(Circular) (Six-base) MAPSORT of: pFN21AB8440.seq Check: 7960 from: 1 to: 5968 pFN21AB8440 5968 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2717 2717 Size: 5968 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 2211 3289 2211 Size: 1078 4890 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4031 5415 5683 1632 Size: 2399 1384 268 1917 Fragments arranged by size: 2399 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 2075 2219 2602 4867 5844 1203 Size: 872 144 383 2265 977 1327 Fragments arranged by size: 2265 1327 977 872 383 144 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 2220 1166 Size: 1054 4914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 3711 5283 3711 Size: 1572 4396 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2239 3277 3503 3655 5764 1493 Size: 746 1038 226 152 2109 1697 Fragments arranged by size: 2109 1697 1038 746 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 3293 1870 Size: 73 1350 4545 Fragments arranged by size: 4545 1350 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 2356 3289 4744 618 Size: 325 217 627 424 145 933 1455 1842 Fragments arranged by size: 1842 1455 933 627 424 325 217 145 BanII G_rGCy'C Cuts at: 1391 3246 3287 1391 Size: 1855 41 4072 Fragments arranged by size: 4072 1855 41 BbeI G_GCGC'C Cuts at: 2360 2360 Size: 5968 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3203 928 Size: 971 1304 3693 Fragments arranged by size: 3693 1304 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2697 3860 5070 2697 Size: 1163 1210 3595 Fragments arranged by size: 3595 1210 1163 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2187 5963 2187 Size: 3776 2192 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 2806 4029 5185 1790 Size: 1016 1223 1156 2573 Fragments arranged by size: 2573 1223 1156 1016 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2635 3699 4657 Size: 416 53 83 399 24 1218 1064 958 Cuts at: 4657 442 Size: 1753 Fragments arranged by size: 1753 1218 1064 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2320 3057 4628 1239 Size: 929 152 737 1571 2579 Fragments arranged by size: 2579 1571 929 737 152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4093 4958 5256 4093 Size: 865 298 4805 Fragments arranged by size: 4805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3227 4631 882 Size: 617 1728 1404 2219 Fragments arranged by size: 2219 1728 1404 617 BsaAI yAC'GTr Cuts at: 493 1204 2076 2196 2603 3759 493 Size: 711 872 120 407 1156 2702 Fragments arranged by size: 2702 1156 872 711 407 120 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2357 3775 4157 4792 Size: 53 83 186 936 824 1418 382 635 Cuts at: 4792 275 Size: 1451 Fragments arranged by size: 1451 1418 936 824 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4401 4799 5073 5220 5631 4401 Size: 398 274 147 411 4738 Fragments arranged by size: 4738 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5968 BseYI C'CCAG_C Cuts at: 2535 2547 2787 3030 5171 5667 2535 Size: 12 240 243 2141 496 2836 Fragments arranged by size: 2836 2141 496 243 240 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2133 1163 Size: 573 397 4998 Fragments arranged by size: 4998 573 397 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2930 3418 4179 5207 1540 Size: 275 186 929 488 761 1028 2301 Fragments arranged by size: 2301 1028 929 761 488 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 3246 4029 4114 5185 2256 Size: 990 783 85 1071 3039 Fragments arranged by size: 3039 1071 990 783 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2256 2806 3246 3287 4029 4114 5185 Size: 399 466 550 440 41 742 85 1071 Cuts at: 5185 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 466 440 399 85 41 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 2395 3857 5587 2395 Size: 1462 1730 2776 Fragments arranged by size: 2776 1730 1462 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2717 3323 844 Size: 1873 606 3489 Fragments arranged by size: 3489 1873 606 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2430 4463 4637 65 Size: 1183 1182 2033 174 1396 Fragments arranged by size: 2033 1396 1183 1182 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2543 3022 4612 4799 1700 Size: 110 733 479 1590 187 2869 Fragments arranged by size: 2869 1590 733 479 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 2438 4028 5040 2438 Size: 1590 1012 3366 Fragments arranged by size: 3366 1590 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5968 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2222 2035 Size: 187 5781 BstYI r'GATC_y Cuts at: 1972 2187 2608 3298 3665 4050 4067 5519 5605 Size: 215 421 690 367 385 17 1452 86 Cuts at: 5605 5617 5963 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 690 421 385 367 346 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 513 Size: 552 939 48 4429 Fragments arranged by size: 4429 939 552 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2256 2266 3484 4278 4298 2052 Size: 204 10 1218 794 20 3722 Fragments arranged by size: 3722 1218 794 204 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DraIII CAC_nnn'GTG Cuts at: 2120 2120 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 2832 2901 Size: 54 1425 50 275 18 711 291 69 Cuts at: 2901 3415 4304 5933 8 Size: 514 889 1629 43 Fragments arranged by size: 1629 1425 889 711 514 291 275 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 3898 1353 Size: 1203 1342 3423 Fragments arranged by size: 3423 1342 1203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3244 3244 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2320 3057 4031 4628 5415 5683 Size: 393 536 152 737 974 597 787 268 Cuts at: 5683 1239 Size: 1524 Fragments arranged by size: 1524 974 787 737 597 536 393 268 152 EcoO109I rG'GnC_Cy Cuts at: 2160 2160 Size: 5968 EcoRI G'AATT_C Cuts at: 2239 3277 3655 2239 Size: 1038 378 4552 Fragments arranged by size: 4552 1038 378 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 2360 2473 2763 2818 3166 5115 1678 Size: 682 113 290 55 348 1949 2531 Fragments arranged by size: 2531 1949 682 348 290 113 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2238 2270 2437 2469 4609 Size: 32 135 32 666 32 167 32 2140 Cuts at: 4609 4641 4683 4715 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2140 666 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3558 669 Size: 988 281 1374 246 3079 Fragments arranged by size: 3079 1374 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KasI G'GCGC_C Cuts at: 2356 2356 Size: 5968 KpnI G_GTAC'C Cuts at: 2215 3293 2215 Size: 1078 4890 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3013 3231 5082 5266 1305 Size: 1708 218 1851 184 2007 Fragments arranged by size: 2007 1851 1708 218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4146 Fragments arranged by size: 4146 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2220 3926 4285 4444 518 Size: 648 1054 1706 359 159 2042 Fragments arranged by size: 2042 1706 1054 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2724 3384 4061 5209 5454 1606 Size: 1118 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 677 660 245 NaeI GCC'GGC Cuts at: 2545 2545 Size: 5968 NarI GG'CG_CC Cuts at: 2357 2357 Size: 5968 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4477 Fragments arranged by size: 4477 939 552 NdeI CA'TA_TG Cuts at: 387 2332 387 Size: 1945 4023 NgoMIV G'CCGG_C Cuts at: 2543 2543 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2223 3326 4871 1132 Size: 97 994 1103 1545 2229 Fragments arranged by size: 2229 1545 1103 994 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5968 PciI A'CATG_T Cuts at: 2219 4867 2219 Size: 2648 3320 PflMI CCAn_nnn'nTGG Cuts at: 1108 2120 1108 Size: 1012 4956 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PmlI CAC'GTG Cuts at: 2076 2603 2076 Size: 527 5441 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PstI C_TGCA'G Cuts at: 830 3320 830 Size: 2490 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 SacI G_AGCT'C Cuts at: 3246 3246 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 2691 3167 3316 4451 5132 5323 826 Size: 219 1646 476 149 1135 681 191 1471 Fragments arranged by size: 1646 1471 1135 681 476 219 191 149 SfoI GGC'GCC Cuts at: 2358 2358 Size: 5968 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 3295 1872 Size: 1423 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4072 4973 5235 820 Size: 197 926 2129 901 262 1553 Fragments arranged by size: 2129 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 2511 1517 Size: 994 4974 StyI C'CwwG_G Cuts at: 513 1065 2004 2978 3142 3407 513 Size: 552 939 974 164 265 3074 Fragments arranged by size: 3074 974 939 552 265 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 2527 3995 4012 4165 4350 4795 Size: 1255 659 288 1468 17 153 185 445 Cuts at: 4795 325 Size: 1498 Fragments arranged by size: 1498 1468 1255 659 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4214 5926 96 Size: 274 80 33 51 1443 2237 1712 138 Fragments arranged by size: 2237 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3121 3153 5928 5960 3121 Size: 32 2775 32 3129 Fragments arranged by size: 3129 2775 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2648 2648 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 3293 1870 Size: 1423 4545 XmnI GAAnn'nnTTC Cuts at: 4097 4097 Size: 5968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI