Restriction map for
pFN21AE0061
(Linear) (Six-base) MAPSORT of: KIEE0061.seq Check: 2873 from: 1 to: 2811
KIEE0061.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1644 2811
Size: 1644 1167
AccI GT'mk_AC
Cuts at: 0 739 1378 2811
Size: 739 639 1433
Fragments arranged by size:
1433 739 639
Acc65I G'GTAC_C
Cuts at: 0 1096 2811
Size: 1096 1715
AclI AA'CG_TT
Cuts at: 0 761 2811
Size: 761 2050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2298 2811
Size: 2298 513
AfeI AGC'GCT
Cuts at: 0 1233 2811
Size: 1233 1578
AflII C'TTAA_G
Cuts at: 0 891 2811
Size: 891 1920
AflIII A'CryG_T
Cuts at: 0 1520 1811 2090 2811
Size: 1520 291 279 721
Fragments arranged by size:
1520 721 291 279
AgeI A'CCGG_T
Cuts at: 0 1836 2811
Size: 1836 975
AleI CACnn'nnGTG
Cuts at: 0 2215 2811
Size: 2215 596
AlwNI CAG_nnn'CTG
Cuts at: 0 153 2811
Size: 153 2658
ApaI G_GGCC'C
Cuts at: 0 2290 2811
Size: 2290 521
ApaLI G'TGCA_C
Cuts at: 0 1528 2419 2811
Size: 1528 891 392
Fragments arranged by size:
1528 891 392
ApoI r'AATT_y
Cuts at: 0 205 258 2620 2811
Size: 205 53 2362 191
Fragments arranged by size:
2362 205 191 53
AvaI C'yCGr_G
Cuts at: 0 106 658 1372 1703 2811
Size: 106 552 714 331 1108
Fragments arranged by size:
1108 714 552 331 106
BanI G'GyrC_C
Cuts at: 0 177 670 1096 1698 2022 2811
Size: 177 493 426 602 324 789
Fragments arranged by size:
789 602 493 426 324 177
BanII G_rGCy'C
Cuts at: 0 520 1294 1908 1970 2290 2811
Size: 520 774 614 62 320 521
Fragments arranged by size:
774 614 521 520 320 62
BbeI G_GCGC'C
Cuts at: 0 1702 2811
Size: 1702 1109
BciVI GTATCCnnnnn_n'
Cuts at: 0 10 2496 2811
Size: 10 2486 315
Fragments arranged by size:
2486 315 10
BclI T'GATC_A
Cuts at: 0 2099 2466 2811
Size: 2099 367 345
Fragments arranged by size:
2099 367 345
BlpI GC'TnA_GC
Cuts at: 0 2169 2811
Size: 2169 642
Bme1580I G_kGCm'C
Cuts at: 0 10 1532 2290 2423 2811
Size: 10 1522 758 133 388
Fragments arranged by size:
1522 758 388 133 10
BmgBI CAC'GTC
Cuts at: 0 360 543 1341 2811
Size: 360 183 798 1470
Fragments arranged by size:
1470 798 360 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 712 949 2811
Size: 712 237 1862
Fragments arranged by size:
1862 712 237
BmtI G_CTAG'C
Cuts at: 0 1319 2811
Size: 1319 1492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 106 2811
Size: 106 2705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 828 842 1603 2208 2513
Size: 181 63 93 491 14 761 605 305
Cuts at: 2513 2811
Size: 298
Fragments arranged by size:
761 605 491 305 298 181 93 63
14
BsaI GGTCTCn'nnnn_
Cuts at: 0 2062 2716 2811
Size: 2062 654 95
Fragments arranged by size:
2062 654 95
BsaAI yAC'GTr
Cuts at: 0 1521 1783 2811
Size: 1521 262 1028
Fragments arranged by size:
1521 1028 262
BsaHI Gr'CG_yC
Cuts at: 0 1085 1168 1699 2032 2811
Size: 1085 83 531 333 779
Fragments arranged by size:
1085 779 531 333 83
BsaWI w'CCGG_w
Cuts at: 0 390 615 1836 2596 2811
Size: 390 225 1221 760 215
Fragments arranged by size:
1221 760 390 225 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 826 856 2374 2404 2811
Size: 826 30 1518 30 407
Fragments arranged by size:
1518 826 407 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 292 1591 2443 2734 2811
Size: 124 168 1299 852 291 77
Fragments arranged by size:
1299 852 291 168 124 77
BseYI C'CCAG_C
Cuts at: 0 399 2252 2320 2811
Size: 399 1853 68 491
Fragments arranged by size:
1853 491 399 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1805 2811
Size: 324 1481 1006
Fragments arranged by size:
1481 1006 324
BsiEI CG_ry'CG
Cuts at: 0 273 1847 2811
Size: 273 1574 964
Fragments arranged by size:
1574 964 273
BsiHKAI G_wGCw'C
Cuts at: 0 945 1532 2170 2224 2278 2423 2811
Size: 945 587 638 54 54 145 388
Fragments arranged by size:
945 638 587 388 145 54 54
BsmI GAATG_Cn'
Cuts at: 0 2438 2811
Size: 2438 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1909 2811
Size: 1909 902
Bsp1286I G_dGCh'C
Cuts at: 0 10 520 945 1294 1532 1908 1970 2170
Size: 10 510 425 349 238 376 62 200
Cuts at: 2170 2224 2278 2290 2423 2811
Size: 54 54 12 133 388
Fragments arranged by size:
510 425 388 376 349 238 200 133
62 54 54 12 10
BspEI T'CCGG_A
Cuts at: 0 615 2811
Size: 615 2196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 552 1165 1644 2811
Size: 333 219 613 479 1167
Fragments arranged by size:
1167 613 479 333 219
BsrBI CCG'CTC
Cuts at: 0 1541 2811
Size: 1541 1270
BsrDI GCAATG_nn'
Cuts at: 0 2054 2811
Size: 2054 757
BsrFI r'CCGG_y
Cuts at: 0 1686 1836 1846 2811
Size: 1686 150 10 965
Fragments arranged by size:
1686 965 150 10
BsrGI T'GTAC_A
Cuts at: 0 1000 1877 2150 2811
Size: 1000 877 273 661
Fragments arranged by size:
1000 877 661 273
BssHII G'CGCG_C
Cuts at: 0 1503 1505 1607 1609 2811
Size: 1503 2 102 2 1202
Fragments arranged by size:
1503 1202 102 2 2
BssSI C'ACGA_G
Cuts at: 0 1573 1901 2811
Size: 1573 328 910
Fragments arranged by size:
1573 910 328
BstEII G'GTnAC_C
Cuts at: 0 1152 1272 2811
Size: 1152 120 1539
Fragments arranged by size:
1539 1152 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1995 2130 2232 2811
Size: 1995 135 102 579
Fragments arranged by size:
1995 579 135 102
BstYI r'GATC_y
Cuts at: 0 216 950 2635 2811
Size: 216 734 1685 176
Fragments arranged by size:
1685 734 216 176
BstZ17I GTA'TAC
Cuts at: 0 740 1379 2811
Size: 740 639 1432
Fragments arranged by size:
1432 740 639
Bsu36I CC'TnA_GG
Cuts at: 0 2330 2811
Size: 2330 481
BtgI C'CryG_G
Cuts at: 0 210 1325 1744 1959 2811
Size: 210 1115 419 215 852
Fragments arranged by size:
1115 852 419 215 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 408 1628 2088 2811
Size: 126 282 1220 460 723
Fragments arranged by size:
1220 723 460 282 126
BtsI GCAGTG_nn'
Cuts at: 0 1310 1690 1733 2479 2811
Size: 1310 380 43 746 332
Fragments arranged by size:
1310 746 380 332 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 192 227 2811
Size: 192 35 2584
Fragments arranged by size:
2584 192 35
DraI TTT'AAA
Cuts at: 0 2568 2811
Size: 2568 243
EaeI y'GGCC_r
Cuts at: 0 638 1334 1844 2016 2476 2811
Size: 638 696 510 172 460 335
Fragments arranged by size:
696 638 510 460 335 172
EagI C'GGCC_G
Cuts at: 0 1844 2811
Size: 1844 967
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 184 306 1245 2811
Size: 184 122 939 1566
Fragments arranged by size:
1566 939 184 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 244 337 828 842 1603 2208 2298
Size: 181 63 93 491 14 761 605 90
Cuts at: 2298 2513 2811
Size: 215 298
Fragments arranged by size:
761 605 491 298 215 181 93 90
63 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2329 2428 2811
Size: 2329 99 383
Fragments arranged by size:
2329 383 99
EcoO109I rG'GnC_Cy
Cuts at: 0 150 2286 2461 2811
Size: 150 2136 175 350
Fragments arranged by size:
2136 350 175 150
EcoRI G'AATT_C
Cuts at: 0 258 2811
Size: 258 2553
EcoRV GAT'ATC
Cuts at: 0 483 2811
Size: 483 2328
HaeII r_GCGC'y
Cuts at: 0 1235 1702 2176 2811
Size: 1235 467 474 635
Fragments arranged by size:
1235 635 474 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 853 885 1237 1269 1276 1308
Size: 454 32 367 32 352 32 7 32
Cuts at: 1308 1783 1815 2811
Size: 475 32 996
Fragments arranged by size:
996 475 454 367 352 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 1049 1143 1791 2088 2547 2811
Size: 1049 94 648 297 459 264
Fragments arranged by size:
1049 648 459 297 264 94
KasI G'GCGC_C
Cuts at: 0 1698 2811
Size: 1698 1113
KpnI G_GTAC'C
Cuts at: 0 1100 2811
Size: 1100 1711
MluI A'CGCG_T
Cuts at: 0 1811 2811
Size: 1811 1000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1547 2811
Size: 1547 1264
MscI TGG'CCA
Cuts at: 0 2478 2811
Size: 2478 333
MslI CAynn'nnrTG
Cuts at: 0 791 2215 2811
Size: 791 1424 596
Fragments arranged by size:
1424 791 596
MspA1I CmG'CkG
Cuts at: 0 339 1746 2558 2811
Size: 339 1407 812 253
Fragments arranged by size:
1407 812 339 253
NaeI GCC'GGC
Cuts at: 0 1688 2811
Size: 1688 1123
NarI GG'CG_CC
Cuts at: 0 1699 2811
Size: 1699 1112
NdeI CA'TA_TG
Cuts at: 0 920 2811
Size: 920 1891
NgoMIV G'CCGG_C
Cuts at: 0 1686 2811
Size: 1686 1125
NheI G'CTAG_C
Cuts at: 0 1315 2811
Size: 1315 1496
NspI r_CATG'y
Cuts at: 0 2195 2409 2811
Size: 2195 214 402
Fragments arranged by size:
2195 402 214
PasI CC'CwG_GG
Cuts at: 0 25 2811
Size: 25 2786
PflMI CCAn_nnn'nTGG
Cuts at: 0 1770 2811
Size: 1770 1041
PfoI T'CCnGG_A
Cuts at: 0 536 2454 2811
Size: 536 1918 357
Fragments arranged by size:
1918 536 357
PpuMI rG'GwC_Cy
Cuts at: 0 150 2811
Size: 150 2661
PsiI TTA'TAA
Cuts at: 0 55 2811
Size: 55 2756
PspOMI G'GGCC_C
Cuts at: 0 2286 2811
Size: 2286 525
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 723 755 1369 1401 2811
Size: 723 32 614 32 1410
Fragments arranged by size:
1410 723 614 32 32
PstI C_TGCA'G
Cuts at: 0 239 344 1562 1655 2811
Size: 239 105 1218 93 1156
Fragments arranged by size:
1218 1156 239 105 93
PvuII CAG'CTG
Cuts at: 0 2558 2811
Size: 2558 253
SacII CC_GC'GG
Cuts at: 0 1747 2811
Size: 1747 1064
SexAI A'CCwGG_T
Cuts at: 0 1092 2811
Size: 1092 1719
SfcI C'TryA_G
Cuts at: 0 235 340 1558 1651 2345 2811
Size: 235 105 1218 93 694 466
Fragments arranged by size:
1218 694 466 235 105 93
SfoI GGC'GCC
Cuts at: 0 1700 2811
Size: 1700 1111
SmlI C'TyrA_G
Cuts at: 0 891 2811
Size: 891 1920
SphI G_CATG'C
Cuts at: 0 2409 2811
Size: 2409 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 90 1142 1699 2086 2307 2811
Size: 90 1052 557 387 221 504
Fragments arranged by size:
1052 557 504 387 221 90
TatI w'GTAC_w
Cuts at: 0 1000 1877 2150 2811
Size: 1000 877 273 661
Fragments arranged by size:
1000 877 661 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 935 967 2811
Size: 764 32 139 32 1844
Fragments arranged by size:
1844 764 139 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 2811
Size: 879 1932
XmnI GAAnn'nnTTC
Cuts at: 0 418 2811
Size: 418 2393
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BciVI BclI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI EaeI EagI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NspI PasI PflMI PfoI PpuMI PsiI PspOMI
PsrI PstI PvuII SacII SexAI SfcI SfoI SmlI
SphI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BfrBI BglI BglII
Bpu10I BpuEI BsaBI BsiWI BspHI BstAPI BstBI ClaI
DraIII DrdI EarI EcoICRI FalI FseI FspI FspAI
HindIII HpaI MfeI NcoI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PspXI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmaI ZraI