(Circular) (Six-base) MAPSORT of: pFN21AE0068.seq Check: 3169 from: 1 to: 6241 pFN21AE0068 6241 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2545 4304 5688 5956 1632 Size: 913 1759 1384 268 1917 Fragments arranged by size: 1917 1759 1384 913 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 4970 4970 Size: 6241 AleI CACnn'nnGTG Cuts at: 1166 2621 2826 1166 Size: 1455 205 4581 Fragments arranged by size: 4581 1455 205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2844 1184 Size: 1660 4581 AlwNI CAG_nnn'CTG Cuts at: 2644 2890 3984 5556 2644 Size: 246 1094 1572 3329 Fragments arranged by size: 3329 1572 1094 246 ApaI G_GGCC'C Cuts at: 3195 3195 Size: 6241 ApaLI G'TGCA_C Cuts at: 3275 4298 5454 3275 Size: 1023 1156 4062 Fragments arranged by size: 4062 1156 1023 ApoI r'AATT_y Cuts at: 1493 3550 3776 3928 6037 1493 Size: 2057 226 152 2109 1697 Fragments arranged by size: 2109 2057 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2415 2784 3131 3566 1870 Size: 73 472 369 347 435 4545 Fragments arranged by size: 4545 472 435 369 347 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2623 3048 3335 3562 5017 Size: 325 217 627 836 425 287 227 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 836 627 425 325 287 227 217 BanII G_rGCy'C Cuts at: 1391 2984 3195 3560 1391 Size: 1593 211 365 4072 Fragments arranged by size: 4072 1593 365 211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2149 2536 928 Size: 971 250 387 4633 Fragments arranged by size: 4633 971 387 250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2056 2090 4432 4466 1903 Size: 34 119 34 2342 34 3678 Fragments arranged by size: 3678 2342 119 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 2331 1548 Size: 783 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4852 136 Size: 107 122 71 631 605 3180 1525 Fragments arranged by size: 3180 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2556 3225 6236 2556 Size: 669 3011 2561 Fragments arranged by size: 3011 2561 669 BlpI GC'TnA_GC Cuts at: 2104 3658 2104 Size: 1554 4687 Bme1580I G_kGCm'C Cuts at: 1790 2628 2833 3195 3279 4302 5458 1790 Size: 838 205 362 84 1023 1156 2573 Fragments arranged by size: 2573 1156 1023 838 362 205 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 3972 4930 Size: 416 53 83 399 24 1772 783 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 1772 1753 958 783 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2552 2783 4901 1239 Size: 806 507 231 2118 2579 Fragments arranged by size: 2579 2118 806 507 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4366 5231 5529 4366 Size: 865 298 5078 Fragments arranged by size: 5078 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3401 4904 882 Size: 617 1902 1503 2219 Fragments arranged by size: 2219 1902 1503 617 BsaAI yAC'GTr Cuts at: 493 1204 3394 4032 493 Size: 711 2190 638 2702 Fragments arranged by size: 2702 2190 711 638 BsaBI GATnn'nnATC Cuts at: 3008 3386 6235 3008 Size: 378 2849 3014 Fragments arranged by size: 3014 2849 378 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2988 4048 4430 5065 Size: 53 83 186 936 1455 1060 382 635 Cuts at: 5065 275 Size: 1451 Fragments arranged by size: 1455 1451 1060 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3403 4674 5072 5346 5493 5904 3403 Size: 1271 398 274 147 411 3740 Fragments arranged by size: 3740 1271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3502 3532 1178 Size: 30 165 30 2099 30 3887 Fragments arranged by size: 3887 2099 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2740 2846 3362 3491 1186 Size: 1554 106 516 129 3936 Fragments arranged by size: 3936 1554 516 129 106 BseYI C'CCAG_C Cuts at: 2425 3037 3500 5444 5940 2425 Size: 612 463 1944 496 2726 Fragments arranged by size: 2726 1944 612 496 463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2457 2804 1163 Size: 573 721 347 4600 Fragments arranged by size: 4600 721 573 347 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2874 3111 3279 4302 4387 5458 2874 Size: 237 168 1023 85 1071 3657 Fragments arranged by size: 3657 1071 1023 237 168 85 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 BsmBI CGTCTCn'nnnn_ Cuts at: 2251 2251 Size: 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2628 2833 2874 2984 3111 3195 3279 Size: 399 838 205 41 110 127 84 84 Cuts at: 3279 3560 4302 4387 5458 1391 Size: 281 742 85 1071 2174 Fragments arranged by size: 2174 1071 838 742 399 281 205 127 110 85 84 84 41 BspEI T'CCGG_A Cuts at: 3403 5904 3403 Size: 2501 3740 BspHI T'CATG_A Cuts at: 4130 5860 4130 Size: 1730 4511 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3596 844 Size: 2752 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2957 3034 4128 1394 Size: 429 1134 77 1094 3507 Fragments arranged by size: 3507 1134 1094 429 77 BsrDI GCAATG_nn' Cuts at: 65 1248 2279 4736 4910 65 Size: 1183 1031 2457 174 1396 Fragments arranged by size: 2457 1396 1183 1031 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2599 2879 3062 4885 5072 1700 Size: 110 789 280 183 1823 187 2869 Fragments arranged by size: 2869 1823 789 280 187 183 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 2794 1925 Size: 869 5372 BssSI C'ACGA_G Cuts at: 2285 4301 5313 2285 Size: 2016 1012 3213 Fragments arranged by size: 3213 2016 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 3980 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2368 2470 2368 Size: 102 6139 BstYI r'GATC_y Cuts at: 1972 2556 3225 3571 3938 4323 4340 5792 5878 Size: 584 669 346 367 385 17 1452 86 Cuts at: 5878 5890 6236 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 669 584 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4750 Fragments arranged by size: 4750 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3157 487 Size: 683 56 1931 3571 Fragments arranged by size: 3571 1931 683 56 BtsI GCAGTG_nn' Cuts at: 3279 3375 3757 4551 4571 3279 Size: 96 382 794 20 4949 Fragments arranged by size: 4949 794 382 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2597 2877 Size: 54 1425 50 275 18 555 212 280 Cuts at: 2877 2881 3060 3688 4577 6206 8 Size: 4 179 628 889 1629 43 Fragments arranged by size: 1629 1425 889 628 555 280 275 212 179 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4171 1353 Size: 2818 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2545 2552 2783 4304 4901 5688 Size: 393 413 500 7 231 1521 597 787 Cuts at: 5688 5956 1239 Size: 268 1524 Fragments arranged by size: 1524 1521 787 597 500 413 393 268 231 7 EcoO109I rG'GnC_Cy Cuts at: 2322 3127 2322 Size: 805 5436 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FseI GG_CCGG'CC Cuts at: 2883 2883 Size: 6241 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 2457 2615 5388 1678 Size: 779 158 2773 2531 Fragments arranged by size: 2773 2531 779 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4882 4914 4956 4988 1373 Size: 32 135 32 3310 32 42 32 2626 Fragments arranged by size: 3310 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2140 2995 3585 3831 669 Size: 988 281 202 855 590 246 3079 Fragments arranged by size: 3079 988 855 590 281 246 202 HindIII A'AGCT_T Cuts at: 748 3467 748 Size: 2719 3522 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2518 5355 5539 1305 Size: 1213 2837 184 2007 Fragments arranged by size: 2837 2007 1213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 10 Size: 54 1425 343 555 3864 Fragments arranged by size: 3864 1425 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2366 2621 2747 2826 2867 3002 3392 Size: 648 1200 255 126 79 41 135 390 Cuts at: 3392 4199 4558 4717 518 Size: 807 359 159 2042 Fragments arranged by size: 2042 1200 807 648 390 359 255 159 135 126 79 41 MspA1I CmG'CkG Cuts at: 1606 2641 2890 3657 4334 5482 5727 1606 Size: 1035 249 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 1035 767 677 249 245 NaeI GCC'GGC Cuts at: 2881 3064 2881 Size: 183 6058 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4750 Fragments arranged by size: 4750 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NgoMIV G'CCGG_C Cuts at: 2879 3062 2879 Size: 183 6058 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2773 3599 5144 1132 Size: 97 1544 826 1545 2229 Fragments arranged by size: 2229 1545 1544 826 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6241 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6241 PfoI T'CCnGG_A Cuts at: 3321 3321 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2322 3127 2322 Size: 805 5436 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6241 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspOMI G'GGCC_C Cuts at: 3191 3191 Size: 6241 PstI C_TGCA'G Cuts at: 830 2640 3237 3593 830 Size: 1810 597 356 3478 Fragments arranged by size: 3478 1810 597 356 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2641 2890 2641 Size: 249 5992 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SanDI GG'GwC_CC Cuts at: 2322 3127 2322 Size: 805 5436 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2449 2636 3233 3340 3589 4724 5405 Size: 219 1404 187 597 107 249 1135 681 Cuts at: 5405 5596 826 Size: 191 1471 Fragments arranged by size: 1471 1404 1135 681 597 249 219 191 187 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 2786 3568 1872 Size: 914 782 4545 Fragments arranged by size: 4545 914 782 SmlI C'TyrA_G Cuts at: 820 1017 1943 4345 5246 5508 820 Size: 197 926 2402 901 262 1553 Fragments arranged by size: 2402 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SrfI GCCC'GGGC Cuts at: 2786 2786 Size: 6241 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6241 StyI C'CwwG_G Cuts at: 513 1065 2004 2388 3411 3525 3680 513 Size: 552 939 384 1023 114 155 3074 Fragments arranged by size: 3074 1023 939 552 384 155 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2534 3236 4268 4285 4438 4623 Size: 1255 470 484 702 1032 17 153 185 Cuts at: 4623 5068 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1032 702 484 470 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4487 6199 96 Size: 274 80 33 51 1443 2510 1712 138 Fragments arranged by size: 2510 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3502 3534 6201 6233 3502 Size: 32 2667 32 3510 Fragments arranged by size: 3510 2667 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6241 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2257 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 2784 3566 1870 Size: 914 782 4545 Fragments arranged by size: 4545 914 782 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI