Restriction map for
pFN21AE0096
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# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspI AviII AvrII BbrPI
BcgI BfrI BglI BglII BlnI
BsaAI BsiEI BsiWI BsmBI BspDI
BsrFI BssHII BstXI Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI FspI HpaI KspI
MfeI MluI MunI NaeI NgoMIV
NotI PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfiI
SgrAI SmaI SnaBI SpeI SphI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 180 CGCG CG^CG
AccIII 27 TCCGGA T^CCGGA
AcyI 21 GGCGCC G[GA]^CG[CT]C
AhdI 4504 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4504 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3302 TGGCCA TGG^CCA
BanII 3272 GAGCTC G[GA]GC[CT]^C
BbeI 21 GGCGCC GGCGC^C
BbvCI 4381 CCTCAGC CCTCAGC(-5/-2)
BclI 4227 TGATCA T^GATCA
BcnI 620 CCGGG CC^[GC]GG
BsaBI 4981 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsaI 145 GGTCTC GGTCTC(1/5)
BsaWI 27 TCCGGA [AT]^CCGG[AT]
BseAI 27 TCCGGA T^CCGGA
BspEI 27 TCCGGA T^CCGGA
BssSI 1670 CTCGTG CACGAG(-5/-1)
Bst1107I 4289 GTATAC GTA^TAC
BstBI 3435 TTCGAA TT^CGAA
BstEII 3513 GGTAACC G^GT[ACGT]ACC
BstPI 3513 GGTAACC G^GT[ACGT]ACC
BstUI 180 CGCG CG^CG
BstZ17I 4289 GTATAC GTA^TAC
DraII 2208 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 446 CCATGG C^C[GA][CT]GG
EaeI 3302 TGGCCA [CT]^GGCC[GA]
Eam1105I 4504 GACGTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2008 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2208 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3513 GGTAACC G^GT[ACGT]ACC
EcoT22I 3862 ATGCAT ATGCA^T
FbaI 4227 TGATCA T^GATCA
HaeII 21 GGCGCC [GA]GCGC^[CT]
HgaI 426 GCGTC GACGC(5/10)
Hin1I 21 GGCGCC G[GA]^CG[CT]C
HincII 2453 GTCAAC GT[CT]^[GA]AC
HindII 2453 GTCAAC GT[CT]^[GA]AC
KasI 21 GGCGCC G^GCGCC
MamI 4981 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3302 TGGCCA TGG^CCA
MroI 27 TCCGGA T^CCGGA
MscI 3302 TGGCCA TGG^CCA
MvnI 180 CGCG CG^CG
NarI 21 GGCGCC GG^CGCC
NciI 620 CCGGG CC^[GC]GG
NcoI 446 CCATGG C^CATGG
NheI 5397 GCTAGC G^CTAGC
NruI 179 TCGCGA TCG^CGA
NsiI 3862 ATGCAT ATGCA^T
NspV 3435 TTCGAA TT^CGAA
PflMI 4840 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2208 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3901 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3272 GAGCTC GAGCT^C
SapI 2857 GCTCTTC GCTCTTC(1/4)
SbfI 1906 CCTGCAGG CCTGCA^GG
SfoI 21 GGCGCC GGC^GCC
SfuI 3435 TTCGAA TT^CGAA
Sse8387I 1906 CCTGCAGG CCTGCA^GG
StuI 990 AGGCCT AGG^CCT
Van91I 4840 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 336 GGTACC G^GTACC
2334 GGTACC
AflIII 1331 ACATGT A^C[GA][CT]GT
3768 ACATGT
AocI 3283 CCTCAGG CC^T[ACGT]AGG
3407 CCTCAGG
Asp700I 239 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
881 GAAATCTTTC
Asp718I 336 GGTACC G^GTACC
2334 GGTACC
AvaI 1255 CTCGAG C^[CT]CG[GA]G
1622 CTCGAG
BamHI 643 GGATCC G^GATCC
4849 GGATCC
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
3349 GCTCAGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
3349 GCTCAGC
BsmI 3135 GAATGC GAATGC(1/-1)
4531 GAATGC
BsoBI 1255 CTCGAG C^[CT]CG[GA]G
1622 CTCGAG
BspHI 768 TCATGA T^CATGA
3774 TCATGA
BspLU11I 1331 ACATGT A^CATGT
3768 ACATGT
BsrBI 34 GAGCGG CCGCTC(-3/-3)
3609 GAGCGG
Bsu36I 3283 CCTCAGG CC^T[ACGT]AGG
3407 CCTCAGG
CelII 1018 GCTGAGC GC^T[ACGT]AGC
3349 GCTCAGC
Eco81I 3283 CCTCAGG CC^T[ACGT]AGG
3407 CCTCAGG
EcoT14I 446 CCATGG C^C[AT][AT]GG
3492 CCAAGG
FauI 4767 CCCGC CCCGC(4/6)
852 GCGGG
HapII 28 CCGG C^CGG
620 CCGG
HindIII 3226 AAGCTT A^AGCTT
5417 AAGCTT
HpaII 28 CCGG C^CGG
620 CCGG
KpnI 336 GGTACC GGTAC^C
2334 GGTACC
MspI 28 CCGG C^CGG
620 CCGG
NdeI 2398 CATATG CA^TATG
4748 CATATG
PaeR7I 1255 CTCGAG C^TCGAG
1622 CTCGAG
RcaI 768 TCATGA T^CATGA
3774 TCATGA
SexAI 3216 ACCAGGT A^CC[AT]GGT
3509 ACCAGGT
StyI 446 CCATGG C^C[AT][AT]GG
3492 CCAAGG
XcmI 3492 CCAAGGGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5402 CCATTAGTAACATGG
XhoI 1255 CTCGAG C^TCGAG
1622 CTCGAG
XmnI 239 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
881 GAAATCTTTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraIII EarI
EcoRI EcoRII EcoRV Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII