Restriction map for
pFN21AE0078
(Circular) (Six-base) MAPSORT of: pFN21AE0078.seq Check: 7443 from: 1 to: 7261
pFN21AE0078 7261 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2800 4351 4443 2800
Size: 1551 92 5618
Fragments arranged by size:
5618 1551 92
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2781 5071 278
Size: 53 83 186 936 1245 2290 2468
Fragments arranged by size:
2468 2290 1245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3183 4604 1937
Size: 1246 1421 4594
Fragments arranged by size:
4594 1421 1246
Acc65I G'GTAC_C
Cuts at: 4098 4582 4098
Size: 484 6777
AclI AA'CG_TT
Cuts at: 1563 5388 5761 1563
Size: 3825 373 3063
Fragments arranged by size:
3825 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2458 3212 3392 3473 5324 6708 6976 1632
Size: 826 754 180 81 1851 1384 268 1917
Fragments arranged by size:
1917 1851 1384 826 754 268 180 81
AfeI AGC'GCT
Cuts at: 3110 3110
Size: 7261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7064
AflIII A'CryG_T
Cuts at: 1203 3291 3468 6160 7137 1203
Size: 2088 177 2692 977 1327
Fragments arranged by size:
2692 2088 1327 977 177
AgeI A'CCGG_T
Cuts at: 6092 6092
Size: 7261
AhdI GACnn_n'nnGTC
Cuts at: 5990 5990
Size: 7261
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2537 3152 1184
Size: 1353 615 5293
Fragments arranged by size:
5293 1353 615
AlwNI CAG_nnn'CTG
Cuts at: 2037 2671 3085 3214 3283 5004 6576 2037
Size: 634 414 129 69 1721 1572 2722
Fragments arranged by size:
2722 1721 1572 634 414 129 69
ApaI G_GGCC'C
Cuts at: 2015 2068 2436 3819 4041 4505 2015
Size: 53 368 1383 222 464 4771
Fragments arranged by size:
4771 1383 464 368 222 53
ApaLI G'TGCA_C
Cuts at: 2188 2834 4090 4163 5318 6474 2188
Size: 646 1256 73 1155 1156 2975
Fragments arranged by size:
2975 1256 1156 1155 646 73
ApoI r'AATT_y
Cuts at: 1493 4570 4796 4948 7057 1493
Size: 3077 226 152 2109 1697
Fragments arranged by size:
3077 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5815 160
Size: 872 4783 1606
Fragments arranged by size:
4783 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2532 3808 4481 4586 1870
Size: 73 333 256 1276 673 105 4545
Fragments arranged by size:
4545 1276 673 333 256 105 73
BamHI G'GATC_C
Cuts at: 4591 4958 4591
Size: 367 6894
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 2987 4098 4391 4582
Size: 325 217 627 944 256 1111 293 191
Cuts at: 4582 6037 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1111 944 627 325 293 256
217 191
BanII G_rGCy'C
Cuts at: 1391 2015 2044 2068 2436 2451 3395 3819 4041
Size: 624 29 24 368 15 944 424 222
Cuts at: 4041 4473 4505 4580 1391
Size: 432 32 75 4072
Fragments arranged by size:
4072 944 624 432 424 368 222 75
32 29 24 15
BbeI G_GCGC'C
Cuts at: 4395 4395
Size: 7261
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3026 928
Size: 971 1127 5163
Fragments arranged by size:
5163 1127 971
BbvCI CC'TCA_GC
Cuts at: 2030 3860 2030
Size: 1830 5431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2695 2729 5452 5486 1903
Size: 34 50 34 674 34 2723 34 3678
Fragments arranged by size:
3678 2723 674 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5153 6363 5153
Size: 1210 6051
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7261
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2147 2667 2906
Size: 107 122 71 631 605 475 520 239
Cuts at: 2906 3519 4223 4269 4310 5872 136
Size: 613 704 46 41 1562 1525
Fragments arranged by size:
1562 1525 704 631 613 605 520 475
239 122 107 71 46 41
BglII A'GATC_T
Cuts at: 3975 7256 3975
Size: 3281 3980
BlpI GC'TnA_GC
Cuts at: 2525 3731 4678 2525
Size: 1206 947 5108
Fragments arranged by size:
5108 1206 947
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2068 2192 2224 2436 2568 2838 2963
Size: 225 53 124 32 212 132 270 125
Cuts at: 2963 2992 3402 3819 4041 4094 4167 4247 4288
Size: 29 410 417 222 53 73 80 41
Cuts at: 4288 4344 4505 5322 6478 1790
Size: 56 161 817 1156 2573
Fragments arranged by size:
2573 1156 817 417 410 270 225 222
212 161 132 125 124 80 73 56
53 53 41 32 29
BmgBI CAC'GTC
Cuts at: 1318 3097 3415 3471 1318
Size: 1779 318 56 5108
Fragments arranged by size:
5108 1779 318 56
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2635 3004 4992
Size: 416 53 83 399 24 1218 369 1988
Cuts at: 4992 5950 442
Size: 958 1753
Fragments arranged by size:
1988 1753 1218 958 416 399 369 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2514 1056
Size: 1458 5803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2963 3107 3305 5921 1239
Size: 1724 144 198 2616 2579
Fragments arranged by size:
2616 2579 1724 198 144
Bpu10I CC'TnA_GC
Cuts at: 2030 2672 3860 3947 2030
Size: 642 1188 87 5344
Fragments arranged by size:
5344 1188 642 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5386 6251 6549 5386
Size: 865 298 6098
Fragments arranged by size:
6098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 3684 4336 5924 882
Size: 617 1032 1153 652 1588 2219
Fragments arranged by size:
2219 1588 1153 1032 652 617
BsaAI yAC'GTr
Cuts at: 493 1204 2110 2310 3016 3313 5052 493
Size: 711 906 200 706 297 1739 2702
Fragments arranged by size:
2702 1739 906 711 706 297 200
BsaBI GATnn'nnATC
Cuts at: 7255 7255
Size: 7261
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2778 4392 5068 5450
Size: 53 83 186 936 1245 1614 676 382
Cuts at: 5450 6085 275
Size: 635 1451
Fragments arranged by size:
1614 1451 1245 936 676 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5694 6092 6366 6513 6924 5694
Size: 398 274 147 411 6031
Fragments arranged by size:
6031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3278 3308 1178
Size: 30 165 30 1875 30 5131
Fragments arranged by size:
5131 1875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2191 2261 3104 1186
Size: 973 32 70 843 5343
Fragments arranged by size:
5343 973 843 70 32
BseYI C'CCAG_C
Cuts at: 2008 2113 2174 2339 2567 2761 2991 3742 3887
Size: 105 61 165 228 194 230 751 145
Cuts at: 3887 3909 4005 6464 6960 2008
Size: 22 96 2459 496 2309
Fragments arranged by size:
2459 2309 751 496 230 228 194 165
145 105 96 61 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2600 2768 2792 2828 2981 3200
Size: 573 297 567 168 24 36 153 219
Cuts at: 3200 3601 4379 4403 1163
Size: 401 778 24 4021
Fragments arranged by size:
4021 778 573 567 401 297 219 168
153 36 24 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 2592 2662 3972 4711 5472
Size: 275 186 574 17 70 1310 739 761
Cuts at: 5472 6500 1540
Size: 1028 2301
Fragments arranged by size:
2301 1310 1028 761 739 574 275 186
70 17
BsiHKAI G_wGCw'C
Cuts at: 2192 2315 2451 2838 3503 3953 4094 4167 5322
Size: 123 136 387 665 450 141 73 1155
Cuts at: 5322 5407 6478 2192
Size: 85 1071 2975
Fragments arranged by size:
2975 1155 1071 665 450 387 141 136
123 85 73
BsmI GAATG_Cn'
Cuts at: 4772 4865 4772
Size: 93 7168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2044 2068 2192 2224 2315 2436
Size: 399 225 29 24 124 32 91 121
Cuts at: 2436 2451 2568 2838 2963 2992 3395 3402 3503
Size: 15 117 270 125 29 403 7 101
Cuts at: 3503 3819 3953 4041 4094 4167 4247 4288 4344
Size: 316 134 88 53 73 80 41 56
Cuts at: 4344 4473 4505 4580 5322 5407 6478 1391
Size: 129 32 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 316 270 225
134 129 125 124 121 117 101 91
88 85 80 75 73 56 53 41
32 32 29 29 24 15 7
BspEI T'CCGG_A
Cuts at: 6924 6924
Size: 7261
BspHI T'CATG_A
Cuts at: 5150 6880 5150
Size: 1730 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2800 3411 3666 4351 4443 4458 4616 844
Size: 1956 611 255 685 92 15 158 3489
Fragments arranged by size:
3489 1956 685 611 255 158 92 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3934 5148 1394
Size: 429 2111 1214 3507
Fragments arranged by size:
3507 2111 1214 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5756 5930 65
Size: 1183 4508 174 1396
Fragments arranged by size:
4508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2462 2661 3591 3703 4014 5905 6092
Size: 110 652 199 930 112 311 1891 187
Cuts at: 6092 1700
Size: 2869
Fragments arranged by size:
2869 1891 930 652 311 199 187 112
110
BsrGI T'GTAC_A
Cuts at: 96 3294 96
Size: 3198 4063
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7261
BssSI C'ACGA_G
Cuts at: 2720 2999 5321 6333 2720
Size: 279 2322 1012 3648
Fragments arranged by size:
3648 2322 1012 279
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 3663 4144 794
Size: 2021 848 481 3911
Fragments arranged by size:
3911 2021 848 481
BstBI TT'CG_AA
Cuts at: 6065 6065
Size: 7261
BstEII G'GTnAC_C
Cuts at: 1184 2458 1184
Size: 1274 5987
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 4075 2236
Size: 1839 5422
BstYI r'GATC_y
Cuts at: 1972 2266 3456 3975 4591 4958 5343 5360 6812
Size: 294 1190 519 616 367 385 17 1452
Cuts at: 6812 6898 6910 7256 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1190 616 519 385 367 346
294 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3241 3437 4370 3241
Size: 196 933 6132
Fragments arranged by size:
6132 933 196
BtgI C'CryG_G
Cuts at: 513 1065 2241 2454 2595 2694 4149 4288 513
Size: 552 1176 213 141 99 1455 139 3486
Fragments arranged by size:
3486 1455 1176 552 213 141 139 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 2698 3595 487
Size: 683 56 1136 336 897 4153
Fragments arranged by size:
4153 1136 897 683 336 56
BtsI GCAGTG_nn'
Cuts at: 3125 3515 4165 4777 5571 5591 3125
Size: 390 650 612 794 20 4795
Fragments arranged by size:
4795 794 650 612 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7226
DraI TTT'AAA
Cuts at: 4565 4565
Size: 7261
DraIII CAC_nnn'GTG
Cuts at: 2791 3415 2791
Size: 624 6637
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6268 809
Size: 507 4952 1802
Fragments arranged by size:
4952 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2238 2328
Size: 54 1425 50 275 18 396 12 90
Cuts at: 2328 2355 2589 2631 2659 2691 2898 3036 3628
Size: 27 234 42 28 32 207 138 592
Cuts at: 3628 3880 3969 4065 4350 4708 5597 7226 8
Size: 252 89 96 285 358 889 1629 43
Fragments arranged by size:
1629 1425 889 592 396 358 285 275
252 234 207 138 96 90 89 54
50 43 42 32 28 27 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2589 2659 3969 4708 1537
Size: 275 777 70 1310 739 4090
Fragments arranged by size:
4090 1310 777 739 275 70
EarI CTCTTCn'nnn_
Cuts at: 1353 2858 3231 3962 5191 1353
Size: 1505 373 731 1229 3423
Fragments arranged by size:
3423 1505 1229 731 373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3659 5848 6222 6368 387
Size: 1064 138 2070 2189 374 146 1280
Fragments arranged by size:
2189 2070 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2449 2449
Size: 7261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 2963 3107 3212 3305 3392 3473
Size: 393 826 505 144 105 93 87 81
Cuts at: 3473 5324 5921 6708 6976 1239
Size: 1851 597 787 268 1524
Fragments arranged by size:
1851 1524 826 787 597 505 393 268
144 105 93 87 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2357 2786 2135
Size: 222 429 6610
Fragments arranged by size:
6610 429 222
EcoO109I rG'GnC_Cy
Cuts at: 2012 2064 2065 2433 2668 2725 3211 3259 3815
Size: 52 1 368 235 57 486 48 556
Cuts at: 3815 3816 4037 4038 4501 4502 2012
Size: 1 221 1 463 1 4771
Fragments arranged by size:
4771 556 486 463 368 235 221 57
52 48 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4570 4948 4570
Size: 378 6883
FspI TGC'GCA
Cuts at: 5767 5767
Size: 7261
HaeII r_GCGC'y
Cuts at: 1678 2532 3112 3451 3743 4160 4395 6408 1678
Size: 854 580 339 292 417 235 2013 2531
Fragments arranged by size:
2531 2013 854 580 417 339 292 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 3626 3658 5902
Size: 32 135 32 725 32 1297 32 2244
Cuts at: 5902 5934 5976 6008 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2244 1297 725 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4605 4851 669
Size: 988 281 2667 246 3079
Fragments arranged by size:
3079 2667 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7261
HpaI GTT'AAC
Cuts at: 4851 4851
Size: 7261
KasI G'GCGC_C
Cuts at: 4391 4391
Size: 7261
KpnI G_GTAC'C
Cuts at: 4102 4586 4102
Size: 484 6777
MfeI C'AATT_G
Cuts at: 4860 4860
Size: 7261
MluI A'CGCG_T
Cuts at: 7137 7137
Size: 7261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 6375 6559 1305
Size: 939 4131 184 2007
Fragments arranged by size:
4131 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 2330 2357 2900 3038
Size: 54 1425 343 396 102 27 543 138
Cuts at: 3038 3630 3882 4067 4352 10
Size: 592 252 185 285 2919
Fragments arranged by size:
2919 1425 592 543 396 343 285 252
185 138 102 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 4073 5219 5578 5737 518
Size: 648 1068 1839 1146 359 159 2042
Fragments arranged by size:
2042 1839 1146 1068 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2584 2597 2696 2737 2995 3191 3328 3345
Size: 978 13 99 41 258 196 137 17
Cuts at: 3345 3462 4677 5354 6502 6747 1606
Size: 117 1215 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 978 677 258 245 196
137 117 99 41 17 13
NaeI GCC'GGC
Cuts at: 2663 3705 2663
Size: 1042 6219
NarI GG'CG_CC
Cuts at: 4392 4392
Size: 7261
NcoI C'CATG_G
Cuts at: 513 1065 2454 513
Size: 552 1389 5320
Fragments arranged by size:
5320 1389 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7261
NgoMIV G'CCGG_C
Cuts at: 2661 3703 2661
Size: 1042 6219
NheI G'CTAG_C
Cuts at: 1052 2510 1052
Size: 1458 5803
NotI GC'GGCC_GC
Cuts at: 4708 4708
Size: 7261
NspI r_CATG'y
Cuts at: 1132 1229 3295 4144 4619 6164 1132
Size: 97 2066 849 475 1545 2229
Fragments arranged by size:
2229 2066 1545 849 475 97
PasI CC'CwG_GG
Cuts at: 1348 2054 2131 2638 3064 4041 4505 1348
Size: 706 77 507 426 977 464 4104
Fragments arranged by size:
4104 977 706 507 464 426 77
PciI A'CATG_T
Cuts at: 3291 6160 3291
Size: 2869 4392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2892 3415 1108
Size: 1784 523 4954
Fragments arranged by size:
4954 1784 523
PfoI T'CCnGG_A
Cuts at: 3282 3282
Size: 7261
PmeI GTTT'AAAC
Cuts at: 4565 4565
Size: 7261
PmlI CAC'GTG
Cuts at: 3313 3313
Size: 7261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 6843 6875 5330
Size: 32 1481 32 5716
Fragments arranged by size:
5716 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3259 3259
Size: 7261
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7261
PsiI TTA'TAA
Cuts at: 4831 4831
Size: 7261
PspOMI G'GGCC_C
Cuts at: 2011 2064 2432 3815 4037 4501 2011
Size: 53 368 1383 222 464 4771
Fragments arranged by size:
4771 1383 464 368 222 53
PstI C_TGCA'G
Cuts at: 830 2610 3141 3966 4613 830
Size: 1780 531 825 647 3478
Fragments arranged by size:
3478 1780 825 647 531
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7261
PvuII CAG'CTG
Cuts at: 2584 2995 3191 2584
Size: 411 196 6654
Fragments arranged by size:
6654 411 196
SacI G_AGCT'C
Cuts at: 2451 2451
Size: 7261
SacII CC_GC'GG
Cuts at: 2598 2697 2598
Size: 99 7162
SalI G'TCGA_C
Cuts at: 1936 4603 1936
Size: 2667 4594
SbfI CC_TGCA'GG
Cuts at: 3966 4613 3966
Size: 647 6614
ScaI AGT'ACT
Cuts at: 5509 7221 5509
Size: 1712 5549
SexAI A'CCwGG_T
Cuts at: 2192 2787 2925 4094 4101 2192
Size: 595 138 1169 7 5352
Fragments arranged by size:
5352 1169 595 138 7
SfcI C'TryA_G
Cuts at: 826 1045 2606 2860 3137 3962 4609 5744 6425
Size: 219 1561 254 277 825 647 1135 681
Cuts at: 6425 6616 826
Size: 191 1471
Fragments arranged by size:
1561 1471 1135 825 681 647 277 254
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2667 2906 2667
Size: 239 7022
SfoI GGC'GCC
Cuts at: 4393 4393
Size: 7261
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7261
SmaI CCC'GGG
Cuts at: 1872 2278 2534 4588 1872
Size: 406 256 2054 4545
Fragments arranged by size:
4545 2054 406 256
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5365 6266 6528 820
Size: 197 926 3422 901 262 1553
Fragments arranged by size:
3422 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5052 493
Size: 4559 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7261
SphI G_CATG'C
Cuts at: 1132 4619 1132
Size: 3487 3774
SrfI GCCC'GGGC
Cuts at: 2278 2278
Size: 7261
SspI AAT'ATT
Cuts at: 5 52 5185 5
Size: 47 5133 2081
Fragments arranged by size:
5133 2081 47
StuI AGG'CCT
Cuts at: 1517 2909 3806 1517
Size: 1392 897 4972
Fragments arranged by size:
4972 1392 897
StyI C'CwwG_G
Cuts at: 513 1065 2358 2454 2886 4516 4700 513
Size: 552 1293 96 432 1630 184 3074
Fragments arranged by size:
3074 1630 1293 552 432 184 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 2884 3370 5288 5305 5458 5643
Size: 1255 1037 267 486 1918 17 153 185
Cuts at: 5643 6088 325
Size: 445 1498
Fragments arranged by size:
1918 1498 1255 1037 486 445 267 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3294 5507 7219
Size: 274 80 33 51 1443 1317 2213 1712
Cuts at: 7219 96
Size: 138
Fragments arranged by size:
2213 1712 1443 1317 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3651 3683 7221 7253 3651
Size: 32 3538 32 3659
Fragments arranged by size:
3659 3538 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2927 1316
Size: 1611 5650
XbaI T'CTAG_A
Cuts at: 4597 4597
Size: 7261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 4156 4219 4360 2893
Size: 1263 63 141 5794
Fragments arranged by size:
5794 1263 141 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7261
XmaI C'CCGG_G
Cuts at: 1870 2276 2532 4586 1870
Size: 406 256 2054 4545
Fragments arranged by size:
4545 2054 406 256
XmnI GAAnn'nnTTC
Cuts at: 5390 5390
Size: 7261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2779 5069 276
Size: 53 83 186 936 1245 2290 2468
Fragments arranged by size:
2468 2290 1245 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SanDI SapI SwaI