(Circular) (Six-base) MAPSORT of: pFN21AE0084.seq Check: 9461 from: 1 to: 7321 pFN21AE0084 7321 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3946 3946 Size: 7321 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2862 5131 278 Size: 53 83 186 936 1326 2269 2468 Fragments arranged by size: 2468 2269 1326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2841 4664 1937 Size: 904 1823 4594 Fragments arranged by size: 4594 1823 904 Acc65I G'GTAC_C Cuts at: 4497 4642 4497 Size: 145 7176 AclI AA'CG_TT Cuts at: 1563 5448 5821 1563 Size: 3885 373 3063 Fragments arranged by size: 3885 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 3220 3486 3569 3680 4026 4408 5384 Size: 965 623 266 83 111 346 382 976 Cuts at: 5384 6768 7036 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 976 965 623 382 346 268 266 111 83 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7124 AflIII A'CryG_T Cuts at: 1203 2382 6220 7197 1203 Size: 1179 3838 977 1327 Fragments arranged by size: 3838 1327 1179 977 AgeI A'CCGG_T Cuts at: 6152 6152 Size: 7321 AhdI GACnn_n'nnGTC Cuts at: 6050 6050 Size: 7321 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3672 1184 Size: 2488 4833 AlwNI CAG_nnn'CTG Cuts at: 2707 3131 5064 6636 2707 Size: 424 1933 1572 3392 Fragments arranged by size: 3392 1933 1572 424 ApaI G_GGCC'C Cuts at: 4310 4310 Size: 7321 ApaLI G'TGCA_C Cuts at: 5378 6534 5378 Size: 1156 6165 ApoI r'AATT_y Cuts at: 1493 3573 3602 4630 4856 5008 7117 1493 Size: 2080 29 1028 226 152 2109 1697 Fragments arranged by size: 2109 2080 1697 1028 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 5875 160 Size: 872 4843 1606 Fragments arranged by size: 4843 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 AvaI C'yCGr_G Cuts at: 1870 1943 2174 2458 4167 4272 4646 1870 Size: 73 231 284 1709 105 374 4545 Fragments arranged by size: 4545 1709 374 284 231 105 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2791 2824 2791 Size: 33 7288 BamHI G'GATC_C Cuts at: 4651 5018 4651 Size: 367 6954 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 2738 3989 4497 4642 Size: 325 217 627 353 598 1251 508 145 Cuts at: 4642 6097 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1251 627 598 508 353 325 217 145 BanII G_rGCy'C Cuts at: 1391 2013 2265 2759 3138 4310 4640 1391 Size: 622 252 494 379 1172 330 4072 Fragments arranged by size: 4072 1172 622 494 379 330 252 BbeI G_GCGC'C Cuts at: 2144 2144 Size: 7321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3504 3537 3852 928 Size: 971 1605 33 315 4397 Fragments arranged by size: 4397 1605 971 315 33 BbvCI CC'TCA_GC Cuts at: 4213 4213 Size: 7321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4263 4297 5512 5546 1903 Size: 34 2326 34 1215 34 3678 Fragments arranged by size: 3678 2326 1215 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5213 6423 5213 Size: 1210 6111 BclI T'GATC_A Cuts at: 1548 2937 1548 Size: 1389 5932 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5932 136 Size: 107 122 71 631 605 4260 1525 Fragments arranged by size: 4260 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7316 7316 Size: 7321 BlpI GC'TnA_GC Cuts at: 2282 2758 3132 4738 2282 Size: 476 374 1606 4865 Fragments arranged by size: 4865 1606 476 374 Bme1580I G_kGCm'C Cuts at: 1790 2773 3992 4310 5382 6538 1790 Size: 983 1219 318 1072 1156 2573 Fragments arranged by size: 2573 1219 1156 1072 983 318 BmgBI CAC'GTC Cuts at: 1318 2152 1318 Size: 834 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2285 4173 5052 Size: 416 53 83 399 24 868 1888 879 Cuts at: 5052 6010 442 Size: 958 1753 Fragments arranged by size: 1888 1753 958 879 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7321 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2473 2473 Size: 7321 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2369 2501 3172 3272 4424 5981 1239 Size: 992 138 132 671 100 1152 1557 2579 Fragments arranged by size: 2579 1557 1152 992 671 138 132 100 Bpu10I CC'TnA_GC Cuts at: 4213 4213 Size: 7321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1997 3059 5446 6311 6609 1997 Size: 1062 2387 865 298 2709 Fragments arranged by size: 2709 2387 1062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 2900 3284 4416 5984 882 Size: 617 848 553 384 1132 1568 2219 Fragments arranged by size: 2219 1568 1132 848 617 553 384 BsaAI yAC'GTr Cuts at: 493 1204 5112 493 Size: 711 3908 2702 Fragments arranged by size: 3908 2702 711 BsaBI GATnn'nnATC Cuts at: 7315 7315 Size: 7321 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 2343 2859 3024 Size: 53 83 186 936 608 202 516 165 Cuts at: 3024 5128 5510 6145 275 Size: 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 608 516 382 202 186 165 83 53 BsaWI w'CCGG_w Cuts at: 2727 5754 6152 6426 6573 6984 2727 Size: 3027 398 274 147 411 3064 Fragments arranged by size: 3064 3027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4310 4340 4481 4511 1178 Size: 30 165 30 2907 30 141 30 3988 Fragments arranged by size: 3988 2907 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2900 2912 3985 4194 4412 1186 Size: 949 765 12 1073 209 218 4095 Fragments arranged by size: 4095 1073 949 765 218 209 12 BseYI C'CCAG_C Cuts at: 2086 2364 3274 3789 4544 6524 7020 2086 Size: 278 910 515 755 1980 496 2387 Fragments arranged by size: 2387 1980 910 755 515 496 278 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3101 3980 1163 Size: 573 1365 879 4504 Fragments arranged by size: 4504 1365 879 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2224 4771 5532 6560 1540 Size: 275 186 111 112 2547 761 1028 2301 Fragments arranged by size: 2547 2301 1028 761 275 186 112 111 BsiHKAI G_wGCw'C Cuts at: 3138 5382 5467 6538 3138 Size: 2244 85 1071 3921 Fragments arranged by size: 3921 2244 1071 85 BsmI GAATG_Cn' Cuts at: 3773 4832 4925 3773 Size: 1059 93 6169 Fragments arranged by size: 6169 1059 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3031 3226 3593 4333 4508 3031 Size: 195 367 740 175 5844 Fragments arranged by size: 5844 740 367 195 175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2265 2759 2773 3138 3992 4310 Size: 399 223 252 494 14 365 854 318 Cuts at: 4310 4640 5382 5467 6538 1391 Size: 330 742 85 1071 2174 Fragments arranged by size: 2174 1071 854 742 494 399 365 330 318 252 223 85 14 BspEI T'CCGG_A Cuts at: 6984 6984 Size: 7321 BspHI T'CATG_A Cuts at: 5210 6940 5210 Size: 1730 5591 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3946 3952 4170 4308 4676 844 Size: 3102 6 218 138 368 3489 Fragments arranged by size: 3489 3102 368 218 138 6 BsrBI CCG'CTC Cuts at: 1394 1823 2125 5208 1394 Size: 429 302 3083 3507 Fragments arranged by size: 3507 3083 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 2668 5816 5990 65 Size: 1183 1420 3148 174 1396 Fragments arranged by size: 3148 1420 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2316 5965 6152 1700 Size: 110 301 205 3649 187 2869 Fragments arranged by size: 3649 2869 301 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7321 BssHII G'CGCG_C Cuts at: 1925 2018 1925 Size: 93 7228 BssSI C'ACGA_G Cuts at: 3309 3920 4394 5381 6393 3309 Size: 611 474 987 1012 4237 Fragments arranged by size: 4237 1012 987 611 474 BstAPI GCAn_nnn'nTGC Cuts at: 794 2707 794 Size: 1913 5408 BstBI TT'CG_AA Cuts at: 6125 6125 Size: 7321 BstEII G'GTnAC_C Cuts at: 1184 3482 4108 1184 Size: 2298 626 4397 Fragments arranged by size: 4397 2298 626 BstYI r'GATC_y Cuts at: 1972 2526 2703 4651 5018 5403 5420 6872 6958 Size: 554 177 1948 367 385 17 1452 86 Cuts at: 6958 6970 7316 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1948 1452 554 385 367 346 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2357 3489 4239 513 Size: 552 939 353 1132 750 3595 Fragments arranged by size: 3595 1132 939 750 552 353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6582 Fragments arranged by size: 6582 683 56 BtsI GCAGTG_nn' Cuts at: 4837 5631 5651 4837 Size: 794 20 6507 Fragments arranged by size: 6507 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7321 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4209 4244 529 Size: 35 3645 35 3606 Fragments arranged by size: 3645 3606 35 35 DraI TTT'AAA Cuts at: 4625 4625 Size: 7321 DraIII CAC_nnn'GTG Cuts at: 4108 4108 Size: 7321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6328 809 Size: 507 5012 1802 Fragments arranged by size: 5012 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2221 2268 Size: 54 1425 50 275 18 279 112 47 Cuts at: 2268 2670 2946 4768 5657 7286 8 Size: 402 276 1822 889 1629 43 Fragments arranged by size: 1822 1629 1425 889 402 279 276 275 112 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 2221 4768 1537 Size: 275 297 112 2547 4090 Fragments arranged by size: 4090 2547 297 275 112 EarI CTCTTCn'nnn_ Cuts at: 1353 4266 5251 1353 Size: 2913 985 3423 Fragments arranged by size: 3423 2913 985 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 3509 5908 6282 6428 387 Size: 1064 138 490 1430 2399 374 146 1280 Fragments arranged by size: 2399 1430 1280 1064 490 374 146 138 EcoICRI GAG'CTC Cuts at: 3136 3136 Size: 7321 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2369 2501 2597 3172 3220 3272 Size: 393 599 138 132 96 575 48 52 Cuts at: 3272 3486 3569 3680 4026 4408 4424 5384 5981 Size: 214 83 111 346 382 16 960 597 Cuts at: 5981 6768 7036 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 787 599 597 575 393 382 346 268 214 138 132 111 96 83 52 48 16 EcoNI CCTnn'n_nnAGG Cuts at: 4505 4505 Size: 7321 EcoO109I rG'GnC_Cy Cuts at: 2199 2244 2932 4076 4175 4306 4307 2199 Size: 45 688 1144 99 131 1 5213 Fragments arranged by size: 5213 1144 688 131 99 45 1 EcoRI G'AATT_C Cuts at: 3573 4630 5008 3573 Size: 1057 378 5886 Fragments arranged by size: 5886 1057 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3931 3931 Size: 7321 FspI TGC'GCA Cuts at: 2711 5827 2711 Size: 3116 4205 HaeII r_GCGC'y Cuts at: 1678 2144 2174 2372 6468 1678 Size: 466 30 198 4096 2531 Fragments arranged by size: 4096 2531 466 198 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3245 3277 4310 4332 4342 Size: 32 135 32 1673 32 1033 22 10 Cuts at: 4342 4364 5962 5994 6036 6068 1373 Size: 22 1598 32 42 32 2626 Fragments arranged by size: 2626 1673 1598 1033 135 42 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 4390 4665 4911 669 Size: 988 281 2452 275 246 3079 Fragments arranged by size: 3079 2452 988 281 275 246 HindIII A'AGCT_T Cuts at: 748 2747 748 Size: 1999 5322 HpaI GTT'AAC Cuts at: 4911 4911 Size: 7321 KasI G'GCGC_C Cuts at: 2140 2140 Size: 7321 KpnI G_GTAC'C Cuts at: 4501 4646 4501 Size: 145 7176 MfeI C'AATT_G Cuts at: 4920 4920 Size: 7321 MluI A'CGCG_T Cuts at: 2382 7197 2382 Size: 4815 2506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 6435 6619 1305 Size: 1281 3849 184 2007 Fragments arranged by size: 3849 2007 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 2672 2948 10 Size: 54 1425 343 438 402 276 4383 Fragments arranged by size: 4383 1425 438 402 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 5279 5638 5797 518 Size: 648 1449 2664 359 159 2042 Fragments arranged by size: 2664 2042 1449 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2239 2281 2359 3047 3128 3250 4004 4548 Size: 633 42 78 688 81 122 754 544 Cuts at: 4548 4737 5414 6562 6807 1606 Size: 189 677 1148 245 2120 Fragments arranged by size: 2120 1148 754 688 677 633 544 245 189 122 81 78 42 NaeI GCC'GGC Cuts at: 2113 2113 Size: 7321 NarI GG'CG_CC Cuts at: 2141 2141 Size: 7321 NcoI C'CATG_G Cuts at: 513 1065 2004 3489 513 Size: 552 939 1485 4345 Fragments arranged by size: 4345 1485 939 552 NdeI CA'TA_TG Cuts at: 387 3814 387 Size: 3427 3894 NgoMIV G'CCGG_C Cuts at: 2111 2111 Size: 7321 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7321 NotI GC'GGCC_GC Cuts at: 2221 4768 2221 Size: 2547 4774 NspI r_CATG'y Cuts at: 1132 1229 2605 2716 3676 3937 4679 6224 1132 Size: 97 1376 111 960 261 742 1545 2229 Fragments arranged by size: 2229 1545 1376 960 742 261 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7321 PciI A'CATG_T Cuts at: 6220 6220 Size: 7321 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 2982 3274 1108 Size: 1278 596 292 5155 Fragments arranged by size: 5155 1278 596 292 PfoI T'CCnGG_A Cuts at: 3680 3680 Size: 7321 PmeI GTTT'AAAC Cuts at: 4625 4625 Size: 7321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5390 5422 6903 6935 5390 Size: 32 1481 32 5776 Fragments arranged by size: 5776 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2199 2244 4175 2199 Size: 45 1931 5345 Fragments arranged by size: 5345 1931 45 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7321 PsiI TTA'TAA Cuts at: 4891 4891 Size: 7321 PspOMI G'GGCC_C Cuts at: 4306 4306 Size: 7321 PstI C_TGCA'G Cuts at: 830 2703 4305 4673 830 Size: 1873 1602 368 3478 Fragments arranged by size: 3478 1873 1602 368 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7321 PvuII CAG'CTG Cuts at: 3128 3250 4004 3128 Size: 122 754 6445 Fragments arranged by size: 6445 754 122 RsrII CG'GwC_CG Cuts at: 4318 4318 Size: 7321 SacI G_AGCT'C Cuts at: 3138 3138 Size: 7321 SacII CC_GC'GG Cuts at: 2360 2360 Size: 7321 SalI G'TCGA_C Cuts at: 1936 4663 1936 Size: 2727 4594 SanDI GG'GwC_CC Cuts at: 2199 4175 2199 Size: 1976 5345 SbfI CC_TGCA'GG Cuts at: 4305 4673 4305 Size: 368 6953 ScaI AGT'ACT Cuts at: 5569 7281 5569 Size: 1712 5609 SexAI A'CCwGG_T Cuts at: 2844 3186 4104 2844 Size: 342 918 6061 Fragments arranged by size: 6061 918 342 SfcI C'TryA_G Cuts at: 826 1045 2699 4093 4301 4669 5804 6485 6676 Size: 219 1654 1394 208 368 1135 681 191 Cuts at: 6676 826 Size: 1471 Fragments arranged by size: 1654 1471 1394 1135 681 368 219 208 191 SfoI GGC'GCC Cuts at: 2142 2142 Size: 7321 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7321 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7321 SmaI CCC'GGG Cuts at: 1872 4648 1872 Size: 2776 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 3074 5425 6326 6588 820 Size: 197 926 69 1062 2351 901 262 1553 Fragments arranged by size: 2351 1553 1062 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 5112 493 Size: 4619 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7321 SphI G_CATG'C Cuts at: 1132 2716 4679 1132 Size: 1584 1963 3774 Fragments arranged by size: 3774 1963 1584 SspI AAT'ATT Cuts at: 5 52 5245 5 Size: 47 5193 2081 Fragments arranged by size: 5193 2081 47 StuI AGG'CCT Cuts at: 1517 3622 1517 Size: 2105 5216 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 2656 3489 3984 4760 513 Size: 552 939 162 490 833 495 776 3074 Fragments arranged by size: 3074 939 833 776 552 495 490 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2989 4335 4375 5348 5365 5518 5703 Size: 1255 1409 1346 40 973 17 153 185 Cuts at: 5703 6148 325 Size: 445 1498 Fragments arranged by size: 1498 1409 1346 1255 973 445 185 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5567 7279 96 Size: 274 80 33 51 1443 3590 1712 138 Fragments arranged by size: 3590 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7281 7313 7281 Size: 32 7289 Tth111I GACn'n_nGTC Cuts at: 1316 4121 1316 Size: 2805 4516 XbaI T'CTAG_A Cuts at: 4657 4657 Size: 7321 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3271 3271 Size: 7321 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7321 XmaI C'CCGG_G Cuts at: 1870 4646 1870 Size: 2776 4545 XmnI GAAnn'nnTTC Cuts at: 3518 5450 3518 Size: 1932 5389 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2860 5129 276 Size: 53 83 186 936 1326 2269 2468 Fragments arranged by size: 2468 2269 1326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SapI SfiI SrfI SwaI