Restriction map for
pFN21AE0064
(Linear) (Six-base) MAPSORT of: KIEE0064.seq Check: 1477 from: 1 to: 1737
KIEE0064.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 1737
Size: 228 1509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1737
Size: 884 853
AflIII A'CryG_T
Cuts at: 0 1116 1737
Size: 1116 621
AleI CACnn'nnGTG
Cuts at: 0 211 277 1023 1737
Size: 211 66 746 714
Fragments arranged by size:
746 714 211 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 862 1737
Size: 862 875
AlwNI CAG_nnn'CTG
Cuts at: 0 647 923 1737
Size: 647 276 814
Fragments arranged by size:
814 647 276
ApaI G_GGCC'C
Cuts at: 0 1510 1737
Size: 1510 227
AvrII C'CTAG_G
Cuts at: 0 1536 1737
Size: 1536 201
BanI G'GyrC_C
Cuts at: 0 1090 1446 1737
Size: 1090 356 291
Fragments arranged by size:
1090 356 291
BanII G_rGCy'C
Cuts at: 0 715 830 1469 1510 1737
Size: 715 115 639 41 227
Fragments arranged by size:
715 639 227 115 41
BbeI G_GCGC'C
Cuts at: 0 1094 1450 1737
Size: 1094 356 287
Fragments arranged by size:
1094 356 287
BbvCI CC'TCA_GC
Cuts at: 0 476 1108 1737
Size: 476 632 629
Fragments arranged by size:
632 629 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 727 761 1737
Size: 727 34 976
Fragments arranged by size:
976 727 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1307 1737
Size: 1307 430
BfrBI ATG'CAT
Cuts at: 0 1238 1737
Size: 1238 499
Bme1580I G_kGCm'C
Cuts at: 0 695 1510 1737
Size: 695 815 227
Fragments arranged by size:
815 695 227
BmgBI CAC'GTC
Cuts at: 0 961 1129 1213 1219 1381 1737
Size: 961 168 84 6 162 356
Fragments arranged by size:
961 356 168 162 84 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 439 534 573 612 690 1737
Size: 151 288 95 39 39 78 1047
Fragments arranged by size:
1047 288 151 95 78 39 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 849 1005 1737
Size: 849 156 732
Fragments arranged by size:
849 732 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 819 1724 1737
Size: 392 427 905 13
Fragments arranged by size:
905 427 392 13
Bpu10I CC'TnA_GC
Cuts at: 0 476 1103 1108 1737
Size: 476 627 5 629
Fragments arranged by size:
629 627 476 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 1526 1676 1737
Size: 1526 150 61
Fragments arranged by size:
1526 150 61
BsaAI yAC'GTr
Cuts at: 0 357 1119 1737
Size: 357 762 618
Fragments arranged by size:
762 618 357
BsaHI Gr'CG_yC
Cuts at: 0 501 1053 1091 1158 1447 1737
Size: 501 552 38 67 289 290
Fragments arranged by size:
552 501 290 289 67 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1118 1148 1563 1593 1737
Size: 1118 30 415 30 144
Fragments arranged by size:
1118 415 144 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 464 761 764 1737
Size: 63 401 297 3 973
Fragments arranged by size:
973 401 297 63 3
BseYI C'CCAG_C
Cuts at: 0 1492 1502 1737
Size: 1492 10 235
Fragments arranged by size:
1492 235 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 867 1737
Size: 379 488 870
Fragments arranged by size:
870 488 379
BsiHKAI G_wGCw'C
Cuts at: 0 408 754 1406 1737
Size: 408 346 652 331
Fragments arranged by size:
652 408 346 331
BsmI GAATG_Cn'
Cuts at: 0 445 1737
Size: 445 1292
BsmBI CGTCTCn'nnnn_
Cuts at: 0 951 1737
Size: 951 786
Bsp1286I G_dGCh'C
Cuts at: 0 408 695 715 754 830 1406 1469 1510
Size: 408 287 20 39 76 576 63 41
Cuts at: 1510 1737
Size: 227
Fragments arranged by size:
576 408 287 227 76 63 41 39
20
BspHI T'CATG_A
Cuts at: 0 1545 1737
Size: 1545 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 607 854 999 1737
Size: 228 379 247 145 738
Fragments arranged by size:
738 379 247 228 145
BsrBI CCG'CTC
Cuts at: 0 36 743 899 1058 1659 1737
Size: 36 707 156 159 601 78
Fragments arranged by size:
707 601 159 156 78 36
BsrDI GCAATG_nn'
Cuts at: 0 1698 1737
Size: 1698 39
BsrFI r'CCGG_y
Cuts at: 0 1443 1737
Size: 1443 294
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1726 1737
Size: 1726 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1069 1737
Size: 1069 668
BstYI r'GATC_y
Cuts at: 0 17 1737
Size: 17 1720
Bsu36I CC'TnA_GG
Cuts at: 0 521 1737
Size: 521 1216
BtgI C'CryG_G
Cuts at: 0 1153 1330 1677 1737
Size: 1153 177 347 60
Fragments arranged by size:
1153 347 177 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1206 1737
Size: 1206 531
BtsI GCAGTG_nn'
Cuts at: 0 367 515 1183 1366 1737
Size: 367 148 668 183 371
Fragments arranged by size:
668 371 367 183 148
DraIII CAC_nnn'GTG
Cuts at: 0 542 1737
Size: 542 1195
EaeI y'GGCC_r
Cuts at: 0 1333 1674 1737
Size: 1333 341 63
Fragments arranged by size:
1333 341 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 819 884 1724 1737
Size: 392 427 65 840 13
Fragments arranged by size:
840 427 392 65 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1352 1737
Size: 1352 385
EcoO109I rG'GnC_Cy
Cuts at: 0 58 1506 1737
Size: 58 1448 231
Fragments arranged by size:
1448 231 58
HaeII r_GCGC'y
Cuts at: 0 1094 1450 1737
Size: 1094 356 287
Fragments arranged by size:
1094 356 287
HindIII A'AGCT_T
Cuts at: 0 1140 1737
Size: 1140 597
KasI G'GCGC_C
Cuts at: 0 1090 1446 1737
Size: 1090 356 291
Fragments arranged by size:
1090 356 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1348 1737
Size: 63 1285 389
Fragments arranged by size:
1285 389 63
MscI TGG'CCA
Cuts at: 0 1676 1737
Size: 1676 61
MslI CAynn'nnrTG
Cuts at: 0 211 277 1023 1383 1737
Size: 211 66 746 360 354
Fragments arranged by size:
746 360 354 211 66
MspA1I CmG'CkG
Cuts at: 0 647 803 1332 1737
Size: 647 156 529 405
Fragments arranged by size:
647 529 405 156
NaeI GCC'GGC
Cuts at: 0 1445 1737
Size: 1445 292
NarI GG'CG_CC
Cuts at: 0 1091 1447 1737
Size: 1091 356 290
Fragments arranged by size:
1091 356 290
NcoI C'CATG_G
Cuts at: 0 1677 1737
Size: 1677 60
NgoMIV G'CCGG_C
Cuts at: 0 1443 1737
Size: 1443 294
NsiI A_TGCA'T
Cuts at: 0 1240 1737
Size: 1240 497
PasI CC'CwG_GG
Cuts at: 0 708 1354 1737
Size: 708 646 383
Fragments arranged by size:
708 646 383
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1737
Size: 105 1632
PfoI T'CCnGG_A
Cuts at: 0 667 1737
Size: 667 1070
PpuMI rG'GwC_Cy
Cuts at: 0 58 1737
Size: 58 1679
PshAI GACnn'nnGTC
Cuts at: 0 1169 1737
Size: 1169 568
PspOMI G'GGCC_C
Cuts at: 0 1506 1737
Size: 1506 231
PstI C_TGCA'G
Cuts at: 0 510 788 1190 1394 1474 1737
Size: 510 278 402 204 80 263
Fragments arranged by size:
510 402 278 263 204 80
PvuII CAG'CTG
Cuts at: 0 647 803 1737
Size: 647 156 934
Fragments arranged by size:
934 647 156
SacII CC_GC'GG
Cuts at: 0 1333 1737
Size: 1333 404
SanDI GG'GwC_CC
Cuts at: 0 58 1737
Size: 58 1679
SbfI CC_TGCA'GG
Cuts at: 0 1474 1737
Size: 1474 263
SfcI C'TryA_G
Cuts at: 0 506 561 639 784 1186 1390 1470 1737
Size: 506 55 78 145 402 204 80 267
Fragments arranged by size:
506 402 267 204 145 80 78 55
SfoI GGC'GCC
Cuts at: 0 1092 1448 1737
Size: 1092 356 289
Fragments arranged by size:
1092 356 289
StuI AGG'CCT
Cuts at: 0 633 1737
Size: 633 1104
StyI C'CwwG_G
Cuts at: 0 1536 1677 1686 1737
Size: 1536 141 9 51
Fragments arranged by size:
1536 141 51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 289 553 653 1421 1737
Size: 289 264 100 768 316
Fragments arranged by size:
768 316 289 264 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1563 1595 1737
Size: 1118 32 413 32 142
Fragments arranged by size:
1118 413 142 32 32
XbaI T'CTAG_A
Cuts at: 0 1245 1737
Size: 1245 492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 715 1737
Size: 715 1022
XmnI GAAnn'nnTTC
Cuts at: 0 1307 1737
Size: 1307 430
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvrII
BanI BanII BbeI BbvCI BcgI BciVI BfrBI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI Eco57MI EcoNI
EcoO109I HaeII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI PasI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuII SacII SanDI SbfI
SfcI SfoI StuI StyI TaqII TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvaI BaeI
BamHI BbsI BclI BglI BglII BlpI BmtI BpuEI
BsaBI BsaWI BsiEI BsiWI BspEI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NspI PacI PciI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XhoI XmaI ZraI