(Circular) (Six-base) MAPSORT of: pFN21AE0067.seq Check: 7173 from: 1 to: 9046 pFN21AE0067 9046 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3367 3367 Size: 9046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4631 6856 278 Size: 53 83 186 936 3095 2225 2468 Fragments arranged by size: 3095 2468 2225 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 4136 6389 1937 Size: 990 1209 2253 4594 Fragments arranged by size: 4594 2253 1209 990 Acc65I G'GTAC_C Cuts at: 2032 4044 6367 2032 Size: 2012 2323 4711 Fragments arranged by size: 4711 2323 2012 AclI AA'CG_TT Cuts at: 1563 7173 7546 1563 Size: 5610 373 3063 Fragments arranged by size: 5610 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3443 3500 5104 5364 6234 7109 8493 8761 Size: 1811 57 1604 260 870 875 1384 268 Cuts at: 8761 1632 Size: 1917 Fragments arranged by size: 1917 1811 1604 1384 875 870 268 260 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8849 AflIII A'CryG_T Cuts at: 1203 7945 8922 1203 Size: 6742 977 1327 Fragments arranged by size: 6742 1327 977 AgeI A'CCGG_T Cuts at: 2093 7877 2093 Size: 5784 3262 AhdI GACnn_n'nnGTC Cuts at: 7775 7775 Size: 9046 AleI CACnn'nnGTG Cuts at: 1166 3704 1166 Size: 2538 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6339 1184 Size: 5155 3891 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4404 4436 6069 6101 4404 Size: 32 1633 32 7349 Fragments arranged by size: 7349 1633 32 32 AlwNI CAG_nnn'CTG Cuts at: 2447 3068 6212 6789 8361 2447 Size: 621 3144 577 1572 3132 Fragments arranged by size: 3144 3132 1572 621 577 ApaI G_GGCC'C Cuts at: 6120 6120 Size: 9046 ApaLI G'TGCA_C Cuts at: 3240 7103 8259 3240 Size: 3863 1156 4027 Fragments arranged by size: 4027 3863 1156 ApoI r'AATT_y Cuts at: 1493 2628 2691 3773 3981 4754 5067 5080 6355 Size: 1135 63 1082 208 773 313 13 1275 Cuts at: 6355 6581 6733 8842 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1275 1135 1082 773 313 226 208 152 63 13 AseI AT'TA_AT Cuts at: 160 1032 7600 160 Size: 872 6568 1606 Fragments arranged by size: 6568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9046 AvaI C'yCGr_G Cuts at: 1870 1943 2565 5694 6371 1870 Size: 73 622 3129 677 4545 Fragments arranged by size: 4545 3129 677 622 73 BamHI G'GATC_C Cuts at: 5466 6376 6743 5466 Size: 910 367 7769 Fragments arranged by size: 7769 910 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 3351 4044 5761 5980 Size: 325 217 627 245 1319 693 1717 219 Cuts at: 5980 6367 7822 618 Size: 387 1455 1842 Fragments arranged by size: 1842 1717 1455 1319 693 627 387 325 245 219 217 BanII G_rGCy'C Cuts at: 1391 2505 2979 4304 5651 5692 6120 6365 1391 Size: 1114 474 1325 1347 41 428 245 4072 Fragments arranged by size: 4072 1347 1325 1114 474 428 245 41 BbeI G_GCGC'C Cuts at: 3355 3355 Size: 9046 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3782 4704 928 Size: 971 1883 922 5270 Fragments arranged by size: 5270 1883 971 922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7237 7271 1903 Size: 34 5300 34 3678 Fragments arranged by size: 5300 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3156 6938 8148 3156 Size: 3782 1210 4054 Fragments arranged by size: 4054 3782 1210 BclI T'GATC_A Cuts at: 1548 2217 4183 5304 1548 Size: 669 1966 1121 5290 Fragments arranged by size: 5290 1966 1121 669 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5696 7657 136 Size: 107 122 71 631 605 4024 1961 1525 Fragments arranged by size: 4024 1961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4519 9041 4519 Size: 4522 4524 BlpI GC'TnA_GC Cuts at: 2641 6337 6463 2641 Size: 3696 126 5224 Fragments arranged by size: 5224 3696 126 Bme1580I G_kGCm'C Cuts at: 1790 3084 3244 3877 5782 6120 7107 8263 1790 Size: 1294 160 633 1905 338 987 1156 2573 Fragments arranged by size: 2573 1905 1294 1156 987 633 338 160 BmgBI CAC'GTC Cuts at: 1318 3865 1318 Size: 2547 6499 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4466 5606 5719 Size: 416 53 83 399 24 3049 1140 113 Cuts at: 5719 5776 6777 7735 442 Size: 57 1001 958 1753 Fragments arranged by size: 3049 1753 1140 1001 958 416 399 113 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4257 4257 Size: 9046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2607 3262 3388 4046 4082 4134 5684 7706 Size: 1368 655 126 658 36 52 1550 2022 Cuts at: 7706 1239 Size: 2579 Fragments arranged by size: 2579 2022 1550 1368 658 655 126 52 36 Bpu10I CC'TnA_GC Cuts at: 3258 5958 3258 Size: 2700 6346 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 2647 4158 7171 8036 8334 2465 Size: 182 1511 3013 865 298 3177 Fragments arranged by size: 3177 3013 1511 865 298 182 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2295 7709 882 Size: 617 796 5414 2219 Fragments arranged by size: 5414 2219 796 617 BsaAI yAC'GTr Cuts at: 493 1204 4821 6837 493 Size: 711 3617 2016 2702 Fragments arranged by size: 3617 2702 2016 711 BsaBI GATnn'nnATC Cuts at: 4482 9040 4482 Size: 4558 4488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3825 4628 6853 Size: 53 83 186 936 1819 473 803 2225 Cuts at: 6853 7235 7870 275 Size: 382 635 1451 Fragments arranged by size: 2225 1819 1451 936 803 635 473 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2093 4653 7479 7877 8151 8298 8709 2093 Size: 2560 2826 398 274 147 411 2430 Fragments arranged by size: 2826 2560 2430 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2157 2187 2199 2229 2457 Size: 30 165 30 754 30 12 30 228 Cuts at: 2457 2487 3099 3129 4404 4434 4828 4858 1178 Size: 30 612 30 1275 30 394 30 5366 Fragments arranged by size: 5366 1275 754 612 394 228 165 30 30 30 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2220 3930 4233 5268 5990 6239 1186 Size: 982 52 1710 303 1035 722 249 3993 Fragments arranged by size: 3993 1710 1035 982 722 303 249 52 BseYI C'CCAG_C Cuts at: 4593 5385 8249 8745 4593 Size: 792 2864 496 4894 Fragments arranged by size: 4894 2864 792 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3682 4791 5664 1163 Size: 573 1946 1109 873 4545 Fragments arranged by size: 4545 1946 1109 873 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 6496 7257 8285 1540 Size: 275 186 202 4293 761 1028 2301 Fragments arranged by size: 4293 2301 1028 761 275 202 186 BsiHKAI G_wGCw'C Cuts at: 2979 3244 5651 7107 7192 8263 2979 Size: 265 2407 1456 85 1071 3762 Fragments arranged by size: 3762 2407 1456 1071 265 85 BsmI GAATG_Cn' Cuts at: 2238 5962 6557 6650 2238 Size: 3724 595 93 4634 Fragments arranged by size: 4634 3724 595 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3347 3832 3895 5808 3347 Size: 485 63 1913 6585 Fragments arranged by size: 6585 1913 485 63 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2505 2979 3084 3244 3877 4304 5651 Size: 399 715 474 105 160 633 427 1347 Cuts at: 5651 5692 5782 6120 6365 7107 7192 8263 1391 Size: 41 90 338 245 742 85 1071 2174 Fragments arranged by size: 2174 1347 1071 742 715 633 474 427 399 338 245 160 105 90 85 41 BspEI T'CCGG_A Cuts at: 8709 8709 Size: 9046 BspHI T'CATG_A Cuts at: 4230 5473 5706 6309 6935 8665 4230 Size: 1243 233 603 626 1730 4611 Fragments arranged by size: 4611 1730 1243 626 603 233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3367 3612 5304 6401 844 Size: 2523 245 1692 1097 3489 Fragments arranged by size: 3489 2523 1692 1097 245 BsrBI CCG'CTC Cuts at: 1394 1823 6933 1394 Size: 429 5110 3507 Fragments arranged by size: 5110 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2591 7541 7715 65 Size: 1183 1343 4950 174 1396 Fragments arranged by size: 4950 1396 1343 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 3971 7690 7877 1700 Size: 110 283 1878 3719 187 2869 Fragments arranged by size: 3719 2869 1878 283 187 110 BsrGI T'GTAC_A Cuts at: 96 3059 3495 4552 96 Size: 2963 436 1057 4590 Fragments arranged by size: 4590 2963 1057 436 BssHII G'CGCG_C Cuts at: 1925 2076 1925 Size: 151 8895 BssSI C'ACGA_G Cuts at: 4884 7106 8118 4884 Size: 2222 1012 5812 Fragments arranged by size: 5812 2222 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 794 Size: 1611 7435 BstBI TT'CG_AA Cuts at: 5065 7850 5065 Size: 2785 6261 BstEII G'GTnAC_C Cuts at: 1184 5314 1184 Size: 4130 4916 BstXI CCAn_nnnn'nTGG Cuts at: 3307 3559 5417 3307 Size: 252 1858 6936 Fragments arranged by size: 6936 1858 252 BstYI r'GATC_y Cuts at: 1972 2701 4519 5035 5466 6176 6376 6743 7128 Size: 729 1818 516 431 710 200 367 385 Cuts at: 7128 7145 8597 8683 8695 9041 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1818 1452 729 710 516 431 385 367 346 200 86 17 12 BstZ17I GTA'TAC Cuts at: 2928 4137 2928 Size: 1209 7837 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2996 5715 513 Size: 552 939 186 806 2719 3844 Fragments arranged by size: 3844 2719 939 806 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8307 Fragments arranged by size: 8307 683 56 BtsI GCAGTG_nn' Cuts at: 2120 5275 6562 7356 7376 2120 Size: 3155 1287 794 20 3790 Fragments arranged by size: 3790 3155 1287 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4245 4280 5073 5108 529 Size: 35 3681 35 793 35 4467 Fragments arranged by size: 4467 3681 793 35 35 35 DraI TTT'AAA Cuts at: 3006 6350 3006 Size: 3344 5702 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4607 4859 8053 809 Size: 507 3291 252 3194 1802 Fragments arranged by size: 3291 3194 1802 507 252 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3561 5192 6493 Size: 54 1425 50 275 18 1731 1631 1301 Cuts at: 6493 7382 9011 8 Size: 889 1629 43 Fragments arranged by size: 1731 1631 1629 1425 1301 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6493 1537 Size: 275 4681 4090 Fragments arranged by size: 4681 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 4203 5123 5383 6129 6976 1353 Size: 707 2143 920 260 746 847 3423 Fragments arranged by size: 3423 2143 920 847 746 707 260 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2982 7633 8007 8153 387 Size: 1064 138 1393 4651 374 146 1280 Fragments arranged by size: 4651 1393 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2977 5649 2977 Size: 2672 6374 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2607 3262 3388 3443 3500 4046 4082 Size: 393 975 655 126 55 57 546 36 Cuts at: 4082 4134 5104 5364 5684 6234 7109 7706 8493 Size: 52 970 260 320 550 875 597 787 Cuts at: 8493 8761 1239 Size: 268 1524 Fragments arranged by size: 1524 975 970 875 787 655 597 550 546 393 320 268 260 126 57 55 52 36 EcoNI CCTnn'n_nnAGG Cuts at: 3945 3945 Size: 9046 EcoO109I rG'GnC_Cy Cuts at: 3453 4209 5443 6116 3453 Size: 756 1234 673 6383 Fragments arranged by size: 6383 1234 756 673 EcoRI G'AATT_C Cuts at: 4754 5067 6355 6733 4754 Size: 313 1288 378 7067 Fragments arranged by size: 7067 1288 378 313 EcoRV GAT'ATC Cuts at: 5819 5819 Size: 9046 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6198 6198 Size: 9046 FspI TGC'GCA Cuts at: 7552 7552 Size: 9046 HaeII r_GCGC'y Cuts at: 1678 3355 5754 8193 1678 Size: 1677 2399 2439 2531 Fragments arranged by size: 2531 2439 2399 1677 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 2363 2395 2559 Size: 32 135 32 585 32 174 32 164 Cuts at: 2559 2591 3099 3131 3294 3326 3731 3763 4691 Size: 32 508 32 163 32 405 32 928 Cuts at: 4691 4723 5711 5743 7687 7719 7761 7793 1373 Size: 32 988 32 1944 32 42 32 2626 Fragments arranged by size: 2626 1944 988 928 585 508 405 174 164 163 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6390 6636 669 Size: 988 281 4452 246 3079 Fragments arranged by size: 4452 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2109 4395 748 Size: 1361 2286 5399 Fragments arranged by size: 5399 2286 1361 HpaI GTT'AAC Cuts at: 6636 6636 Size: 9046 KasI G'GCGC_C Cuts at: 3351 3351 Size: 9046 KpnI G_GTAC'C Cuts at: 2036 4048 6371 2036 Size: 2012 2323 4711 Fragments arranged by size: 4711 2323 2012 MfeI C'AATT_G Cuts at: 4977 5241 6645 4977 Size: 264 1404 7378 Fragments arranged by size: 7378 1404 264 MluI A'CGCG_T Cuts at: 8922 8922 Size: 9046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2223 2372 2635 2869 3042 4152 4217 5473 Size: 918 149 263 234 173 1110 65 1256 Cuts at: 5473 5754 8160 8344 1305 Size: 281 2406 184 2007 Fragments arranged by size: 2406 2007 1256 1110 918 281 263 234 184 173 149 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 3563 5194 10 Size: 54 1425 343 1731 1631 3862 Fragments arranged by size: 3862 1731 1631 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3557 3704 4229 4265 4532 5178 5268 Size: 648 2391 147 525 36 267 646 90 Cuts at: 5268 5333 5759 7004 7363 7522 518 Size: 65 426 1245 359 159 2042 Fragments arranged by size: 2391 2042 1245 648 646 525 426 359 267 159 147 90 65 36 MspA1I CmG'CkG Cuts at: 1606 2192 2722 3348 4593 6462 7139 8287 8532 Size: 586 530 626 1245 1869 677 1148 245 Cuts at: 8532 1606 Size: 2120 Fragments arranged by size: 2120 1869 1245 1148 677 626 586 530 245 NarI GG'CG_CC Cuts at: 3352 3352 Size: 9046 NcoI C'CATG_G Cuts at: 513 1065 2004 2996 5715 513 Size: 552 939 992 2719 3844 Fragments arranged by size: 3844 2719 992 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9046 NotI GC'GGCC_GC Cuts at: 6493 6493 Size: 9046 NspI r_CATG'y Cuts at: 1132 1229 4814 6404 7949 1132 Size: 97 3585 1590 1545 2229 Fragments arranged by size: 3585 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9046 PciI A'CATG_T Cuts at: 7945 7945 Size: 9046 PflMI CCAn_nnn'nTGG Cuts at: 1108 4794 1108 Size: 3686 5360 PfoI T'CCnGG_A Cuts at: 2704 2778 2704 Size: 74 8972 PmeI GTTT'AAAC Cuts at: 6350 6350 Size: 9046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4404 4436 7115 7147 8628 8660 4404 Size: 32 2679 32 1481 32 4790 Fragments arranged by size: 4790 2679 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3453 5443 3453 Size: 1990 7056 PshAI GACnn'nnGTC Cuts at: 1613 5166 1613 Size: 3553 5493 PsiI TTA'TAA Cuts at: 3753 3807 6616 3753 Size: 54 2809 6183 Fragments arranged by size: 6183 2809 54 PspOMI G'GGCC_C Cuts at: 6116 6116 Size: 9046 PstI C_TGCA'G Cuts at: 830 2974 3364 4144 5527 6398 830 Size: 2144 390 780 1383 871 3478 Fragments arranged by size: 3478 2144 1383 871 780 390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9046 PvuII CAG'CTG Cuts at: 2722 3348 4593 2722 Size: 626 1245 7175 Fragments arranged by size: 7175 1245 626 SacI G_AGCT'C Cuts at: 2979 5651 2979 Size: 2672 6374 SacII CC_GC'GG Cuts at: 2193 2193 Size: 9046 SalI G'TCGA_C Cuts at: 1936 6388 1936 Size: 4452 4594 SanDI GG'GwC_CC Cuts at: 3453 3453 Size: 9046 SapI GCTCTTCn'nnn_ Cuts at: 5383 5383 Size: 9046 SbfI CC_TGCA'GG Cuts at: 5527 6398 5527 Size: 871 8175 ScaI AGT'ACT Cuts at: 4792 7294 9006 4792 Size: 2502 1712 4832 Fragments arranged by size: 4832 2502 1712 SexAI A'CCwGG_T Cuts at: 2819 4445 2819 Size: 1626 7420 SfcI C'TryA_G Cuts at: 826 1045 2970 3360 4140 5523 6394 7529 8210 Size: 219 1925 390 780 1383 871 1135 681 Cuts at: 8210 8401 826 Size: 191 1471 Fragments arranged by size: 1925 1471 1383 1135 871 780 681 390 219 191 SfoI GGC'GCC Cuts at: 3353 3353 Size: 9046 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9046 SmaI CCC'GGG Cuts at: 1872 5696 6373 1872 Size: 3824 677 4545 Fragments arranged by size: 4545 3824 677 SmlI C'TyrA_G Cuts at: 820 1017 1943 2480 2662 4173 7150 8051 8313 Size: 197 926 537 182 1511 2977 901 262 Cuts at: 8313 820 Size: 1553 Fragments arranged by size: 2977 1553 1511 926 901 537 262 197 182 SnaBI TAC'GTA Cuts at: 493 6837 493 Size: 6344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9046 SphI G_CATG'C Cuts at: 1132 6404 1132 Size: 5272 3774 SspI AAT'ATT Cuts at: 5 52 2959 5497 6970 5 Size: 47 2907 2538 1473 2081 Fragments arranged by size: 2907 2538 2081 1473 47 StuI AGG'CCT Cuts at: 1517 3765 4731 1517 Size: 2248 966 5832 Fragments arranged by size: 5832 2248 966 StyI C'CwwG_G Cuts at: 513 1065 2004 2250 2996 3679 4848 4914 4959 Size: 552 939 246 746 683 1169 66 45 Cuts at: 4959 5164 5715 6154 6485 513 Size: 205 551 439 331 3074 Fragments arranged by size: 3074 1169 939 746 683 552 551 439 331 246 205 66 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2217 2864 4501 4750 5423 5889 6039 Size: 1255 637 647 1637 249 673 466 150 Cuts at: 6039 7073 7090 7243 7428 7873 325 Size: 1034 17 153 185 445 1498 Fragments arranged by size: 1637 1498 1255 1034 673 647 637 466 445 249 185 153 150 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2050 3059 3495 Size: 274 80 33 51 1443 73 1009 436 Cuts at: 3495 4077 4552 4790 7292 9004 96 Size: 582 475 238 2502 1712 138 Fragments arranged by size: 2502 1712 1443 1009 582 475 436 274 238 138 80 73 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2112 2144 2199 2231 2412 2444 2457 2489 3063 Size: 32 55 32 181 32 13 32 574 Cuts at: 3063 3095 4828 4860 4870 4902 9006 9038 2112 Size: 32 1733 32 10 32 4104 32 2120 Fragments arranged by size: 4104 2120 1733 574 181 55 32 32 32 32 32 32 32 32 13 10 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9046 XbaI T'CTAG_A Cuts at: 4162 6382 4162 Size: 2220 6826 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2220 2517 2817 4038 4128 4983 5171 2220 Size: 297 300 1221 90 855 188 6095 Fragments arranged by size: 6095 1221 855 300 297 188 90 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9046 XmaI C'CCGG_G Cuts at: 1870 5694 6371 1870 Size: 3824 677 4545 Fragments arranged by size: 4545 3824 677 XmnI GAAnn'nnTTC Cuts at: 3773 4419 7175 3773 Size: 646 2756 5644 Fragments arranged by size: 5644 2756 646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4629 6854 276 Size: 53 83 186 936 3095 2225 2468 Fragments arranged by size: 3095 2468 2225 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI