Restriction map for
pFN21AE0068
(Linear) (Six-base) MAPSORT of: KIEE0068.seq Check: 9094 from: 1 to: 1536
KIEE0068.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1536
Size: 540 996
AleI CACnn'nnGTG
Cuts at: 0 616 821 1536
Size: 616 205 715
Fragments arranged by size:
715 616 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 839 1536
Size: 839 697
AlwNI CAG_nnn'CTG
Cuts at: 0 639 885 1536
Size: 639 246 651
Fragments arranged by size:
651 639 246
ApaI G_GGCC'C
Cuts at: 0 1190 1536
Size: 1190 346
ApaLI G'TGCA_C
Cuts at: 0 1270 1536
Size: 1270 266
AvaI C'yCGr_G
Cuts at: 0 410 779 1126 1536
Size: 410 369 347 410
Fragments arranged by size:
410 410 369 347
BanI G'GyrC_C
Cuts at: 0 618 1043 1330 1536
Size: 618 425 287 206
Fragments arranged by size:
618 425 287 206
BanII G_rGCy'C
Cuts at: 0 979 1190 1536
Size: 979 211 346
Fragments arranged by size:
979 346 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 531 1536
Size: 144 387 1005
Fragments arranged by size:
1005 387 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 85 1536
Size: 51 34 1451
Fragments arranged by size:
1451 51 34
BclI T'GATC_A
Cuts at: 0 326 1536
Size: 326 1210
BglII A'GATC_T
Cuts at: 0 551 1220 1536
Size: 551 669 316
Fragments arranged by size:
669 551 316
BlpI GC'TnA_GC
Cuts at: 0 99 1536
Size: 99 1437
Bme1580I G_kGCm'C
Cuts at: 0 623 828 1190 1274 1536
Size: 623 205 362 84 262
Fragments arranged by size:
623 362 262 205 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 1184 1536
Size: 1184 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 547 778 1536
Size: 40 507 231 758
Fragments arranged by size:
758 507 231 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 1396 1536
Size: 1396 140
BsaAI yAC'GTr
Cuts at: 0 1389 1536
Size: 1389 147
BsaBI GATnn'nnATC
Cuts at: 0 1003 1381 1536
Size: 1003 378 155
Fragments arranged by size:
1003 378 155
BsaHI Gr'CG_yC
Cuts at: 0 983 1536
Size: 983 553
BsaWI w'CCGG_w
Cuts at: 0 1398 1536
Size: 1398 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1497 1527 1536
Size: 1497 30 9
Fragments arranged by size:
1497 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 735 841 1357 1486 1536
Size: 735 106 516 129 50
Fragments arranged by size:
735 516 129 106 50
BseYI C'CCAG_C
Cuts at: 0 420 1032 1495 1536
Size: 420 612 463 41
Fragments arranged by size:
612 463 420 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 799 1536
Size: 452 347 737
Fragments arranged by size:
737 452 347
BsiHKAI G_wGCw'C
Cuts at: 0 869 1106 1274 1536
Size: 869 237 168 262
Fragments arranged by size:
869 262 237 168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 246 1536
Size: 246 1290
Bsp1286I G_dGCh'C
Cuts at: 0 623 828 869 979 1106 1190 1274 1536
Size: 623 205 41 110 127 84 84 262
Fragments arranged by size:
623 262 205 127 110 84 84 41
BspEI T'CCGG_A
Cuts at: 0 1398 1536
Size: 1398 138
BsrBI CCG'CTC
Cuts at: 0 952 1029 1536
Size: 952 77 507
Fragments arranged by size:
952 507 77
BsrDI GCAATG_nn'
Cuts at: 0 274 1536
Size: 274 1262
BsrFI r'CCGG_y
Cuts at: 0 594 874 1057 1536
Size: 594 280 183 479
Fragments arranged by size:
594 479 280 183
BssHII G'CGCG_C
Cuts at: 0 789 1536
Size: 789 747
BssSI C'ACGA_G
Cuts at: 0 280 1536
Size: 280 1256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1536
Size: 1050 486
BstXI CCAn_nnnn'nTGG
Cuts at: 0 363 465 1536
Size: 363 102 1071
Fragments arranged by size:
1071 363 102
BstYI r'GATC_y
Cuts at: 0 551 1220 1536
Size: 551 669 316
Fragments arranged by size:
669 551 316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1152 1536
Size: 1152 384
BtsI GCAGTG_nn'
Cuts at: 0 1274 1370 1536
Size: 1274 96 166
Fragments arranged by size:
1274 166 96
EaeI y'GGCC_r
Cuts at: 0 380 592 872 876 1055 1536
Size: 380 212 280 4 179 481
Fragments arranged by size:
481 380 280 212 179 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 540 547 778 1536
Size: 40 500 7 231 758
Fragments arranged by size:
758 500 231 40 7
EcoO109I rG'GnC_Cy
Cuts at: 0 317 1122 1536
Size: 317 805 414
Fragments arranged by size:
805 414 317
FseI GG_CCGG'CC
Cuts at: 0 878 1536
Size: 878 658
HaeII r_GCGC'y
Cuts at: 0 452 610 1536
Size: 452 158 926
Fragments arranged by size:
926 452 158
HincII GTy'rAC
Cuts at: 0 135 990 1536
Size: 135 855 546
Fragments arranged by size:
855 546 135
HindIII A'AGCT_T
Cuts at: 0 1462 1536
Size: 1462 74
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1536
Size: 513 1023
MscI TGG'CCA
Cuts at: 0 382 1536
Size: 382 1154
MslI CAynn'nnrTG
Cuts at: 0 361 616 742 821 862 997 1387 1536
Size: 361 255 126 79 41 135 390 149
Fragments arranged by size:
390 361 255 149 135 126 79 41
MspA1I CmG'CkG
Cuts at: 0 636 885 1536
Size: 636 249 651
Fragments arranged by size:
651 636 249
NaeI GCC'GGC
Cuts at: 0 876 1059 1536
Size: 876 183 477
Fragments arranged by size:
876 477 183
NgoMIV G'CCGG_C
Cuts at: 0 874 1057 1536
Size: 874 183 479
Fragments arranged by size:
874 479 183
NspI r_CATG'y
Cuts at: 0 768 1536
Size: 768 768
PfoI T'CCnGG_A
Cuts at: 0 1316 1536
Size: 1316 220
PpuMI rG'GwC_Cy
Cuts at: 0 317 1122 1536
Size: 317 805 414
Fragments arranged by size:
805 414 317
PspOMI G'GGCC_C
Cuts at: 0 1186 1536
Size: 1186 350
PstI C_TGCA'G
Cuts at: 0 635 1232 1536
Size: 635 597 304
Fragments arranged by size:
635 597 304
PvuII CAG'CTG
Cuts at: 0 636 885 1536
Size: 636 249 651
Fragments arranged by size:
651 636 249
SanDI GG'GwC_CC
Cuts at: 0 317 1122 1536
Size: 317 805 414
Fragments arranged by size:
805 414 317
SfcI C'TryA_G
Cuts at: 0 444 631 1228 1335 1536
Size: 444 187 597 107 201
Fragments arranged by size:
597 444 201 187 107
SmaI CCC'GGG
Cuts at: 0 781 1536
Size: 781 755
SrfI GCCC'GGGC
Cuts at: 0 781 1536
Size: 781 755
StyI C'CwwG_G
Cuts at: 0 383 1406 1520 1536
Size: 383 1023 114 16
Fragments arranged by size:
1023 383 114 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 529 1231 1536
Size: 45 484 702 305
Fragments arranged by size:
702 484 305 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1497 1529 1536
Size: 1497 32 7
Fragments arranged by size:
1497 32 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 1536
Size: 252 1284
XmaI C'CCGG_G
Cuts at: 0 779 1536
Size: 779 757
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BcgI BclI BglII BlpI Bme1580I BmrI
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgZI BtsI
EaeI Eco57MI EcoO109I FseI HaeII HincII HindIII MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PfoI PpuMI
PspOMI PstI PvuII SanDI SfcI SmaI SrfI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BmgBI
BmtI BplI Bpu10I BpuEI BsiEI BsiWI BsmI BspHI
BspMI BsrGI BstBI BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FspI FspAI Hin4I HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI