(Circular) (Six-base) MAPSORT of: pFN21AE0091.seq Check: 522 from: 1 to: 6601 pFN21AE0091 6601 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4411 278 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 2766 3944 1937 Size: 813 16 1178 4594 Fragments arranged by size: 4594 1178 813 16 Acc65I G'GTAC_C Cuts at: 3097 3922 3097 Size: 825 5776 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4664 6048 6316 1632 Size: 3032 1384 268 1917 Fragments arranged by size: 3032 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 2220 2363 3276 5500 6477 1203 Size: 1017 143 913 2224 977 1327 Fragments arranged by size: 2224 1327 1017 977 913 143 AgeI A'CCGG_T Cuts at: 3486 5432 3486 Size: 1946 4655 AhdI GACnn_n'nnGTC Cuts at: 5330 5330 Size: 6601 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6601 AlwNI CAG_nnn'CTG Cuts at: 2560 3003 4344 5916 2560 Size: 443 1341 1572 3245 Fragments arranged by size: 3245 1572 1341 443 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2304 3910 4136 4288 6397 1493 Size: 811 1606 226 152 2109 1697 Fragments arranged by size: 2109 1697 1606 811 226 152 AseI AT'TA_AT Cuts at: 160 1032 5155 160 Size: 872 4123 1606 Fragments arranged by size: 4123 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 2831 3746 3926 1870 Size: 73 888 915 180 4545 Fragments arranged by size: 4545 915 888 180 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2713 2746 2713 Size: 33 6568 BamHI G'GATC_C Cuts at: 3931 4298 3931 Size: 367 6234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2104 3032 3097 3922 Size: 325 217 627 284 33 928 65 825 Cuts at: 3922 5377 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 928 825 627 325 284 217 65 33 BanII G_rGCy'C Cuts at: 1391 3815 3920 1391 Size: 2424 105 4072 Fragments arranged by size: 4072 2424 105 BbeI G_GCGC'C Cuts at: 2075 2108 2075 Size: 33 6568 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3513 3734 928 Size: 971 1614 221 3795 Fragments arranged by size: 3795 1614 971 221 BbvCI CC'TCA_GC Cuts at: 2049 3108 2049 Size: 1059 5542 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3022 3056 3445 3479 4792 4826 1903 Size: 34 1085 34 389 34 1313 34 3678 Fragments arranged by size: 3678 1313 1085 389 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4493 5703 4493 Size: 1210 5391 BclI T'GATC_A Cuts at: 1548 3555 3600 1548 Size: 2007 45 4549 Fragments arranged by size: 4549 2007 45 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5212 136 Size: 107 122 71 631 605 337 3203 1525 Fragments arranged by size: 3203 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2919 3781 6596 2919 Size: 862 2815 2924 Fragments arranged by size: 2924 2815 862 BlpI GC'TnA_GC Cuts at: 2186 4018 2186 Size: 1832 4769 Bme1580I G_kGCm'C Cuts at: 1790 4662 5818 1790 Size: 2872 1156 2573 Fragments arranged by size: 2872 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6601 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4332 5290 442 Size: 416 53 83 399 24 2915 958 1753 Fragments arranged by size: 2915 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 3740 5261 1239 Size: 1103 1398 1521 2579 Fragments arranged by size: 2579 1521 1398 1103 Bpu10I CC'TnA_GC Cuts at: 2049 2477 3108 2049 Size: 428 631 5542 Fragments arranged by size: 5542 631 428 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3602 3836 4726 5591 5889 3602 Size: 234 890 865 298 4314 Fragments arranged by size: 4314 890 865 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5264 882 Size: 617 3765 2219 Fragments arranged by size: 3765 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2281 2364 2770 4392 493 Size: 711 1077 83 406 1622 2702 Fragments arranged by size: 2702 1622 1077 711 406 83 BsaBI GATnn'nnATC Cuts at: 3212 3587 3713 6595 3212 Size: 375 126 2882 3218 Fragments arranged by size: 3218 2882 375 126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2072 2105 4408 4790 Size: 53 83 186 936 539 33 2303 382 Cuts at: 4790 5425 275 Size: 635 1451 Fragments arranged by size: 2303 1451 936 635 539 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 3294 3486 5034 5432 5706 5853 6264 3294 Size: 192 1548 398 274 147 411 3631 Fragments arranged by size: 3631 1548 411 398 274 192 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6376 Fragments arranged by size: 6376 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2271 1186 Size: 880 205 5516 Fragments arranged by size: 5516 880 205 BseYI C'CCAG_C Cuts at: 2212 2679 3047 3468 3643 3773 5804 6300 2212 Size: 467 368 421 175 130 2031 496 2513 Fragments arranged by size: 2513 2031 496 467 421 368 175 130 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6028 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4051 4812 5840 1540 Size: 275 186 2050 761 1028 2301 Fragments arranged by size: 2301 2050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2631 2664 4662 4747 5818 2631 Size: 33 1998 85 1071 3414 Fragments arranged by size: 3414 1998 1071 85 33 BsiWI C'GTAC_G Cuts at: 3084 3084 Size: 6601 BsmI GAATG_Cn' Cuts at: 2264 2602 4112 4205 2264 Size: 338 1510 93 4660 Fragments arranged by size: 4660 1510 338 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3884 3884 Size: 6601 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2631 2664 3815 3920 4662 4747 5818 Size: 399 841 33 1151 105 742 85 1071 Cuts at: 5818 1391 Size: 2174 Fragments arranged by size: 2174 1151 1071 841 742 399 105 85 33 BspEI T'CCGG_A Cuts at: 3294 6264 3294 Size: 2970 3631 BspHI T'CATG_A Cuts at: 3204 3558 4490 6220 3204 Size: 354 932 1730 3585 Fragments arranged by size: 3585 1730 932 354 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3956 844 Size: 3112 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2960 3703 4488 1394 Size: 429 1137 743 785 3507 Fragments arranged by size: 3507 1137 785 743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2041 3102 5096 5270 65 Size: 1183 793 1061 1994 174 1396 Fragments arranged by size: 1994 1396 1183 1061 793 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3306 3486 3522 5245 5432 1700 Size: 110 1496 180 36 1723 187 2869 Fragments arranged by size: 2869 1723 1496 187 180 110 36 BsrGI T'GTAC_A Cuts at: 96 3384 96 Size: 3288 3313 BssHII G'CGCG_C Cuts at: 1925 2111 1925 Size: 186 6415 BssSI C'ACGA_G Cuts at: 2147 3359 4661 5673 2147 Size: 1212 1302 1012 3075 Fragments arranged by size: 3075 1302 1212 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6601 BstBI TT'CG_AA Cuts at: 5405 5405 Size: 6601 BstEII G'GTnAC_C Cuts at: 1184 3160 3259 3302 1184 Size: 1976 99 43 4483 Fragments arranged by size: 4483 1976 99 43 BstXI CCAn_nnnn'nTGG Cuts at: 2944 3043 2944 Size: 99 6502 BstYI r'GATC_y Cuts at: 1972 2919 3570 3582 3708 3781 3931 4298 4683 Size: 947 651 12 126 73 150 367 385 Cuts at: 4683 4700 6152 6238 6250 6596 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 947 651 385 367 346 150 126 86 73 17 12 12 BstZ17I GTA'TAC Cuts at: 2767 2767 Size: 6601 BtgI C'CryG_G Cuts at: 513 1065 3267 3815 513 Size: 552 2202 548 3299 Fragments arranged by size: 3299 2202 552 548 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3853 487 Size: 683 56 2627 3235 Fragments arranged by size: 3235 2627 683 56 BtsI GCAGTG_nn' Cuts at: 3740 4117 4911 4931 3740 Size: 377 794 20 5410 Fragments arranged by size: 5410 794 377 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6566 DraI TTT'AAA Cuts at: 3905 3905 Size: 6601 DraIII CAC_nnn'GTG Cuts at: 3723 3723 Size: 6601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2141 2894 5608 809 Size: 507 825 753 2714 1802 Fragments arranged by size: 2714 1802 825 753 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2193 2640 Size: 54 1425 50 275 18 325 38 447 Cuts at: 2640 3441 3524 4048 4937 6566 8 Size: 801 83 524 889 1629 43 Fragments arranged by size: 1629 1425 889 801 524 447 325 275 83 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 4048 1537 Size: 275 2236 4090 Fragments arranged by size: 4090 2236 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3440 4531 1353 Size: 2087 1091 3423 Fragments arranged by size: 3423 2087 1091 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 5188 5562 5708 387 Size: 1064 138 438 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 3740 4664 5261 6048 6316 1239 Size: 393 710 1398 924 597 787 268 1524 Fragments arranged by size: 1524 1398 924 787 710 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2635 2835 3125 2635 Size: 200 290 6111 Fragments arranged by size: 6111 290 200 EcoRI G'AATT_C Cuts at: 2304 3910 4288 2304 Size: 1606 378 4617 Fragments arranged by size: 4617 1606 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2875 2875 Size: 6601 FspI TGC'GCA Cuts at: 5107 5107 Size: 6601 HaeII r_GCGC'y Cuts at: 1678 2024 2075 2108 2230 5748 1678 Size: 346 51 33 122 3518 2531 Fragments arranged by size: 3518 2531 346 122 51 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5242 5274 5316 5348 1373 Size: 32 135 32 3670 32 42 32 2626 Fragments arranged by size: 3670 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3765 3945 4191 669 Size: 988 281 1431 396 180 246 3079 Fragments arranged by size: 3079 1431 988 396 281 246 180 HindIII A'AGCT_T Cuts at: 748 3800 748 Size: 3052 3549 HpaI GTT'AAC Cuts at: 3369 4191 3369 Size: 822 5779 KasI G'GCGC_C Cuts at: 2071 2104 2071 Size: 33 6568 KpnI G_GTAC'C Cuts at: 3101 3926 3101 Size: 825 5776 MfeI C'AATT_G Cuts at: 4200 4200 Size: 6601 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2348 2910 5715 5899 1305 Size: 1043 562 2805 184 2007 Fragments arranged by size: 2805 2007 1043 562 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2642 3443 10 Size: 54 1425 343 810 801 3168 Fragments arranged by size: 3168 1425 810 801 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 3167 3719 4559 4918 5077 518 Size: 648 1267 734 552 840 359 159 2042 Fragments arranged by size: 2042 1267 840 734 648 552 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 3030 3184 4017 4694 5842 6087 1606 Size: 488 936 154 833 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 833 677 488 245 154 NarI GG'CG_CC Cuts at: 2072 2105 2072 Size: 33 6568 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6049 NdeI CA'TA_TG Cuts at: 387 387 Size: 6601 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6601 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 3280 3385 3959 5504 1132 Size: 97 2051 105 574 1545 2229 Fragments arranged by size: 2229 2051 1545 574 105 97 PasI CC'CwG_GG Cuts at: 1348 2029 2505 3566 1348 Size: 681 476 1061 4383 Fragments arranged by size: 4383 1061 681 476 PciI A'CATG_T Cuts at: 3276 5500 3276 Size: 2224 4377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6601 PfoI T'CCnGG_A Cuts at: 2319 2319 Size: 6601 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4670 4702 6183 6215 4670 Size: 32 1481 32 5056 Fragments arranged by size: 5056 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2635 2635 Size: 6601 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6601 PsiI TTA'TAA Cuts at: 2973 2982 4171 2973 Size: 9 1189 5403 Fragments arranged by size: 5403 1189 9 PstI C_TGCA'G Cuts at: 830 2090 2595 3953 830 Size: 1260 505 1358 3478 Fragments arranged by size: 3478 1358 1260 505 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 SalI G'TCGA_C Cuts at: 1936 3943 1936 Size: 2007 4594 SbfI CC_TGCA'GG Cuts at: 3953 3953 Size: 6601 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SfcI C'TryA_G Cuts at: 826 1045 2086 2575 2591 2751 2995 3949 5084 Size: 219 1041 489 16 160 244 954 1135 Cuts at: 5084 5765 5956 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1041 954 681 489 244 219 191 160 16 SfoI GGC'GCC Cuts at: 2073 2106 2073 Size: 33 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6601 SmaI CCC'GGG Cuts at: 1872 2833 3928 1872 Size: 961 1095 4545 Fragments arranged by size: 4545 1095 961 SmlI C'TyrA_G Cuts at: 820 1017 1943 3617 3851 4705 5606 5868 820 Size: 197 926 1674 234 854 901 262 1553 Fragments arranged by size: 1674 1553 926 901 854 262 234 197 SnaBI TAC'GTA Cuts at: 493 4392 493 Size: 3899 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 3959 1132 Size: 2827 3774 SspI AAT'ATT Cuts at: 5 52 4525 5 Size: 47 4473 2081 Fragments arranged by size: 4473 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6601 StyI C'CwwG_G Cuts at: 513 1065 3153 3285 4040 513 Size: 552 2088 132 755 3074 Fragments arranged by size: 3074 2088 755 552 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3460 4628 4645 4798 4983 5428 325 Size: 1255 1880 1168 17 153 185 445 1498 Fragments arranged by size: 1880 1498 1255 1168 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 3384 3606 Size: 274 80 33 51 1443 462 945 222 Cuts at: 3606 3621 4847 6559 96 Size: 15 1226 1712 138 Fragments arranged by size: 1712 1443 1226 945 462 274 222 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6561 6593 6561 Size: 32 6569 Tth111I GACn'n_nGTC Cuts at: 1316 2746 1316 Size: 1430 5171 XbaI T'CTAG_A Cuts at: 3937 3937 Size: 6601 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3109 3109 Size: 6601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6601 XmaI C'CCGG_G Cuts at: 1870 2831 3926 1870 Size: 961 1095 4545 Fragments arranged by size: 4545 1095 961 XmnI GAAnn'nnTTC Cuts at: 4730 4730 Size: 6601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4409 276 Size: 53 83 186 936 2875 2468 Fragments arranged by size: 2875 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI