(Circular) (Six-base) MAPSORT of: pFN21AE0071.seq Check: 449 from: 1 to: 6874 pFN21AE0071 6874 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2813 2921 2984 3383 3416 3444 3497 2813 Size: 108 63 399 33 28 53 6190 Fragments arranged by size: 6190 399 108 63 53 33 28 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2271 4217 1937 Size: 334 1946 4594 Fragments arranged by size: 4594 1946 334 Acc65I G'GTAC_C Cuts at: 4195 4195 Size: 6874 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2263 4937 6321 6589 1632 Size: 473 158 2674 1384 268 1917 Fragments arranged by size: 2674 1917 1384 473 268 158 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 5773 6750 1203 Size: 4570 977 1327 Fragments arranged by size: 4570 1327 977 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 5603 5603 Size: 6874 AleI CACnn'nnGTG Cuts at: 1166 2161 2258 1166 Size: 995 97 5782 Fragments arranged by size: 5782 995 97 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6874 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3038 3070 3383 3415 3038 Size: 32 313 32 6497 Fragments arranged by size: 6497 313 32 32 AlwNI CAG_nnn'CTG Cuts at: 2491 2869 3435 3559 4617 6189 2491 Size: 378 566 124 1058 1572 3176 Fragments arranged by size: 3176 1572 1058 566 378 124 ApaI G_GGCC'C Cuts at: 2661 3584 2661 Size: 923 5951 ApaLI G'TGCA_C Cuts at: 4931 6087 4931 Size: 1156 5718 ApoI r'AATT_y Cuts at: 1493 4183 4409 4561 6670 1493 Size: 2690 226 152 2109 1697 Fragments arranged by size: 2690 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5428 160 Size: 872 4396 1606 Fragments arranged by size: 4396 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 2222 2586 3041 3310 3528 3566 3577 Size: 73 279 364 455 269 218 38 11 Cuts at: 3577 4084 4199 1870 Size: 507 115 4545 Fragments arranged by size: 4545 507 455 364 279 269 218 115 73 38 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2758 2791 2953 2986 3043 3076 2758 Size: 33 162 33 57 33 6556 Fragments arranged by size: 6556 162 57 33 33 33 BamHI G'GATC_C Cuts at: 4204 4571 4204 Size: 367 6507 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2357 2457 2801 2969 Size: 325 217 627 513 57 100 344 168 Cuts at: 2969 3254 3371 4195 5650 618 Size: 285 117 824 1455 1842 Fragments arranged by size: 1842 1455 824 627 513 344 325 285 217 168 117 100 57 BanII G_rGCy'C Cuts at: 1391 2069 2661 3584 4013 4193 1391 Size: 678 592 923 429 180 4072 Fragments arranged by size: 4072 923 678 592 429 180 BbeI G_GCGC'C Cuts at: 2304 2304 Size: 6874 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5903 BbvCI CC'TCA_GC Cuts at: 3716 3716 Size: 6874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2665 2699 3022 3056 5065 5099 1903 Size: 34 728 34 323 34 2009 34 3678 Fragments arranged by size: 3678 2009 728 323 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2786 4766 5976 2786 Size: 1980 1210 3684 Fragments arranged by size: 3684 1980 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 2838 3072 Size: 107 122 71 631 605 473 693 234 Cuts at: 3072 3189 3303 3384 3417 5485 136 Size: 117 114 81 33 2068 1525 Fragments arranged by size: 2068 1525 693 631 605 473 234 122 117 114 107 81 71 33 BglII A'GATC_T Cuts at: 6869 6869 Size: 6874 BlpI GC'TnA_GC Cuts at: 4291 4291 Size: 6874 Bme1580I G_kGCm'C Cuts at: 1790 2360 2661 3374 3445 3529 3584 4935 6091 Size: 570 301 713 71 84 55 1351 1156 Cuts at: 6091 1790 Size: 2573 Fragments arranged by size: 2573 1351 1156 713 570 301 84 71 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6874 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4605 5563 442 Size: 416 53 83 399 24 3188 958 1753 Fragments arranged by size: 3188 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6874 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2584 2584 Size: 6874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 5534 1239 Size: 844 3451 2579 Fragments arranged by size: 3451 2579 844 Bpu10I CC'TnA_GC Cuts at: 3716 3716 Size: 6874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 2501 3929 4999 5864 6162 2180 Size: 321 1428 1070 865 298 2892 Fragments arranged by size: 2892 1428 1070 865 321 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3761 5537 882 Size: 617 2262 1776 2219 Fragments arranged by size: 2262 2219 1776 617 BsaAI yAC'GTr Cuts at: 493 1204 4665 493 Size: 711 3461 2702 Fragments arranged by size: 3461 2702 711 BsaBI GATnn'nnATC Cuts at: 6868 6868 Size: 6874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 2301 4681 5063 Size: 53 83 186 936 735 33 2380 382 Cuts at: 5063 5698 275 Size: 635 1451 Fragments arranged by size: 2380 1451 936 735 635 382 186 83 53 33 BsaWI w'CCGG_w Cuts at: 5307 5705 5979 6126 6537 5307 Size: 398 274 147 411 5644 Fragments arranged by size: 5644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2380 2410 2621 2651 3038 Size: 30 165 30 977 30 211 30 387 Cuts at: 3038 3068 3307 3332 3337 3362 3383 3413 1178 Size: 30 239 25 5 25 21 30 4639 Fragments arranged by size: 4639 977 387 239 211 165 30 30 30 30 30 30 25 25 21 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3340 4003 4037 4154 1186 Size: 1303 851 663 34 117 3906 Fragments arranged by size: 3906 1303 851 663 117 34 BseYI C'CCAG_C Cuts at: 2119 2263 2841 2983 3005 3583 3682 3739 3913 Size: 144 578 142 22 578 99 57 174 Cuts at: 3913 6077 6573 2119 Size: 2164 496 2420 Fragments arranged by size: 2420 2164 578 578 496 174 144 142 99 57 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 2477 2650 2758 2863 2899 3016 Size: 573 482 259 173 108 105 36 117 Cuts at: 3016 3388 3956 1163 Size: 372 568 4081 Fragments arranged by size: 4081 573 568 482 372 259 173 117 108 105 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2506 4324 5085 6113 1540 Size: 275 186 505 1818 761 1028 2301 Fragments arranged by size: 2301 1818 1028 761 505 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2069 3149 3465 4935 5020 6091 2040 Size: 29 1080 316 1470 85 1071 2823 Fragments arranged by size: 2823 1470 1080 1071 316 85 29 BsmI GAATG_Cn' Cuts at: 2735 4385 4478 2735 Size: 1650 93 5131 Fragments arranged by size: 5131 1650 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3755 3755 Size: 6874 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2069 2360 2661 3149 3374 3445 Size: 399 250 29 291 301 488 225 71 Cuts at: 3445 3465 3529 3584 4013 4193 4935 5020 6091 Size: 20 64 55 429 180 742 85 1071 Cuts at: 6091 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 488 429 399 301 291 250 225 180 85 71 64 55 29 20 BspEI T'CCGG_A Cuts at: 6537 6537 Size: 6874 BspHI T'CATG_A Cuts at: 4763 6493 4763 Size: 1730 5144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2813 2921 2984 3071 3383 3416 3444 Size: 1655 314 108 63 87 312 33 28 Cuts at: 3444 3497 4229 844 Size: 53 732 3489 Fragments arranged by size: 3489 1655 732 314 312 108 87 63 53 33 28 BsrBI CCG'CTC Cuts at: 1394 1823 4761 1394 Size: 429 2938 3507 Fragments arranged by size: 3507 2938 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5369 5543 65 Size: 1183 4121 174 1396 Fragments arranged by size: 4121 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2757 2952 3302 3732 5518 5705 1700 Size: 110 947 195 350 430 1786 187 2869 Fragments arranged by size: 2869 1786 947 430 350 195 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6874 BssHII G'CGCG_C Cuts at: 1925 2019 1925 Size: 94 6780 BssSI C'ACGA_G Cuts at: 3494 3932 4934 5946 3494 Size: 438 1002 1012 4422 Fragments arranged by size: 4422 1012 1002 438 BstAPI GCAn_nnn'nTGC Cuts at: 794 2016 2136 3435 794 Size: 1222 120 1299 4233 Fragments arranged by size: 4233 1299 1222 120 BstBI TT'CG_AA Cuts at: 2079 5678 2079 Size: 3599 3275 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6874 BstXI CCAn_nnnn'nTGG Cuts at: 3265 3636 3265 Size: 371 6503 BstYI r'GATC_y Cuts at: 1972 3037 4204 4571 4956 4973 6425 6511 6523 Size: 1065 1167 367 385 17 1452 86 12 Cuts at: 6523 6869 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1167 1065 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4015 4015 Size: 6874 BtgI C'CryG_G Cuts at: 513 1065 2004 2140 2797 3186 3868 513 Size: 552 939 136 657 389 682 3519 Fragments arranged by size: 3519 939 682 657 552 389 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6135 Fragments arranged by size: 6135 683 56 BtsI GCAGTG_nn' Cuts at: 2828 3445 4390 5184 5204 2828 Size: 617 945 794 20 4498 Fragments arranged by size: 4498 945 794 617 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6874 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4152 4187 529 Size: 35 3588 35 3216 Fragments arranged by size: 3588 3216 35 35 DraI TTT'AAA Cuts at: 4178 4178 Size: 6874 DraIII CAC_nnn'GTG Cuts at: 3996 3996 Size: 6874 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3812 3962 5881 809 Size: 507 2496 150 1919 1802 Fragments arranged by size: 2496 1919 1802 507 150 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2351 2445 2503 Size: 54 1425 50 275 18 521 94 58 Cuts at: 2503 2602 3642 3783 4321 5210 6839 8 Size: 99 1040 141 538 889 1629 43 Fragments arranged by size: 1629 1425 1040 889 538 521 275 141 99 94 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2503 4321 1537 Size: 275 691 1818 4090 Fragments arranged by size: 4090 1818 691 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3241 4804 1353 Size: 1888 1563 3423 Fragments arranged by size: 3423 1888 1563 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3875 5461 5835 5981 387 Size: 1064 138 2286 1586 374 146 1280 Fragments arranged by size: 2286 1586 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2067 2067 Size: 6874 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2105 2263 4937 5534 6321 6589 Size: 393 451 22 158 2674 597 787 268 Cuts at: 6589 1239 Size: 1524 Fragments arranged by size: 2674 1524 787 597 451 393 268 158 22 EcoNI CCTnn'n_nnAGG Cuts at: 3935 3935 Size: 6874 EcoO109I rG'GnC_Cy Cuts at: 2488 2657 2658 2848 2866 3542 3940 4032 2488 Size: 169 1 190 18 676 398 92 5330 Fragments arranged by size: 5330 676 398 190 169 92 18 1 EcoRI G'AATT_C Cuts at: 4183 4561 4183 Size: 378 6496 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3880 3913 3880 Size: 33 6841 FspI TGC'GCA Cuts at: 5380 5380 Size: 6874 HaeII r_GCGC'y Cuts at: 1678 2250 2304 3885 6021 1678 Size: 572 54 1581 2136 2531 Fragments arranged by size: 2531 2136 1581 572 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2465 2497 3332 3364 5515 Size: 32 135 32 893 32 835 32 2151 Cuts at: 5515 5547 5589 5621 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2151 893 835 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2104 2272 4218 4464 669 Size: 988 281 166 168 1946 246 3079 Fragments arranged by size: 3079 1946 988 281 246 168 166 HindIII A'AGCT_T Cuts at: 748 748 Size: 6874 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KasI G'GCGC_C Cuts at: 2300 2300 Size: 6874 KpnI G_GTAC'C Cuts at: 4199 4199 Size: 6874 MfeI C'AATT_G Cuts at: 4473 4473 Size: 6874 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5988 6172 1305 Size: 4683 184 2007 Fragments arranged by size: 4683 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2447 3785 10 Size: 54 1425 343 615 1338 3099 Fragments arranged by size: 3099 1425 1338 615 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2161 2258 3510 4832 5191 5350 518 Size: 648 995 97 1252 1322 359 159 2042 Fragments arranged by size: 2042 1322 1252 995 648 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2142 2502 3174 3180 3294 3587 3695 3917 Size: 536 360 672 6 114 293 108 222 Cuts at: 3917 4290 4967 6115 6360 1606 Size: 373 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 536 373 360 293 245 222 114 108 6 NaeI GCC'GGC Cuts at: 2954 3304 2954 Size: 350 6524 NarI GG'CG_CC Cuts at: 2301 2301 Size: 6874 NcoI C'CATG_G Cuts at: 513 1065 2004 2797 513 Size: 552 939 793 4590 Fragments arranged by size: 4590 939 793 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6874 NgoMIV G'CCGG_C Cuts at: 2952 3302 2952 Size: 350 6524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6874 NotI GC'GGCC_GC Cuts at: 2503 4321 2503 Size: 1818 5056 NspI r_CATG'y Cuts at: 1132 1229 4232 5777 1132 Size: 97 3003 1545 2229 Fragments arranged by size: 3003 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3636 4006 1348 Size: 2288 370 4216 Fragments arranged by size: 4216 2288 370 PciI A'CATG_T Cuts at: 5773 5773 Size: 6874 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6874 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3038 3070 3383 3415 4943 4975 6456 6488 3038 Size: 32 313 32 1528 32 1481 32 3424 Fragments arranged by size: 3424 1528 1481 313 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2866 3542 3940 4032 2488 Size: 378 676 398 92 5330 Fragments arranged by size: 5330 676 398 378 92 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 4444 4444 Size: 6874 PspOMI G'GGCC_C Cuts at: 2657 3580 2657 Size: 923 5951 PstI C_TGCA'G Cuts at: 830 2094 3233 3440 3726 4226 830 Size: 1264 1139 207 286 500 3478 Fragments arranged by size: 3478 1264 1139 500 286 207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6874 PvuII CAG'CTG Cuts at: 3174 3174 Size: 6874 SacI G_AGCT'C Cuts at: 2069 2069 Size: 6874 SacII CC_GC'GG Cuts at: 2143 2143 Size: 6874 SalI G'TCGA_C Cuts at: 1936 2270 4216 1936 Size: 334 1946 4594 Fragments arranged by size: 4594 1946 334 SanDI GG'GwC_CC Cuts at: 3542 4032 3542 Size: 490 6384 SapI GCTCTTCn'nnn_ Cuts at: 3241 3241 Size: 6874 SbfI CC_TGCA'GG Cuts at: 4226 4226 Size: 6874 ScaI AGT'ACT Cuts at: 2522 5122 6834 2522 Size: 2600 1712 2562 Fragments arranged by size: 2600 2562 1712 SfcI C'TryA_G Cuts at: 826 1045 2090 3229 3436 3722 4222 5357 6038 Size: 219 1045 1139 207 286 500 1135 681 Cuts at: 6038 6229 826 Size: 191 1471 Fragments arranged by size: 1471 1139 1135 1045 681 500 286 219 207 191 SfoI GGC'GCC Cuts at: 2302 2302 Size: 6874 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 2588 3579 4201 1872 Size: 716 991 622 4545 Fragments arranged by size: 4545 991 716 622 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2516 3041 3944 4978 5879 Size: 197 926 252 321 525 903 1034 901 Cuts at: 5879 6141 820 Size: 262 1553 Fragments arranged by size: 1553 1034 926 903 901 525 321 262 252 197 SnaBI TAC'GTA Cuts at: 493 4665 493 Size: 4172 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6874 SphI G_CATG'C Cuts at: 1132 4232 1132 Size: 3100 3774 SspI AAT'ATT Cuts at: 5 52 4798 5 Size: 47 4746 2081 Fragments arranged by size: 4746 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6874 StyI C'CwwG_G Cuts at: 513 1065 2004 2797 4313 513 Size: 552 939 793 1516 3074 Fragments arranged by size: 3074 1516 939 793 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3628 4901 4918 5071 5256 5701 Size: 1255 885 1163 1273 17 153 185 445 Cuts at: 5701 325 Size: 1498 Fragments arranged by size: 1498 1273 1255 1163 885 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 5120 6832 Size: 274 80 33 51 1443 543 2600 1712 Cuts at: 6832 96 Size: 138 Fragments arranged by size: 2600 1712 1443 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 2621 2653 2720 2752 3307 3339 3424 Size: 32 209 32 67 32 555 32 85 Cuts at: 3424 3456 6834 6866 2380 Size: 32 3378 32 2388 Fragments arranged by size: 3378 2388 555 209 85 67 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6874 XbaI T'CTAG_A Cuts at: 4210 4210 Size: 6874 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2612 2980 3778 2612 Size: 368 798 5708 Fragments arranged by size: 5708 798 368 XhoI C'TCGA_G Cuts at: 1943 3041 1943 Size: 1098 5776 XmaI C'CCGG_G Cuts at: 1870 2586 3577 4199 1870 Size: 716 991 622 4545 Fragments arranged by size: 4545 991 716 622 XmnI GAAnn'nnTTC Cuts at: 3395 3896 5003 3395 Size: 501 1107 5266 Fragments arranged by size: 5266 1107 501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI