(Circular) (Six-base) MAPSORT of: pFN21AE0062.seq Check: 1272 from: 1 to: 6607 pFN21AE0062 6607 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 3002 3950 1937 Size: 697 368 948 4594 Fragments arranged by size: 4594 948 697 368 Acc65I G'GTAC_C Cuts at: 3928 3928 Size: 6607 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 3759 4670 6054 6322 1632 Size: 1056 1071 911 1384 268 1917 Fragments arranged by size: 1917 1384 1071 1056 911 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 2259 2302 2472 3175 5506 6483 1203 Size: 1056 43 170 703 2331 977 1327 Fragments arranged by size: 2331 1327 1056 977 703 170 43 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6607 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3795 3827 3795 Size: 32 6575 AlwNI CAG_nnn'CTG Cuts at: 2742 2860 4350 5922 2742 Size: 118 1490 1572 3427 Fragments arranged by size: 3427 1572 1490 118 ApaLI G'TGCA_C Cuts at: 4664 5820 4664 Size: 1156 5451 ApoI r'AATT_y Cuts at: 1493 3230 3853 3916 4142 4294 6403 1493 Size: 1737 623 63 226 152 2109 1697 Fragments arranged by size: 2109 1737 1697 623 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 2096 2615 3932 1870 Size: 73 153 519 1317 4545 Fragments arranged by size: 4545 1317 519 153 73 BamHI G'GATC_C Cuts at: 2042 3208 3937 4304 2042 Size: 1166 729 367 4345 Fragments arranged by size: 4345 1166 729 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2214 3928 5383 618 Size: 325 217 627 427 1714 1455 1842 Fragments arranged by size: 1842 1714 1455 627 427 325 217 BanII G_rGCy'C Cuts at: 1391 2628 3542 3926 1391 Size: 1237 914 384 4072 Fragments arranged by size: 4072 1237 914 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2365 928 Size: 971 466 5170 Fragments arranged by size: 5170 971 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3093 3127 4798 4832 1903 Size: 34 1156 34 1671 34 3678 Fragments arranged by size: 3678 1671 1156 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2487 4499 5709 2487 Size: 2012 1210 3385 Fragments arranged by size: 3385 2012 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6602 6602 Size: 6607 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 4668 5824 1790 Size: 2878 1156 2573 Fragments arranged by size: 2878 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2631 4338 5296 Size: 416 53 83 399 24 1214 1707 958 Cuts at: 5296 442 Size: 1753 Fragments arranged by size: 1753 1707 1214 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 5267 1239 Size: 966 3062 2579 Fragments arranged by size: 3062 2579 966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4732 5597 5895 4732 Size: 865 298 5444 Fragments arranged by size: 5444 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5270 882 Size: 617 3771 2219 Fragments arranged by size: 3771 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4398 493 Size: 711 3194 2702 Fragments arranged by size: 3194 2702 711 BsaBI GATnn'nnATC Cuts at: 2433 6601 2433 Size: 4168 2439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2821 4414 4796 5431 Size: 53 83 186 936 1288 1593 382 635 Cuts at: 5431 275 Size: 1451 Fragments arranged by size: 1593 1451 1288 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 5040 5438 5712 5859 6270 2205 Size: 2835 398 274 147 411 2542 Fragments arranged by size: 2835 2542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3795 3825 1178 Size: 30 165 30 2392 30 3960 Fragments arranged by size: 3960 2392 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2151 2202 1186 Size: 965 51 5591 Fragments arranged by size: 5591 965 51 BseYI C'CCAG_C Cuts at: 3892 5810 6306 3892 Size: 1918 496 4193 Fragments arranged by size: 4193 1918 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6034 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4057 4818 5846 1540 Size: 275 186 2056 761 1028 2301 Fragments arranged by size: 2301 2056 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 3259 3542 4668 4753 5824 2811 Size: 448 283 1126 85 1071 3594 Fragments arranged by size: 3594 1126 1071 448 283 85 BsmI GAATG_Cn' Cuts at: 4118 4211 4118 Size: 93 6514 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2628 2811 3259 3542 3926 4668 4753 Size: 399 838 183 448 283 384 742 85 Cuts at: 4753 5824 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 838 742 448 399 384 283 183 85 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 2524 3217 4496 6226 2524 Size: 693 1279 1730 2905 Fragments arranged by size: 2905 1730 1279 693 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3962 844 Size: 3118 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4494 1394 Size: 429 2671 3507 Fragments arranged by size: 3507 2671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5102 5276 65 Size: 1183 3854 174 1396 Fragments arranged by size: 3854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5251 5438 1700 Size: 110 3441 187 2869 Fragments arranged by size: 3441 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 4667 5679 4667 Size: 1012 5595 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6607 BstBI TT'CG_AA Cuts at: 3634 5411 3634 Size: 1777 4830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2042 2456 3208 3937 4304 4689 4706 6158 Size: 70 414 752 729 367 385 17 1452 Cuts at: 6158 6244 6256 6602 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 752 729 414 385 367 346 86 70 17 12 BstZ17I GTA'TAC Cuts at: 2635 2635 Size: 6607 BtgI C'CryG_G Cuts at: 513 1065 3090 513 Size: 552 2025 4030 Fragments arranged by size: 4030 2025 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5868 Fragments arranged by size: 5868 683 56 BtsI GCAGTG_nn' Cuts at: 2761 2809 4123 4917 4937 2761 Size: 48 1314 794 20 4431 Fragments arranged by size: 4431 1314 794 48 20 ClaI AT'CG_AT Cuts at: 1554 3324 1554 Size: 1770 4837 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5614 809 Size: 507 4298 1802 Fragments arranged by size: 4298 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4054 4943 6572 Size: 54 1425 50 275 18 2224 889 1629 Cuts at: 6572 8 Size: 43 Fragments arranged by size: 2224 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4054 1537 Size: 275 2242 4090 Fragments arranged by size: 4090 2242 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 3778 4537 1353 Size: 731 1694 759 3423 Fragments arranged by size: 3423 1694 759 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5194 5568 5714 387 Size: 1064 138 3605 374 146 1280 Fragments arranged by size: 3605 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3540 3540 Size: 6607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2688 3759 4670 5267 6054 6322 Size: 393 573 483 1071 911 597 787 268 Cuts at: 6322 1239 Size: 1524 Fragments arranged by size: 1524 1071 911 787 597 573 483 393 268 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 EcoRV GAT'ATC Cuts at: 2920 3641 2920 Size: 721 5886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3326 3326 Size: 6607 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 5754 1678 Size: 4076 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3165 3197 5248 5280 5322 Size: 32 135 32 1593 32 2051 32 42 Cuts at: 5322 5354 1373 Size: 32 2626 Fragments arranged by size: 2626 2051 1593 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3951 4197 669 Size: 988 281 2013 246 3079 Fragments arranged by size: 3079 2013 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KpnI G_GTAC'C Cuts at: 3932 3932 Size: 6607 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5721 5905 1305 Size: 4416 184 2007 Fragments arranged by size: 4416 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4785 Fragments arranged by size: 4785 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 3366 4565 4924 5083 518 Size: 648 1357 843 1199 359 159 2042 Fragments arranged by size: 2042 1357 1199 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2684 2860 3155 3241 4023 4700 5848 6093 Size: 1078 176 295 86 782 677 1148 245 Cuts at: 6093 1606 Size: 2120 Fragments arranged by size: 2120 1148 1078 782 677 295 245 176 86 NcoI C'CATG_G Cuts at: 513 1065 3090 513 Size: 552 2025 4030 Fragments arranged by size: 4030 2025 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 2263 2306 2407 2446 2476 2984 3179 Size: 97 1034 43 101 39 30 508 195 Cuts at: 3179 3350 3965 5510 1132 Size: 171 615 1545 2229 Fragments arranged by size: 2229 1545 1034 615 508 195 171 101 97 43 39 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 2259 2302 2472 3175 5506 2259 Size: 43 170 703 2331 3360 Fragments arranged by size: 3360 2331 703 170 43 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 3749 1108 Size: 1752 889 3966 Fragments arranged by size: 3966 1752 889 PfoI T'CCnGG_A Cuts at: 3204 3204 Size: 6607 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3795 3827 4676 4708 6189 6221 3795 Size: 32 849 32 1481 32 4181 Fragments arranged by size: 4181 1481 849 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PspXI vC'TCGA_Gb Cuts at: 2096 2096 Size: 6607 PstI C_TGCA'G Cuts at: 830 2683 3959 830 Size: 1853 1276 3478 Fragments arranged by size: 3478 1853 1276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 2684 2860 3155 3241 2684 Size: 176 295 86 6050 Fragments arranged by size: 6050 295 176 86 SacI G_AGCT'C Cuts at: 3542 3542 Size: 6607 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 2574 4855 6567 2574 Size: 2281 1712 2614 Fragments arranged by size: 2614 2281 1712 SfcI C'TryA_G Cuts at: 826 1045 2679 3955 5090 5771 5962 826 Size: 219 1634 1276 1135 681 191 1471 Fragments arranged by size: 1634 1471 1276 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2615 4711 5612 5874 820 Size: 197 926 153 519 2096 901 262 1553 Fragments arranged by size: 2096 1553 926 901 519 262 197 153 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 3725 152 Size: 3573 3034 SphI G_CATG'C Cuts at: 1132 2446 3350 3965 1132 Size: 1314 904 615 3774 Fragments arranged by size: 3774 1314 904 615 SspI AAT'ATT Cuts at: 5 52 2561 3193 4531 5 Size: 47 2509 632 1338 2081 Fragments arranged by size: 2509 2081 1338 632 47 StuI AGG'CCT Cuts at: 1517 3578 1517 Size: 2061 4546 StyI C'CwwG_G Cuts at: 513 1065 2871 3090 3573 3645 3903 4046 513 Size: 552 1806 219 483 72 258 143 3074 Fragments arranged by size: 3074 1806 552 483 258 219 143 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4634 4651 4804 4989 5434 325 Size: 1255 3054 17 153 185 445 1498 Fragments arranged by size: 3054 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2572 4853 6565 Size: 274 80 33 51 1443 595 2281 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 2281 1712 1443 595 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6567 6599 6567 Size: 32 6575 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XhoI C'TCGA_G Cuts at: 1943 2096 2615 1943 Size: 153 519 5935 Fragments arranged by size: 5935 519 153 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 3273 4736 3273 Size: 1463 5144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI