Restriction map for
pFN21AE0064
(Circular) (Six-base) MAPSORT of: pFN21AE0064.seq Check: 9047 from: 1 to: 6442
pFN21AE0064 6442 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 6442
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3785 1937
Size: 1848 4594
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2889 4505 5889 6157 1632
Size: 1257 1616 1384 268 1917
Fragments arranged by size:
1917 1616 1384 1257 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 3121 5341 6318 1203
Size: 1918 2220 977 1327
Fragments arranged by size:
2220 1918 1327 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 2216 2282 3028 1166
Size: 1050 66 746 4580
Fragments arranged by size:
4580 1050 746 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2867 1184
Size: 1683 4759
AlwNI CAG_nnn'CTG
Cuts at: 2652 2928 4185 5757 2652
Size: 276 1257 1572 3337
Fragments arranged by size:
3337 1572 1257 276
ApaI G_GGCC'C
Cuts at: 3515 3515
Size: 6442
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 3751 3977 4129 6238 1493
Size: 2258 226 152 2109 1697
Fragments arranged by size:
2258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
AvrII C'CTAG_G
Cuts at: 3541 3541
Size: 6442
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3095 3451 3763 5218 618
Size: 325 217 627 1308 356 312 1455 1842
Fragments arranged by size:
1842 1455 1308 627 356 325 312 217
BanII G_rGCy'C
Cuts at: 1391 2720 2835 3474 3515 3761 1391
Size: 1329 115 639 41 246 4072
Fragments arranged by size:
4072 1329 639 246 115 41
BbeI G_GCGC'C
Cuts at: 3099 3455 3099
Size: 356 6086
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5471
BbvCI CC'TCA_GC
Cuts at: 2481 3113 2481
Size: 632 5810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2732 2766 4633 4667 1903
Size: 34 795 34 1867 34 3678
Fragments arranged by size:
3678 1867 795 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3312 4334 5544 3312
Size: 1022 1210 4210
Fragments arranged by size:
4210 1210 1022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BfrBI ATG'CAT
Cuts at: 3243 3243
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2700 3515 4503 5659 1790
Size: 910 815 988 1156 2573
Fragments arranged by size:
2573 1156 988 910 815
BmgBI CAC'GTC
Cuts at: 1318 2966 3134 3218 3224 3386 1318
Size: 1648 168 84 6 162 4374
Fragments arranged by size:
4374 1648 168 162 84 6
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 2444 2539
Size: 416 53 83 399 24 739 288 95
Cuts at: 2539 2578 2617 2695 4173 5131 442
Size: 39 39 78 1478 958 1753
Fragments arranged by size:
1753 1478 958 739 416 399 288 95
83 78 53 39 39 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 3010 2854
Size: 156 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 2824 3729 5102 1239
Size: 1158 427 905 1373 2579
Fragments arranged by size:
2579 1373 1158 905 427
Bpu10I CC'TnA_GC
Cuts at: 2481 3108 3113 2481
Size: 627 5 5810
Fragments arranged by size:
5810 627 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3531 3681 5105 882
Size: 617 2032 150 1424 2219
Fragments arranged by size:
2219 2032 1424 617 150
BsaAI yAC'GTr
Cuts at: 493 1204 2362 3124 4233 493
Size: 711 1158 762 1109 2702
Fragments arranged by size:
2702 1158 1109 762 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2506 3058 3096 3163
Size: 53 83 186 936 973 552 38 67
Cuts at: 3163 3452 4249 4631 5266 275
Size: 289 797 382 635 1451
Fragments arranged by size:
1451 973 936 797 635 552 382 289
186 83 67 53 38
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3123 3153 3568 3598 1178
Size: 30 165 30 1720 30 415 30 4022
Fragments arranged by size:
4022 1720 415 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 2469 2766 2769 1186
Size: 882 401 297 3 4859
Fragments arranged by size:
4859 882 401 297 3
BseYI C'CCAG_C
Cuts at: 3497 3507 5645 6141 3497
Size: 10 2138 496 3798
Fragments arranged by size:
3798 2138 496 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 2872 1163
Size: 573 648 488 4733
Fragments arranged by size:
4733 648 573 488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2413 2759 3411 4503 4588 5659 2413
Size: 346 652 1092 85 1071 3196
Fragments arranged by size:
3196 1092 1071 652 346 85
BsmI GAATG_Cn'
Cuts at: 2450 3953 4046 2450
Size: 1503 93 4846
Fragments arranged by size:
4846 1503 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2956 2956
Size: 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2413 2700 2720 2759 2835 3411 3474
Size: 399 623 287 20 39 76 576 63
Cuts at: 3474 3515 3761 4503 4588 5659 1391
Size: 41 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 623 576 399 287 246
85 76 63 41 39 20
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 3550 4331 6061 3550
Size: 781 1730 3931
Fragments arranged by size:
3931 1730 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2612 2859 3004 3797 844
Size: 1389 379 247 145 793 3489
Fragments arranged by size:
3489 1389 793 379 247 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 2748 2904 3063 3664 4329 1394
Size: 429 218 707 156 159 601 665 3507
Fragments arranged by size:
3507 707 665 601 429 218 159 156
BsrDI GCAATG_nn'
Cuts at: 65 1248 3703 4937 5111 65
Size: 1183 2455 1234 174 1396
Fragments arranged by size:
2455 1396 1234 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3448 5086 5273 1700
Size: 110 1638 1638 187 2869
Fragments arranged by size:
2869 1638 1638 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3731 794
Size: 2937 3505
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 3074 3074
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2022 3772 4139 4524 4541 5993 6079 6091
Size: 50 1750 367 385 17 1452 86 12
Cuts at: 6091 6437 1972
Size: 346 1977
Fragments arranged by size:
1977 1750 1452 385 367 346 86 50
17 12
Bsu36I CC'TnA_GG
Cuts at: 2526 2526
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 3158 3335 3682 513
Size: 552 2093 177 347 3273
Fragments arranged by size:
3273 2093 552 347 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3211 487
Size: 683 56 1985 3718
Fragments arranged by size:
3718 1985 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 2520 3188 3371 3958 4752 4772 2372
Size: 148 668 183 587 794 20 4042
Fragments arranged by size:
4042 794 668 587 183 148 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DraIII CAC_nnn'GTG
Cuts at: 2547 2547
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3338 3679 3889
Size: 54 1425 50 275 18 1508 341 210
Cuts at: 3889 4778 6407 8
Size: 889 1629 43
Fragments arranged by size:
1629 1508 1425 889 341 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4372 1353
Size: 3019 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2397 2824 2889 3729 4505 5102 5889
Size: 393 765 427 65 840 776 597 787
Cuts at: 5889 6157 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 776 765 597 427 393
268 65
EcoNI CCTnn'n_nnAGG
Cuts at: 3357 3357
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2063 3511 2063
Size: 1448 4994
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 3099 3455 5589 1678
Size: 1421 356 2134 2531
Fragments arranged by size:
2531 2134 1421 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5083 5115 5157 5189 1373
Size: 32 135 32 3511 32 42 32 2626
Fragments arranged by size:
3511 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3145 748
Size: 2397 4045
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KasI G'GCGC_C
Cuts at: 3095 3451 3095
Size: 356 6086
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2068 3353 5556 5740 1305
Size: 763 1285 2203 184 2007
Fragments arranged by size:
2203 2007 1285 763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3681 10
Size: 54 1425 343 1849 2771
Fragments arranged by size:
2771 1849 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2282 3028 3388 4400 4759 4918
Size: 648 1050 66 746 360 1012 359 159
Cuts at: 4918 518
Size: 2042
Fragments arranged by size:
2042 1050 1012 746 648 360 359 159
66
MspA1I CmG'CkG
Cuts at: 1606 2652 2808 3337 3858 4535 5683 5928 1606
Size: 1046 156 529 521 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 529 521 245 156
NaeI GCC'GGC
Cuts at: 3450 3450
Size: 6442
NarI GG'CG_CC
Cuts at: 3096 3452 3096
Size: 356 6086
NcoI C'CATG_G
Cuts at: 513 1065 3682 513
Size: 552 2617 3273
Fragments arranged by size:
3273 2617 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 3448 3448
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 3245 3245
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 3800 5345 1132
Size: 97 2571 1545 2229
Fragments arranged by size:
2571 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2713 3359 1348
Size: 1365 646 4431
Fragments arranged by size:
4431 1365 646
PciI A'CATG_T
Cuts at: 5341 5341
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 1108
Size: 1002 5440
PfoI T'CCnGG_A
Cuts at: 2672 2672
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2063 2063
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 3174 1613
Size: 1561 4881
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 3511 3511
Size: 6442
PstI C_TGCA'G
Cuts at: 830 2515 2793 3195 3399 3479 3794 830
Size: 1685 278 402 204 80 315 3478
Fragments arranged by size:
3478 1685 402 315 278 204 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2652 2808 2652
Size: 156 6286
SacII CC_GC'GG
Cuts at: 3338 3338
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SanDI GG'GwC_CC
Cuts at: 2063 2063
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3479 3794 3479
Size: 315 6127
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2511 2566 2644 2789 3191 3395 3475
Size: 219 1466 55 78 145 402 204 80
Cuts at: 3475 3790 4925 5606 5797 826
Size: 315 1135 681 191 1471
Fragments arranged by size:
1471 1466 1135 681 402 315 219 204
191 145 80 78 55
SfoI GGC'GCC
Cuts at: 3097 3453 3097
Size: 356 6086
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 2638 1517
Size: 1121 5321
StyI C'CwwG_G
Cuts at: 513 1065 3541 3682 3691 3881 513
Size: 552 2476 141 9 190 3074
Fragments arranged by size:
3074 2476 552 190 141 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 2558 2658 3426 4469 4486 4639
Size: 1255 714 264 100 768 1043 17 153
Cuts at: 4639 4824 5269 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1043 768 714 445 264 185
153 100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4688 6400 96
Size: 274 80 33 51 1443 2711 1712 138
Fragments arranged by size:
2711 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 3568 3600 6402 6434 3123
Size: 32 413 32 2802 32 3131
Fragments arranged by size:
3131 2802 413 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3250 3778 3250
Size: 528 5914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2720 2720
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 3312 4571 3312
Size: 1259 5183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BstZ17I EcoICRI EcoRV
FalI FseI FspAI NruI PacI PmlI PspXI PsrI
RsrII SacI SapI SexAI SfiI SrfI SwaI