(Circular) (Six-base) MAPSORT of: pFN21AE0016.seq Check: 1618 from: 1 to: 6928 pFN21AE0016 6928 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2379 4738 278 Size: 53 83 186 936 843 2359 2468 Fragments arranged by size: 2468 2359 936 843 186 83 53 AccI GT'mk_AC Cuts at: 1937 2224 4271 1937 Size: 287 2047 4594 Fragments arranged by size: 4594 2047 287 Acc65I G'GTAC_C Cuts at: 4249 4249 Size: 6928 AclI AA'CG_TT Cuts at: 1563 5055 5428 1563 Size: 3492 373 3063 Fragments arranged by size: 3492 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2800 3150 3936 4038 4991 6375 6643 1632 Size: 1168 350 786 102 953 1384 268 1917 Fragments arranged by size: 1917 1384 1168 953 786 350 268 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6731 AflIII A'CryG_T Cuts at: 1203 5827 6804 1203 Size: 4624 977 1327 Fragments arranged by size: 4624 1327 977 AgeI A'CCGG_T Cuts at: 5759 5759 Size: 6928 AhdI GACnn_n'nnGTC Cuts at: 5657 5657 Size: 6928 AleI CACnn'nnGTG Cuts at: 1166 2903 1166 Size: 1737 5191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6928 AlwNI CAG_nnn'CTG Cuts at: 3060 4671 6243 3060 Size: 1611 1572 3745 Fragments arranged by size: 3745 1611 1572 ApaI G_GGCC'C Cuts at: 2888 2888 Size: 6928 ApaLI G'TGCA_C Cuts at: 2486 4985 6141 2486 Size: 2499 1156 3273 Fragments arranged by size: 3273 2499 1156 ApoI r'AATT_y Cuts at: 1493 2726 4237 4463 4615 6724 1493 Size: 1233 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 1233 226 152 AseI AT'TA_AT Cuts at: 160 1032 3944 5482 160 Size: 872 2912 1538 1606 Fragments arranged by size: 2912 1606 1538 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 AvaI C'yCGr_G Cuts at: 1870 1943 2299 3773 4253 1870 Size: 73 356 1474 480 4545 Fragments arranged by size: 4545 1474 480 356 73 BamHI G'GATC_C Cuts at: 3150 4258 4625 3150 Size: 1108 367 5453 Fragments arranged by size: 5453 1108 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4249 5704 618 Size: 325 217 627 2462 1455 1842 Fragments arranged by size: 2462 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2888 3298 4247 1391 Size: 1497 410 949 4072 Fragments arranged by size: 4072 1497 949 410 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 3138 928 Size: 971 472 767 4718 Fragments arranged by size: 4718 971 767 472 BbvCI CC'TCA_GC Cuts at: 3609 3609 Size: 6928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2483 2517 5119 5153 1903 Size: 34 546 34 2602 34 3678 Fragments arranged by size: 3678 2602 546 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4820 6030 4820 Size: 1210 5718 BclI T'GATC_A Cuts at: 1548 3955 1548 Size: 2407 4521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2444 3102 5539 Size: 107 122 71 631 605 772 658 2437 Cuts at: 5539 136 Size: 1525 Fragments arranged by size: 2437 1525 772 658 631 605 122 107 71 BglII A'GATC_T Cuts at: 3790 6923 3790 Size: 3133 3795 BlpI GC'TnA_GC Cuts at: 4345 4345 Size: 6928 Bme1580I G_kGCm'C Cuts at: 1790 2490 2539 2882 2888 4989 6145 1790 Size: 700 49 343 6 2101 1156 2573 Fragments arranged by size: 2573 2101 1156 700 343 49 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6928 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4659 5617 442 Size: 416 53 83 399 24 3242 958 1753 Fragments arranged by size: 3242 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6928 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2859 2887 3082 5588 1239 Size: 1620 28 195 2506 2579 Fragments arranged by size: 2579 2506 1620 195 28 Bpu10I CC'TnA_GC Cuts at: 2795 3299 3450 3609 2795 Size: 504 151 159 6114 Fragments arranged by size: 6114 504 159 151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4016 5053 5918 6216 4016 Size: 1037 865 298 4728 Fragments arranged by size: 4728 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5591 882 Size: 617 4092 2219 Fragments arranged by size: 4092 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2329 2860 3805 4719 493 Size: 711 1125 531 945 914 2702 Fragments arranged by size: 2702 1125 945 914 711 531 BsaBI GATnn'nnATC Cuts at: 2028 3795 6922 2028 Size: 1767 3127 2034 Fragments arranged by size: 3127 2034 1767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2376 2447 3778 4735 Size: 53 83 186 936 843 71 1331 957 Cuts at: 4735 5117 5752 275 Size: 382 635 1451 Fragments arranged by size: 1451 1331 957 936 843 635 382 186 83 71 53 BsaWI w'CCGG_w Cuts at: 3723 5361 5759 6033 6180 6591 3723 Size: 1638 398 274 147 411 4060 Fragments arranged by size: 4060 1638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2115 2145 2903 2933 3605 Size: 30 165 30 712 30 758 30 672 Cuts at: 3605 3635 1178 Size: 30 4471 Fragments arranged by size: 4471 758 712 672 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2801 3626 3875 1186 Size: 1615 825 249 4239 Fragments arranged by size: 4239 1615 825 249 BseYI C'CCAG_C Cuts at: 2686 2846 3254 3343 3988 6131 6627 2686 Size: 160 408 89 645 2143 496 2987 Fragments arranged by size: 2987 2143 645 496 408 160 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 2414 2541 3701 1163 Size: 573 594 84 127 1160 4390 Fragments arranged by size: 4390 1160 594 573 127 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2518 4378 5139 6167 1540 Size: 275 186 517 1860 761 1028 2301 Fragments arranged by size: 2301 1860 1028 761 517 275 186 BsiHKAI G_wGCw'C Cuts at: 2490 3128 3427 3855 4989 5074 6145 2490 Size: 638 299 428 1134 85 1071 3273 Fragments arranged by size: 3273 1134 1071 638 428 299 85 BsmI GAATG_Cn' Cuts at: 2605 2983 3823 4439 4532 2605 Size: 378 840 616 93 5001 Fragments arranged by size: 5001 840 616 378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 2539 2882 2888 3128 3298 3427 Size: 399 700 49 343 6 240 170 129 Cuts at: 3427 3855 4247 4989 5074 6145 1391 Size: 428 392 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 700 428 399 392 343 240 170 129 85 49 6 BspEI T'CCGG_A Cuts at: 6591 6591 Size: 6928 BspHI T'CATG_A Cuts at: 2206 4817 6547 2206 Size: 2611 1730 2587 Fragments arranged by size: 2611 2587 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4283 844 Size: 3439 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4815 1394 Size: 429 2992 3507 Fragments arranged by size: 3507 2992 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3327 5423 5597 65 Size: 1183 2079 2096 174 1396 Fragments arranged by size: 2096 2079 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2256 2890 5572 5759 1700 Size: 110 446 634 2682 187 2869 Fragments arranged by size: 2869 2682 634 446 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6928 BssSI C'ACGA_G Cuts at: 4988 6000 4988 Size: 1012 5916 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6928 BstBI TT'CG_AA Cuts at: 5732 5732 Size: 6928 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6928 BstYI r'GATC_y Cuts at: 1972 3150 3790 4258 4625 5010 5027 6479 6565 Size: 1178 640 468 367 385 17 1452 86 Cuts at: 6565 6577 6923 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1178 640 468 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2316 4069 4155 513 Size: 552 939 312 1753 86 3286 Fragments arranged by size: 3286 1753 939 552 312 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6189 Fragments arranged by size: 6189 683 56 BtsI GCAGTG_nn' Cuts at: 2655 4444 5238 5258 2655 Size: 1789 794 20 4325 Fragments arranged by size: 4325 1789 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6928 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6893 DraI TTT'AAA Cuts at: 2694 4232 2694 Size: 1538 5390 DraIII CAC_nnn'GTG Cuts at: 2317 2496 2317 Size: 179 6749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5935 809 Size: 507 4619 1802 Fragments arranged by size: 4619 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2457 2515 Size: 54 1425 50 275 18 177 450 58 Cuts at: 2515 2652 3136 4116 4375 5264 6893 8 Size: 137 484 980 259 889 1629 43 Fragments arranged by size: 1629 1425 980 889 484 450 275 259 177 137 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2515 4375 1537 Size: 275 703 1860 4090 Fragments arranged by size: 4090 1860 703 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3922 4858 1353 Size: 1466 1103 936 3423 Fragments arranged by size: 3423 1466 1103 936 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3412 5515 5889 6035 387 Size: 1064 138 1823 2103 374 146 1280 Fragments arranged by size: 2103 1823 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2800 2859 2887 3082 3150 3936 4038 Size: 393 1168 59 28 195 68 786 102 Cuts at: 4038 4991 5588 6375 6643 1239 Size: 953 597 787 268 1524 Fragments arranged by size: 1524 1168 953 787 786 597 393 268 195 102 68 59 28 EcoO109I rG'GnC_Cy Cuts at: 2167 2194 2806 2167 Size: 27 612 6289 Fragments arranged by size: 6289 612 27 EcoRI G'AATT_C Cuts at: 4237 4615 4237 Size: 378 6550 FspI TGC'GCA Cuts at: 3383 5434 3383 Size: 2051 4877 HaeII r_GCGC'y Cuts at: 1678 6075 1678 Size: 4397 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2366 2398 3142 Size: 32 135 32 543 32 219 32 744 Cuts at: 3142 3174 3605 3637 5569 5601 5643 5675 1373 Size: 32 431 32 1932 32 42 32 2626 Fragments arranged by size: 2626 1932 744 543 431 219 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2158 2335 4272 4518 669 Size: 988 281 220 177 1937 246 3079 Fragments arranged by size: 3079 1937 988 281 246 220 177 HindIII A'AGCT_T Cuts at: 748 4020 748 Size: 3272 3656 HpaI GTT'AAC Cuts at: 2158 4518 2158 Size: 2360 4568 KpnI G_GTAC'C Cuts at: 4253 4253 Size: 6928 MfeI C'AATT_G Cuts at: 4527 4527 Size: 6928 MluI A'CGCG_T Cuts at: 6804 6804 Size: 6928 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1995 3904 6042 6226 1305 Size: 690 1909 2138 184 2007 Fragments arranged by size: 2138 2007 1909 690 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2654 4118 10 Size: 54 1425 343 822 1464 2820 Fragments arranged by size: 2820 1464 1425 822 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2714 2903 2939 3807 4886 5245 Size: 648 843 705 189 36 868 1079 359 Cuts at: 5245 5404 518 Size: 159 2042 Fragments arranged by size: 2042 1079 868 843 705 648 359 189 159 36 MspA1I CmG'CkG Cuts at: 1606 3335 3387 3503 3656 4344 5021 6169 6414 Size: 1729 52 116 153 688 677 1148 245 Cuts at: 6414 1606 Size: 2120 Fragments arranged by size: 2120 1729 1148 688 677 245 153 116 52 NaeI GCC'GGC Cuts at: 2892 2892 Size: 6928 NcoI C'CATG_G Cuts at: 513 1065 2004 4069 513 Size: 552 939 2065 3372 Fragments arranged by size: 3372 2065 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6928 NgoMIV G'CCGG_C Cuts at: 2890 2890 Size: 6928 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6928 NotI GC'GGCC_GC Cuts at: 4375 4375 Size: 6928 NspI r_CATG'y Cuts at: 1132 1229 2794 2938 4286 5831 1132 Size: 97 1565 144 1348 1545 2229 Fragments arranged by size: 2229 1565 1545 1348 144 97 PasI CC'CwG_GG Cuts at: 1348 2188 2442 1348 Size: 840 254 5834 Fragments arranged by size: 5834 840 254 PciI A'CATG_T Cuts at: 5827 5827 Size: 6928 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 2619 1108 Size: 1209 302 5417 Fragments arranged by size: 5417 1209 302 PmeI GTTT'AAAC Cuts at: 4232 4232 Size: 6928 PmlI CAC'GTG Cuts at: 2329 3805 2329 Size: 1476 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4997 5029 6510 6542 4997 Size: 32 1481 32 5383 Fragments arranged by size: 5383 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 2194 2167 Size: 27 6901 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6928 PsiI TTA'TAA Cuts at: 4498 4498 Size: 6928 PspOMI G'GGCC_C Cuts at: 2884 2884 Size: 6928 PstI C_TGCA'G Cuts at: 830 2406 4280 830 Size: 1576 1874 3478 Fragments arranged by size: 3478 1874 1576 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6928 PvuII CAG'CTG Cuts at: 3503 3503 Size: 6928 SalI G'TCGA_C Cuts at: 1936 4270 1936 Size: 2334 4594 SbfI CC_TGCA'GG Cuts at: 4280 4280 Size: 6928 ScaI AGT'ACT Cuts at: 5176 6888 5176 Size: 1712 5216 SfcI C'TryA_G Cuts at: 826 1045 2402 3599 4276 5411 6092 6283 826 Size: 219 1357 1197 677 1135 681 191 1471 Fragments arranged by size: 1471 1357 1197 1135 681 677 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6928 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6928 SmaI CCC'GGG Cuts at: 1872 4255 1872 Size: 2383 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4031 5032 5933 6195 820 Size: 197 926 2088 1001 901 262 1553 Fragments arranged by size: 2088 1553 1001 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4719 493 Size: 4226 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6928 SphI G_CATG'C Cuts at: 1132 2794 2938 4286 1132 Size: 1662 144 1348 3774 Fragments arranged by size: 3774 1662 1348 144 SspI AAT'ATT Cuts at: 5 52 4852 5 Size: 47 4800 2081 Fragments arranged by size: 4800 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6928 StyI C'CwwG_G Cuts at: 513 1065 2004 2560 4069 4367 513 Size: 552 939 556 1509 298 3074 Fragments arranged by size: 3074 1509 939 556 552 298 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 4955 4972 5125 5310 5755 325 Size: 1255 1031 2344 17 153 185 445 1498 Fragments arranged by size: 2344 1498 1255 1031 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 5174 6886 Size: 274 80 33 51 1443 429 2768 1712 Cuts at: 6886 96 Size: 138 Fragments arranged by size: 2768 1712 1443 429 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6888 6920 6888 Size: 32 6896 Tth111I GACn'n_nGTC Cuts at: 1316 2129 1316 Size: 813 6115 XbaI T'CTAG_A Cuts at: 4108 4264 4108 Size: 156 6772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6928 XmaI C'CCGG_G Cuts at: 1870 4253 1870 Size: 2383 4545 XmnI GAAnn'nnTTC Cuts at: 5057 5057 Size: 6928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2377 4736 276 Size: 53 83 186 936 843 2359 2468 Fragments arranged by size: 2468 2359 936 843 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI