Restriction map for
pFN21AE0093
(Linear) (Six-base) MAPSORT of: KIEE0093.seq Check: 9987 from: 1 to: 3285
KIEE0093.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 155 260 468 1345 3285
Size: 155 105 208 877 1940
Fragments arranged by size:
1940 877 208 155 105
AccI GT'mk_AC
Cuts at: 0 791 2944 2956 3285
Size: 791 2153 12 329
Fragments arranged by size:
2153 791 329 12
Acc65I G'GTAC_C
Cuts at: 0 3215 3285
Size: 3215 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1677 2100 3285
Size: 265 1412 423 1185
Fragments arranged by size:
1412 1185 423 265
AfeI AGC'GCT
Cuts at: 0 3223 3285
Size: 3223 62
AflIII A'CryG_T
Cuts at: 0 1672 2688 3285
Size: 1672 1016 597
Fragments arranged by size:
1672 1016 597
AhdI GACnn_n'nnGTC
Cuts at: 0 2763 3285
Size: 2763 522
AleI CACnn'nnGTG
Cuts at: 0 896 1409 3285
Size: 896 513 1876
Fragments arranged by size:
1876 896 513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 1427 3285
Size: 1357 70 1858
Fragments arranged by size:
1858 1357 70
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2386 2418 3285
Size: 2386 32 867
Fragments arranged by size:
2386 867 32
AlwNI CAG_nnn'CTG
Cuts at: 0 428 816 1337 1434 2174 2423 2525 3285
Size: 428 388 521 97 740 249 102 760
Fragments arranged by size:
760 740 521 428 388 249 102 97
ApaI G_GGCC'C
Cuts at: 0 176 514 1979 2722 3285
Size: 176 338 1465 743 563
Fragments arranged by size:
1465 743 563 338 176
ApaLI G'TGCA_C
Cuts at: 0 142 1999 2841 3285
Size: 142 1857 842 444
Fragments arranged by size:
1857 842 444 142
ApoI r'AATT_y
Cuts at: 0 2324 3285
Size: 2324 961
AvaI C'yCGr_G
Cuts at: 0 69 169 311 381 1746 1918 2126 2148
Size: 69 100 142 70 1365 172 208 22
Cuts at: 2148 2715 2981 3285
Size: 567 266 304
Fragments arranged by size:
1365 567 304 266 208 172 142 100
70 69 22
BanI G'GyrC_C
Cuts at: 0 10 347 433 890 1496 1552 1577 1648
Size: 10 337 86 457 606 56 25 71
Cuts at: 1648 1750 3014 3215 3285
Size: 102 1264 201 70
Fragments arranged by size:
1264 606 457 337 201 102 86 71
70 56 25 10
BanII G_rGCy'C
Cuts at: 0 176 514 1642 1689 1849 1979 2189 2552
Size: 176 338 1128 47 160 130 210 363
Cuts at: 2552 2614 2722 3285
Size: 62 108 563
Fragments arranged by size:
1128 563 363 338 210 176 160 130
108 62 47
BbeI G_GCGC'C
Cuts at: 0 14 351 437 1652 3018 3285
Size: 14 337 86 1215 1366 267
Fragments arranged by size:
1366 1215 337 267 86 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 3285
Size: 1136 2149
BbvCI CC'TCA_GC
Cuts at: 0 575 3285
Size: 575 2710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 63 97 2451 2485 2673 2707 3285
Size: 63 34 2354 34 188 34 578
Fragments arranged by size:
2354 578 188 63 34 34 34
BclI T'GATC_A
Cuts at: 0 536 3285
Size: 536 2749
BglI GCCn_nnn'nGGC
Cuts at: 0 180 404 452 1646 2893 3285
Size: 180 224 48 1194 1247 392
Fragments arranged by size:
1247 1194 392 224 180 48
BglII A'GATC_T
Cuts at: 0 2252 3285
Size: 2252 1033
BlpI GC'TnA_GC
Cuts at: 0 421 1843 3285
Size: 421 1422 1442
Fragments arranged by size:
1442 1422 421
Bme1580I G_kGCm'C
Cuts at: 0 76 146 176 514 893 1979 2003 2722
Size: 76 70 30 338 379 1086 24 719
Cuts at: 2722 2845 3034 3285
Size: 123 189 251
Fragments arranged by size:
1086 719 379 338 251 189 123 76
70 30 24
BmgBI CAC'GTC
Cuts at: 0 102 141 632 2691 3285
Size: 102 39 491 2059 594
Fragments arranged by size:
2059 594 491 102 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 2942 3285
Size: 2942 343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2271 2925 3285
Size: 2271 654 360
Fragments arranged by size:
2271 654 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1656 1674 1751 3285
Size: 931 725 18 77 1534
Fragments arranged by size:
1534 931 725 77 18
Bpu10I CC'TnA_GC
Cuts at: 0 575 590 3285
Size: 575 15 2695
Fragments arranged by size:
2695 575 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 2516 3285
Size: 1303 1213 769
Fragments arranged by size:
1303 1213 769
BsaI GGTCTCn'nnnn_
Cuts at: 0 579 697 1144 3146 3285
Size: 579 118 447 2002 139
Fragments arranged by size:
2002 579 447 139 118
BsaAI yAC'GTr
Cuts at: 0 2948 3285
Size: 2948 337
BsaHI Gr'CG_yC
Cuts at: 0 11 189 348 434 722 1606 1649 2779
Size: 11 178 159 86 288 884 43 1130
Cuts at: 2779 3015 3285
Size: 236 270
Fragments arranged by size:
1130 884 288 270 236 178 159 86
43 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 1235 1265 3285
Size: 391 30 814 30 2020
Fragments arranged by size:
2020 814 391 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 952 962 2905 3285
Size: 343 609 10 1943 380
Fragments arranged by size:
1943 609 380 343 10
BseYI C'CCAG_C
Cuts at: 0 204 562 1170 1804 2423 3285
Size: 204 358 608 634 619 862
Fragments arranged by size:
862 634 619 608 358 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 754 1119 1683 2269 2454 2461 2758
Size: 232 522 365 564 586 185 7 297
Cuts at: 2758 3010 3285
Size: 252 275
Fragments arranged by size:
586 564 522 365 297 275 252 232
185 7
BsiEI CG_ry'CG
Cuts at: 0 98 357 1839 2728 3285
Size: 98 259 1482 889 557
Fragments arranged by size:
1482 889 557 259 98
BsiHKAI G_wGCw'C
Cuts at: 0 116 146 395 695 2003 2189 2800 2845
Size: 116 30 249 300 1308 186 611 45
Cuts at: 2845 2953 3285
Size: 108 332
Fragments arranged by size:
1308 611 332 300 249 186 116 108
45 30
BsmI GAATG_Cn'
Cuts at: 0 1452 3285
Size: 1452 1833
BsmBI CGTCTCn'nnnn_
Cuts at: 0 420 2478 3285
Size: 420 2058 807
Fragments arranged by size:
2058 807 420
Bsp1286I G_dGCh'C
Cuts at: 0 76 116 146 176 395 514 695 893
Size: 76 40 30 30 219 119 181 198
Cuts at: 893 1642 1689 1849 1979 2003 2189 2552 2614
Size: 749 47 160 130 24 186 363 62
Cuts at: 2614 2722 2800 2845 2953 3034 3285
Size: 108 78 45 108 81 251
Fragments arranged by size:
749 363 251 219 198 186 181 160
130 119 108 108 81 78 76 62
47 45 40 30 30 24
BspHI T'CATG_A
Cuts at: 0 779 3285
Size: 779 2506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 260 305 468 1214 1345 1790 2101
Size: 155 105 45 163 746 131 445 311
Cuts at: 2101 2668 2968 3244 3285
Size: 567 300 276 41
Fragments arranged by size:
746 567 445 311 300 276 163 155
131 105 45 41
BsrBI CCG'CTC
Cuts at: 0 27 663 903 3285
Size: 27 636 240 2382
Fragments arranged by size:
2382 636 240 27
BsrFI r'CCGG_y
Cuts at: 0 255 439 1838 2727 3285
Size: 255 184 1399 889 558
Fragments arranged by size:
1399 889 558 255 184
BsrGI T'GTAC_A
Cuts at: 0 1044 2479 3285
Size: 1044 1435 806
Fragments arranged by size:
1435 1044 806
BssHII G'CGCG_C
Cuts at: 0 216 234 3285
Size: 216 18 3051
Fragments arranged by size:
3051 216 18
BssSI C'ACGA_G
Cuts at: 0 2475 2874 3149 3285
Size: 2475 399 275 136
Fragments arranged by size:
2475 399 275 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1392 1742 2093 2997 3029 3285
Size: 1392 350 351 904 32 256
Fragments arranged by size:
1392 904 351 350 256 32
BstEII G'GTnAC_C
Cuts at: 0 2655 3285
Size: 2655 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2320 2508 3285
Size: 2320 188 777
Fragments arranged by size:
2320 777 188
BstYI r'GATC_y
Cuts at: 0 1430 2252 3285
Size: 1430 822 1033
Fragments arranged by size:
1430 1033 822
BstZ17I GTA'TAC
Cuts at: 0 792 3285
Size: 792 2493
BtgI C'CryG_G
Cuts at: 0 118 351 741 894 1642 1874 2605 2834
Size: 118 233 390 153 748 232 731 229
Cuts at: 2834 3018 3285
Size: 184 267
Fragments arranged by size:
748 731 390 267 233 232 229 184
153 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1143 3285
Size: 1143 2142
BtsI GCAGTG_nn'
Cuts at: 0 1107 1429 1740 1828 1933 2357 2442 2449
Size: 1107 322 311 88 105 424 85 7
Cuts at: 2449 2741 2998 3285
Size: 292 257 287
Fragments arranged by size:
1107 424 322 311 292 287 257 105
88 85 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1195 1230 3285
Size: 1195 35 2055
Fragments arranged by size:
2055 1195 35
DraIII CAC_nnn'GTG
Cuts at: 0 699 1559 2103 3285
Size: 699 860 544 1182
Fragments arranged by size:
1182 860 699 544
EaeI y'GGCC_r
Cuts at: 0 95 354 396 716 1943 2725 3285
Size: 95 259 42 320 1227 782 560
Fragments arranged by size:
1227 782 560 320 259 95 42
EagI C'GGCC_G
Cuts at: 0 95 354 2725 3285
Size: 95 259 2371 560
Fragments arranged by size:
2371 560 259 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2465 3285
Size: 2465 820
EcoICRI GAG'CTC
Cuts at: 0 2187 3285
Size: 2187 1098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 931 1656 1674 1677 1751 2100 3285
Size: 265 666 725 18 3 74 349 1185
Fragments arranged by size:
1185 725 666 349 265 74 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1921 3199 3285
Size: 1921 1278 86
Fragments arranged by size:
1921 1278 86
EcoO109I rG'GnC_Cy
Cuts at: 0 173 510 1226 1953 1976 2719 2970 3285
Size: 173 337 716 727 23 743 251 315
Fragments arranged by size:
743 727 716 337 315 251 173 23
EcoRI G'AATT_C
Cuts at: 0 2324 3285
Size: 2324 961
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2130 3285
Size: 2130 1155
HaeII r_GCGC'y
Cuts at: 0 14 351 428 437 1652 3018 3225 3285
Size: 14 337 77 9 1215 1366 207 60
Fragments arranged by size:
1366 1215 337 207 77 60 14 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 1192 1195 1224 1227 2054 2086
Size: 906 32 254 3 29 3 827 32
Cuts at: 2086 2490 2522 2749 2781 3285
Size: 404 32 227 32 504
Fragments arranged by size:
906 827 504 404 254 227 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 1884 2493 2957 3285
Size: 1884 609 464 328
Fragments arranged by size:
1884 609 464 328
HindIII A'AGCT_T
Cuts at: 0 847 3285
Size: 847 2438
KasI G'GCGC_C
Cuts at: 0 10 347 433 1648 3014 3285
Size: 10 337 86 1215 1366 271
Fragments arranged by size:
1366 1215 337 271 86 10
KpnI G_GTAC'C
Cuts at: 0 3219 3285
Size: 3219 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1053 3285
Size: 1053 2232
MslI CAynn'nnrTG
Cuts at: 0 784 838 896 1052 1199 1409 3285
Size: 784 54 58 156 147 210 1876
Fragments arranged by size:
1876 784 210 156 147 58 54
MspA1I CmG'CkG
Cuts at: 0 79 156 285 353 2078 2283 2420 2673
Size: 79 77 129 68 1725 205 137 253
Cuts at: 2673 2807 3285
Size: 134 478
Fragments arranged by size:
1725 478 253 205 137 134 129 79
77 68
NaeI GCC'GGC
Cuts at: 0 257 441 2729 3285
Size: 257 184 2288 556
Fragments arranged by size:
2288 556 257 184
NarI GG'CG_CC
Cuts at: 0 11 348 434 1649 3015 3285
Size: 11 337 86 1215 1366 270
Fragments arranged by size:
1366 1215 337 270 86 11
NcoI C'CATG_G
Cuts at: 0 894 3285
Size: 894 2391
NgoMIV G'CCGG_C
Cuts at: 0 255 439 2727 3285
Size: 255 184 2288 558
Fragments arranged by size:
2288 558 255 184
NotI GC'GGCC_GC
Cuts at: 0 354 3285
Size: 354 2931
NspI r_CATG'y
Cuts at: 0 1404 1624 1662 1676 1768 3000 3210 3285
Size: 1404 220 38 14 92 1232 210 75
Fragments arranged by size:
1404 1232 220 210 92 75 38 14
PasI CC'CwG_GG
Cuts at: 0 507 1723 3285
Size: 507 1216 1562
Fragments arranged by size:
1562 1216 507
PciI A'CATG_T
Cuts at: 0 1672 3285
Size: 1672 1613
PflMI CCAn_nnn'nTGG
Cuts at: 0 520 816 1170 1182 3285
Size: 520 296 354 12 2103
Fragments arranged by size:
2103 520 354 296 12
PfoI T'CCnGG_A
Cuts at: 0 1613 1811 1955 3285
Size: 1613 198 144 1330
Fragments arranged by size:
1613 1330 198 144
PpuMI rG'GwC_Cy
Cuts at: 0 1226 1953 3285
Size: 1226 727 1332
Fragments arranged by size:
1332 1226 727
PshAI GACnn'nnGTC
Cuts at: 0 1136 3285
Size: 1136 2149
PspOMI G'GGCC_C
Cuts at: 0 172 510 1975 2718 3285
Size: 172 338 1465 743 567
Fragments arranged by size:
1465 743 567 338 172
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 775 807 3285
Size: 775 32 2478
Fragments arranged by size:
2478 775 32
PstI C_TGCA'G
Cuts at: 0 1294 1462 2049 2077 2678 2978 3241 3285
Size: 1294 168 587 28 601 300 263 44
Fragments arranged by size:
1294 601 587 300 263 168 44 28
PvuII CAG'CTG
Cuts at: 0 79 285 2673 2807 3285
Size: 79 206 2388 134 478
Fragments arranged by size:
2388 478 206 134 79
RsrII CG'GwC_CG
Cuts at: 0 2831 3285
Size: 2831 454
SacI G_AGCT'C
Cuts at: 0 2189 3285
Size: 2189 1096
SacII CC_GC'GG
Cuts at: 0 354 3285
Size: 354 2931
SalI G'TCGA_C
Cuts at: 0 2955 3285
Size: 2955 330
SbfI CC_TGCA'GG
Cuts at: 0 3241 3285
Size: 3241 44
SexAI A'CCwGG_T
Cuts at: 0 971 3285
Size: 971 2314
SfcI C'TryA_G
Cuts at: 0 1290 1407 1458 2045 2073 2674 2974 3057
Size: 1290 117 51 587 28 601 300 83
Cuts at: 3057 3237 3285
Size: 180 48
Fragments arranged by size:
1290 601 587 300 180 117 83 51
48 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 452 3285
Size: 452 2833
SfoI GGC'GCC
Cuts at: 0 12 349 435 1650 3016 3285
Size: 12 337 86 1215 1366 269
Fragments arranged by size:
1366 1215 337 269 86 12
SgrAI Cr'CCGG_yG
Cuts at: 0 439 3285
Size: 439 2846
SmaI CCC'GGG
Cuts at: 0 71 171 1748 2128 2717 2983 3285
Size: 71 100 1577 380 589 266 302
Fragments arranged by size:
1577 589 380 302 266 100 71
SmlI C'TyrA_G
Cuts at: 0 1318 2531 3285
Size: 1318 1213 754
Fragments arranged by size:
1318 1213 754
SphI G_CATG'C
Cuts at: 0 1404 3000 3285
Size: 1404 1596 285
Fragments arranged by size:
1596 1404 285
SrfI GCCC'GGGC
Cuts at: 0 2717 3285
Size: 2717 568
StuI AGG'CCT
Cuts at: 0 642 1862 2896 3285
Size: 642 1220 1034 389
Fragments arranged by size:
1220 1034 642 389
StyI C'CwwG_G
Cuts at: 0 894 1793 2261 3285
Size: 894 899 468 1024
Fragments arranged by size:
1024 899 894 468
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1239 2583 3285
Size: 1239 1344 702
Fragments arranged by size:
1344 1239 702
TatI w'GTAC_w
Cuts at: 0 1044 2479 3285
Size: 1044 1435 806
Fragments arranged by size:
1435 1044 806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 1045 1077 1235 1254 1267 1286
Size: 391 32 622 32 158 19 13 19
Cuts at: 1286 1807 1839 3196 3228 3285
Size: 521 32 1357 32 57
Fragments arranged by size:
1357 622 521 391 158 57 32 32
32 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 0 866 1951 3285
Size: 866 1085 1334
Fragments arranged by size:
1334 1085 866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1435 3285
Size: 1435 1850
XmaI C'CCGG_G
Cuts at: 0 69 169 1746 2126 2715 2981 3285
Size: 69 100 1577 380 589 266 304
Fragments arranged by size:
1577 589 380 304 266 100 69
XmnI GAAnn'nnTTC
Cuts at: 0 2638 3285
Size: 2638 647
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PflMI
PfoI PpuMI PshAI PspOMI PsrI PstI PvuII RsrII
SacI SacII SalI SbfI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BfrBI BmtI BsaBI BsaWI BsiWI
BspEI BsrDI BstBI Bsu36I ClaI DraI DrdI EarI
EcoRV FseI FspI FspAI HpaI MfeI MluI MscI
NdeI NheI NruI NsiI PacI PmeI PmlI PpiI
PsiI PspXI PvuI SanDI SapI ScaI SgfI SnaBI
SpeI SspI SwaI XbaI XhoI ZraI