Restriction map for
pFN21AE0093
##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AocI AscI AseI AsnI AviII
AvrII BalI BamHI BbrPI BciVI
BfrI BlnI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BsrDI BstBI
Bsu36I ClaI DraI DrdI EarI
Eco81I EcoRV EcoT22I FseI FspI
HpaI Ksp632I MamI MfeI MluI
MluNI MroI MscI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SapI ScaI
SfuI SnaBI SpeI SspI SwaI
TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3215 GGTACC G^GTACC
AcsI 2324 GAATTC [GA]^AATT[CT]
AhdI 2758 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3221 AGCGCT AGC^GCT
ApoI 2324 GAATTC [GA]^AATT[CT]
Asp700I 2634 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3215 GGTACC G^GTACC
AspEI 2758 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1129 GAAGAC GAAGAC(2/6)
BbvCI 574 GCTGAGG CCTCAGC(-5/-2)
BclI 536 TGATCA T^GATCA
BfaI 1345 CTAG C^TAG
BglII 2252 AGATCT A^GATCT
BpuAI 1129 GAAGAC GAAGAC(2/6)
BsaAI 2946 TACGTG [CT]AC^GT[GA]
BsmI 1446 GAATGC GAATGC(1/-1)
BspHI 779 TCATGA T^CATGA
BspLU11I 1672 ACATGT A^CATGT
Bst1107I 790 GTATAC GTA^TAC
BstEII 2655 GGTGACC G^GT[ACGT]ACC
BstPI 2655 GGTGACC G^GT[ACGT]ACC
BstZ17I 790 GTATAC GTA^TAC
CpoI 2830 CGGACCG CG^G[AT]CCG
Eam1105I 2758 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3221 AGCGCT AGC^GCT
EcoO65I 2655 GGTGACC G^GT[ACGT]ACC
EcoRI 2324 GAATTC G^AATTC
FbaI 536 TGATCA T^GATCA
HindIII 847 AAGCTT A^AGCTT
KpnI 3215 GGTACC GGTAC^C
KspI 351 CCGCGG CCGC^GG
MaeI 1345 CTAG C^TAG
NcoI 894 CCATGG C^CATGG
NotI 353 GCGGCCGC GC^GGCCGC
PshAI 1132 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 779 TCATGA T^CATGA
RsrII 2830 CGGACCG CG^G[AT]CCG
SacI 2185 GAGCTC GAGCT^C
SacII 351 CCGCGG CCGC^GG
SalI 2955 GTCGAC G^TCGAC
SbfI 3236 CCTGCAGG CCTGCA^GG
SexAI 971 ACCAGGT A^CC[AT]GGT
SfiI 445 GGCCTGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 438 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 3236 CCTGCAGG CCTGCA^GG
XcmI 1428 CCAGATCCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2634 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1672 ACATGT A^C[GA][CT]GT
2688 ACACGT
AlwI 1431 GATCC GGATC(4/5)
3092 GGATC
AspI 863 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1948 GACAGGGTC
BlpI 420 GCTCAGC GC^T[ACGT]AGC
1842 GCTGAGC
Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC
1842 GCTGAGC
BsmBI 414 CGTCTC CGTCTC(1/5)
2472 CGTCTC
Bsp1407I 1044 TGTACA T^GTACA
2479 TGTACA
BsrGI 1044 TGTACA T^GTACA
2479 TGTACA
BssHII 216 GCGCGC G^CGCGC
234 GCGCGC
BstXI 2313 CCAAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2501 CCACAACTCTGG
BstYI 1430 AGATCC [GA]^GATC[CT]
2252 AGATCT
CelII 420 GCTCAGC GC^T[ACGT]AGC
1842 GCTGAGC
EcoNI 1917 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3195 CCTCTGCCAGG
MflI 1430 AGATCC [GA]^GATC[CT]
2252 AGATCT
MseI 824 TTAA T^TAA
830 TTAA
PflFI 863 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1948 GACAGGGTC
PpuMI 1225 AGGTCCC [GA]G^G[AT]CC[CT]
1952 GGGTCCT
SmlI 1318 CTCAAG C^T[CT][GA]AG
2531 CTCAAG
SphI 1400 GCATGC GCATG^C
2996 GCATGC
SspBI 1044 TGTACA T^GTACA
2479 TGTACA
Tru9I 824 TTAA T^TAA
830 TTAA
Tth111I 863 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1948 GACAGGGTC
XhoII 1430 AGATCC [GA]^GATC[CT]
2252 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwNI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcgI BcnI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmaCI XmaI