Restriction map for
pFN21AE0092
(Linear) (Six-base) MAPSORT of: KIEE0092.seq Check: 7496 from: 1 to: 5715
KIEE0092.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 60 5169 5715
Size: 60 5109 546
Fragments arranged by size:
5109 546 60
AatII G_ACGT'C
Cuts at: 0 3369 5715
Size: 3369 2346
AccI GT'mk_AC
Cuts at: 0 1959 5715
Size: 1959 3756
Acc65I G'GTAC_C
Cuts at: 0 457 4799 5486 5715
Size: 457 4342 687 229
Fragments arranged by size:
4342 687 457 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 656 760 1035 1187 1448 1812 1818
Size: 182 474 104 275 152 261 364 6
Cuts at: 1818 2323 2731 3101 3646 3694 3700 3706 5651
Size: 505 408 370 545 48 6 6 1945
Cuts at: 5651 5715
Size: 64
Fragments arranged by size:
1945 545 505 474 408 370 364 275
261 182 152 104 64 48 6 6
6
AflII C'TTAA_G
Cuts at: 0 3613 5715
Size: 3613 2102
AflIII A'CryG_T
Cuts at: 0 4615 5032 5715
Size: 4615 417 683
Fragments arranged by size:
4615 683 417
AhdI GACnn_n'nnGTC
Cuts at: 0 2808 5715
Size: 2808 2907
AleI CACnn'nnGTG
Cuts at: 0 5387 5715
Size: 5387 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2930 3325 4499 5715
Size: 2930 395 1174 1216
Fragments arranged by size:
2930 1216 1174 395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3111 3143 5715
Size: 3111 32 2572
Fragments arranged by size:
3111 2572 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 1507 2502 3928 4027 5715
Size: 1237 270 995 1426 99 1688
Fragments arranged by size:
1688 1426 1237 995 270 99
ApaI G_GGCC'C
Cuts at: 0 3564 5715
Size: 3564 2151
ApoI r'AATT_y
Cuts at: 0 522 5191 5715
Size: 522 4669 524
Fragments arranged by size:
4669 524 522
AvaI C'yCGr_G
Cuts at: 0 348 3557 3563 3589 4370 4942 5715
Size: 348 3209 6 26 781 572 773
Fragments arranged by size:
3209 781 773 572 348 26 6
AvrII C'CTAG_G
Cuts at: 0 1119 3604 5715
Size: 1119 2485 2111
Fragments arranged by size:
2485 2111 1119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4783 4816 5715
Size: 4783 33 899
Fragments arranged by size:
4783 899 33
BanI G'GyrC_C
Cuts at: 0 457 915 4719 4799 4870 4882 4931 4949
Size: 457 458 3804 80 71 12 49 18
Cuts at: 4949 5486 5715
Size: 537 229
Fragments arranged by size:
3804 537 458 457 229 80 71 49
18 12
BanII G_rGCy'C
Cuts at: 0 902 2945 3564 4472 4814 5345 5715
Size: 902 2043 619 908 342 531 370
Fragments arranged by size:
2043 908 902 619 531 370 342
BbeI G_GCGC'C
Cuts at: 0 4935 5715
Size: 4935 780
BbsI GAAGACnn'nnnn_
Cuts at: 0 872 893 1386 1651 4531 4649 5715
Size: 872 21 493 265 2880 118 1066
Fragments arranged by size:
2880 1066 872 493 265 118 21
BbvCI CC'TCA_GC
Cuts at: 0 1419 3704 3790 4052 4449 4724 5715
Size: 1419 2285 86 262 397 275 991
Fragments arranged by size:
2285 1419 991 397 275 262 86
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 4316 4387 5715
Size: 646 3670 71 1328
Fragments arranged by size:
3670 1328 646 71
BclI T'GATC_A
Cuts at: 0 855 1239 1778 5715
Size: 855 384 539 3937
Fragments arranged by size:
3937 855 539 384
BglI GCCn_nnn'nGGC
Cuts at: 0 4818 5715
Size: 4818 897
BglII A'GATC_T
Cuts at: 0 2762 5715
Size: 2762 2953
BlpI GC'TnA_GC
Cuts at: 0 3674 3817 4855 5174 5715
Size: 3674 143 1038 319 541
Fragments arranged by size:
3674 1038 541 319 143
Bme1580I G_kGCm'C
Cuts at: 0 3564 4875 4885 5651 5715
Size: 3564 1311 10 766 64
Fragments arranged by size:
3564 1311 766 64 10
BmgBI CAC'GTC
Cuts at: 0 1572 5715
Size: 1572 4143
BmrI ACTGGGnnnn_n'
Cuts at: 0 336 2534 3080 3384 3778 4226 5715
Size: 336 2198 546 304 394 448 1489
Fragments arranged by size:
2198 1489 546 448 394 336 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1612 3842 4323 4683 5643 5715
Size: 1612 2230 481 360 960 72
Fragments arranged by size:
2230 1612 960 481 360 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 916 1310 1334 1370 1394 1607 2107
Size: 213 703 394 24 36 24 213 500
Cuts at: 2107 3007 3763 4094 4193 4591 4861 5011 5715
Size: 900 756 331 99 398 270 150 704
Fragments arranged by size:
900 756 704 703 500 398 394 331
270 213 213 150 99 36 24 24
Bpu10I CC'TnA_GC
Cuts at: 0 1419 1789 3704 3790 4052 4308 4449 4724
Size: 1419 370 1915 86 262 256 141 275
Cuts at: 4724 5542 5715
Size: 818 173
Fragments arranged by size:
1915 1419 818 370 275 262 256 173
141 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1012 3941 4339 5715
Size: 304 708 2929 398 1376
Fragments arranged by size:
2929 1376 708 398 304
BsaI GGTCTCn'nnnn_
Cuts at: 0 116 801 1410 1720 2215 3139 3345 4160
Size: 116 685 609 310 495 924 206 815
Cuts at: 4160 4218 5715
Size: 58 1497
Fragments arranged by size:
1497 924 815 685 609 495 310 206
116 58
BsaHI Gr'CG_yC
Cuts at: 0 3366 4932 5280 5715
Size: 3366 1566 348 435
Fragments arranged by size:
3366 1566 435 348
BsaWI w'CCGG_w
Cuts at: 0 3038 5715
Size: 3038 2677
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1717 1747 1909 1939 2764 2794 2980 3010
Size: 1717 30 162 30 825 30 186 30
Cuts at: 3010 3111 3141 5413 5443 5715
Size: 101 30 2272 30 272
Fragments arranged by size:
2272 1717 825 272 186 162 101 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 1641 1930 2125 2371 3157 3190 3451
Size: 216 1425 289 195 246 786 33 261
Cuts at: 3451 3931 4056 4579 5581 5698 5715
Size: 480 125 523 1002 117 17
Fragments arranged by size:
1425 1002 786 523 480 289 261 246
216 195 125 117 33 17
BseYI C'CCAG_C
Cuts at: 0 128 253 430 1226 1302 1431 1500 1527
Size: 128 125 177 796 76 129 69 27
Cuts at: 1527 2601 2879 2894 2903 3656 3692 3698 3894
Size: 1074 278 15 9 753 36 6 196
Cuts at: 3894 4138 4200 4271 4283 5310 5644 5678 5715
Size: 244 62 71 12 1027 334 34 37
Fragments arranged by size:
1074 1027 796 753 334 278 244 196
177 129 128 125 76 71 69 62
37 36 34 27 15 12 9 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 992 1921 2702 4135 5715
Size: 32 960 929 781 1433 1580
Fragments arranged by size:
1580 1433 960 929 781 32
BsiHKAI G_wGCw'C
Cuts at: 0 1826 2945 4309 4664 5715
Size: 1826 1119 1364 355 1051
Fragments arranged by size:
1826 1364 1119 1051 355
BsmI GAATG_Cn'
Cuts at: 0 596 3112 4919 5715
Size: 596 2516 1807 796
Fragments arranged by size:
2516 1807 796 596
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1623 5715
Size: 1623 4092
Bsp1286I G_dGCh'C
Cuts at: 0 902 1826 2945 3564 4309 4472 4664 4814
Size: 902 924 1119 619 745 163 192 150
Cuts at: 4814 4875 4885 5345 5651 5715
Size: 61 10 460 306 64
Fragments arranged by size:
1119 924 902 745 619 460 306 192
163 150 64 61 10
BspEI T'CCGG_A
Cuts at: 0 3038 5715
Size: 3038 2677
BspHI T'CATG_A
Cuts at: 0 1622 4827 5715
Size: 1622 3205 888
Fragments arranged by size:
3205 1622 888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 2702 4491 4811 5169 5715
Size: 60 2642 1789 320 358 546
Fragments arranged by size:
2642 1789 546 358 320 60
BsrBI CCG'CTC
Cuts at: 0 4947 5535 5715
Size: 4947 588 180
Fragments arranged by size:
4947 588 180
BsrDI GCAATG_nn'
Cuts at: 0 2533 4002 5000 5715
Size: 2533 1469 998 715
Fragments arranged by size:
2533 1469 998 715
BsrFI r'CCGG_y
Cuts at: 0 389 3032 5489 5715
Size: 389 2643 2457 226
Fragments arranged by size:
2643 2457 389 226
BssSI C'ACGA_G
Cuts at: 0 2516 5715
Size: 2516 3199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 55 1899 2128 2694 3481 5655 5715
Size: 55 1844 229 566 787 2174 60
Fragments arranged by size:
2174 1844 787 566 229 60 55
BstEII G'GTnAC_C
Cuts at: 0 2361 5715
Size: 2361 3354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2731 3156 4134 4200 4746 5715
Size: 2731 425 978 66 546 969
Fragments arranged by size:
2731 978 969 546 425 66
BstYI r'GATC_y
Cuts at: 0 589 1919 2762 2786 2816 4329 5715
Size: 589 1330 843 24 30 1513 1386
Fragments arranged by size:
1513 1386 1330 843 589 30 24
Bsu36I CC'TnA_GG
Cuts at: 0 60 84 2030 3434 3668 4877 5715
Size: 60 24 1946 1404 234 1209 838
Fragments arranged by size:
1946 1404 1209 838 234 60 24
BtgI C'CryG_G
Cuts at: 0 470 5094 5715
Size: 470 4624 621
Fragments arranged by size:
4624 621 470
BtsI GCAGTG_nn'
Cuts at: 0 636 1562 1598 1900 3539 4553 5715
Size: 636 926 36 302 1639 1014 1162
Fragments arranged by size:
1639 1162 1014 926 636 302 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1657 1692 5715
Size: 1657 35 4023
Fragments arranged by size:
4023 1657 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2660 2698 5715
Size: 2660 38 3017
Fragments arranged by size:
3017 2660 38
EaeI y'GGCC_r
Cuts at: 0 2023 2402 3059 3198 4819 5223 5271 5715
Size: 2023 379 657 139 1621 404 48 444
Fragments arranged by size:
2023 1621 657 444 404 379 139 48
EarI CTCTTCn'nnn_
Cuts at: 0 719 1571 1747 3158 3167 3207 3508 4128
Size: 719 852 176 1411 9 40 301 620
Cuts at: 4128 5715
Size: 1587
Fragments arranged by size:
1587 1411 852 719 620 301 176 40
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1695 1804 5715
Size: 1695 109 3911
Fragments arranged by size:
3911 1695 109
EcoICRI GAG'CTC
Cuts at: 0 2943 5715
Size: 2943 2772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 213 656 760 916 1035 1187 1310
Size: 182 31 443 104 156 119 152 123
Cuts at: 1310 1334 1370 1394 1448 1607 1812 1818 2107
Size: 24 36 24 54 159 205 6 289
Cuts at: 2107 2323 2731 3007 3101 3646 3694 3700 3706
Size: 216 408 276 94 545 48 6 6
Cuts at: 3706 3763 4094 4193 4591 4861 5011 5651 5715
Size: 57 331 99 398 270 150 640 64
Fragments arranged by size:
640 545 443 408 398 331 289 276
270 216 205 182 159 156 152 150
123 119 104 99 94 64 57 54
48 36 31 24 24 6 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 83 1118 2647 3349 5715
Size: 83 1035 1529 702 2366
Fragments arranged by size:
2366 1529 1035 702 83
EcoO109I rG'GnC_Cy
Cuts at: 0 93 324 2640 4359 4848 5320 5715
Size: 93 231 2316 1719 489 472 395
Fragments arranged by size:
2316 1719 489 472 395 231 93
EcoRI G'AATT_C
Cuts at: 0 5191 5715
Size: 5191 524
EcoRV GAT'ATC
Cuts at: 0 4686 4898 5715
Size: 4686 212 817
Fragments arranged by size:
4686 817 212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1675 5715
Size: 1675 4040
FspI TGC'GCA
Cuts at: 0 1693 2875 5715
Size: 1693 1182 2840
Fragments arranged by size:
2840 1693 1182
FspAI rTGC'GCAy
Cuts at: 0 1693 5715
Size: 1693 4022
HaeII r_GCGC'y
Cuts at: 0 2418 3264 4935 5715
Size: 2418 846 1671 780
Fragments arranged by size:
2418 1671 846 780
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1909 1941 2764 2794 2796 2826
Size: 1231 32 646 32 823 30 2 30
Cuts at: 2826 2980 3012 3925 3957 4314 4346 4899 4931
Size: 154 32 913 32 357 32 553 32
Cuts at: 4931 5413 5445 5715
Size: 482 32 270
Fragments arranged by size:
1231 913 823 646 553 482 357 270
154 32 32 32 32 32 32 32
30 30 2
HincII GTy'rAC
Cuts at: 0 1457 1960 5022 5715
Size: 1457 503 3062 693
Fragments arranged by size:
3062 1457 693 503
KasI G'GCGC_C
Cuts at: 0 4931 5715
Size: 4931 784
KpnI G_GTAC'C
Cuts at: 0 461 4803 5490 5715
Size: 461 4342 687 225
Fragments arranged by size:
4342 687 461 225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1540 1692 1833 3557 4322 4694 5715
Size: 1540 152 141 1724 765 372 1021
Fragments arranged by size:
1724 1540 1021 765 372 152 141
MscI TGG'CCA
Cuts at: 0 2404 3061 4821 5225 5715
Size: 2404 657 1760 404 490
Fragments arranged by size:
2404 1760 657 490 404
MslI CAynn'nnrTG
Cuts at: 0 644 5387 5715
Size: 644 4743 328
Fragments arranged by size:
4743 644 328
MspA1I CmG'CkG
Cuts at: 0 1507 3190 4275 4453 4578 4989 5678 5715
Size: 1507 1683 1085 178 125 411 689 37
Fragments arranged by size:
1683 1507 1085 689 411 178 125 37
NaeI GCC'GGC
Cuts at: 0 391 3034 5715
Size: 391 2643 2681
Fragments arranged by size:
2681 2643 391
NarI GG'CG_CC
Cuts at: 0 4932 5715
Size: 4932 783
NcoI C'CATG_G
Cuts at: 0 470 5715
Size: 470 5245
NdeI CA'TA_TG
Cuts at: 0 2841 4902 5715
Size: 2841 2061 813
Fragments arranged by size:
2841 2061 813
NgoMIV G'CCGG_C
Cuts at: 0 389 3032 5715
Size: 389 2643 2683
Fragments arranged by size:
2683 2643 389
NspI r_CATG'y
Cuts at: 0 4619 5036 5715
Size: 4619 417 679
Fragments arranged by size:
4619 679 417
PasI CC'CwG_GG
Cuts at: 0 3345 5715
Size: 3345 2370
PciI A'CATG_T
Cuts at: 0 4615 5032 5715
Size: 4615 417 683
Fragments arranged by size:
4615 683 417
PflMI CCAn_nnn'nTGG
Cuts at: 0 931 5006 5221 5715
Size: 931 4075 215 494
Fragments arranged by size:
4075 931 494 215
PfoI T'CCnGG_A
Cuts at: 0 3740 3879 4379 5715
Size: 3740 139 500 1336
Fragments arranged by size:
3740 1336 500 139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3111 3143 5715
Size: 3111 32 2572
Fragments arranged by size:
3111 2572 32
PpuMI rG'GwC_Cy
Cuts at: 0 324 2640 5715
Size: 324 2316 3075
Fragments arranged by size:
3075 2316 324
PspOMI G'GGCC_C
Cuts at: 0 3560 5715
Size: 3560 2155
PspXI vC'TCGA_Gb
Cuts at: 0 4942 5715
Size: 4942 773
PstI C_TGCA'G
Cuts at: 0 2648 4023 4601 5715
Size: 2648 1375 578 1114
Fragments arranged by size:
2648 1375 1114 578
PvuII CAG'CTG
Cuts at: 0 1507 3190 4275 4453 4989 5715
Size: 1507 1683 1085 178 536 726
Fragments arranged by size:
1683 1507 1085 726 536 178
SacI G_AGCT'C
Cuts at: 0 2945 5715
Size: 2945 2770
SalI G'TCGA_C
Cuts at: 0 1958 5715
Size: 1958 3757
SapI GCTCTTCn'nnn_
Cuts at: 0 719 5715
Size: 719 4996
SbfI CC_TGCA'GG
Cuts at: 0 2648 5715
Size: 2648 3067
SexAI A'CCwGG_T
Cuts at: 0 1729 5715
Size: 1729 3986
SfcI C'TryA_G
Cuts at: 0 1373 2644 3003 3182 3360 4019 4597 4889
Size: 1373 1271 359 179 178 659 578 292
Cuts at: 4889 5715
Size: 826
Fragments arranged by size:
1373 1271 826 659 578 359 292 179
178
SfoI GGC'GCC
Cuts at: 0 4933 5715
Size: 4933 782
SmaI CCC'GGG
Cuts at: 0 3559 3591 5715
Size: 3559 32 2124
Fragments arranged by size:
3559 2124 32
SmlI C'TyrA_G
Cuts at: 0 319 991 3613 3956 4354 4942 5715
Size: 319 672 2622 343 398 588 773
Fragments arranged by size:
2622 773 672 588 398 343 319
SrfI GCCC'GGGC
Cuts at: 0 3559 5715
Size: 3559 2156
StyI C'CwwG_G
Cuts at: 0 378 470 1119 2000 2190 2261 3604 4133
Size: 378 92 649 881 190 71 1343 529
Cuts at: 4133 5715
Size: 1582
Fragments arranged by size:
1582 1343 881 649 529 378 190 92
71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 958 1664 1887 2668 2893 5075 5715
Size: 958 706 223 781 225 2182 640
Fragments arranged by size:
2182 958 781 706 640 225 223
TatI w'GTAC_w
Cuts at: 0 5158 5715
Size: 5158 557
XbaI T'CTAG_A
Cuts at: 0 1554 5715
Size: 1554 4161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1569 5147 5715
Size: 1569 3578 568
Fragments arranged by size:
3578 1569 568
XhoI C'TCGA_G
Cuts at: 0 4942 5715
Size: 4942 773
XmaI C'CCGG_G
Cuts at: 0 3557 3589 5715
Size: 3557 32 2126
Fragments arranged by size:
3557 2126 32
XmnI GAAnn'nnTTC
Cuts at: 0 1652 5715
Size: 1652 4063
ZraI GAC'GTC
Cuts at: 0 3367 5715
Size: 3367 2348
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI AvrII
BaeI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SalI SapI SbfI SexAI SfcI
SfoI SmaI SmlI SrfI StyI TaqII TatI XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI ApaLI AscI AseI AsiSI BamHI
BcgI BfrBI BmtI BsaAI BsaBI BsiEI BsiWI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI DraI DraIII EagI
FseI HindIII HpaI MfeI MluI NheI NotI NruI
NsiI PacI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SacII SanDI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TstI Tth111I