(Circular) (Six-base) MAPSORT of: pFN21AE0031.seq Check: 1257 from: 1 to: 6187 pFN21AE0031 6187 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3066 3530 1937 Size: 1129 464 4594 Fragments arranged by size: 4594 1129 464 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3424 4250 5634 5902 1632 Size: 1792 826 1384 268 1917 Fragments arranged by size: 1917 1792 1384 826 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6187 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3269 3301 3269 Size: 32 6155 AlwNI CAG_nnn'CTG Cuts at: 3930 5502 3930 Size: 1572 4615 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2513 3466 3496 3722 3874 5983 1493 Size: 1020 953 30 226 152 2109 1697 Fragments arranged by size: 2109 1697 1020 953 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3508 4963 618 Size: 325 217 627 1721 1455 1842 Fragments arranged by size: 1842 1721 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2371 3010 3506 1391 Size: 980 639 496 4072 Fragments arranged by size: 4072 980 639 496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2955 928 Size: 971 1056 4160 Fragments arranged by size: 4160 1056 971 BbvCI CC'TCA_GC Cuts at: 3258 3258 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2640 2674 4378 4412 1903 Size: 34 703 34 1704 34 3678 Fragments arranged by size: 3678 1704 703 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BfrBI ATG'CAT Cuts at: 2114 2114 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4798 136 Size: 107 122 71 631 605 337 2789 1525 Fragments arranged by size: 2789 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2509 4248 5404 1790 Size: 719 1739 1156 2573 Fragments arranged by size: 2573 1739 1156 719 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2193 1056 Size: 1137 5050 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3024 3024 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4847 1239 Size: 3608 2579 Bpu10I CC'TnA_GC Cuts at: 2394 2924 3258 2394 Size: 530 334 5323 Fragments arranged by size: 5323 530 334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2531 3019 4312 5177 5475 2531 Size: 488 1293 865 298 3243 Fragments arranged by size: 3243 1293 865 488 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 3393 6181 3393 Size: 2788 3399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 1178 Size: 30 165 30 1866 30 4066 Fragments arranged by size: 4066 1866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3287 3290 3383 1186 Size: 2101 3 93 3990 Fragments arranged by size: 3990 2101 93 3 BseYI C'CCAG_C Cuts at: 2569 5390 5886 2569 Size: 2821 496 2870 Fragments arranged by size: 2870 2821 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2938 1163 Size: 573 1202 4412 Fragments arranged by size: 4412 1202 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 3637 4398 5426 1540 Size: 275 186 45 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 3010 4248 4333 5404 3010 Size: 1238 85 1071 3793 Fragments arranged by size: 3793 1238 1071 85 BsmI GAATG_Cn' Cuts at: 2341 2422 3181 3416 3698 3791 2341 Size: 81 759 235 282 93 4737 Fragments arranged by size: 4737 759 282 235 93 81 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2371 2509 3010 3506 4248 4333 5404 Size: 399 581 138 501 496 742 85 1071 Cuts at: 5404 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 581 501 496 399 138 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3542 844 Size: 2698 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 2745 4682 4856 65 Size: 1183 1323 174 1937 174 1396 Fragments arranged by size: 1937 1396 1323 1183 174 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4831 5018 1700 Size: 110 3021 187 2869 Fragments arranged by size: 3021 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2838 96 Size: 2742 3445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 2732 4247 5259 2732 Size: 1515 1012 3660 Fragments arranged by size: 3660 1515 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2120 2751 794 Size: 1326 631 4230 Fragments arranged by size: 4230 1326 631 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 3517 3884 4269 4286 5738 5824 5836 6182 Size: 1545 367 385 17 1452 86 12 346 Cuts at: 6182 1972 Size: 1977 Fragments arranged by size: 1977 1545 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3067 3067 Size: 6187 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2340 513 Size: 552 939 12 324 4360 Fragments arranged by size: 4360 939 552 324 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 2516 487 Size: 683 56 814 476 4158 Fragments arranged by size: 4158 814 683 476 56 BtsI GCAGTG_nn' Cuts at: 2970 3703 4497 4517 2970 Size: 733 794 20 4640 Fragments arranged by size: 4640 794 733 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2288 5194 809 Size: 507 972 2906 1802 Fragments arranged by size: 2906 1802 972 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152 Size: 54 1425 50 275 18 1804 889 1629 Cuts at: 6152 8 Size: 43 Fragments arranged by size: 1804 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2998 3125 4117 1353 Size: 719 926 127 992 3423 Fragments arranged by size: 3423 992 926 719 127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3008 3008 Size: 6187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3424 4250 4847 5634 5902 1239 Size: 393 1792 826 597 787 268 1524 Fragments arranged by size: 1792 1524 826 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3365 3365 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 3358 3358 Size: 6187 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3436 3436 Size: 6187 FspI TGC'GCA Cuts at: 3114 4693 3114 Size: 1579 4608 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3214 3246 4828 4860 4902 Size: 32 135 32 1642 32 1582 32 42 Cuts at: 4902 4934 1373 Size: 32 2626 Fragments arranged by size: 2626 1642 1582 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3531 3777 669 Size: 988 281 1593 246 3079 Fragments arranged by size: 3079 1593 988 281 246 HindIII A'AGCT_T Cuts at: 748 2142 2921 2969 3201 748 Size: 1394 779 48 232 3734 Fragments arranged by size: 3734 1394 779 232 48 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 3277 5301 5485 1305 Size: 951 1021 2024 184 2007 Fragments arranged by size: 2024 2007 1021 951 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2219 2388 3380 4145 4504 4663 Size: 648 1005 48 169 992 765 359 159 Cuts at: 4663 518 Size: 2042 Fragments arranged by size: 2042 1005 992 765 648 359 169 159 48 MspA1I CmG'CkG Cuts at: 1606 2018 3118 3603 4280 5428 5673 1606 Size: 412 1100 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 1100 677 485 412 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2340 513 Size: 552 939 336 4360 Fragments arranged by size: 4360 939 552 336 NdeI CA'TA_TG Cuts at: 387 2830 387 Size: 2443 3744 NheI G'CTAG_C Cuts at: 1052 2189 1052 Size: 1137 5050 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NsiI A_TGCA'T Cuts at: 2116 2116 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 2352 3545 5090 1132 Size: 97 1123 1193 1545 2229 Fragments arranged by size: 2229 1545 1193 1123 97 PasI CC'CwG_GG Cuts at: 1348 2851 1348 Size: 1503 4684 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3269 3301 4256 4288 5769 5801 3269 Size: 32 955 32 1481 32 3655 Fragments arranged by size: 3655 1481 955 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3358 3358 Size: 6187 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6187 PsiI TTA'TAA Cuts at: 2148 3757 2148 Size: 1609 4578 PstI C_TGCA'G Cuts at: 830 2756 3539 830 Size: 1926 783 3478 Fragments arranged by size: 3478 1926 783 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 3118 3118 Size: 6187 SacI G_AGCT'C Cuts at: 3010 3010 Size: 6187 SacII CC_GC'GG Cuts at: 2019 2019 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SapI GCTCTTCn'nnn_ Cuts at: 2998 2998 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2752 3535 4670 5351 5542 826 Size: 219 1707 783 1135 681 191 1471 Fragments arranged by size: 1707 1471 1135 783 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 2998 4291 5192 5454 820 Size: 197 926 603 452 1293 901 262 1553 Fragments arranged by size: 1553 1293 926 901 603 452 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 2741 3305 1517 Size: 1224 564 4399 Fragments arranged by size: 4399 1224 564 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3626 513 Size: 552 939 336 1286 3074 Fragments arranged by size: 3074 1286 939 552 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 3374 4214 4231 4384 4569 5014 Size: 1255 480 1314 840 17 153 185 445 Cuts at: 5014 325 Size: 1498 Fragments arranged by size: 1498 1314 1255 840 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2838 4433 6145 Size: 274 80 33 51 1443 861 1595 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 1712 1595 1443 861 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 2620 3284 4316 2620 Size: 664 1032 4491 Fragments arranged by size: 4491 1032 664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI