(Circular) (Six-base) MAPSORT of: pFN21AE0092.seq Check: 6510 from: 1 to: 10420 pFN21AE0092 10420 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2065 7174 2065 Size: 5109 5311 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5374 8230 278 Size: 53 83 186 936 3838 2856 2468 Fragments arranged by size: 3838 2856 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3964 7763 1937 Size: 2027 3799 4594 Fragments arranged by size: 4594 3799 2027 Acc65I G'GTAC_C Cuts at: 2462 6804 7491 7741 2462 Size: 4342 687 250 5141 Fragments arranged by size: 5141 4342 687 250 AclI AA'CG_TT Cuts at: 1563 8547 8920 1563 Size: 6984 373 3063 Fragments arranged by size: 6984 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2187 2661 2765 3040 3192 3453 3817 3823 Size: 555 474 104 275 152 261 364 6 Cuts at: 3823 4328 4736 5106 5651 5699 5705 5711 7656 Size: 505 408 370 545 48 6 6 1945 Cuts at: 7656 8483 9867 10135 1632 Size: 827 1384 268 1917 Fragments arranged by size: 1945 1917 1384 827 555 545 505 474 408 370 364 275 268 261 152 104 48 6 6 6 AflII C'TTAA_G Cuts at: 820 1017 5618 820 Size: 197 4601 5622 Fragments arranged by size: 5622 4601 197 AflIII A'CryG_T Cuts at: 1203 6620 7037 9319 10296 1203 Size: 5417 417 2282 977 1327 Fragments arranged by size: 5417 2282 1327 977 417 AgeI A'CCGG_T Cuts at: 9251 9251 Size: 10420 AhdI GACnn_n'nnGTC Cuts at: 4813 9149 4813 Size: 4336 6084 AleI CACnn'nnGTG Cuts at: 1166 7392 1166 Size: 6226 4194 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4935 5330 6504 1184 Size: 3751 395 1174 5100 Fragments arranged by size: 5100 3751 1174 395 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5116 5148 5116 Size: 32 10388 AlwNI CAG_nnn'CTG Cuts at: 3242 3512 4507 5933 6032 8163 9735 3242 Size: 270 995 1426 99 2131 1572 3927 Fragments arranged by size: 3927 2131 1572 1426 995 270 99 ApaI G_GGCC'C Cuts at: 5569 5569 Size: 10420 ApaLI G'TGCA_C Cuts at: 8477 9633 8477 Size: 1156 9264 ApoI r'AATT_y Cuts at: 1493 2527 7196 7729 7955 8107 10216 1493 Size: 1034 4669 533 226 152 2109 1697 Fragments arranged by size: 4669 2109 1697 1034 533 226 152 AseI AT'TA_AT Cuts at: 160 1032 8974 160 Size: 872 7942 1606 Fragments arranged by size: 7942 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10420 AvaI C'yCGr_G Cuts at: 1870 1943 2353 5562 5568 5594 6375 6947 7745 Size: 73 410 3209 6 26 781 572 798 Cuts at: 7745 1870 Size: 4545 Fragments arranged by size: 4545 3209 798 781 572 410 73 26 6 AvrII C'CTAG_G Cuts at: 3124 5609 3124 Size: 2485 7935 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6788 6821 6788 Size: 33 10387 BamHI G'GATC_C Cuts at: 7750 8117 7750 Size: 367 10053 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 2920 6724 6804 6875 Size: 325 217 627 675 458 3804 80 71 Cuts at: 6875 6887 6936 6954 7491 7741 9196 618 Size: 12 49 18 537 250 1455 1842 Fragments arranged by size: 3804 1842 1455 675 627 537 458 325 250 217 80 71 49 18 12 BanII G_rGCy'C Cuts at: 1391 2907 4950 5569 6477 6819 7350 7739 1391 Size: 1516 2043 619 908 342 531 389 4072 Fragments arranged by size: 4072 2043 1516 908 619 531 389 342 BbeI G_GCGC'C Cuts at: 6940 6940 Size: 10420 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2877 2898 3391 3656 6536 6654 928 Size: 971 978 21 493 265 2880 118 4694 Fragments arranged by size: 4694 2880 978 971 493 265 118 21 BbvCI CC'TCA_GC Cuts at: 3424 5709 5795 6057 6454 6729 3424 Size: 2285 86 262 397 275 7115 Fragments arranged by size: 7115 2285 397 275 262 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8611 8645 1903 Size: 34 6674 34 3678 Fragments arranged by size: 6674 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2651 6321 6392 8312 9522 2651 Size: 3670 71 1920 1210 3549 Fragments arranged by size: 3670 3549 1920 1210 71 BclI T'GATC_A Cuts at: 1548 2860 3244 3783 1548 Size: 1312 384 539 8185 Fragments arranged by size: 8185 1312 539 384 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6823 9031 136 Size: 107 122 71 631 605 5151 2208 1525 Fragments arranged by size: 5151 2208 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4767 10415 4767 Size: 5648 4772 BlpI GC'TnA_GC Cuts at: 5679 5822 6860 7179 7837 5679 Size: 143 1038 319 658 8262 Fragments arranged by size: 8262 1038 658 319 143 Bme1580I G_kGCm'C Cuts at: 1790 5569 6880 6890 7656 8481 9637 1790 Size: 3779 1311 10 766 825 1156 2573 Fragments arranged by size: 3779 2573 1311 1156 825 766 10 BmgBI CAC'GTC Cuts at: 1318 3577 1318 Size: 2259 8161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2341 4539 5085 Size: 416 53 83 399 24 924 2198 546 Cuts at: 5085 5389 5783 6231 8151 9109 442 Size: 304 394 448 1920 958 1753 Fragments arranged by size: 2198 1920 1753 958 924 546 448 416 399 394 304 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10420 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 5847 6328 6688 7648 3617 Size: 2230 481 360 960 6389 Fragments arranged by size: 6389 2230 960 481 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2218 2921 3315 3339 3375 3399 3612 4112 Size: 979 703 394 24 36 24 213 500 Cuts at: 4112 5012 5768 6099 6198 6596 6866 7016 9080 Size: 900 756 331 99 398 270 150 2064 Cuts at: 9080 1239 Size: 2579 Fragments arranged by size: 2579 2064 979 900 756 703 500 398 394 331 270 213 150 99 36 24 24 Bpu10I CC'TnA_GC Cuts at: 3424 3794 5709 5795 6057 6313 6454 6729 7547 Size: 370 1915 86 262 256 141 275 818 Cuts at: 7547 3424 Size: 6297 Fragments arranged by size: 6297 1915 818 370 275 262 256 141 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2309 3017 5946 6344 8545 9410 9708 2309 Size: 708 2929 398 2201 865 298 3021 Fragments arranged by size: 3021 2929 2201 865 708 398 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2121 2806 3415 3725 4220 5144 5350 Size: 617 622 685 609 310 495 924 206 Cuts at: 5350 6165 6223 9083 882 Size: 815 58 2860 2219 Fragments arranged by size: 2860 2219 924 815 685 622 617 609 495 310 206 58 BsaAI yAC'GTr Cuts at: 493 1204 8211 493 Size: 711 7007 2702 Fragments arranged by size: 7007 2702 711 BsaBI GATnn'nnATC Cuts at: 10414 10414 Size: 10420 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5371 6937 7285 8227 Size: 53 83 186 936 3838 1566 348 942 Cuts at: 8227 8609 9244 275 Size: 382 635 1451 Fragments arranged by size: 3838 1566 1451 942 936 635 382 348 186 83 53 BsaWI w'CCGG_w Cuts at: 5043 8853 9251 9525 9672 10083 5043 Size: 3810 398 274 147 411 5380 Fragments arranged by size: 5380 3810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3722 3752 3914 3944 4769 Size: 30 165 30 2319 30 162 30 825 Cuts at: 4769 4799 4985 5015 5116 5146 7418 7448 1178 Size: 30 186 30 101 30 2272 30 4150 Fragments arranged by size: 4150 2319 2272 825 186 165 162 101 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 3646 3935 4130 4376 5162 5195 5456 Size: 1035 1425 289 195 246 786 33 261 Cuts at: 5456 5936 6061 6584 7586 7703 1186 Size: 480 125 523 1002 117 3903 Fragments arranged by size: 3903 1425 1035 1002 786 523 480 289 261 246 195 125 117 33 BseYI C'CCAG_C Cuts at: 2133 2258 2435 3231 3307 3436 3505 3532 4606 Size: 125 177 796 76 129 69 27 1074 Cuts at: 4606 4884 4899 4908 5661 5697 5703 5899 6143 Size: 278 15 9 753 36 6 196 244 Cuts at: 6143 6205 6276 6288 7315 7649 7683 9623 10119 Size: 62 71 12 1027 334 34 1940 496 Cuts at: 10119 2133 Size: 2434 Fragments arranged by size: 2434 1940 1074 1027 796 753 496 334 278 244 196 177 129 125 76 71 69 62 36 34 27 15 12 9 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2037 2997 3926 4707 6140 1163 Size: 573 301 960 929 781 1433 5443 Fragments arranged by size: 5443 1433 960 929 781 573 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7870 8631 9659 1540 Size: 275 186 5869 761 1028 2301 Fragments arranged by size: 5869 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3831 4950 6314 6669 8481 8566 9637 3831 Size: 1119 1364 355 1812 85 1071 4614 Fragments arranged by size: 4614 1812 1364 1119 1071 355 85 BsmI GAATG_Cn' Cuts at: 2601 5117 6924 7931 8024 2601 Size: 2516 1807 1007 93 4997 Fragments arranged by size: 4997 2516 1807 1007 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3628 3628 Size: 10420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2907 3831 4950 5569 6314 6477 6669 Size: 399 1117 924 1119 619 745 163 192 Cuts at: 6669 6819 6880 6890 7350 7656 7739 8481 8566 Size: 150 61 10 460 306 83 742 85 Cuts at: 8566 9637 1391 Size: 1071 2174 Fragments arranged by size: 2174 1119 1117 1071 924 745 742 619 460 399 306 192 163 150 85 83 61 10 BspEI T'CCGG_A Cuts at: 5043 10083 5043 Size: 5040 5380 BspHI T'CATG_A Cuts at: 3627 6832 8309 10039 3627 Size: 3205 1477 1730 4008 Fragments arranged by size: 4008 3205 1730 1477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 4707 6496 6816 7174 7775 844 Size: 1221 2642 1789 320 358 601 3489 Fragments arranged by size: 3489 2642 1789 1221 601 358 320 BsrBI CCG'CTC Cuts at: 1394 1823 6952 7540 8307 1394 Size: 429 5129 588 767 3507 Fragments arranged by size: 5129 3507 767 588 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 6007 7005 8915 9089 65 Size: 1183 3290 1469 998 1910 174 1396 Fragments arranged by size: 3290 1910 1469 1396 1183 998 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 5037 7494 9064 9251 1700 Size: 110 584 2643 2457 1570 187 2869 Fragments arranged by size: 2869 2643 2457 1570 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10420 BssSI C'ACGA_G Cuts at: 4521 8480 9492 4521 Size: 3959 1012 5449 Fragments arranged by size: 5449 3959 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2060 3904 4133 4699 5486 7660 794 Size: 1266 1844 229 566 787 2174 3554 Fragments arranged by size: 3554 2174 1844 1266 787 566 229 BstBI TT'CG_AA Cuts at: 9224 9224 Size: 10420 BstEII G'GTnAC_C Cuts at: 1184 4366 1184 Size: 3182 7238 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4736 5161 6139 6205 6751 2011 Size: 2725 425 978 66 546 5680 Fragments arranged by size: 5680 2725 978 546 425 66 BstYI r'GATC_y Cuts at: 1972 2594 3924 4767 4791 4821 6334 7750 8117 Size: 622 1330 843 24 30 1513 1416 367 Cuts at: 8117 8502 8519 9971 10057 10069 10415 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1513 1452 1416 1330 843 622 385 367 346 86 30 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2065 2089 4035 5439 5673 6882 2065 Size: 24 1946 1404 234 1209 5603 Fragments arranged by size: 5603 1946 1404 1209 234 24 BtgI C'CryG_G Cuts at: 513 1065 2004 2475 7099 513 Size: 552 939 471 4624 3834 Fragments arranged by size: 4624 3834 939 552 471 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9681 Fragments arranged by size: 9681 683 56 BtsI GCAGTG_nn' Cuts at: 2641 3567 3603 3905 5544 6558 7936 8730 8750 Size: 926 36 302 1639 1014 1378 794 20 Cuts at: 8750 2641 Size: 4311 Fragments arranged by size: 4311 1639 1378 1014 926 794 302 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10420 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3662 3697 529 Size: 35 3098 35 7252 Fragments arranged by size: 7252 3098 35 35 DraI TTT'AAA Cuts at: 7724 7724 Size: 10420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4665 4703 9427 809 Size: 507 3349 38 4724 1802 Fragments arranged by size: 4724 3349 1802 507 38 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4028 4407 5064 Size: 54 1425 50 275 18 2198 379 657 Cuts at: 5064 5203 6824 7228 7276 7867 8756 10385 8 Size: 139 1621 404 48 591 889 1629 43 Fragments arranged by size: 2198 1629 1621 1425 889 657 591 404 379 275 139 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7867 1537 Size: 275 6055 4090 Fragments arranged by size: 6055 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 3576 3752 5163 5172 5212 5513 6133 Size: 1371 852 176 1411 9 40 301 620 Cuts at: 6133 8350 1353 Size: 2217 3423 Fragments arranged by size: 3423 2217 1411 1371 852 620 301 176 40 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3700 3809 9007 9381 9527 387 Size: 1064 138 2111 109 5198 374 146 1280 Fragments arranged by size: 5198 2111 1280 1064 374 146 138 109 EcoICRI GAG'CTC Cuts at: 4948 4948 Size: 10420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2187 2218 2661 2765 2921 3040 3192 Size: 393 555 31 443 104 156 119 152 Cuts at: 3192 3315 3339 3375 3399 3453 3612 3817 3823 Size: 123 24 36 24 54 159 205 6 Cuts at: 3823 4112 4328 4736 5012 5106 5651 5699 5705 Size: 289 216 408 276 94 545 48 6 Cuts at: 5705 5711 5768 6099 6198 6596 6866 7016 7656 Size: 6 57 331 99 398 270 150 640 Cuts at: 7656 8483 9080 9867 10135 1239 Size: 827 597 787 268 1524 Fragments arranged by size: 1524 827 787 640 597 555 545 443 408 398 393 331 289 276 270 268 216 205 159 156 152 150 123 119 104 99 94 57 54 48 36 31 24 24 6 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 2088 3123 4652 5354 2088 Size: 1035 1529 702 7154 Fragments arranged by size: 7154 1529 1035 702 EcoO109I rG'GnC_Cy Cuts at: 2098 2329 4645 6364 6853 7325 2098 Size: 231 2316 1719 489 472 5193 Fragments arranged by size: 5193 2316 1719 489 472 231 EcoRI G'AATT_C Cuts at: 7196 7729 8107 7196 Size: 533 378 9509 Fragments arranged by size: 9509 533 378 EcoRV GAT'ATC Cuts at: 6691 6903 6691 Size: 212 10208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3680 3680 Size: 10420 FspI TGC'GCA Cuts at: 3698 4880 8926 3698 Size: 1182 4046 5192 Fragments arranged by size: 5192 4046 1182 FspAI rTGC'GCAy Cuts at: 3698 3698 Size: 10420 HaeII r_GCGC'y Cuts at: 1678 4423 5269 6940 9567 1678 Size: 2745 846 1671 2627 2531 Fragments arranged by size: 2745 2627 2531 1671 846 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3236 3268 3914 3946 4769 Size: 32 135 32 1664 32 646 32 823 Cuts at: 4769 4799 4801 4831 4985 5017 5930 5962 6319 Size: 30 2 30 154 32 913 32 357 Cuts at: 6319 6351 6904 6936 7418 7450 9061 9093 9135 Size: 32 553 32 482 32 1611 32 42 Cuts at: 9135 9167 1373 Size: 32 2626 Fragments arranged by size: 2626 1664 1611 913 823 646 553 482 357 154 135 42 32 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3965 7027 7764 8010 669 Size: 988 281 1524 503 3062 737 246 3079 Fragments arranged by size: 3079 3062 1524 988 737 503 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10420 HpaI GTT'AAC Cuts at: 8010 8010 Size: 10420 KasI G'GCGC_C Cuts at: 6936 6936 Size: 10420 KpnI G_GTAC'C Cuts at: 2466 6808 7495 7745 2466 Size: 4342 687 250 5141 Fragments arranged by size: 5141 4342 687 250 MfeI C'AATT_G Cuts at: 8019 8019 Size: 10420 MluI A'CGCG_T Cuts at: 10296 10296 Size: 10420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3545 3697 3838 5562 6327 6699 9534 9718 Size: 2240 152 141 1724 765 372 2835 184 Cuts at: 9718 1305 Size: 2007 Fragments arranged by size: 2835 2240 2007 1724 765 372 184 152 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 4409 5066 6826 7230 10 Size: 54 1425 343 2577 657 1760 404 3200 Fragments arranged by size: 3200 2577 1760 1425 657 404 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2649 7392 8378 8737 8896 518 Size: 648 843 640 4743 986 359 159 2042 Fragments arranged by size: 4743 2042 986 843 648 640 359 159 MspA1I CmG'CkG Cuts at: 1606 3512 5195 6280 6458 6583 6994 7683 7836 Size: 1906 1683 1085 178 125 411 689 153 Cuts at: 7836 8513 9661 9906 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1906 1683 1148 1085 689 677 411 245 178 153 125 NaeI GCC'GGC Cuts at: 2396 5039 2396 Size: 2643 7777 NarI GG'CG_CC Cuts at: 6937 6937 Size: 10420 NcoI C'CATG_G Cuts at: 513 1065 2004 2475 513 Size: 552 939 471 8458 Fragments arranged by size: 8458 939 552 471 NdeI CA'TA_TG Cuts at: 387 4846 6907 387 Size: 4459 2061 3900 Fragments arranged by size: 4459 3900 2061 NgoMIV G'CCGG_C Cuts at: 2394 5037 2394 Size: 2643 7777 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10420 NotI GC'GGCC_GC Cuts at: 7867 7867 Size: 10420 NspI r_CATG'y Cuts at: 1132 1229 6624 7041 7778 9323 1132 Size: 97 5395 417 737 1545 2229 Fragments arranged by size: 5395 2229 1545 737 417 97 PasI CC'CwG_GG Cuts at: 1348 5350 1348 Size: 4002 6418 PciI A'CATG_T Cuts at: 6620 7037 9319 6620 Size: 417 2282 7721 Fragments arranged by size: 7721 2282 417 PflMI CCAn_nnn'nTGG Cuts at: 1108 2936 7011 7226 1108 Size: 1828 4075 215 4302 Fragments arranged by size: 4302 4075 1828 215 PfoI T'CCnGG_A Cuts at: 5745 5884 6384 5745 Size: 139 500 9781 Fragments arranged by size: 9781 500 139 PmeI GTTT'AAAC Cuts at: 7724 7724 Size: 10420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5116 5148 8489 8521 10002 10034 5116 Size: 32 3341 32 1481 32 5502 Fragments arranged by size: 5502 3341 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2329 4645 2329 Size: 2316 8104 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10420 PsiI TTA'TAA Cuts at: 7990 7990 Size: 10420 PspOMI G'GGCC_C Cuts at: 5565 5565 Size: 10420 PspXI vC'TCGA_Gb Cuts at: 6947 6947 Size: 10420 PstI C_TGCA'G Cuts at: 830 4653 6028 6606 7772 830 Size: 3823 1375 578 1166 3478 Fragments arranged by size: 3823 3478 1375 1166 578 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10420 PvuII CAG'CTG Cuts at: 3512 5195 6280 6458 6994 3512 Size: 1683 1085 178 536 6938 Fragments arranged by size: 6938 1683 1085 536 178 SacI G_AGCT'C Cuts at: 4950 4950 Size: 10420 SalI G'TCGA_C Cuts at: 1936 3963 7762 1936 Size: 2027 3799 4594 Fragments arranged by size: 4594 3799 2027 SapI GCTCTTCn'nnn_ Cuts at: 2724 2724 Size: 10420 SbfI CC_TGCA'GG Cuts at: 4653 7772 4653 Size: 3119 7301 ScaI AGT'ACT Cuts at: 8668 10380 8668 Size: 1712 8708 SexAI A'CCwGG_T Cuts at: 3734 3734 Size: 10420 SfcI C'TryA_G Cuts at: 826 1045 3378 4649 5008 5187 5365 6024 6602 Size: 219 2333 1271 359 179 178 659 578 Cuts at: 6602 6894 7768 8903 9584 9775 826 Size: 292 874 1135 681 191 1471 Fragments arranged by size: 2333 1471 1271 1135 874 681 659 578 359 292 219 191 179 178 SfoI GGC'GCC Cuts at: 6938 6938 Size: 10420 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10420 SmaI CCC'GGG Cuts at: 1872 5564 5596 7747 1872 Size: 3692 32 2151 4545 Fragments arranged by size: 4545 3692 2151 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 2996 5618 5961 6359 6947 Size: 197 926 381 672 2622 343 398 588 Cuts at: 6947 8524 9425 9687 820 Size: 1577 901 262 1553 Fragments arranged by size: 2622 1577 1553 926 901 672 588 398 381 343 262 197 SnaBI TAC'GTA Cuts at: 493 8211 493 Size: 7718 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10420 SphI G_CATG'C Cuts at: 1132 7778 1132 Size: 6646 3774 SrfI GCCC'GGGC Cuts at: 5564 5564 Size: 10420 SspI AAT'ATT Cuts at: 5 52 8344 5 Size: 47 8292 2081 Fragments arranged by size: 8292 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 10420 StyI C'CwwG_G Cuts at: 513 1065 2004 2383 2475 3124 4005 4195 4266 Size: 552 939 379 92 649 881 190 71 Cuts at: 4266 5609 6138 7859 513 Size: 1343 529 1721 3074 Fragments arranged by size: 3074 1721 1343 939 881 649 552 529 379 190 92 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2963 3669 3892 4673 4898 7080 8447 Size: 1255 1383 706 223 781 225 2182 1367 Cuts at: 8447 8464 8617 8802 9247 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2182 1498 1383 1367 1255 781 706 445 225 223 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7163 8666 10378 Size: 274 80 33 51 1443 5186 1503 1712 Cuts at: 10378 96 Size: 138 Fragments arranged by size: 5186 1712 1503 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10380 10412 10380 Size: 32 10388 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10420 XbaI T'CTAG_A Cuts at: 3559 7756 3559 Size: 4197 6223 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3574 7152 3574 Size: 3578 6842 XhoI C'TCGA_G Cuts at: 1943 6947 1943 Size: 5004 5416 XmaI C'CCGG_G Cuts at: 1870 5562 5594 7745 1870 Size: 3692 32 2151 4545 Fragments arranged by size: 4545 3692 2151 32 XmnI GAAnn'nnTTC Cuts at: 3657 8549 3657 Size: 4892 5528 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5372 8228 276 Size: 53 83 186 936 3838 2856 2468 Fragments arranged by size: 3838 2856 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BfrBI BsiWI BstZ17I DraIII FseI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI SwaI