(Circular) (Six-base) MAPSORT of: pFN21AE0087.seq Check: 3314 from: 1 to: 7471 pFN21AE0087 7471 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5281 278 Size: 53 83 186 936 3745 2468 Fragments arranged by size: 3745 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4814 1937 Size: 2877 4594 Acc65I G'GTAC_C Cuts at: 4792 4792 Size: 7471 AclI AA'CG_TT Cuts at: 1563 5598 5971 1563 Size: 4035 373 3063 Fragments arranged by size: 4035 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2408 4452 5534 6918 7186 1632 Size: 618 158 2044 1082 1384 268 1917 Fragments arranged by size: 2044 1917 1384 1082 618 268 158 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7274 AflIII A'CryG_T Cuts at: 1203 2127 3708 4076 6370 7347 1203 Size: 924 1581 368 2294 977 1327 Fragments arranged by size: 2294 1581 1327 977 924 368 AgeI A'CCGG_T Cuts at: 6302 6302 Size: 7471 AhdI GACnn_n'nnGTC Cuts at: 6200 6200 Size: 7471 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7471 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7471 AlwNI CAG_nnn'CTG Cuts at: 5214 6786 5214 Size: 1572 5899 ApaLI G'TGCA_C Cuts at: 5528 6684 5528 Size: 1156 6315 ApoI r'AATT_y Cuts at: 1493 2241 2489 2566 2704 2941 3371 3642 4780 Size: 748 248 77 138 237 430 271 1138 Cuts at: 4780 5006 5158 7267 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1138 748 430 271 248 237 226 152 138 77 AseI AT'TA_AT Cuts at: 160 1032 6025 160 Size: 872 4993 1606 Fragments arranged by size: 4993 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 AvaI C'yCGr_G Cuts at: 1870 1943 4796 1870 Size: 73 2853 4545 Fragments arranged by size: 4545 2853 73 BamHI G'GATC_C Cuts at: 2219 4801 5168 2219 Size: 2582 367 4522 Fragments arranged by size: 4522 2582 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4792 6247 618 Size: 325 217 627 3005 1455 1842 Fragments arranged by size: 3005 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3796 4790 1391 Size: 2405 994 4072 Fragments arranged by size: 4072 2405 994 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 2080 2414 3429 4098 4652 4723 Size: 971 142 39 334 1015 669 554 71 Cuts at: 4723 928 Size: 3676 Fragments arranged by size: 3676 1015 971 669 554 334 142 71 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5662 5696 1903 Size: 34 3725 34 3678 Fragments arranged by size: 3725 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3172 5363 6573 3172 Size: 2191 1210 4070 Fragments arranged by size: 4070 2191 1210 BclI T'GATC_A Cuts at: 1548 4221 1548 Size: 2673 4798 BfrBI ATG'CAT Cuts at: 4754 4754 Size: 7471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6082 136 Size: 107 122 71 631 605 4410 1525 Fragments arranged by size: 4410 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7466 7466 Size: 7471 BlpI GC'TnA_GC Cuts at: 3544 4888 3544 Size: 1344 6127 Bme1580I G_kGCm'C Cuts at: 1790 5532 6688 1790 Size: 3742 1156 2573 Fragments arranged by size: 3742 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3711 1318 Size: 2393 5078 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5202 6160 442 Size: 416 53 83 399 24 3785 958 1753 Fragments arranged by size: 3785 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4145 1056 Size: 3089 4382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2748 4111 6131 1239 Size: 1509 1363 2020 2579 Fragments arranged by size: 2579 2020 1509 1363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5596 6461 6759 5596 Size: 865 298 6308 Fragments arranged by size: 6308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2842 6134 882 Size: 617 1343 3292 2219 Fragments arranged by size: 3292 2219 1343 617 BsaAI yAC'GTr Cuts at: 493 1204 5262 493 Size: 711 4058 2702 Fragments arranged by size: 4058 2702 711 BsaBI GATnn'nnATC Cuts at: 7465 7465 Size: 7471 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5278 5660 6295 275 Size: 53 83 186 936 3745 382 635 1451 Fragments arranged by size: 3745 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5904 6302 6576 6723 7134 5904 Size: 398 274 147 411 6241 Fragments arranged by size: 6241 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2837 2867 1178 Size: 30 165 30 693 30 711 30 5782 Fragments arranged by size: 5782 711 693 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 4384 1186 Size: 931 2267 4273 Fragments arranged by size: 4273 2267 931 BseYI C'CCAG_C Cuts at: 6674 7170 6674 Size: 496 6975 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 1163 Size: 573 398 6500 Fragments arranged by size: 6500 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4921 5682 6710 1540 Size: 275 186 2920 761 1028 2301 Fragments arranged by size: 2920 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3170 3545 3796 4252 5532 5617 6688 3170 Size: 375 251 456 1280 85 1071 3953 Fragments arranged by size: 3953 1280 1071 456 375 251 85 BsmI GAATG_Cn' Cuts at: 2897 4982 5075 2897 Size: 2085 93 5293 Fragments arranged by size: 5293 2085 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3170 3545 3796 4252 4790 5532 5617 Size: 399 1380 375 251 456 538 742 85 Cuts at: 5617 6688 1391 Size: 1071 2174 Fragments arranged by size: 2174 1380 1071 742 538 456 399 375 251 85 BspEI T'CCGG_A Cuts at: 7134 7134 Size: 7471 BspHI T'CATG_A Cuts at: 5360 7090 5360 Size: 1730 5741 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4826 844 Size: 3982 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5358 1394 Size: 429 3535 3507 Fragments arranged by size: 3535 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2322 2549 3615 5966 6140 65 Size: 1183 1074 227 1066 2351 174 1396 Fragments arranged by size: 2351 1396 1183 1074 1066 227 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6115 6302 1700 Size: 110 4305 187 2869 Fragments arranged by size: 4305 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2443 3705 96 Size: 2347 1262 3862 Fragments arranged by size: 3862 2347 1262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7471 BssSI C'ACGA_G Cuts at: 5531 6543 5531 Size: 1012 6459 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7471 BstBI TT'CG_AA Cuts at: 6275 6275 Size: 7471 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7471 BstYI r'GATC_y Cuts at: 1972 2219 4801 5168 5553 5570 7022 7108 7120 Size: 247 2582 367 385 17 1452 86 12 Cuts at: 7120 7466 1972 Size: 346 1977 Fragments arranged by size: 2582 1977 1452 385 367 346 247 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6919 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6732 Fragments arranged by size: 6732 683 56 BtsI GCAGTG_nn' Cuts at: 2826 4987 5781 5801 2826 Size: 2161 794 20 4496 Fragments arranged by size: 4496 2161 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7471 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2487 2522 3429 3464 529 Size: 35 1923 35 907 35 4536 Fragments arranged by size: 4536 1923 907 35 35 35 DraI TTT'AAA Cuts at: 3379 3520 3700 3970 4540 4775 3379 Size: 141 180 270 570 235 6075 Fragments arranged by size: 6075 570 270 235 180 141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6478 809 Size: 507 5162 1802 Fragments arranged by size: 5162 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4918 5807 7436 Size: 54 1425 50 275 18 3088 889 1629 Cuts at: 7436 8 Size: 43 Fragments arranged by size: 3088 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4918 1537 Size: 275 3106 4090 Fragments arranged by size: 4090 3106 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3801 5401 1353 Size: 2448 1600 3423 Fragments arranged by size: 3423 2448 1600 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6058 6432 6578 387 Size: 1064 138 4469 374 146 1280 Fragments arranged by size: 4469 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3794 3794 Size: 7471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2408 2748 4111 4452 5534 6131 Size: 393 618 158 340 1363 341 1082 597 Cuts at: 6131 6918 7186 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1363 1082 787 618 597 393 341 340 268 158 EcoRI G'AATT_C Cuts at: 3371 4780 5158 3371 Size: 1409 378 5684 Fragments arranged by size: 5684 1409 378 EcoRV GAT'ATC Cuts at: 4050 4177 4188 4050 Size: 127 11 7333 Fragments arranged by size: 7333 127 11 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2380 3766 4457 2380 Size: 1386 691 5394 Fragments arranged by size: 5394 1386 691 FspI TGC'GCA Cuts at: 5977 5977 Size: 7471 HaeII r_GCGC'y Cuts at: 1678 6618 1678 Size: 4940 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 3290 3322 6112 Size: 32 135 32 1265 32 421 32 2790 Cuts at: 6112 6144 6186 6218 1373 Size: 32 42 32 2626 Fragments arranged by size: 2790 2626 1265 421 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 4815 5061 669 Size: 988 281 945 1932 246 3079 Fragments arranged by size: 3079 1932 988 945 281 246 HindIII A'AGCT_T Cuts at: 748 3513 748 Size: 2765 4706 HpaI GTT'AAC Cuts at: 5061 5061 Size: 7471 KpnI G_GTAC'C Cuts at: 4796 4796 Size: 7471 MfeI C'AATT_G Cuts at: 2873 4212 5070 2873 Size: 1339 858 5274 Fragments arranged by size: 5274 1339 858 MluI A'CGCG_T Cuts at: 7347 7347 Size: 7471 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3170 3873 4214 4610 6585 6769 1305 Size: 1865 703 341 396 1975 184 2007 Fragments arranged by size: 2007 1975 1865 703 396 341 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5649 Fragments arranged by size: 5649 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2793 3046 3218 5429 5788 5947 518 Size: 648 1627 253 172 2211 359 159 2042 Fragments arranged by size: 2211 2042 1627 648 359 253 172 159 MspA1I CmG'CkG Cuts at: 1606 4887 5564 6712 6957 1606 Size: 3281 677 1148 245 2120 Fragments arranged by size: 3281 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6919 NdeI CA'TA_TG Cuts at: 387 2194 387 Size: 1807 5664 NheI G'CTAG_C Cuts at: 1052 4141 1052 Size: 3089 4382 NotI GC'GGCC_GC Cuts at: 4918 4918 Size: 7471 NsiI A_TGCA'T Cuts at: 4756 4756 Size: 7471 NspI r_CATG'y Cuts at: 1132 1229 2131 4450 4498 4829 6374 1132 Size: 97 902 2319 48 331 1545 2229 Fragments arranged by size: 2319 2229 1545 902 331 97 48 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 6748 PciI A'CATG_T Cuts at: 2127 6370 2127 Size: 4243 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2862 1108 Size: 1754 5717 PfoI T'CCnGG_A Cuts at: 2736 2736 Size: 7471 PmeI GTTT'AAAC Cuts at: 4775 4775 Size: 7471 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5540 5572 7053 7085 5540 Size: 32 1481 32 5926 Fragments arranged by size: 5926 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2834 4214 1613 Size: 1221 1380 4870 Fragments arranged by size: 4870 1380 1221 PsiI TTA'TAA Cuts at: 2298 2981 5041 2298 Size: 683 2060 4728 Fragments arranged by size: 4728 2060 683 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3324 3356 3324 Size: 32 7439 PstI C_TGCA'G Cuts at: 830 4823 830 Size: 3993 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7471 SacI G_AGCT'C Cuts at: 3796 3796 Size: 7471 SalI G'TCGA_C Cuts at: 1936 4813 1936 Size: 2877 4594 SapI GCTCTTCn'nnn_ Cuts at: 3801 3801 Size: 7471 SbfI CC_TGCA'GG Cuts at: 4823 4823 Size: 7471 ScaI AGT'ACT Cuts at: 4456 5719 7431 4456 Size: 1263 1712 4496 Fragments arranged by size: 4496 1712 1263 SfcI C'TryA_G Cuts at: 826 1045 2721 2886 3574 4819 5954 6635 6826 Size: 219 1676 165 688 1245 1135 681 191 Cuts at: 6826 826 Size: 1471 Fragments arranged by size: 1676 1471 1245 1135 688 681 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7471 SmaI CCC'GGG Cuts at: 1872 4798 1872 Size: 2926 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5575 6476 6738 820 Size: 197 926 3632 901 262 1553 Fragments arranged by size: 3632 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5262 493 Size: 4769 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7471 SphI G_CATG'C Cuts at: 1132 4450 4498 4829 1132 Size: 3318 48 331 3774 Fragments arranged by size: 3774 3318 331 48 SspI AAT'ATT Cuts at: 5 52 5395 5 Size: 47 5343 2081 Fragments arranged by size: 5343 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7471 StyI C'CwwG_G Cuts at: 513 1065 3590 4910 513 Size: 552 2525 1320 3074 Fragments arranged by size: 3074 2525 1320 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2519 3003 5498 5515 5668 5853 6298 Size: 1255 939 484 2495 17 153 185 445 Cuts at: 6298 325 Size: 1498 Fragments arranged by size: 2495 1498 1255 939 484 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2443 3705 4079 Size: 274 80 33 51 1443 466 1262 374 Cuts at: 4079 4454 5717 7429 96 Size: 375 1263 1712 138 Fragments arranged by size: 1712 1443 1263 1262 466 375 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2096 2128 4228 4260 7431 7463 2096 Size: 32 2100 32 3171 32 2104 Fragments arranged by size: 3171 2104 2100 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7471 XbaI T'CTAG_A Cuts at: 3481 4355 4807 3481 Size: 874 452 6145 Fragments arranged by size: 6145 874 452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7471 XmaI C'CCGG_G Cuts at: 1870 4796 1870 Size: 2926 4545 XmnI GAAnn'nnTTC Cuts at: 5600 5600 Size: 7471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5279 276 Size: 53 83 186 936 3745 2468 Fragments arranged by size: 3745 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI