Restriction map for
pFN21AE0068
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FspI HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1398 TCCGGA T^CCGGA
AcyI 982 GGCGTC G[GA]^CG[CT]C
Alw44I 1270 GTGCAC G^TGCAC
ApaI 1186 GGGCCC GGGCC^C
ApaLI 1270 GTGCAC G^TGCAC
BalI 380 TGGCCA TGG^CCA
BcgI 62 CGACGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 326 TGATCA T^GATCA
BfaI 544 CTAG C^TAG
BlpI 98 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 98 GCTCAGC GC^T[ACGT]AGC
BsaAI 1387 TACGTG [CT]AC^GT[GA]
BsaHI 982 GGCGTC G[GA]^CG[CT]C
BsaI 1402 GAGACC GGTCTC(1/5)
BsaWI 1398 TCCGGA [AT]^CCGG[AT]
BseAI 1398 TCCGGA T^CCGGA
BsmBI 240 CGTCTC CGTCTC(1/5)
BspEI 1398 TCCGGA T^CCGGA
BsrDI 275 CATTGC GCAATG(2/0)
BssHII 789 GCGCGC G^CGCGC
BssSI 280 CTCGTG CACGAG(-5/-1)
BstAPI 1044 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 98 GCTCAGC GC^T[ACGT]AGC
FbaI 326 TGATCA T^GATCA
FseI 873 GGCCGGCC GGCCGG^CC
Hin1I 982 GGCGTC G[GA]^CG[CT]C
HindIII 1462 AAGCTT A^AGCTT
MaeI 544 CTAG C^TAG
MaeII 1388 ACGT A^CGT
MaeIII 967 GTAAC ^GT[ACGT]AC
MluNI 380 TGGCCA TGG^CCA
MroI 1398 TCCGGA T^CCGGA
MscI 380 TGGCCA TGG^CCA
NspI 764 ACATGC [GA]CATG^[CT]
SmaI 779 CCCGGG CCC^GGG
XcmI 245 CCATGTTTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 779 CCCGGG C^CCGGG
XmaI 779 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 111 GTAC GT^AC
462 GTAC
AlwNI 634 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
880 CAGCAGCTG
BanII 975 GGGCTC G[GA]GC[CT]^C
1186 GGGCCC
BbsI 151 GTCTTC GAAGAC(2/6)
538 GTCTTC
BglII 551 AGATCT A^GATCT
1220 AGATCT
BpuAI 151 GTCTTC GAAGAC(2/6)
538 GTCTTC
BsaBI 999 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1377 GATTTACATC
BsgI 814 CTGCAC GTGCAG(16/14)
431 GTGCAG
BsmAI 1402 GAGAC GTCTC(1/5)
241 GTCTC
BsrBI 950 CCGCTC CCGCTC(-3/-3)
1027 GAGCGG
BstXI 356 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
458 CCATGTACCTGG
BstYI 551 AGATCT [GA]^GATC[CT]
1220 AGATCT
DraII 316 GGGACCC [GA]G^G[ACGT]CC[CT]
1121 GGGTCCC
EcoO109I 316 GGGACCC [GA]G^G[ACGT]CC[CT]
1121 GGGTCCC
HaeII 448 AGCGCC [GA]GCGC^[CT]
606 GGCGCT
HgaI 63 GACGC GACGC(5/10)
983 GCGTC
HincII 133 GTCAAC GT[CT]^[GA]AC
988 GTCAAC
HindII 133 GTCAAC GT[CT]^[GA]AC
988 GTCAAC
MamI 999 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1377 GATTTACATC
MflI 551 AGATCT [GA]^GATC[CT]
1220 AGATCT
MspA1I 634 CAGCTG C[AC]G^C[GT]G
883 CAGCTG
NaeI 874 GCCGGC GCC^GGC
1057 GCCGGC
NgoMIV 874 GCCGGC G^CCGGC
1057 GCCGGC
PpuMI 316 GGGACCC [GA]G^G[AT]CC[CT]
1121 GGGTCCC
PstI 631 CTGCAG CTGCA^G
1228 CTGCAG
PvuII 634 CAGCTG CAG^CTG
883 CAGCTG
RsaI 111 GTAC GT^AC
462 GTAC
TfiI 681 GATTC G^A[AT]TC
1358 GAATC
XhoII 551 AGATCT [GA]^GATC[CT]
1220 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI