Restriction map for
pFN21AE0031
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbrPI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BstBI BstEII
BstPI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FauI FbaI FseI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI MaeII MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1060 GTATAC GT^[AC][GT]AC
AccII 12 CGCG CG^CG
AspHI 1001 GAGCTC G[AT]GC[AT]^C
AviII 1107 TGCGCA TGC^GCA
BbsI 943 GAAGAC GAAGAC(2/6)
BbvCI 1252 GCTGAGG CCTCAGC(-5/-2)
BcgI 646 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 943 GAAGAC GAAGAC(2/6)
BsaBI 1384 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 948 CTGCAC GTGCAG(16/14)
BsiEI 38 CGACCG CG[GA][CT]^CG
BsiHKAI 1001 GAGCTC G[AT]GC[AT]^C
BsmFI 291 GGGAC GGGAC(10/14)
Bsp1407I 833 TGTACA T^GTACA
BsrGI 833 TGTACA T^GTACA
BssSI 727 CACGAG CACGAG(-5/-1)
Bst1107I 1060 GTATAC GTA^TAC
BstUI 12 CGCG CG^CG
BstZ17I 1060 GTATAC GTA^TAC
CfoI 1108 GCGC GCG^C
DraII 1352 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 277 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1356 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1352 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 335 CCATGG C^C[AT][AT]GG
EcoT22I 107 ATGCAT ATGCA^T
FspI 1107 TGCGCA TGC^GCA
HhaI 1108 GCGC GCG^C
HinP1I 1108 GCGC G^CGC
HphI 1381 GGTGA GGTGA(8/7)
KspI 11 CCGCGG CCGC^GG
MamI 1384 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 12 CGCG CG^CG
NcoI 335 CCATGG C^CATGG
NdeI 824 CATATG CA^TATG
NheI 184 GCTAGC G^CTAGC
NsiI 107 ATGCAT ATGCA^T
NspI 343 GCATGT [GA]CATG^[CT]
PleI 1332 GACTC GAGTC(4/5)
PpuMI 1352 AGGACCT [GA]G^G[AT]CC[CT]
PstI 747 CTGCAG CTGCA^G
PvuII 1111 CAGCTG CAG^CTG
SacI 1001 GAGCTC GAGCT^C
SacII 11 CCGCGG CCGC^GG
SapI 998 GAAGAGC GCTCTTC(1/4)
SfcI 747 CTGCAG C^T[GA][CT]AG
SspBI 833 TGTACA T^GTACA
StyI 335 CCATGG C^C[AT][AT]GG
Tsp45I 599 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 508 GAATTT [GA]^AATT[CT]
1461 GAATTT
ApoI 508 GAATTT [GA]^AATT[CT]
1461 GAATTT
Asp700I 611 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1275 GAAGAAGTTC
BanII 362 GAGCCC G[GA]GC[CT]^C
1001 GAGCTC
BcnI 234 CCGGG CC^[GC]GG
288 CCGGG
BsmAI 85 GAGAC GTCTC(1/5)
1046 GAGAC
BsrDI 741 CATTGC GCAATG(2/0)
559 GCAATG
BsrI 372 ACTGG ACTGG(1/-1)
1247 CCAGT
BstAPI 109 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
740 GCATTGCCTGC
DsaI 11 CCGCGG C^C[GA][CT]GG
335 CCATGG
MaeIII 599 GTGAC ^GT[ACGT]AC
1010 GTTAC
MseI 800 TTAA T^TAA
908 TTAA
MspA1I 11 CCGCGG C[AC]G^C[GT]G
1111 CAGCTG
NciI 234 CCGGG CC^[GC]GG
288 CCGGG
SmlI 541 CTCAAG C^T[CT][GA]AG
993 CTTGAG
StuI 734 AGGCCT AGG^CCT
1298 AGGCCT
TaqI 56 TCGA T^CGA
201 TCGA
TfiI 616 GATTC G^A[AT]TC
1152 GATTC
Tru9I 800 TTAA T^TAA
908 TTAA
TthHB8I 56 TCGA T^CGA
201 TCGA
XmnI 611 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1275 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I
TspRI