(Circular) (Six-base) MAPSORT of: pFN21AE0061.seq Check: 3093 from: 1 to: 7516 pFN21AE0061 7516 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3649 3649 Size: 7516 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5326 278 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 3383 4859 1937 Size: 807 639 1476 4594 Fragments arranged by size: 4594 1476 807 639 Acc65I G'GTAC_C Cuts at: 3101 4837 3101 Size: 1736 5780 AclI AA'CG_TT Cuts at: 1563 2766 5643 6016 1563 Size: 1203 2877 373 3063 Fragments arranged by size: 3063 2877 1203 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4303 5579 6963 7231 1632 Size: 2671 1276 1384 268 1917 Fragments arranged by size: 2671 1917 1384 1276 268 AfeI AGC'GCT Cuts at: 3238 3238 Size: 7516 AflII C'TTAA_G Cuts at: 820 1017 2896 820 Size: 197 1879 5440 Fragments arranged by size: 5440 1879 197 AflIII A'CryG_T Cuts at: 1203 3525 3816 4095 6415 7392 1203 Size: 2322 291 279 2320 977 1327 Fragments arranged by size: 2322 2320 1327 977 291 279 AgeI A'CCGG_T Cuts at: 3841 6347 3841 Size: 2506 5010 AhdI GACnn_n'nnGTC Cuts at: 6245 6245 Size: 7516 AleI CACnn'nnGTG Cuts at: 1166 4220 1166 Size: 3054 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7516 AlwNI CAG_nnn'CTG Cuts at: 2158 5259 6831 2158 Size: 3101 1572 2843 Fragments arranged by size: 3101 2843 1572 ApaI G_GGCC'C Cuts at: 4295 4295 Size: 7516 ApaLI G'TGCA_C Cuts at: 3533 4424 5573 6729 3533 Size: 891 1149 1156 4320 Fragments arranged by size: 4320 1156 1149 891 ApoI r'AATT_y Cuts at: 1493 2210 2263 4625 4825 5051 5203 7312 1493 Size: 717 53 2362 200 226 152 2109 1697 Fragments arranged by size: 2362 2109 1697 717 226 200 152 53 AseI AT'TA_AT Cuts at: 160 1032 6070 160 Size: 872 5038 1606 Fragments arranged by size: 5038 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2663 3377 3708 4841 1870 Size: 73 168 552 714 331 1133 4545 Fragments arranged by size: 4545 1133 714 552 331 168 73 BamHI G'GATC_C Cuts at: 4846 5213 4846 Size: 367 7149 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 2675 3101 3703 4027 Size: 325 217 627 395 493 426 602 324 Cuts at: 4027 4837 6292 618 Size: 810 1455 1842 Fragments arranged by size: 1842 1455 810 627 602 493 426 395 325 324 217 BanII G_rGCy'C Cuts at: 1391 2525 3299 3913 3975 4295 4835 1391 Size: 1134 774 614 62 320 540 4072 Fragments arranged by size: 4072 1134 774 614 540 320 62 BbeI G_GCGC'C Cuts at: 3707 3707 Size: 7516 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5707 5741 1903 Size: 34 3770 34 3678 Fragments arranged by size: 3770 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2015 4501 5408 6618 2015 Size: 2486 907 1210 2913 Fragments arranged by size: 2913 2486 1210 907 BclI T'GATC_A Cuts at: 1548 4104 4471 1548 Size: 2556 367 4593 Fragments arranged by size: 4593 2556 367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6127 136 Size: 107 122 71 631 605 4455 1525 Fragments arranged by size: 4455 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7511 7511 Size: 7516 BlpI GC'TnA_GC Cuts at: 4174 4933 4174 Size: 759 6757 Bme1580I G_kGCm'C Cuts at: 1790 2015 3537 4295 4428 5577 6733 1790 Size: 225 1522 758 133 1149 1156 2573 Fragments arranged by size: 2573 1522 1156 1149 758 225 133 BmgBI CAC'GTC Cuts at: 1318 2365 2548 3346 1318 Size: 1047 183 798 5488 Fragments arranged by size: 5488 1047 798 183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2717 2954 5247 Size: 416 53 83 399 24 1300 237 2293 Cuts at: 5247 6205 442 Size: 958 1753 Fragments arranged by size: 2293 1753 1300 958 416 399 237 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3324 1056 Size: 2268 5248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2111 Size: 7516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2249 2342 2833 2847 3608 4213 4518 Size: 947 63 93 491 14 761 605 305 Cuts at: 4518 6176 1239 Size: 1658 2579 Fragments arranged by size: 2579 1658 947 761 605 491 305 93 63 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5641 6506 6804 5641 Size: 865 298 6353 Fragments arranged by size: 6353 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4067 4721 6179 882 Size: 617 2568 654 1458 2219 Fragments arranged by size: 2568 2219 1458 654 617 BsaAI yAC'GTr Cuts at: 493 1204 3526 3788 5307 493 Size: 711 2322 262 1519 2702 Fragments arranged by size: 2702 2322 1519 711 262 BsaBI GATnn'nnATC Cuts at: 7510 7510 Size: 7516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3090 3173 3704 4037 Size: 53 83 186 936 1557 83 531 333 Cuts at: 4037 5323 5705 6340 275 Size: 1286 382 635 1451 Fragments arranged by size: 1557 1451 1286 936 635 531 382 333 186 83 83 53 BsaWI w'CCGG_w Cuts at: 2395 2620 3841 4601 5949 6347 6621 6768 7179 Size: 225 1221 760 1348 398 274 147 411 Cuts at: 7179 2395 Size: 2732 Fragments arranged by size: 2732 1348 1221 760 411 398 274 225 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2831 2861 4379 4409 1178 Size: 30 165 30 1428 30 1518 30 4285 Fragments arranged by size: 4285 1518 1428 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2297 3596 4448 4739 1186 Size: 943 168 1299 852 291 3963 Fragments arranged by size: 3963 1299 943 852 291 168 BseYI C'CCAG_C Cuts at: 2404 4257 4325 6719 7215 2404 Size: 1853 68 2394 496 2705 Fragments arranged by size: 2705 2394 1853 496 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 3810 1163 Size: 573 593 1481 4869 Fragments arranged by size: 4869 1481 593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2278 3852 4966 5727 6755 1540 Size: 275 186 277 1574 1114 761 1028 2301 Fragments arranged by size: 2301 1574 1114 1028 761 277 275 186 BsiHKAI G_wGCw'C Cuts at: 2950 3537 4175 4229 4283 4428 5577 5662 6733 Size: 587 638 54 54 145 1149 85 1071 Cuts at: 6733 2950 Size: 3733 Fragments arranged by size: 3733 1149 1071 638 587 145 85 54 54 BsmI GAATG_Cn' Cuts at: 4443 5027 5120 4443 Size: 584 93 6839 Fragments arranged by size: 6839 584 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3914 3914 Size: 7516 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2525 2950 3299 3537 3913 3975 Size: 399 225 510 425 349 238 376 62 Cuts at: 3975 4175 4229 4283 4295 4428 4835 5577 5662 Size: 200 54 54 12 133 407 742 85 Cuts at: 5662 6733 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 510 425 407 399 376 349 238 225 200 133 85 62 54 54 12 BspEI T'CCGG_A Cuts at: 2620 7179 2620 Size: 4559 2957 BspHI T'CATG_A Cuts at: 5405 7135 5405 Size: 1730 5786 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2338 2557 3170 3649 4871 844 Size: 1494 219 613 479 1222 3489 Fragments arranged by size: 3489 1494 1222 613 479 219 BsrBI CCG'CTC Cuts at: 1394 1823 3546 5403 1394 Size: 429 1723 1857 3507 Fragments arranged by size: 3507 1857 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4059 6011 6185 65 Size: 1183 2811 1952 174 1396 Fragments arranged by size: 2811 1952 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3691 3841 3851 6160 6347 1700 Size: 110 1881 150 10 2309 187 2869 Fragments arranged by size: 2869 2309 1881 187 150 110 10 BsrGI T'GTAC_A Cuts at: 96 3005 3882 4155 96 Size: 2909 877 273 3457 Fragments arranged by size: 3457 2909 877 273 BssHII G'CGCG_C Cuts at: 1925 3508 3510 3612 3614 1925 Size: 1583 2 102 2 5827 Fragments arranged by size: 5827 1583 102 2 2 BssSI C'ACGA_G Cuts at: 3578 3906 5576 6588 3578 Size: 328 1670 1012 4506 Fragments arranged by size: 4506 1670 1012 328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7516 BstBI TT'CG_AA Cuts at: 6320 6320 Size: 7516 BstEII G'GTnAC_C Cuts at: 1184 3157 3277 1184 Size: 1973 120 5423 Fragments arranged by size: 5423 1973 120 BstXI CCAn_nnnn'nTGG Cuts at: 4000 4135 4237 4000 Size: 135 102 7279 Fragments arranged by size: 7279 135 102 BstYI r'GATC_y Cuts at: 1972 2221 2955 4640 4846 5213 5598 5615 7067 Size: 249 734 1685 206 367 385 17 1452 Cuts at: 7067 7153 7165 7511 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1685 1452 734 385 367 346 249 206 86 17 12 BstZ17I GTA'TAC Cuts at: 2745 3384 2745 Size: 639 6877 Bsu36I CC'TnA_GG Cuts at: 4335 4335 Size: 7516 BtgI C'CryG_G Cuts at: 513 1065 2004 2215 3330 3749 3964 513 Size: 552 939 211 1115 419 215 4065 Fragments arranged by size: 4065 1115 939 552 419 215 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2131 2413 3633 4093 487 Size: 683 56 905 282 1220 460 3910 Fragments arranged by size: 3910 1220 905 683 460 282 56 BtsI GCAGTG_nn' Cuts at: 3315 3695 3738 4484 5032 5826 5846 3315 Size: 380 43 746 548 794 20 4985 Fragments arranged by size: 4985 794 746 548 380 43 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7516 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2197 2232 529 Size: 35 1633 35 5813 Fragments arranged by size: 5813 1633 35 35 DraI TTT'AAA Cuts at: 4573 4820 4573 Size: 247 7269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6523 809 Size: 507 5207 1802 Fragments arranged by size: 5207 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2643 3339 3849 Size: 54 1425 50 275 18 813 696 510 Cuts at: 3849 4021 4481 4963 5852 7481 8 Size: 172 460 482 889 1629 43 Fragments arranged by size: 1629 1425 889 813 696 510 482 460 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3849 4963 1537 Size: 275 2037 1114 4090 Fragments arranged by size: 4090 2037 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5446 1353 Size: 4093 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2189 2311 3250 6103 6477 6623 Size: 1064 138 600 122 939 2853 374 146 Cuts at: 6623 387 Size: 1280 Fragments arranged by size: 2853 1280 1064 939 600 374 146 138 122 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2249 2342 2833 2847 3608 4213 Size: 393 554 63 93 491 14 761 605 Cuts at: 4213 4303 4518 5579 6176 6963 7231 1239 Size: 90 215 1061 597 787 268 1524 Fragments arranged by size: 1524 1061 787 761 605 597 554 491 393 268 215 93 90 63 14 EcoNI CCTnn'n_nnAGG Cuts at: 4334 4433 4334 Size: 99 7417 EcoO109I rG'GnC_Cy Cuts at: 2155 4291 4466 2155 Size: 2136 175 5205 Fragments arranged by size: 5205 2136 175 EcoRI G'AATT_C Cuts at: 2263 4825 5203 2263 Size: 2562 378 4576 Fragments arranged by size: 4576 2562 378 EcoRV GAT'ATC Cuts at: 2488 2488 Size: 7516 FspI TGC'GCA Cuts at: 6022 6022 Size: 7516 HaeII r_GCGC'y Cuts at: 1678 3240 3707 4181 6663 1678 Size: 1562 467 474 2482 2531 Fragments arranged by size: 2531 2482 1562 474 467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2858 2890 3242 Size: 32 135 32 887 32 367 32 352 Cuts at: 3242 3274 3281 3313 3788 3820 6157 6189 6231 Size: 32 7 32 475 32 2337 32 42 Cuts at: 6231 6263 1373 Size: 32 2626 Fragments arranged by size: 2626 2337 887 475 367 352 135 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3148 3796 4093 4552 4860 Size: 988 281 1116 94 648 297 459 308 Cuts at: 4860 5106 669 Size: 246 3079 Fragments arranged by size: 3079 1116 988 648 459 308 297 281 246 94 HindIII A'AGCT_T Cuts at: 748 748 Size: 7516 HpaI GTT'AAC Cuts at: 5106 5106 Size: 7516 KasI G'GCGC_C Cuts at: 3703 3703 Size: 7516 KpnI G_GTAC'C Cuts at: 3105 4841 3105 Size: 1736 5780 MfeI C'AATT_G Cuts at: 5115 5115 Size: 7516 MluI A'CGCG_T Cuts at: 3816 7392 3816 Size: 3576 3940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3552 6630 6814 1305 Size: 2247 3078 184 2007 Fragments arranged by size: 3078 2247 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4483 10 Size: 54 1425 343 2651 3043 Fragments arranged by size: 3043 2651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2796 4220 5474 5833 5992 518 Size: 648 1630 1424 1254 359 159 2042 Fragments arranged by size: 2042 1630 1424 1254 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 3751 4563 4932 5609 6757 7002 1606 Size: 738 1407 812 369 677 1148 245 2120 Fragments arranged by size: 2120 1407 1148 812 738 677 369 245 NaeI GCC'GGC Cuts at: 3693 3693 Size: 7516 NarI GG'CG_CC Cuts at: 3704 3704 Size: 7516 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6025 Fragments arranged by size: 6025 939 552 NdeI CA'TA_TG Cuts at: 387 2925 387 Size: 2538 4978 NgoMIV G'CCGG_C Cuts at: 3691 3691 Size: 7516 NheI G'CTAG_C Cuts at: 1052 3320 1052 Size: 2268 5248 NotI GC'GGCC_GC Cuts at: 4963 4963 Size: 7516 NspI r_CATG'y Cuts at: 1132 1229 4200 4414 4874 6419 1132 Size: 97 2971 214 460 1545 2229 Fragments arranged by size: 2971 2229 1545 460 214 97 PasI CC'CwG_GG Cuts at: 1348 2030 1348 Size: 682 6834 PciI A'CATG_T Cuts at: 6415 6415 Size: 7516 PflMI CCAn_nnn'nTGG Cuts at: 1108 3775 1108 Size: 2667 4849 PfoI T'CCnGG_A Cuts at: 2541 4459 2541 Size: 1918 5598 PmeI GTTT'AAAC Cuts at: 4820 4820 Size: 7516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 7098 7130 5585 Size: 32 1481 32 5971 Fragments arranged by size: 5971 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2155 Size: 7516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7516 PsiI TTA'TAA Cuts at: 2060 5086 2060 Size: 3026 4490 PspOMI G'GGCC_C Cuts at: 4291 4291 Size: 7516 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2728 2760 3374 3406 2728 Size: 32 614 32 6838 Fragments arranged by size: 6838 614 32 32 PstI C_TGCA'G Cuts at: 830 2244 2349 3567 3660 4868 830 Size: 1414 105 1218 93 1208 3478 Fragments arranged by size: 3478 1414 1218 1208 105 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7516 PvuII CAG'CTG Cuts at: 4563 4563 Size: 7516 SacII CC_GC'GG Cuts at: 3752 3752 Size: 7516 SalI G'TCGA_C Cuts at: 1936 4858 1936 Size: 2922 4594 SbfI CC_TGCA'GG Cuts at: 4868 4868 Size: 7516 ScaI AGT'ACT Cuts at: 5764 7476 5764 Size: 1712 5804 SexAI A'CCwGG_T Cuts at: 3097 4812 3097 Size: 1715 5801 SfcI C'TryA_G Cuts at: 826 1045 2240 2345 3563 3656 4350 4864 5999 Size: 219 1195 105 1218 93 694 514 1135 Cuts at: 5999 6680 6871 826 Size: 681 191 1471 Fragments arranged by size: 1471 1218 1195 1135 694 681 514 219 191 105 93 SfoI GGC'GCC Cuts at: 3705 3705 Size: 7516 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7516 SmaI CCC'GGG Cuts at: 1872 4843 1872 Size: 2971 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2896 5620 6521 6783 820 Size: 197 926 953 2724 901 262 1553 Fragments arranged by size: 2724 1553 953 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5307 493 Size: 4814 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7516 SphI G_CATG'C Cuts at: 1132 4414 4874 1132 Size: 3282 460 3774 Fragments arranged by size: 3774 3282 460 SspI AAT'ATT Cuts at: 5 52 5440 5 Size: 47 5388 2081 Fragments arranged by size: 5388 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7516 StyI C'CwwG_G Cuts at: 513 1065 2004 4955 513 Size: 552 939 2951 3074 Fragments arranged by size: 3074 2951 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2095 3147 3704 4091 4312 5543 5560 Size: 1255 515 1052 557 387 221 1231 17 Cuts at: 5560 5713 5898 6343 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1231 1052 557 515 445 387 221 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3005 3882 4155 Size: 274 80 33 51 1443 1028 877 273 Cuts at: 4155 5762 7474 96 Size: 1607 1712 138 Fragments arranged by size: 1712 1607 1443 1028 877 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 2940 2972 7476 7508 2769 Size: 32 139 32 4504 32 2777 Fragments arranged by size: 4504 2777 139 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7516 XbaI T'CTAG_A Cuts at: 4852 4852 Size: 7516 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2884 2884 Size: 7516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7516 XmaI C'CCGG_G Cuts at: 1870 4841 1870 Size: 2971 4545 XmnI GAAnn'nnTTC Cuts at: 2423 5645 2423 Size: 3222 4294 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5324 276 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI