Restriction map for
pFN21AE0092
##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspI
BamHI BbrPI BcgI BsaAI BsaBI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoT22I FseI
HindIII HpaI KspI MamI MfeI
MluI MunI MvnI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3365 GACGTC GACGT^C
AccI 1958 GTCGAC GT^[AC][GT]AC
AccIII 3038 TCCGGA T^CCGGA
AflII 3613 CTTAAG C^TTAAG
AhdI 2803 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3560 GGGCCC GGGCC^C
Asp700I 1648 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2803 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 4931 GGCGCC GGCGC^C
BfrI 3613 CTTAAG C^TTAAG
BglI 4812 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2762 AGATCT A^GATCT
BsaWI 3038 TCCGGA [AT]^CCGG[AT]
BseAI 3038 TCCGGA T^CCGGA
BsmBI 1629 GAGACG CGTCTC(1/5)
BspEI 3038 TCCGGA T^CCGGA
BssSI 2516 CACGAG CACGAG(-5/-1)
BstEII 2361 GGTGACC G^GT[ACGT]ACC
BstPI 2361 GGTGACC G^GT[ACGT]ACC
Eam1105I 2803 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2361 GGTGACC G^GT[ACGT]ACC
EcoRI 5191 GAATTC G^AATTC
KasI 4931 GGCGCC G^GCGCC
MroI 3038 TCCGGA T^CCGGA
NarI 4931 GGCGCC GG^CGCC
NcoI 470 CCATGG C^CATGG
PaeR7I 4942 CTCGAG C^TCGAG
SacI 2941 GAGCTC GAGCT^C
SalI 1958 GTCGAC G^TCGAC
SapI 724 GAAGAGC GCTCTTC(1/4)
SbfI 2643 CCTGCAGG CCTGCA^GG
SexAI 1729 ACCTGGT A^CC[AT]GGT
SfoI 4931 GGCGCC GGC^GCC
Sse8387I 2643 CCTGCAGG CCTGCA^GG
XbaI 1554 TCTAGA T^CTAGA
XhoI 4942 CTCGAG C^TCGAG
XmnI 1648 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 522 GAATTT [GA]^AATT[CT]
5191 GAATTC
AflIII 4615 ACATGT A^C[GA][CT]GT
5032 ACATGT
ApoI 522 GAATTT [GA]^AATT[CT]
5191 GAATTC
AviII 1691 TGCGCA TGC^GCA
2873 TGCGCA
AvrII 1119 CCTAGG C^CTAGG
3604 CCTAGG
BlnI 1119 CCTAGG C^CTAGG
3604 CCTAGG
BspHI 1622 TCATGA T^CATGA
4827 TCATGA
BspLU11I 4615 ACATGT A^CATGT
5032 ACATGT
BsrBI 4945 GAGCGG CCGCTC(-3/-3)
5533 GAGCGG
DrdI 2654 GACTCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2692 GACCTGCTGGTC
DsaI 470 CCATGG C^C[GA][CT]GG
5094 CCGTGG
EcoRV 4684 GATATC GAT^ATC
4896 GATATC
FspI 1691 TGCGCA TGC^GCA
2873 TGCGCA
MslI 640 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5383 CACCACAGTG
NaeI 389 GCCGGC GCC^GGC
3032 GCCGGC
NdeI 2840 CATATG CA^TATG
4901 CATATG
NgoMIV 389 GCCGGC G^CCGGC
3032 GCCGGC
NspI 4615 ACATGT [GA]CATG^[CT]
5032 ACATGT
PpuMI 323 AGGACCC [GA]G^G[AT]CC[CT]
2639 GGGTCCT
RcaI 1622 TCATGA T^CATGA
4827 TCATGA
SmaI 3557 CCCGGG CCC^GGG
3589 CCCGGG
XcmI 1562 CCACTGCTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5140 CCAGAAGGAGATTGG
XmaCI 3557 CCCGGG C^CCGGG
3589 CCCGGG
XmaI 3557 CCCGGG C^CCGGG
3589 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwI AlwNI AocI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII