Restriction map for
pFN21AE0061
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AhdI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BglI
BglII BlnI BpuAI BsaBI BsiWI
BspDI BspHI BspLU11I BstAPI BstBI
ClaI CpoI DraIII DrdI Eam1105I
EarI EcoT14I EcoT22I FseI FspI
HindIII HpaI Ksp632I MamI MfeI
MunI NcoI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 1096 GGTACC G^GTACC
AccIII 615 TCCGGA T^CCGGA
AclI 760 AACGTT AA^CGTT
AflII 891 CTTAAG C^TTAAG
AgeI 1836 ACCGGT A^CCGGT
AlwNI 148 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2329 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1231 AGCGCT AGC^GCT
ApaI 2286 GGGCCC GGGCC^C
Asp700I 414 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1096 GGTACC G^GTACC
BalI 2476 TGGCCA TGG^CCA
BbeI 1698 GGCGCC GGCGC^C
BfrI 891 CTTAAG C^TTAAG
BlpI 2168 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2168 GCTCAGC GC^T[ACGT]AGC
BseAI 615 TCCGGA T^CCGGA
BsmBI 1915 GAGACG CGTCTC(1/5)
BsmI 2438 GCATTC GAATGC(1/-1)
BspEI 615 TCCGGA T^CCGGA
BsrBI 1539 GAGCGG CCGCTC(-3/-3)
BsrDI 2055 CATTGC GCAATG(2/0)
Bsu36I 2329 CCTCAGG CC^T[ACGT]AGG
CelII 2168 GCTCAGC GC^T[ACGT]AGC
DraI 2566 TTTAAA TTT^AAA
EagI 1844 CGGCCG C^GGCCG
EclXI 1844 CGGCCG C^GGCCG
Eco47III 1231 AGCGCT AGC^GCT
Eco52I 1844 CGGCCG C^GGCCG
Eco81I 2329 CCTCAGG CC^T[ACGT]AGG
EcoRI 258 GAATTC G^AATTC
EcoRV 481 GATATC GAT^ATC
KasI 1698 GGCGCC G^GCGCC
KpnI 1096 GGTACC GGTAC^C
KspI 1744 CCGCGG CCGC^GG
MluI 1811 ACGCGT A^CGCGT
MluNI 2476 TGGCCA TGG^CCA
MroI 615 TCCGGA T^CCGGA
MscI 2476 TGGCCA TGG^CCA
NaeI 1686 GCCGGC GCC^GGC
NarI 1698 GGCGCC GG^CGCC
NdeI 919 CATATG CA^TATG
NgoMIV 1686 GCCGGC G^CCGGC
NheI 1315 GCTAGC G^CTAGC
PflMI 1764 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1836 ACCGGT A^CCGGT
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 760 AACGTT AA^CGTT
PvuII 2556 CAGCTG CAG^CTG
SacII 1744 CCGCGG CCGC^GG
SexAI 1092 ACCAGGT A^CC[AT]GGT
SfoI 1698 GGCGCC GGC^GCC
SmlI 891 CTTAAG C^T[CT][GA]AG
SphI 2405 GCATGC GCATG^C
Van91I 1764 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 872 CCAAGTTCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 414 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 738 GTATAC GT^[AC][GT]AC
1377 GTATAC
Alw44I 1528 GTGCAC G^TGCAC
2419 GTGCAC
ApaLI 1528 GTGCAC G^TGCAC
2419 GTGCAC
BciVI 16 GGATAC GTATCC(6/5)
2485 GTATCC
BclI 2099 TGATCA T^GATCA
2466 TGATCA
BsaAI 1519 TACGTG [CT]AC^GT[GA]
1781 TACGTG
BsaI 2068 GAGACC GGTCTC(1/5)
2722 GAGACC
BsgI 303 GTGCAG GTGCAG(16/14)
1784 GTGCAG
BsiEI 270 CGACCG CG[GA][CT]^CG
1844 CGGCCG
BssSI 1573 CACGAG CACGAG(-5/-1)
1901 CACGAG
Bst1107I 738 GTATAC GTA^TAC
1377 GTATAC
BstEII 1152 GGTTACC G^GT[ACGT]ACC
1272 GGTTACC
BstPI 1152 GGTTACC G^GT[ACGT]ACC
1272 GGTTACC
BstZ17I 738 GTATAC GTA^TAC
1377 GTATAC
EcoNI 2325 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2424 CCTAGAGGAGG
EcoO65I 1152 GGTTACC G^GT[ACGT]ACC
1272 GGTTACC
FbaI 2099 TGATCA T^GATCA
2466 TGATCA
MslI 787 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2211 CACTCTGGTG
NspI 2191 GCATGT [GA]CATG^[CT]
2405 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII