Restriction map for
pFN21AE0096
(Linear) (Six-base) MAPSORT of: KIEE0096.seq Check: 4167 from: 1 to: 5505
KIEE0096.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1914 3558 5505
Size: 1914 1644 1947
Fragments arranged by size:
1947 1914 1644
AccI GT'mk_AC
Cuts at: 0 3909 4290 4513 5505
Size: 3909 381 223 992
Fragments arranged by size:
3909 992 381 223
Acc65I G'GTAC_C
Cuts at: 0 336 2334 5505
Size: 336 1998 3171
Fragments arranged by size:
3171 1998 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 2336 2579 5505
Size: 844 1492 243 2926
Fragments arranged by size:
2926 1492 844 243
AflIII A'CryG_T
Cuts at: 0 1331 3768 5505
Size: 1331 2437 1737
Fragments arranged by size:
2437 1737 1331
AhdI GACnn_n'nnGTC
Cuts at: 0 4509 5505
Size: 4509 996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1541 2105 4232 5505
Size: 1541 564 2127 1273
Fragments arranged by size:
2127 1541 1273 564
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1707 1739 5505
Size: 1707 32 3766
Fragments arranged by size:
3766 1707 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2101 3189 4963 5505
Size: 2101 1088 1774 542
Fragments arranged by size:
2101 1774 1088 542
ApoI r'AATT_y
Cuts at: 0 1127 2048 2160 2271 2604 3932 4241 4368
Size: 1127 921 112 111 333 1328 309 127
Cuts at: 4368 4586 5505
Size: 218 919
Fragments arranged by size:
1328 1127 921 919 333 309 218 127
112 111
AvaI C'yCGr_G
Cuts at: 0 1255 1622 5505
Size: 1255 367 3883
Fragments arranged by size:
3883 1255 367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2318 2351 5505
Size: 2318 33 3154
Fragments arranged by size:
3154 2318 33
BamHI G'GATC_C
Cuts at: 0 643 4849 5505
Size: 643 4206 656
Fragments arranged by size:
4206 656 643
BanI G'GyrC_C
Cuts at: 0 21 336 2334 5505
Size: 21 315 1998 3171
Fragments arranged by size:
3171 1998 315 21
BanII G_rGCy'C
Cuts at: 0 3276 5505
Size: 3276 2229
BbeI G_GCGC'C
Cuts at: 0 25 5505
Size: 25 5480
BbsI GAAGACnn'nnnn_
Cuts at: 0 688 928 5129 5505
Size: 688 240 4201 376
Fragments arranged by size:
4201 688 376 240
BbvCI CC'TCA_GC
Cuts at: 0 4382 5505
Size: 4382 1123
BciVI GTATCCnnnnn_n'
Cuts at: 0 3837 4488 4554 5505
Size: 3837 651 66 951
Fragments arranged by size:
3837 951 651 66
BclI T'GATC_A
Cuts at: 0 4227 5505
Size: 4227 1278
BfrBI ATG'CAT
Cuts at: 0 3864 5505
Size: 3864 1641
BlpI GC'TnA_GC
Cuts at: 0 1019 3350 5505
Size: 1019 2331 2155
Fragments arranged by size:
2331 2155 1019
Bme1580I G_kGCm'C
Cuts at: 0 3431 5167 5505
Size: 3431 1736 338
Fragments arranged by size:
3431 1736 338
BmgBI CAC'GTC
Cuts at: 0 567 5505
Size: 567 4938
BmrI ACTGGGnnnn_n'
Cuts at: 0 5204 5505
Size: 5204 301
BmtI G_CTAG'C
Cuts at: 0 5401 5505
Size: 5401 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 2078 3329 3575 4883 5505
Size: 1520 558 1251 246 1308 622
Fragments arranged by size:
1520 1308 1251 622 558 246
Bpu10I CC'TnA_GC
Cuts at: 0 4382 5505
Size: 4382 1123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2649 3644 3888 5505
Size: 2649 995 244 1617
Fragments arranged by size:
2649 1617 995 244
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 5505
Size: 151 5354
BsaBI GATnn'nnATC
Cuts at: 0 4985 5505
Size: 4985 520
BsaHI Gr'CG_yC
Cuts at: 0 22 5505
Size: 22 5483
BsaWI w'CCGG_w
Cuts at: 0 27 5505
Size: 27 5478
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 200 230 2171 2201 3684 3714 3790 3820
Size: 200 30 1941 30 1483 30 76 30
Cuts at: 3820 5505
Size: 1685
Fragments arranged by size:
1941 1685 1483 200 76 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 910 2219 3754 3833 5247 5505
Size: 910 1309 1535 79 1414 258
Fragments arranged by size:
1535 1414 1309 910 258 79
BseYI C'CCAG_C
Cuts at: 0 825 1494 2931 3237 3477 5505
Size: 825 669 1437 306 240 2028
Fragments arranged by size:
2028 1437 825 669 306 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 3352 4828 5201 5505
Size: 941 2411 1476 373 304
Fragments arranged by size:
2411 1476 941 373 304
BsiHKAI G_wGCw'C
Cuts at: 0 3276 3958 5187 5505
Size: 3276 682 1229 318
Fragments arranged by size:
3276 1229 682 318
BsmI GAATG_Cn'
Cuts at: 0 3141 4537 5505
Size: 3141 1396 968
Fragments arranged by size:
3141 1396 968
Bsp1286I G_dGCh'C
Cuts at: 0 3276 3431 3958 5167 5187 5505
Size: 3276 155 527 1209 20 318
Fragments arranged by size:
3276 1209 527 318 155 20
BspEI T'CCGG_A
Cuts at: 0 27 5505
Size: 27 5478
BspHI T'CATG_A
Cuts at: 0 768 3774 5505
Size: 768 3006 1731
Fragments arranged by size:
3006 1731 768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1914 3063 3310 3558 5505
Size: 1914 1149 247 248 1947
Fragments arranged by size:
1947 1914 1149 248 247
BsrBI CCG'CTC
Cuts at: 0 36 3611 5505
Size: 36 3575 1894
Fragments arranged by size:
3575 1894 36
BsrDI GCAATG_nn'
Cuts at: 0 909 1770 2281 2684 3404 3584 3843 5505
Size: 909 861 511 403 720 180 259 1662
Fragments arranged by size:
1662 909 861 720 511 403 259 180
BsrGI T'GTAC_A
Cuts at: 0 2010 4752 4800 5505
Size: 2010 2742 48 705
Fragments arranged by size:
2742 2010 705 48
BssSI C'ACGA_G
Cuts at: 0 1670 5505
Size: 1670 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 0 570 1387 3670 5505
Size: 570 817 2283 1835
Fragments arranged by size:
2283 1835 817 570
BstBI TT'CG_AA
Cuts at: 0 3436 5505
Size: 3436 2069
BstEII G'GTnAC_C
Cuts at: 0 3513 5505
Size: 3513 1992
BstYI r'GATC_y
Cuts at: 0 135 585 643 2345 4680 4849 4866 5505
Size: 135 450 58 1702 2335 169 17 639
Fragments arranged by size:
2335 1702 639 450 169 135 58 17
BstZ17I GTA'TAC
Cuts at: 0 4291 5505
Size: 4291 1214
Bsu36I CC'TnA_GG
Cuts at: 0 3284 3408 5505
Size: 3284 124 2097
Fragments arranged by size:
3284 2097 124
BtgI C'CryG_G
Cuts at: 0 446 5505
Size: 446 5059
BtsI GCAGTG_nn'
Cuts at: 0 875 5002 5505
Size: 875 4127 503
Fragments arranged by size:
4127 875 503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 497 532 642 677 5505
Size: 497 35 110 35 4828
Fragments arranged by size:
4828 497 110 35 35
DraI TTT'AAA
Cuts at: 0 102 266 1109 3881 5505
Size: 102 164 843 2772 1624
Fragments arranged by size:
2772 1624 843 164 102
DraIII CAC_nnn'GTG
Cuts at: 0 411 2331 3220 5505
Size: 411 1920 889 2285
Fragments arranged by size:
2285 1920 889 411
EaeI y'GGCC_r
Cuts at: 0 3302 5505
Size: 3302 2203
EarI CTCTTCn'nnn_
Cuts at: 0 215 1735 2864 5505
Size: 215 1520 1129 2641
Fragments arranged by size:
2641 1520 1129 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 188 5505
Size: 188 5317
EcoICRI GAG'CTC
Cuts at: 0 3274 5505
Size: 3274 2231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1520 2078 2336 2579 3329 3575 4883
Size: 844 676 558 258 243 750 246 1308
Cuts at: 4883 5505
Size: 622
Fragments arranged by size:
1308 844 750 676 622 558 258 246
243
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2012 5505
Size: 2012 3493
EcoO109I rG'GnC_Cy
Cuts at: 0 2209 5505
Size: 2209 3296
EcoRI G'AATT_C
Cuts at: 0 2048 2160 2271 4368 4586 5505
Size: 2048 112 111 2097 218 919
Fragments arranged by size:
2097 2048 919 218 112 111
EcoRV GAT'ATC
Cuts at: 0 4044 4169 4200 5505
Size: 4044 125 31 1305
Fragments arranged by size:
4044 1305 125 31
HaeII r_GCGC'y
Cuts at: 0 25 5505
Size: 25 5480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1197 1229 1818 1850 3790 3822 4430 4453
Size: 1197 32 589 32 1940 32 608 23
Cuts at: 4453 4462 4485 4495 4527 5505
Size: 9 23 10 32 978
Fragments arranged by size:
1940 1197 978 608 589 32 32 32
32 23 23 10 9
HincII GTy'rAC
Cuts at: 0 2455 5505
Size: 2455 3050
HindIII A'AGCT_T
Cuts at: 0 3226 5417 5505
Size: 3226 2191 88
Fragments arranged by size:
3226 2191 88
KasI G'GCGC_C
Cuts at: 0 21 5505
Size: 21 5484
KpnI G_GTAC'C
Cuts at: 0 340 2338 5505
Size: 340 1998 3167
Fragments arranged by size:
3167 1998 340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 2181 3609 5328 5505
Size: 395 1786 1428 1719 177
Fragments arranged by size:
1786 1719 1428 395 177
MscI TGG'CCA
Cuts at: 0 3304 5505
Size: 3304 2201
MslI CAynn'nnrTG
Cuts at: 0 1279 1330 1336 1781 1825 3773 4780 5210
Size: 1279 51 6 445 44 1948 1007 430
Cuts at: 5210 5505
Size: 295
Fragments arranged by size:
1948 1279 1007 445 430 295 51 44
6
MspA1I CmG'CkG
Cuts at: 0 852 1018 2512 2866 4798 5034 5314 5505
Size: 852 166 1494 354 1932 236 280 191
Fragments arranged by size:
1932 1494 852 354 280 236 191 166
NarI GG'CG_CC
Cuts at: 0 22 5505
Size: 22 5483
NcoI C'CATG_G
Cuts at: 0 446 5505
Size: 446 5059
NdeI CA'TA_TG
Cuts at: 0 2399 4749 5505
Size: 2399 2350 756
Fragments arranged by size:
2399 2350 756
NheI G'CTAG_C
Cuts at: 0 5397 5505
Size: 5397 108
NruI TCG'CGA
Cuts at: 0 181 5505
Size: 181 5324
NsiI A_TGCA'T
Cuts at: 0 3866 5505
Size: 3866 1639
NspI r_CATG'y
Cuts at: 0 1272 1335 1525 2432 3772 3868 4038 5505
Size: 1272 63 190 907 1340 96 170 1467
Fragments arranged by size:
1467 1340 1272 907 190 170 96 63
PciI A'CATG_T
Cuts at: 0 1331 3768 5505
Size: 1331 2437 1737
Fragments arranged by size:
2437 1737 1331
PflMI CCAn_nnn'nTGG
Cuts at: 0 4846 5505
Size: 4846 659
PfoI T'CCnGG_A
Cuts at: 0 56 1536 5505
Size: 56 1480 3969
Fragments arranged by size:
3969 1480 56
PpuMI rG'GwC_Cy
Cuts at: 0 2209 5505
Size: 2209 3296
PshAI GACnn'nnGTC
Cuts at: 0 3905 5505
Size: 3905 1600
PsiI TTA'TAA
Cuts at: 0 473 1703 5505
Size: 473 1230 3802
Fragments arranged by size:
3802 1230 473
PspXI vC'TCGA_Gb
Cuts at: 0 1255 5505
Size: 1255 4250
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2003 2035 5505
Size: 2003 32 3470
Fragments arranged by size:
3470 2003 32
PstI C_TGCA'G
Cuts at: 0 1911 4648 5504 5505
Size: 1911 2737 856 1
Fragments arranged by size:
2737 1911 856 1
PvuII CAG'CTG
Cuts at: 0 1018 2512 2866 4798 5314 5505
Size: 1018 1494 354 1932 516 191
Fragments arranged by size:
1932 1494 1018 516 354 191
SacI G_AGCT'C
Cuts at: 0 3276 5505
Size: 3276 2229
SapI GCTCTTCn'nnn_
Cuts at: 0 2864 5505
Size: 2864 2641
SbfI CC_TGCA'GG
Cuts at: 0 1911 5505
Size: 1911 3594
SexAI A'CCwGG_T
Cuts at: 0 3216 3509 5505
Size: 3216 293 1996
Fragments arranged by size:
3216 1996 293
SfcI C'TryA_G
Cuts at: 0 1197 1454 1907 2147 2438 2618 2723 4644
Size: 1197 257 453 240 291 180 105 1921
Cuts at: 4644 4691 5156 5500 5505
Size: 47 465 344 5
Fragments arranged by size:
1921 1197 465 453 344 291 257 240
180 105 47 5
SfoI GGC'GCC
Cuts at: 0 23 5505
Size: 23 5482
SmlI C'TyrA_G
Cuts at: 0 1255 1622 2664 3659 3903 5505
Size: 1255 367 1042 995 244 1602
Fragments arranged by size:
1602 1255 1042 995 367 244
SspI AAT'ATT
Cuts at: 0 1011 2580 3449 5324 5334 5344 5505
Size: 1011 1569 869 1875 10 10 161
Fragments arranged by size:
1875 1569 1011 869 161 10 10
StuI AGG'CCT
Cuts at: 0 992 5505
Size: 992 4513
StyI C'CwwG_G
Cuts at: 0 446 3492 5505
Size: 446 3046 2013
Fragments arranged by size:
3046 2013 446
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 1424 4258 4499 5505
Size: 109 1315 2834 241 1006
Fragments arranged by size:
2834 1315 1006 241 109
TatI w'GTAC_w
Cuts at: 0 1829 2010 2242 2412 2431 2598 3081 3105
Size: 1829 181 232 170 19 167 483 24
Cuts at: 3105 3417 3639 3688 3893 4752 4800 5505
Size: 312 222 49 205 859 48 705
Fragments arranged by size:
1829 859 705 483 312 232 222 205
181 170 167 49 48 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 5505
Size: 1399 32 4074
Fragments arranged by size:
4074 1399 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3499 5409 5505
Size: 3499 1910 96
Fragments arranged by size:
3499 1910 96
XhoI C'TCGA_G
Cuts at: 0 1255 1622 5505
Size: 1255 367 3883
Fragments arranged by size:
3883 1255 367
XmnI GAAnn'nnTTC
Cuts at: 0 243 885 5505
Size: 243 642 4620
Fragments arranged by size:
4620 642 243
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NruI NsiI NspI PciI PflMI PfoI PpuMI
PshAI PsiI PspXI PsrI PstI PvuII SacI SapI
SbfI SexAI SfcI SfoI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaI ApaLI
AscI AseI AsiSI AvrII BcgI BglI BglII BplI
BsaAI BsiEI BsiWI BsmBI BsrFI BssHII BstXI BtgZI
ClaI DrdI EagI FalI FseI FspI FspAI HpaI
MfeI MluI NaeI NgoMIV NotI PacI PasI PmeI
PmlI PpiI PspOMI PvuI RsrII SacII SalI SanDI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XmaI ZraI