(Circular) (Six-base) MAPSORT of: pFN21AE0085.seq Check: 6741 from: 1 to: 8956 pFN21AE0085 8956 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5776 5776 Size: 8956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6766 278 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6299 1937 Size: 4362 4594 Acc65I G'GTAC_C Cuts at: 6277 6277 Size: 8956 AclI AA'CG_TT Cuts at: 1563 4716 7083 7456 1563 Size: 3153 2367 373 3063 Fragments arranged by size: 3153 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 3183 4539 4665 4721 5949 7019 8403 Size: 833 718 1356 126 56 1228 1070 1384 Cuts at: 8403 8671 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1356 1228 1070 833 718 268 126 56 AflII C'TTAA_G Cuts at: 820 1017 2431 820 Size: 197 1414 7345 Fragments arranged by size: 7345 1414 197 AflIII A'CryG_T Cuts at: 1203 7855 8832 1203 Size: 6652 977 1327 Fragments arranged by size: 6652 1327 977 AgeI A'CCGG_T Cuts at: 7787 7787 Size: 8956 AhdI GACnn_n'nnGTC Cuts at: 7685 7685 Size: 8956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2754 1184 Size: 1570 7386 AlwNI CAG_nnn'CTG Cuts at: 3827 6699 8271 3827 Size: 2872 1572 4512 Fragments arranged by size: 4512 2872 1572 ApaI G_GGCC'C Cuts at: 2269 2269 Size: 8956 ApaLI G'TGCA_C Cuts at: 7013 8169 7013 Size: 1156 7800 ApoI r'AATT_y Cuts at: 1493 2204 2509 2777 2887 3255 3334 3428 3720 Size: 711 305 268 110 368 79 94 292 Cuts at: 3720 3973 4245 4275 4560 6265 6491 6643 8752 Size: 253 272 30 285 1705 226 152 2109 Cuts at: 8752 1493 Size: 1697 Fragments arranged by size: 2109 1705 1697 711 368 305 292 285 272 268 253 226 152 110 94 79 30 AseI AT'TA_AT Cuts at: 160 1032 4227 7510 160 Size: 872 3195 3283 1606 Fragments arranged by size: 3283 3195 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2080 2268 2301 5778 6281 1870 Size: 73 114 23 188 33 3477 503 4545 Fragments arranged by size: 4545 3477 503 188 114 73 33 23 BamHI G'GATC_C Cuts at: 6286 6653 6286 Size: 367 8589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2130 6277 7732 618 Size: 325 217 627 343 4147 1455 1842 Fragments arranged by size: 4147 1842 1455 627 343 325 217 BanII G_rGCy'C Cuts at: 1391 2068 2081 2269 5379 6275 1391 Size: 677 13 188 3110 896 4072 Fragments arranged by size: 4072 3110 896 677 188 13 BbeI G_GCGC'C Cuts at: 2134 2134 Size: 8956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3645 4685 5367 6093 928 Size: 971 1746 1040 682 726 3791 Fragments arranged by size: 3791 1746 1040 971 726 682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2192 2226 7147 7181 1903 Size: 34 255 34 4921 34 3678 Fragments arranged by size: 4921 3678 255 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6157 6848 8058 6157 Size: 691 1210 7055 Fragments arranged by size: 7055 1210 691 BclI T'GATC_A Cuts at: 1548 1548 Size: 8956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7567 136 Size: 107 122 71 631 605 5895 1525 Fragments arranged by size: 5895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8951 8951 Size: 8956 BlpI GC'TnA_GC Cuts at: 6373 6373 Size: 8956 Bme1580I G_kGCm'C Cuts at: 1790 2269 7017 8173 1790 Size: 479 4748 1156 2573 Fragments arranged by size: 4748 2573 1156 479 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3553 5945 6687 Size: 416 53 83 399 24 2136 2392 742 Cuts at: 6687 7645 442 Size: 958 1753 Fragments arranged by size: 2392 2136 1753 958 742 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4042 5237 5537 5894 7616 1239 Size: 2803 1195 300 357 1722 2579 Fragments arranged by size: 2803 2579 1722 1195 357 300 Bpu10I CC'TnA_GC Cuts at: 5436 6159 5436 Size: 723 8233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2852 2895 4130 5219 7081 7946 8244 2852 Size: 43 1235 1089 1862 865 298 3564 Fragments arranged by size: 3564 1862 1235 1089 865 298 43 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2337 7619 882 Size: 617 838 5282 2219 Fragments arranged by size: 5282 2219 838 617 BsaAI yAC'GTr Cuts at: 493 1204 3944 5954 6747 493 Size: 711 2740 2010 793 2702 Fragments arranged by size: 2740 2702 2010 793 711 BsaBI GATnn'nnATC Cuts at: 3873 8950 3873 Size: 5077 3879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2131 6763 7145 7780 Size: 53 83 186 936 598 4632 382 635 Cuts at: 7780 275 Size: 1451 Fragments arranged by size: 4632 1451 936 635 598 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7389 7787 8061 8208 8619 7389 Size: 398 274 147 411 7726 Fragments arranged by size: 7726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6237 6267 1178 Size: 30 165 30 4834 30 3867 Fragments arranged by size: 4834 3867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4046 4901 5490 1186 Size: 2860 855 589 4652 Fragments arranged by size: 4652 2860 855 589 BseYI C'CCAG_C Cuts at: 2339 8159 8655 2339 Size: 5820 496 2640 Fragments arranged by size: 5820 2640 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4890 5810 1163 Size: 573 3154 920 4309 Fragments arranged by size: 4309 3154 920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6406 7167 8195 1540 Size: 275 186 4405 761 1028 2301 Fragments arranged by size: 4405 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5379 5472 7017 7102 8173 5379 Size: 93 1545 85 1071 6162 Fragments arranged by size: 6162 1545 1071 93 85 BsmI GAATG_Cn' Cuts at: 2743 5348 5982 6467 6560 2743 Size: 2605 634 485 93 5139 Fragments arranged by size: 5139 2605 634 485 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2081 2269 5379 5472 6275 7017 Size: 399 278 13 188 3110 93 803 742 Cuts at: 7017 7102 8173 1391 Size: 85 1071 2174 Fragments arranged by size: 3110 2174 1071 803 742 399 278 188 93 85 13 BspEI T'CCGG_A Cuts at: 8619 8619 Size: 8956 BspHI T'CATG_A Cuts at: 6845 8575 6845 Size: 1730 7226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5776 6311 844 Size: 4932 535 3489 Fragments arranged by size: 4932 3489 535 BsrBI CCG'CTC Cuts at: 1394 1823 6843 1394 Size: 429 5020 3507 Fragments arranged by size: 5020 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5061 5724 7451 7625 65 Size: 1183 3813 663 1727 174 1396 Fragments arranged by size: 3813 1727 1396 1183 663 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7600 7787 1700 Size: 110 5790 187 2869 Fragments arranged by size: 5790 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8956 BssHII G'CGCG_C Cuts at: 1925 2085 2313 1925 Size: 160 228 8568 Fragments arranged by size: 8568 228 160 BssSI C'ACGA_G Cuts at: 7016 8028 7016 Size: 1012 7944 BstAPI GCAn_nnn'nTGC Cuts at: 794 2423 3654 4163 794 Size: 1629 1231 509 5587 Fragments arranged by size: 5587 1629 1231 509 BstBI TT'CG_AA Cuts at: 2202 7760 2202 Size: 5558 3398 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8956 BstXI CCAn_nnnn'nTGG Cuts at: 4066 5755 4066 Size: 1689 7267 BstYI r'GATC_y Cuts at: 1972 5320 6286 6653 7038 7055 8507 8593 8605 Size: 3348 966 367 385 17 1452 86 12 Cuts at: 8605 8951 1972 Size: 346 1977 Fragments arranged by size: 3348 1977 1452 966 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2192 513 Size: 552 939 188 7277 Fragments arranged by size: 7277 939 552 188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8217 Fragments arranged by size: 8217 683 56 BtsI GCAGTG_nn' Cuts at: 2167 3333 4953 6472 7266 7286 2167 Size: 1166 1620 1519 794 20 3837 Fragments arranged by size: 3837 1620 1519 1166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8921 DraI TTT'AAA Cuts at: 3592 4357 4494 6260 3592 Size: 765 137 1766 6288 Fragments arranged by size: 6288 1766 765 137 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7963 809 Size: 507 6647 1802 Fragments arranged by size: 6647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6403 7292 8921 Size: 54 1425 50 275 18 4573 889 1629 Cuts at: 8921 8 Size: 43 Fragments arranged by size: 4573 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6403 1537 Size: 275 4591 4090 Fragments arranged by size: 4591 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2475 3090 4641 4740 4989 6886 1353 Size: 1041 81 615 1551 99 249 1897 3423 Fragments arranged by size: 3423 1897 1551 1041 615 249 99 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7543 7917 8063 387 Size: 1064 138 5954 374 146 1280 Fragments arranged by size: 5954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5377 5377 Size: 8956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 3183 4042 4539 4665 4721 5237 Size: 393 833 718 859 497 126 56 516 Cuts at: 5237 5537 5894 5949 7019 7616 8403 8671 1239 Size: 300 357 55 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 859 833 787 718 597 516 497 393 357 300 268 126 56 55 EcoO109I rG'GnC_Cy Cuts at: 2265 2265 Size: 8956 EcoRI G'AATT_C Cuts at: 2204 6265 6643 2204 Size: 4061 378 4517 Fragments arranged by size: 4517 4061 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3043 5615 6251 3043 Size: 2572 636 5748 Fragments arranged by size: 5748 2572 636 FspI TGC'GCA Cuts at: 7462 7462 Size: 8956 HaeII r_GCGC'y Cuts at: 1678 2134 8103 1678 Size: 456 5969 2531 Fragments arranged by size: 5969 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3736 3768 4195 4227 4669 Size: 32 135 32 2164 32 427 32 442 Cuts at: 4669 4701 5071 5103 5548 5580 6037 6069 7597 Size: 32 370 32 445 32 457 32 1528 Cuts at: 7597 7629 7671 7703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2164 1528 457 445 442 427 370 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3503 3991 5648 6300 6546 669 Size: 988 281 1565 488 1657 652 246 3079 Fragments arranged by size: 3079 1657 1565 988 652 488 281 246 HindIII A'AGCT_T Cuts at: 748 3108 3698 3728 4047 4488 6027 748 Size: 2360 590 30 319 441 1539 3677 Fragments arranged by size: 3677 2360 1539 590 441 319 30 HpaI GTT'AAC Cuts at: 3991 6546 3991 Size: 2555 6401 KasI G'GCGC_C Cuts at: 2130 2130 Size: 8956 KpnI G_GTAC'C Cuts at: 6281 6281 Size: 8956 MfeI C'AATT_G Cuts at: 4595 6555 4595 Size: 1960 6996 MluI A'CGCG_T Cuts at: 8832 8832 Size: 8956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3139 8070 8254 1305 Size: 1834 4931 184 2007 Fragments arranged by size: 4931 2007 1834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7134 Fragments arranged by size: 7134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3326 6914 7273 7432 518 Size: 648 2160 3588 359 159 2042 Fragments arranged by size: 3588 2160 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 4021 4120 4865 4904 5330 6372 7049 Size: 588 1827 99 745 39 426 1042 677 Cuts at: 7049 8197 8442 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1827 1148 1042 745 677 588 426 245 99 39 NarI GG'CG_CC Cuts at: 2131 2131 Size: 8956 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7465 Fragments arranged by size: 7465 939 552 NdeI CA'TA_TG Cuts at: 387 6036 387 Size: 5649 3307 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8956 NotI GC'GGCC_GC Cuts at: 6403 6403 Size: 8956 NspI r_CATG'y Cuts at: 1132 1229 6314 7859 1132 Size: 97 5085 1545 2229 Fragments arranged by size: 5085 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8956 PciI A'CATG_T Cuts at: 7855 7855 Size: 8956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2962 1108 Size: 1854 7102 PfoI T'CCnGG_A Cuts at: 5253 5253 Size: 8956 PmeI GTTT'AAAC Cuts at: 6260 6260 Size: 8956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7025 7057 8538 8570 7025 Size: 32 1481 32 7411 Fragments arranged by size: 7411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8956 PsiI TTA'TAA Cuts at: 2885 5618 6526 2885 Size: 2733 908 5315 Fragments arranged by size: 5315 2733 908 PspOMI G'GGCC_C Cuts at: 2265 2265 Size: 8956 PspXI vC'TCGA_Gb Cuts at: 2301 2301 Size: 8956 PstI C_TGCA'G Cuts at: 830 6308 830 Size: 5478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8956 PvuII CAG'CTG Cuts at: 4021 4120 4865 4904 5330 4021 Size: 99 745 39 426 7647 Fragments arranged by size: 7647 745 426 99 39 SacI G_AGCT'C Cuts at: 5379 5379 Size: 8956 SacII CC_GC'GG Cuts at: 2195 2195 Size: 8956 SalI G'TCGA_C Cuts at: 1936 6298 1936 Size: 4362 4594 SapI GCTCTTCn'nnn_ Cuts at: 3090 4989 3090 Size: 1899 7057 SbfI CC_TGCA'GG Cuts at: 6308 6308 Size: 8956 ScaI AGT'ACT Cuts at: 5155 5196 5239 7204 8916 5155 Size: 41 43 1965 1712 5195 Fragments arranged by size: 5195 1965 1712 43 41 SfcI C'TryA_G Cuts at: 826 1045 4724 5273 6304 7439 8120 8311 826 Size: 219 3679 549 1031 1135 681 191 1471 Fragments arranged by size: 3679 1471 1135 1031 681 549 219 191 SfoI GGC'GCC Cuts at: 2132 2132 Size: 8956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8956 SmaI CCC'GGG Cuts at: 1872 2059 2270 5780 6283 1872 Size: 187 211 3510 503 4545 Fragments arranged by size: 4545 3510 503 211 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 2301 2431 2831 2910 4109 5198 Size: 197 926 358 130 400 79 1199 1089 Cuts at: 5198 7060 7961 8223 820 Size: 1862 901 262 1553 Fragments arranged by size: 1862 1553 1199 1089 926 901 400 358 262 197 130 79 SnaBI TAC'GTA Cuts at: 493 3944 6747 493 Size: 3451 2803 2702 Fragments arranged by size: 3451 2803 2702 SpeI A'CTAG_T Cuts at: 152 4007 152 Size: 3855 5101 SphI G_CATG'C Cuts at: 1132 6314 1132 Size: 5182 3774 SspI AAT'ATT Cuts at: 5 52 3174 4225 6880 5 Size: 47 3122 1051 2655 2081 Fragments arranged by size: 3122 2655 2081 1051 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8956 StyI C'CwwG_G Cuts at: 513 1065 2004 6395 513 Size: 552 939 4391 3074 Fragments arranged by size: 4391 3074 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 6983 7000 7153 7338 7783 325 Size: 1255 762 4641 17 153 185 445 1498 Fragments arranged by size: 4641 1498 1255 762 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4986 5153 5194 Size: 274 80 33 51 1443 3009 167 41 Cuts at: 5194 5237 5269 5721 5736 7202 8914 96 Size: 43 32 452 15 1466 1712 138 Fragments arranged by size: 3009 1712 1466 1443 452 274 167 138 80 51 43 41 33 32 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 8916 8948 6237 Size: 32 2647 32 6245 Fragments arranged by size: 6245 2647 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4375 1316 Size: 3059 5897 XbaI T'CTAG_A Cuts at: 3365 4213 6292 3365 Size: 848 2079 6029 Fragments arranged by size: 6029 2079 848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2347 Size: 8956 XhoI C'TCGA_G Cuts at: 1943 2301 1943 Size: 358 8598 XmaI C'CCGG_G Cuts at: 1870 2057 2268 5778 6281 1870 Size: 187 211 3510 503 4545 Fragments arranged by size: 4545 3510 503 211 187 XmnI GAAnn'nnTTC Cuts at: 2787 3153 5598 7085 2787 Size: 366 2445 1487 4658 Fragments arranged by size: 4658 2445 1487 366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6764 276 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SanDI SexAI SfiI SrfI SwaI