Restriction map for
pFN21AE0084
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspEI AvrII BamHI
BbrPI BciVI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstXI
BstZ17I Bsu36I ClaI DraI DrdI
Eam1105I Eco47III Eco81I EcoRV EcoT22I
FseI HpaI MaeI MamI MfeI
MroI MunI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 853 GACGTC GACGT^C
Acc65I 2492 GGTACC G^GTACC
AccI 835 GTCTAC GT^[AC][GT]AC
AflIII 377 ACGCGT A^C[GA][CT]GT
ApaI 2301 GGGCCC GGGCC^C
Asp700I 1509 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2492 GGTACC G^GTACC
AspHI 1129 GAGCTC G[AT]GC[AT]^C
AspI 2113 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 704 TGCGCA TGC^GCA
BbeI 135 GGCGCC GGCGC^C
BbvCI 2207 CCTCAGC CCTCAGC(-5/-2)
BcgI 2269 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 932 TGATCA T^GATCA
BsaWI 722 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1129 GAGCTC G[AT]GC[AT]^C
BsmI 1768 GCATTC GAATGC(1/-1)
BsrBI 118 GAGCGG CCGCTC(-3/-3)
BsrDI 656 GCAATG GCAATG(2/0)
BssHII 13 GCGCGC G^CGCGC
BstAPI 696 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 2312 CGGACCG CG^G[AT]CCG
DraIII 2098 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2255 CTCTTC CTCTTC(1/4)
EcoNI 2496 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1568 GAATTC G^AATTC
FbaI 932 TGATCA T^GATCA
FspI 704 TGCGCA TGC^GCA
HincII 2383 GTCAAC GT[CT]^[GA]AC
HindII 2383 GTCAAC GT[CT]^[GA]AC
HindIII 742 AAGCTT A^AGCTT
KasI 135 GGCGCC G^GCGCC
KpnI 2492 GGTACC GGTAC^C
Ksp632I 2255 CTCTTC CTCTTC(1/4)
KspI 352 CCGCGG CCGC^GG
MluI 377 ACGCGT A^CGCGT
MslI 606 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 106 GCCGGC GCC^GGC
NarI 135 GGCGCC GG^CGCC
NcoI 1484 CCATGG C^CATGG
NdeI 1808 CATATG CA^TATG
NgoMIV 106 GCCGGC G^CCGGC
NotI 215 GCGGCCGC GC^GGCCGC
PflFI 2113 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 2312 CGGACCG CG^G[AT]CCG
SacI 1129 GAGCTC GAGCT^C
SacII 352 CCGCGG CCGC^GG
SbfI 2295 CCTGCAGG CCTGCA^GG
SfoI 135 GGCGCC GGC^GCC
SphI 707 GCATGC GCATG^C
Sse8387I 2295 CCTGCAGG CCTGCA^GG
StuI 1615 AGGCCT AGG^CCT
Tth111I 2113 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1259 CCAGCCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1509 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1568 GAATTC [GA]^AATT[CT]
1597 AAATTC
AlwNI 697 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1121 CAGCTGCTG
ApoI 1568 GAATTC [GA]^AATT[CT]
1597 AAATTC
BsgI 1075 GTGCAG GTGCAG(16/14)
1954 GTGCAG
BsiEI 104 CGGCCG CG[GA][CT]^CG
216 CGGCCG
BsrFI 106 GCCGGC [GA]^CCGG[CT]
311 ACCGGC
BstEII 1477 GGTGACC G^GT[ACGT]ACC
2103 GGTGACC
BstPI 1477 GGTGACC G^GT[ACGT]ACC
2103 GGTGACC
BstYI 521 GGATCT [GA]^GATC[CT]
698 AGATCC
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
311 ACCGGC
EagI 104 CGGCCG C^GGCCG
216 CGGCCG
EclXI 104 CGGCCG C^GGCCG
216 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
216 CGGCCG
EcoO65I 1477 GGTGACC G^GT[ACGT]ACC
2103 GGTGACC
MflI 521 GGATCT [GA]^GATC[CT]
698 AGATCC
MseI 1012 TTAA T^TAA
1396 TTAA
PleI 2253 GACTC GAGTC(4/5)
2263 GACTC
PstI 694 CTGCAG CTGCA^G
2296 CTGCAG
SmlI 7 CTCAAG C^T[CT][GA]AG
1069 CTCAAG
Tru9I 1012 TTAA T^TAA
1396 TTAA
XhoII 521 GGATCT [GA]^GATC[CT]
698 AGATCC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I