(Circular) (Six-base) MAPSORT of: pFN21AE0090.seq Check: 1535 from: 1 to: 6154 pFN21AE0090 6154 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3198 3497 1937 Size: 1261 299 4594 Fragments arranged by size: 4594 1261 299 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3321 4217 5601 5869 1632 Size: 1689 896 1384 268 1917 Fragments arranged by size: 1917 1689 1384 896 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 5053 6030 1203 Size: 3850 977 1327 Fragments arranged by size: 3850 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3204 1184 Size: 2020 4134 AlwNI CAG_nnn'CTG Cuts at: 2103 3897 5469 2103 Size: 1794 1572 2788 Fragments arranged by size: 2788 1794 1572 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 2684 2994 3308 3463 3689 3841 5950 1493 Size: 1191 310 314 155 226 152 2109 1697 Fragments arranged by size: 2109 1697 1191 314 310 226 155 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2559 3479 1870 Size: 73 616 920 4545 Fragments arranged by size: 4545 920 616 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2117 2150 2720 2753 2117 Size: 33 570 33 5518 Fragments arranged by size: 5518 570 33 33 BamHI G'GATC_C Cuts at: 2799 3484 3851 2799 Size: 685 367 5102 Fragments arranged by size: 5102 685 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3475 4930 618 Size: 325 217 627 1688 1455 1842 Fragments arranged by size: 1842 1688 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2940 3473 1391 Size: 1549 533 4072 Fragments arranged by size: 4072 1549 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 3224 928 Size: 971 130 1195 3858 Fragments arranged by size: 3858 1195 971 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 2286 1548 Size: 738 5416 BfrBI ATG'CAT Cuts at: 2579 2579 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2444 4765 136 Size: 107 122 71 631 605 772 2321 1525 Fragments arranged by size: 2321 1525 772 631 605 122 107 71 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 3571 3571 Size: 6154 Bme1580I G_kGCm'C Cuts at: 1790 3077 4215 5371 1790 Size: 1287 1138 1156 2573 Fragments arranged by size: 2573 1287 1156 1138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3885 4843 442 Size: 416 53 83 399 24 2468 958 1753 Fragments arranged by size: 2468 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2470 2610 2881 2986 4814 1239 Size: 886 345 140 271 105 1828 2579 Fragments arranged by size: 2579 1828 886 345 271 140 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4817 882 Size: 617 3318 2219 Fragments arranged by size: 3318 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3175 3945 493 Size: 711 1971 770 2702 Fragments arranged by size: 2702 1971 770 711 BsaBI GATnn'nnATC Cuts at: 2541 6148 2541 Size: 3607 2547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2955 4587 4985 5259 5406 5817 2955 Size: 1632 398 274 147 411 3292 Fragments arranged by size: 3292 1632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5929 Fragments arranged by size: 5929 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2619 3129 1186 Size: 1433 510 4211 Fragments arranged by size: 4211 1433 510 BseYI C'CCAG_C Cuts at: 2926 3002 3027 5357 5853 2926 Size: 76 25 2330 496 3227 Fragments arranged by size: 3227 2330 496 76 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3197 1163 Size: 573 1461 4120 Fragments arranged by size: 4120 1461 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2940 4215 4300 5371 2016 Size: 924 1275 85 1071 2799 Fragments arranged by size: 2799 1275 1071 924 85 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 BsmBI CGTCTCn'nnnn_ Cuts at: 2962 2962 Size: 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2940 3077 3473 4215 4300 5371 Size: 399 226 924 137 396 742 85 1071 Cuts at: 5371 1391 Size: 2174 Fragments arranged by size: 2174 1071 924 742 399 396 226 137 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 2680 4043 5773 2680 Size: 1363 1730 3061 Fragments arranged by size: 3061 1730 1363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2965 3509 844 Size: 2121 544 3489 Fragments arranged by size: 3489 2121 544 BsrBI CCG'CTC Cuts at: 1394 1823 4041 1394 Size: 429 2218 3507 Fragments arranged by size: 3507 2218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4649 4823 65 Size: 1183 3401 174 1396 Fragments arranged by size: 3401 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstYI r'GATC_y Cuts at: 1972 2799 3484 3851 4236 4253 5705 5791 5803 Size: 827 685 367 385 17 1452 86 12 Cuts at: 5803 6149 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 827 685 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3203 3203 Size: 6154 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5602 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2433 3075 3670 4464 4484 2064 Size: 369 642 595 794 20 3734 Fragments arranged by size: 3734 794 642 595 369 20 ClaI AT'CG_AT Cuts at: 1554 3333 1554 Size: 1779 4375 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2689 3325 Size: 54 1425 50 275 18 489 370 636 Cuts at: 3325 3601 4490 6119 8 Size: 276 889 1629 43 Fragments arranged by size: 1629 1425 889 636 489 370 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2951 4084 1353 Size: 986 612 1133 3423 Fragments arranged by size: 3423 1133 986 612 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2938 2938 Size: 6154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2470 2610 2881 2986 3321 4217 Size: 393 493 345 140 271 105 335 896 Cuts at: 4217 4814 5601 5869 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 896 787 597 493 393 345 335 271 268 140 105 EcoNI CCTnn'n_nnAGG Cuts at: 2095 2126 2592 2095 Size: 31 466 5657 Fragments arranged by size: 5657 466 31 EcoO109I rG'GnC_Cy Cuts at: 2100 3095 3134 2100 Size: 995 39 5120 Fragments arranged by size: 5120 995 39 EcoRI G'AATT_C Cuts at: 2994 3463 3841 2994 Size: 469 378 5307 Fragments arranged by size: 5307 469 378 EcoRV GAT'ATC Cuts at: 2511 2511 Size: 6154 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 2460 5301 1678 Size: 782 2841 2531 Fragments arranged by size: 2841 2531 782 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2760 2792 2885 2917 4795 Size: 32 135 32 1188 32 93 32 1878 Cuts at: 4795 4827 4869 4901 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1878 1188 135 93 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3498 3744 669 Size: 988 281 1560 246 3079 Fragments arranged by size: 3079 1560 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5268 5452 1305 Size: 3963 184 2007 Fragments arranged by size: 3963 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 2691 3327 10 Size: 54 1425 343 489 370 636 2837 Fragments arranged by size: 2837 1425 636 489 370 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4112 4471 4630 518 Size: 648 843 2103 359 159 2042 Fragments arranged by size: 2103 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3570 4247 5395 5640 1606 Size: 1964 677 1148 245 2120 Fragments arranged by size: 2120 1964 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5602 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NsiI A_TGCA'T Cuts at: 2581 2581 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 3512 5057 1132 Size: 97 2283 1545 2229 Fragments arranged by size: 2283 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2698 1348 Size: 1350 4804 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 2103 2552 2698 1108 Size: 995 449 146 4564 Fragments arranged by size: 4564 995 449 146 PfoI T'CCnGG_A Cuts at: 2587 3440 2587 Size: 853 5301 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PmlI CAC'GTG Cuts at: 3175 3175 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 3095 3134 2100 Size: 995 39 5120 Fragments arranged by size: 5120 995 39 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 2748 3724 2748 Size: 976 5178 PstI C_TGCA'G Cuts at: 830 2071 3506 830 Size: 1241 1435 3478 Fragments arranged by size: 3478 1435 1241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 SacI G_AGCT'C Cuts at: 2940 2940 Size: 6154 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SapI GCTCTTCn'nnn_ Cuts at: 2951 2951 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 4402 6114 4402 Size: 1712 4442 SfcI C'TryA_G Cuts at: 826 1045 2067 3247 3342 3502 4637 5318 5509 Size: 219 1022 1180 95 160 1135 681 191 Cuts at: 5509 826 Size: 1471 Fragments arranged by size: 1471 1180 1135 1022 681 219 191 160 95 SfiI GGCCn_nnn'nGGCC Cuts at: 2444 2444 Size: 6154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4258 5159 5421 820 Size: 197 926 2315 901 262 1553 Fragments arranged by size: 2315 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 2147 4078 5 Size: 47 2095 1931 2081 Fragments arranged by size: 2095 2081 1931 47 StuI AGG'CCT Cuts at: 1517 2447 2598 1517 Size: 930 151 5073 Fragments arranged by size: 5073 930 151 StyI C'CwwG_G Cuts at: 513 1065 3167 3593 513 Size: 552 2102 426 3074 Fragments arranged by size: 3074 2102 552 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4181 4198 4351 4536 4981 325 Size: 1255 2601 17 153 185 445 1498 Fragments arranged by size: 2601 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2751 4400 6112 Size: 274 80 33 51 1443 774 1649 1712 Cuts at: 6112 96 Size: 138 Fragments arranged by size: 1712 1649 1443 774 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2522 2554 2858 2890 6114 6146 2522 Size: 32 304 32 3224 32 2530 Fragments arranged by size: 3224 2530 304 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 2200 2828 4283 2200 Size: 628 1455 4071 Fragments arranged by size: 4071 1455 628 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI