Restriction map for
pFN21AE0113
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboII MfeI MflI MluI MluNI
MroI MscI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 66 CGCG CG^CG
AluI 230 AGCT AG^CT
AlwI 84 GATCC GGATC(4/5)
BcnI 249 CCCGG CC^[GC]GG
BsaI 163 GAGACC GGTCTC(1/5)
BseRI 139 GAGGAG GAGGAG(10/8)
BsgI 187 GTGCAG GTGCAG(16/14)
BsmAI 163 GAGAC GTCTC(1/5)
BsmFI 204 GGGAC GGGAC(10/14)
BspMI 104 ACCTGC ACCTGC(4/8)
BsrDI 57 CATTGC GCAATG(2/0)
BsrI 126 CCAGT ACTGG(1/-1)
BstUI 66 CGCG CG^CG
BstXI 26 CCACGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 117 GCGC GCG^C
DpnII 84 GATC ^GATC
DraII 76 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 197 CGGCCA [CT]^GGCC[GA]
EcoO109I 76 GGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 250 CCGG C^CGG
HhaI 117 GCGC GCG^C
HinP1I 117 GCGC G^CGC
HinfI 237 GACTC G^A[ACGT]TC
HpaII 250 CCGG C^CGG
MaeIII 37 GTCAC ^GT[ACGT]AC
MboI 84 GATC ^GATC
MslI 27 CACGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 250 CCGG C^CGG
MvnI 66 CGCG CG^CG
NciI 249 CCCGG CC^[GC]GG
NdeII 84 GATC ^GATC
PleI 237 GACTC GAGTC(4/5)
PstI 112 CTGCAG CTGCA^G
Sau3AI 84 GATC ^GATC
SfcI 112 CTGCAG C^T[GA][CT]AG
SfiI 69 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 37 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 67 GCGG CCGC(-3/-1)
108 GCGG
AvaII 12 GGACC G^G[AT]CC
205 GGACC
BglI 61 GCCATCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
70 GCCCTGGGGGC
BstF5I 51 CATCC GGATG(2/0)
156 GGATG
BstNI 72 CCTGG CC^[AT]GG
87 CCTGG
DsaI 26 CCACGG C^C[GA][CT]GG
258 CCACGG
EcoRII 72 CCTGG ^CC[AT]GG
87 CCTGG
EcoT14I 167 CCAAGG C^C[AT][AT]GG
200 CCAAGG
FokI 51 CATCC GGATG(9/13)
156 GGATG
MvaI 72 CCTGG CC^[AT]GG
87 CCTGG
SfaNI 119 GCATC GCATC(5/9)
146 GCATC
StyI 167 CCAAGG C^C[AT][AT]GG
200 CCAAGG
XcmI 23 CCACCACGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
62 CCATCGCGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
Cfr13I Fnu4HI HaeIII ItaI MnlI
MwoI NlaIV Sau96I ScrFI TseI