments arranged by size: 2120 1148 889 677 614 401 300 245 NaeI GCC'GGC Cuts at: 2016 2065 3508 2016 Size: 49 1443 4902 Fragments arranged by size: 4902 1443 49 NarI GG'CG_CC Cuts at: 2018 2018 Size: 6394 NcoI C'CATG_G Cuts at: 513 1065 2263 2445 513 Size: 552 1198 182 4462 Fragments arranged by size: 4462 1198 552 182 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NgoMIV G'CCGG_C Cuts at: 2014 2063 3506 2014 Size: 49 1443 4902 Fragments arranged by size: 4902 1443 49 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 2407 3691 3752 5297 1132 Size: 97 1178 1284 61 1545 2229 Fragments arranged by size: 2229 1545 1284 1178 97 61 PasI CC'CwG_GG Cuts at: 1348 3664 1348 Size: 2316 4078 PciI A'CATG_T Cuts at: 5293 5293 Size: 6394 PflMI CCAn_nnn'nTGG Cuts at: 1108 2798 2908 1108 Size: 1690 110 4594 Fragments arranged by size: 4594 1690 110 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4463 4495 5976 6008 4463 Size: 32 1481 32 4849 Fragments arranged by size: 4849 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2170 2353 3009 3159 3316 2170 Size: 183 656 150 157 5248 Fragments arranged by size: 5248 656 183 157 150 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PspOMI G'GGCC_C Cuts at: 2376 2801 3570 3604 2376 Size: 425 769 34 5166 Fragments arranged by size: 5166 769 425 34 PstI C_TGCA'G Cuts at: 830 2494 3148 3175 3408 3746 830 Size: 1664 654 27 233 338 3478 Fragments arranged by size: 3478 1664 654 338 233 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 PvuII CAG'CTG Cuts at: 2795 3409 2795 Size: 614 5780 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SanDI GG'GwC_CC Cuts at: 3009 3009 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SfcI C'TryA_G Cuts at: 826 1045 2490 3144 3171 3404 3742 4877 5558 Size: 219 1445 654 27 233 338 1135 681 Cuts at: 5558 5749 826 Size: 191 1471 Fragments arranged by size: 1471 1445 1135 681 654 338 233 219 191 27 SfoI GGC'GCC Cuts at: 2019 2019 Size: 6394 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3721 1872 Size: 1849 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4498 5399 5661 820 Size: 197 926 2555 901 262 1553 Fragments arranged by size: 2555 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 4318 5 Size: 47 4266 2081 Fragments arranged by size: 4266 2081 47 StuI AGG'CCT Cuts at: 1517 2162 1517 Size: 645 5749 StyI C'CwwG_G Cuts at: 513 1065 2263 2445 3833 513 Size: 552 1198 182 1388 3074 Fragments arranged by size: 3074 1388 1198 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3250 4421 4438 4591 4776 5221 325 Size: 1255 1670 1171 17 153 185 445 1498 Fragments arranged by size: 1670 1498 1255 1171 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3358 3390 4640 Size: 274 80 33 51 1443 1381 32 1250 Cuts at: 4640 6352 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1381 1250 274 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 2259 2291 2692 2724 3212 3244 3600 Size: 32 155 32 401 32 488 32 356 Cuts at: 3600 3632 6354 6386 2072 Size: 32 2722 32 2080 Fragments arranged by size: 2722 2080 488 401 356 155 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2819 1316 Size: 1503 4891 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2200 2446 2533 3560 2200 Size: 246 87 1027 5034 Fragments arranged by size: 5034 1027 246 87 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3719 1870 Size: 1849 4545 XmnI GAAnn'nnTTC Cuts at: 4523 4523 Size: 6394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2229 3469 3630 4202 Size: 53 83 186 936 695 1240 161 572 Cuts at: 4202 276 Size: 2468 Fragments arranged by size: 2468 1240 936 695 572 186 161 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI