Restriction map for
pFN21AE0093
(Circular) (Six-base) MAPSORT of: pFN21AE0093.seq Check: 6899 from: 1 to: 7990
pFN21AE0093 7990 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2160 2265 2473 3350 2160
Size: 105 208 877 6800
Fragments arranged by size:
6800 877 208 105
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5800 278
Size: 53 83 186 936 4264 2468
Fragments arranged by size:
4264 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2796 4949 4961 5333 1937
Size: 859 2153 12 372 4594
Fragments arranged by size:
4594 2153 859 372 12
Acc65I G'GTAC_C
Cuts at: 5220 5311 5220
Size: 91 7899
AclI AA'CG_TT
Cuts at: 1563 6117 6490 1563
Size: 4554 373 3063
Fragments arranged by size:
4554 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2270 3682 4105 6053 7437 7705 1632
Size: 638 1412 423 1948 1384 268 1917
Fragments arranged by size:
1948 1917 1412 1384 638 423 268
AfeI AGC'GCT
Cuts at: 5228 5228
Size: 7990
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7793
AflIII A'CryG_T
Cuts at: 1203 3677 4693 6889 7866 1203
Size: 2474 1016 2196 977 1327
Fragments arranged by size:
2474 2196 1327 1016 977
AgeI A'CCGG_T
Cuts at: 6821 6821
Size: 7990
AhdI GACnn_n'nnGTC
Cuts at: 4768 6719 4768
Size: 1951 6039
AleI CACnn'nnGTG
Cuts at: 1166 2901 3414 1166
Size: 1735 513 5742
Fragments arranged by size:
5742 1735 513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3362 3432 1184
Size: 2178 70 5742
Fragments arranged by size:
5742 2178 70
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4391 4423 4391
Size: 32 7958
AlwNI CAG_nnn'CTG
Cuts at: 2433 2821 3342 3439 4179 4428 4530 5733 7305
Size: 388 521 97 740 249 102 1203 1572
Cuts at: 7305 2433
Size: 3118
Fragments arranged by size:
3118 1572 1203 740 521 388 249 102
97
ApaI G_GGCC'C
Cuts at: 2181 2519 3984 4727 2181
Size: 338 1465 743 5444
Fragments arranged by size:
5444 1465 743 338
ApaLI G'TGCA_C
Cuts at: 2147 4004 4846 6047 7203 2147
Size: 1857 842 1201 1156 2934
Fragments arranged by size:
2934 1857 1201 1156 842
ApoI r'AATT_y
Cuts at: 1493 4329 5299 5525 5677 7786 1493
Size: 2836 970 226 152 2109 1697
Fragments arranged by size:
2836 2109 1697 970 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6544 160
Size: 872 5512 1606
Fragments arranged by size:
5512 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7990
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2174 2316 2386 3751 3923 4131
Size: 73 131 100 142 70 1365 172 208
Cuts at: 4131 4153 4720 4986 5315 1870
Size: 22 567 266 329 4545
Fragments arranged by size:
4545 1365 567 329 266 208 172 142
131 100 73 70 22
BamHI G'GATC_C
Cuts at: 5320 5687 5320
Size: 367 7623
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2352 2438 2895 3501
Size: 325 217 627 228 337 86 457 606
Cuts at: 3501 3557 3582 3653 3755 5019 5220 5311 6766
Size: 56 25 71 102 1264 201 91 1455
Cuts at: 6766 618
Size: 1842
Fragments arranged by size:
1842 1455 1264 627 606 457 337 325
228 217 201 102 91 86 71 56
25
BanII G_rGCy'C
Cuts at: 1391 2181 2519 3647 3694 3854 3984 4194 4557
Size: 790 338 1128 47 160 130 210 363
Cuts at: 4557 4619 4727 5309 1391
Size: 62 108 582 4072
Fragments arranged by size:
4072 1128 790 582 363 338 210 160
130 108 62 47
BbeI G_GCGC'C
Cuts at: 2019 2356 2442 3657 5023 2019
Size: 337 86 1215 1366 4986
Fragments arranged by size:
4986 1366 1215 337 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3141 928
Size: 971 1242 5777
Fragments arranged by size:
5777 1242 971
BbvCI CC'TCA_GC
Cuts at: 2580 2580
Size: 7990
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2068 2102 4456 4490 4678 4712 6181
Size: 34 131 34 2354 34 188 34 1469
Cuts at: 6181 6215 1903
Size: 34 3678
Fragments arranged by size:
3678 2354 1469 188 131 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5882 7092 5882
Size: 1210 6780
BclI T'GATC_A
Cuts at: 1548 2541 1548
Size: 993 6997
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2185 2409 2457
Size: 107 122 71 631 605 513 224 48
Cuts at: 2457 3651 4898 6601 136
Size: 1194 1247 1703 1525
Fragments arranged by size:
1703 1525 1247 1194 631 605 513 224
122 107 71 48
BglII A'GATC_T
Cuts at: 4257 7985 4257
Size: 3728 4262
BlpI GC'TnA_GC
Cuts at: 2426 3848 5407 2426
Size: 1422 1559 5009
Fragments arranged by size:
5009 1559 1422
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2151 2181 2519 2898 3984 4008 4727
Size: 291 70 30 338 379 1086 24 719
Cuts at: 4727 4850 5039 6051 7207 1790
Size: 123 189 1012 1156 2573
Fragments arranged by size:
2573 1156 1086 1012 719 379 338 291
189 123 70 30 24
BmgBI CAC'GTC
Cuts at: 1318 2107 2146 2637 4696 1318
Size: 789 39 491 2059 4612
Fragments arranged by size:
4612 2059 789 491 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4947 5721 6679
Size: 416 53 83 399 24 3530 774 958
Cuts at: 6679 442
Size: 1753
Fragments arranged by size:
3530 1753 958 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7990
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4276 4930 4276
Size: 654 7336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2936 3661 3679 3756 6650 1239
Size: 1697 725 18 77 2894 2579
Fragments arranged by size:
2894 2579 1697 725 77 18
Bpu10I CC'TnA_GC
Cuts at: 2580 2595 2580
Size: 15 7975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3308 4521 6115 6980 7278 3308
Size: 1213 1594 865 298 4020
Fragments arranged by size:
4020 1594 1213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2584 2702 3149 5151 6653 882
Size: 617 1085 118 447 2002 1502 2219
Fragments arranged by size:
2219 2002 1502 1085 617 447 118
BsaAI yAC'GTr
Cuts at: 493 1204 4953 5781 493
Size: 711 3749 828 2702
Fragments arranged by size:
3749 2702 828 711
BsaBI GATnn'nnATC
Cuts at: 7984 7984
Size: 7990
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2016 2194 2353 2439
Size: 53 83 186 936 483 178 159 86
Cuts at: 2439 2727 3611 3654 4784 5020 5797 6179 6814
Size: 288 884 43 1130 236 777 382 635
Cuts at: 6814 275
Size: 1451
Fragments arranged by size:
1451 1130 936 884 777 635 483 382
288 236 186 178 159 86 83 53
43
BsaWI w'CCGG_w
Cuts at: 6423 6821 7095 7242 7653 6423
Size: 398 274 147 411 6760
Fragments arranged by size:
6760 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 3240 3270 1178
Size: 30 165 30 993 30 814 30 5898
Fragments arranged by size:
5898 993 814 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2957 2967 4910 1186
Size: 1162 609 10 1943 4266
Fragments arranged by size:
4266 1943 1162 609 10
BseYI C'CCAG_C
Cuts at: 2209 2567 3175 3809 4428 7193 7689 2209
Size: 358 608 634 619 2765 496 2510
Fragments arranged by size:
2765 2510 634 619 608 496 358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2759 3124 3688 4274 4459 4466
Size: 573 501 522 365 564 586 185 7
Cuts at: 4466 4763 5015 1163
Size: 297 252 4138
Fragments arranged by size:
4138 586 573 564 522 501 365 297
252 185 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 2362 3844 4733 5440 6201
Size: 275 186 102 259 1482 889 707 761
Cuts at: 6201 7229 1540
Size: 1028 2301
Fragments arranged by size:
2301 1482 1028 889 761 707 275 259
186 102
BsiHKAI G_wGCw'C
Cuts at: 2121 2151 2400 2700 4008 4194 4805 4850 4958
Size: 30 249 300 1308 186 611 45 108
Cuts at: 4958 6051 6136 7207 2121
Size: 1093 85 1071 2904
Fragments arranged by size:
2904 1308 1093 1071 611 300 249 186
108 85 45 30
BsmI GAATG_Cn'
Cuts at: 3457 5501 5594 3457
Size: 2044 93 5853
Fragments arranged by size:
5853 2044 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2425 4483 2425
Size: 2058 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2121 2151 2181 2400 2519 2700
Size: 399 291 40 30 30 219 119 181
Cuts at: 2700 2898 3647 3694 3854 3984 4008 4194 4557
Size: 198 749 47 160 130 24 186 363
Cuts at: 4557 4619 4727 4805 4850 4958 5039 5309 6051
Size: 62 108 78 45 108 81 270 742
Cuts at: 6051 6136 7207 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 363 291 270
219 198 186 181 160 130 119 108
108 85 81 78 62 47 45 40
30 30 24
BspEI T'CCGG_A
Cuts at: 7653 7653
Size: 7990
BspHI T'CATG_A
Cuts at: 2784 5879 7609 2784
Size: 3095 1730 3165
Fragments arranged by size:
3165 3095 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 2265 2310 2473 3219 3350 3795 4106
Size: 1316 105 45 163 746 131 445 311
Cuts at: 4106 4673 4973 5249 5345 844
Size: 567 300 276 96 3489
Fragments arranged by size:
3489 1316 746 567 445 311 300 276
163 131 105 96 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2668 2908 5877 1394
Size: 429 209 636 240 2969 3507
Fragments arranged by size:
3507 2969 636 429 240 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 6485 6659 65
Size: 1183 5237 174 1396
Fragments arranged by size:
5237 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2260 2444 3843 4732 6634 6821 1700
Size: 110 450 184 1399 889 1902 187 2869
Fragments arranged by size:
2869 1902 1399 889 450 187 184 110
BsrGI T'GTAC_A
Cuts at: 96 3049 4484 96
Size: 2953 1435 3602
Fragments arranged by size:
3602 2953 1435
BssHII G'CGCG_C
Cuts at: 1925 2221 2239 1925
Size: 296 18 7676
Fragments arranged by size:
7676 296 18
BssSI C'ACGA_G
Cuts at: 4480 4879 5154 6050 7062 4480
Size: 399 275 896 1012 5408
Fragments arranged by size:
5408 1012 896 399 275
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3397 3747 4098 5002 5034 794
Size: 2603 350 351 904 32 3750
Fragments arranged by size:
3750 2603 904 351 350 32
BstBI TT'CG_AA
Cuts at: 6794 6794
Size: 7990
BstEII G'GTnAC_C
Cuts at: 1184 4660 1184
Size: 3476 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 4325 4513 4325
Size: 188 7802
BstYI r'GATC_y
Cuts at: 1972 3435 4257 5320 5687 6072 6089 7541 7627
Size: 1463 822 1063 367 385 17 1452 86
Cuts at: 7627 7639 7985 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1463 1452 1063 822 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2797 2797
Size: 7990
BtgI C'CryG_G
Cuts at: 513 1065 2123 2356 2746 2899 3647 3879 4610
Size: 552 1058 233 390 153 748 232 731
Cuts at: 4610 4839 5023 513
Size: 229 184 3480
Fragments arranged by size:
3480 1058 748 731 552 390 233 232
229 184 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3148 487
Size: 683 56 1922 5329
Fragments arranged by size:
5329 1922 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3434 3745 3833 3938 4362 4447 4454 4746
Size: 322 311 88 105 424 85 7 292
Cuts at: 4746 5003 5506 6300 6320 3112
Size: 257 503 794 20 4782
Fragments arranged by size:
4782 794 503 424 322 311 292 257
105 88 85 20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7990
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3200 3235 529
Size: 35 2636 35 5284
Fragments arranged by size:
5284 2636 35 35
DraI TTT'AAA
Cuts at: 5294 5294
Size: 7990
DraIII CAC_nnn'GTG
Cuts at: 2704 3564 4108 2704
Size: 860 544 6586
Fragments arranged by size:
6586 860 544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6997 809
Size: 507 5681 1802
Fragments arranged by size:
5681 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2359 2401
Size: 54 1425 50 275 18 270 259 42
Cuts at: 2401 2721 3948 4730 5437 6326 7955 8
Size: 320 1227 782 707 889 1629 43
Fragments arranged by size:
1629 1425 1227 889 782 707 320 275
270 259 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 2359 4730 5437 1537
Size: 275 288 259 2371 707 4090
Fragments arranged by size:
4090 2371 707 288 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 5920 1353
Size: 4567 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4470 6577 6951 7097 387
Size: 1064 138 2881 2107 374 146 1280
Fragments arranged by size:
2881 2107 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4192 4192
Size: 7990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2936 3661 3679 3682 3756 4105
Size: 393 638 666 725 18 3 74 349
Cuts at: 4105 6053 6650 7437 7705 1239
Size: 1948 597 787 268 1524
Fragments arranged by size:
1948 1524 787 725 666 638 597 393
349 268 74 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3926 5204 3926
Size: 1278 6712
EcoO109I rG'GnC_Cy
Cuts at: 2178 2515 3231 3958 3981 4724 4975 2178
Size: 337 716 727 23 743 251 5193
Fragments arranged by size:
5193 743 727 716 337 251 23
EcoRI G'AATT_C
Cuts at: 4329 5299 5677 4329
Size: 970 378 6642
Fragments arranged by size:
6642 970 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4135 4135
Size: 7990
FspI TGC'GCA
Cuts at: 6496 6496
Size: 7990
HaeII r_GCGC'y
Cuts at: 1678 2019 2356 2433 2442 3657 5023 5230 7137
Size: 341 337 77 9 1215 1366 207 1907
Cuts at: 7137 1678
Size: 2531
Fragments arranged by size:
2531 1907 1366 1215 341 337 207 77
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2911 2943 3197 3200 3229
Size: 32 135 32 1339 32 254 3 29
Cuts at: 3229 3232 4059 4091 4495 4527 4754 4786 6631
Size: 3 827 32 404 32 227 32 1845
Cuts at: 6631 6663 6705 6737 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1845 1339 827 404 254 227 135
42 32 32 32 32 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3889 4498 4962 5334 5580 669
Size: 988 281 1951 609 464 372 246 3079
Fragments arranged by size:
3079 1951 988 609 464 372 281 246
HindIII A'AGCT_T
Cuts at: 748 2852 748
Size: 2104 5886
HpaI GTT'AAC
Cuts at: 5580 5580
Size: 7990
KasI G'GCGC_C
Cuts at: 2015 2352 2438 3653 5019 2015
Size: 337 86 1215 1366 4986
Fragments arranged by size:
4986 1366 1215 337 86
KpnI G_GTAC'C
Cuts at: 5224 5315 5224
Size: 91 7899
MfeI C'AATT_G
Cuts at: 5589 5589
Size: 7990
MluI A'CGCG_T
Cuts at: 7866 7866
Size: 7990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3058 7104 7288 1305
Size: 1753 4046 184 2007
Fragments arranged by size:
4046 2007 1753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6168
Fragments arranged by size:
6168 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 2843 2901 3057 3204 3414 5948
Size: 648 1623 54 58 156 147 210 2534
Cuts at: 5948 6307 6466 518
Size: 359 159 2042
Fragments arranged by size:
2534 2042 1623 648 359 210 159 156
147 58 54
MspA1I CmG'CkG
Cuts at: 1606 2084 2161 2290 2358 4083 4288 4425 4678
Size: 478 77 129 68 1725 205 137 253
Cuts at: 4678 4812 5406 6083 7231 7476 1606
Size: 134 594 677 1148 245 2120
Fragments arranged by size:
2120 1725 1148 677 594 478 253 245
205 137 134 129 77 68
NaeI GCC'GGC
Cuts at: 2262 2446 4734 2262
Size: 184 2288 5518
Fragments arranged by size:
5518 2288 184
NarI GG'CG_CC
Cuts at: 2016 2353 2439 3654 5020 2016
Size: 337 86 1215 1366 4986
Fragments arranged by size:
4986 1366 1215 337 86
NcoI C'CATG_G
Cuts at: 513 1065 2899 513
Size: 552 1834 5604
Fragments arranged by size:
5604 1834 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7990
NgoMIV G'CCGG_C
Cuts at: 2260 2444 4732 2260
Size: 184 2288 5518
Fragments arranged by size:
5518 2288 184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7990
NotI GC'GGCC_GC
Cuts at: 2359 5437 2359
Size: 3078 4912
NspI r_CATG'y
Cuts at: 1132 1229 3409 3629 3667 3681 3773 5005 5215
Size: 97 2180 220 38 14 92 1232 210
Cuts at: 5215 5348 6893 1132
Size: 133 1545 2229
Fragments arranged by size:
2229 2180 1545 1232 220 210 133 97
92 38 14
PasI CC'CwG_GG
Cuts at: 1348 2512 3728 1348
Size: 1164 1216 5610
Fragments arranged by size:
5610 1216 1164
PciI A'CATG_T
Cuts at: 3677 6889 3677
Size: 3212 4778
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 2821 3175 3187 1108
Size: 1417 296 354 12 5911
Fragments arranged by size:
5911 1417 354 296 12
PfoI T'CCnGG_A
Cuts at: 3618 3816 3960 3618
Size: 198 144 7648
Fragments arranged by size:
7648 198 144
PmeI GTTT'AAAC
Cuts at: 5294 5294
Size: 7990
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6059 6091 7572 7604 6059
Size: 32 1481 32 6445
Fragments arranged by size:
6445 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3231 3958 3231
Size: 727 7263
PshAI GACnn'nnGTC
Cuts at: 1613 3141 1613
Size: 1528 6462
PsiI TTA'TAA
Cuts at: 5560 5560
Size: 7990
PspOMI G'GGCC_C
Cuts at: 2177 2515 3980 4723 2177
Size: 338 1465 743 5444
Fragments arranged by size:
5444 1465 743 338
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 7958
PstI C_TGCA'G
Cuts at: 830 3299 3467 4054 4082 4683 4983 5246 5342
Size: 2469 168 587 28 601 300 263 96
Cuts at: 5342 830
Size: 3478
Fragments arranged by size:
3478 2469 601 587 300 263 168 96
28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7990
PvuII CAG'CTG
Cuts at: 2084 2290 4678 4812 2084
Size: 206 2388 134 5262
Fragments arranged by size:
5262 2388 206 134
RsrII CG'GwC_CG
Cuts at: 4836 4836
Size: 7990
SacI G_AGCT'C
Cuts at: 4194 4194
Size: 7990
SacII CC_GC'GG
Cuts at: 2359 2359
Size: 7990
SalI G'TCGA_C
Cuts at: 1936 4960 5332 1936
Size: 3024 372 4594
Fragments arranged by size:
4594 3024 372
SbfI CC_TGCA'GG
Cuts at: 5246 5342 5246
Size: 96 7894
ScaI AGT'ACT
Cuts at: 6238 7950 6238
Size: 1712 6278
SexAI A'CCwGG_T
Cuts at: 2976 2976
Size: 7990
SfcI C'TryA_G
Cuts at: 826 1045 3295 3412 3463 4050 4078 4679 4979
Size: 219 2250 117 51 587 28 601 300
Cuts at: 4979 5062 5242 5338 6473 7154 7345 826
Size: 83 180 96 1135 681 191 1471
Fragments arranged by size:
2250 1471 1135 681 601 587 300 219
191 180 117 96 83 51 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 2457 2457
Size: 7990
SfoI GGC'GCC
Cuts at: 2017 2354 2440 3655 5021 2017
Size: 337 86 1215 1366 4986
Fragments arranged by size:
4986 1366 1215 337 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7990
SgrAI Cr'CCGG_yG
Cuts at: 1700 2444 1700
Size: 744 7246
SmaI CCC'GGG
Cuts at: 1872 2076 2176 3753 4133 4722 4988 5317 1872
Size: 204 100 1577 380 589 266 329 4545
Fragments arranged by size:
4545 1577 589 380 329 266 204 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 4536 6094 6995 7257 820
Size: 197 926 1380 1213 1558 901 262 1553
Fragments arranged by size:
1558 1553 1380 1213 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5781 493
Size: 5288 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7990
SphI G_CATG'C
Cuts at: 1132 3409 5005 5348 1132
Size: 2277 1596 343 3774
Fragments arranged by size:
3774 2277 1596 343
SrfI GCCC'GGGC
Cuts at: 4722 4722
Size: 7990
SspI AAT'ATT
Cuts at: 5 52 5914 5
Size: 47 5862 2081
Fragments arranged by size:
5862 2081 47
StuI AGG'CCT
Cuts at: 1517 2647 3867 4901 1517
Size: 1130 1220 1034 4606
Fragments arranged by size:
4606 1220 1130 1034
StyI C'CwwG_G
Cuts at: 513 1065 2899 3798 4266 5429 513
Size: 552 1834 899 468 1163 3074
Fragments arranged by size:
3074 1834 1163 899 552 468
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3244 4588 6017 6034 6187 6372 6817
Size: 1255 1664 1344 1429 17 153 185 445
Cuts at: 6817 325
Size: 1498
Fragments arranged by size:
1664 1498 1429 1344 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3049 4484 6236
Size: 274 80 33 51 1443 1072 1435 1752
Cuts at: 6236 7948 96
Size: 1712 138
Fragments arranged by size:
1752 1712 1443 1435 1072 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2396 2428 3050 3082 3240 3259 3272 3291 3812
Size: 32 622 32 158 19 13 19 521
Cuts at: 3812 3844 5201 5233 7950 7982 2396
Size: 32 1357 32 2717 32 2404
Fragments arranged by size:
2717 2404 1357 622 521 158 32 32
32 32 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 1316 2871 3956 1316
Size: 1555 1085 5350
Fragments arranged by size:
5350 1555 1085
XbaI T'CTAG_A
Cuts at: 5326 5326
Size: 7990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3440 3440
Size: 7990
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7990
XmaI C'CCGG_G
Cuts at: 1870 2074 2174 3751 4131 4720 4986 5315 1870
Size: 204 100 1577 380 589 266 329 4545
Fragments arranged by size:
4545 1577 589 380 329 266 204 100
XmnI GAAnn'nnTTC
Cuts at: 4643 6119 4643
Size: 1476 6514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5798 276
Size: 53 83 186 936 4264 2468
Fragments arranged by size:
4264 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI Bsu36I EcoRV FseI
FspAI NruI NsiI PacI PmlI PspXI SanDI SapI
SwaI