(Circular) (Six-base) MAPSORT of: pFN21AE0060.seq Check: 8390 from: 1 to: 6430 pFN21AE0060 6430 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3673 3673 Size: 6430 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3222 3579 4493 5877 6145 1632 Size: 1590 357 914 1384 268 1917 Fragments arranged by size: 1917 1590 1384 914 357 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 2671 2694 5329 6306 1203 Size: 1468 23 2635 977 1327 Fragments arranged by size: 2635 1468 1327 977 23 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2088 3052 4173 5745 2088 Size: 964 1121 1572 2773 Fragments arranged by size: 2773 1572 1121 964 ApaI G_GGCC'C Cuts at: 2158 2158 Size: 6430 ApaLI G'TGCA_C Cuts at: 4487 5643 4487 Size: 1156 5274 ApoI r'AATT_y Cuts at: 1493 2013 2567 3739 3965 4117 6226 1493 Size: 520 554 1172 226 152 2109 1697 Fragments arranged by size: 2109 1697 1172 554 520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2782 3755 1870 Size: 73 208 631 973 4545 Fragments arranged by size: 4545 973 631 208 73 AvrII C'CTAG_G Cuts at: 3068 3068 Size: 6430 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3751 5206 618 Size: 325 217 627 1964 1455 1842 Fragments arranged by size: 1964 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2158 2992 3749 1391 Size: 767 834 757 4072 Fragments arranged by size: 4072 834 767 757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 2743 3552 928 Size: 971 416 428 809 3806 Fragments arranged by size: 3806 971 809 428 416 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4322 5532 3377 Size: 945 1210 4275 Fragments arranged by size: 4275 1210 945 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5041 136 Size: 107 122 71 631 605 3369 1525 Fragments arranged by size: 3369 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3127 6425 3127 Size: 3298 3132 BlpI GC'TnA_GC Cuts at: 3847 3847 Size: 6430 Bme1580I G_kGCm'C Cuts at: 1790 2158 4491 5647 1790 Size: 368 2333 1156 2573 Fragments arranged by size: 2573 2333 1156 368 BmgBI CAC'GTC Cuts at: 1318 2674 1318 Size: 1356 5074 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2534 4161 5119 Size: 416 53 83 399 24 1117 1627 958 Cuts at: 5119 442 Size: 1753 Fragments arranged by size: 1753 1627 1117 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3099 3412 3658 5090 1239 Size: 1860 313 246 1432 2579 Fragments arranged by size: 2579 1860 1432 313 246 Bpu10I CC'TnA_GC Cuts at: 3099 3099 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2206 2561 4555 5420 5718 2206 Size: 355 1994 865 298 2918 Fragments arranged by size: 2918 1994 865 355 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2852 5093 882 Size: 617 1353 2241 2219 Fragments arranged by size: 2241 2219 1353 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 3281 6424 3281 Size: 3143 3287 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4237 4619 5254 275 Size: 53 83 186 936 2704 382 635 1451 Fragments arranged by size: 2704 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 2443 3707 4863 5261 5535 5682 6093 2094 Size: 349 1264 1156 398 274 147 411 2431 Fragments arranged by size: 2431 1264 1156 411 398 349 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2799 2829 3095 3125 3410 Size: 30 165 30 1396 30 266 30 285 Cuts at: 3410 3440 3505 3535 1178 Size: 30 65 30 4073 Fragments arranged by size: 4073 1396 285 266 165 65 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2820 2910 1186 Size: 1147 487 90 4706 Fragments arranged by size: 4706 1147 487 90 BseYI C'CCAG_C Cuts at: 2059 3230 3259 5633 6129 2059 Size: 1171 29 2374 496 2360 Fragments arranged by size: 2374 2360 1171 496 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5857 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4491 4576 5647 4491 Size: 85 1071 5274 Fragments arranged by size: 5274 1071 85 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 BsmBI CGTCTCn'nnnn_ Cuts at: 2620 2620 Size: 6430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2992 3749 4491 4576 5647 1391 Size: 399 368 834 757 742 85 1071 2174 Fragments arranged by size: 2174 1071 834 757 742 399 368 85 BspEI T'CCGG_A Cuts at: 2094 2443 6093 2094 Size: 349 3650 2431 Fragments arranged by size: 3650 2431 349 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3673 3785 844 Size: 2829 112 3489 Fragments arranged by size: 3489 2829 112 BsrBI CCG'CTC Cuts at: 1394 1823 2136 2257 4317 1394 Size: 429 313 121 2060 3507 Fragments arranged by size: 3507 2060 429 313 121 BsrDI GCAATG_nn' Cuts at: 65 1248 3195 3460 3688 4925 5099 65 Size: 1183 1947 265 228 1237 174 1396 Fragments arranged by size: 1947 1396 1237 1183 265 228 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2242 3515 5074 5261 1700 Size: 110 432 1273 1559 187 2869 Fragments arranged by size: 2869 1559 1273 432 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 3197 794 Size: 2403 4027 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 2401 1184 Size: 1217 5213 BstYI r'GATC_y Cuts at: 1972 3082 3127 3760 4127 4512 4529 5981 6067 Size: 1110 45 633 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1110 633 385 367 346 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2811 3377 3462 513 Size: 552 1746 566 85 3481 Fragments arranged by size: 3481 1746 566 552 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2401 487 Size: 683 56 1175 4516 Fragments arranged by size: 4516 1175 683 56 BtsI GCAGTG_nn' Cuts at: 3946 4740 4760 3946 Size: 794 20 5616 Fragments arranged by size: 5616 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 2565 3734 2565 Size: 1169 5261 DraIII CAC_nnn'GTG Cuts at: 2476 2476 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2120 2381 5437 809 Size: 507 804 261 3056 1802 Fragments arranged by size: 3056 1802 804 507 261 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3465 3877 4766 Size: 54 1425 50 275 18 1635 412 889 Cuts at: 4766 6395 8 Size: 1629 43 Fragments arranged by size: 1635 1629 1425 889 412 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2026 3394 3598 3614 4360 1353 Size: 673 1368 204 16 746 3423 Fragments arranged by size: 3423 1368 746 673 204 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3099 3222 3412 3579 3658 4493 5090 Size: 393 1467 123 190 167 79 835 597 Cuts at: 5090 5877 6145 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1467 835 787 597 393 268 190 167 123 79 EcoO109I rG'GnC_Cy Cuts at: 2085 2374 3645 2085 Size: 289 1271 4870 Fragments arranged by size: 4870 1271 289 EcoRI G'AATT_C Cuts at: 2013 3739 4117 2013 Size: 1726 378 4326 Fragments arranged by size: 4326 1726 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3643 3643 Size: 6430 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2222 5577 1678 Size: 544 3355 2531 Fragments arranged by size: 3355 2531 544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 2516 2548 2799 Size: 32 135 32 898 32 14 32 251 Cuts at: 2799 2831 5071 5103 5145 5177 1373 Size: 32 2240 32 42 32 2626 Fragments arranged by size: 2626 2240 898 251 135 42 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2649 3327 3774 4020 669 Size: 988 281 711 678 447 246 3079 Fragments arranged by size: 3079 988 711 678 447 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2083 2205 2220 5544 5728 1305 Size: 778 122 15 3324 184 2007 Fragments arranged by size: 3324 2007 778 184 122 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 3467 10 Size: 54 1425 343 1635 2973 Fragments arranged by size: 2973 1635 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4388 4747 4906 518 Size: 648 3222 359 159 2042 Fragments arranged by size: 3222 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3379 3678 3846 4523 5671 5916 1606 Size: 1773 299 168 677 1148 245 2120 Fragments arranged by size: 2120 1773 1148 677 299 245 168 NcoI C'CATG_G Cuts at: 513 1065 2811 3462 513 Size: 552 1746 651 3481 Fragments arranged by size: 3481 1746 651 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2698 3788 5333 1132 Size: 97 1469 1090 1545 2229 Fragments arranged by size: 2229 1545 1469 1090 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6430 PciI A'CATG_T Cuts at: 2694 5329 2694 Size: 2635 3795 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 5293 PfoI T'CCnGG_A Cuts at: 3076 3076 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2361 2393 4499 4531 6012 6044 2361 Size: 32 2106 32 1481 32 2747 Fragments arranged by size: 2747 2106 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2085 2374 2085 Size: 289 6141 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 2964 4000 2964 Size: 1036 5394 PspOMI G'GGCC_C Cuts at: 2154 2154 Size: 6430 PstI C_TGCA'G Cuts at: 830 3057 3127 3782 830 Size: 2227 70 655 3478 Fragments arranged by size: 3478 2227 655 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 SacII CC_GC'GG Cuts at: 3380 3380 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 2347 3053 3123 3211 3448 3778 4913 Size: 219 1302 706 70 88 237 330 1135 Cuts at: 4913 5594 5785 826 Size: 681 191 1471 Fragments arranged by size: 1471 1302 1135 706 681 330 237 219 191 88 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 2153 3757 1872 Size: 281 1604 4545 Fragments arranged by size: 4545 1604 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 2185 2576 4534 5435 5697 820 Size: 197 926 242 391 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 391 262 242 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6430 StyI C'CwwG_G Cuts at: 513 1065 2615 2811 3068 3462 3649 3869 513 Size: 552 1550 196 257 394 187 220 3074 Fragments arranged by size: 3074 1550 552 394 257 220 196 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2068 2389 3464 3526 4457 4474 4627 Size: 1255 488 321 1075 62 931 17 153 Cuts at: 4627 4812 5257 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1075 931 488 445 321 185 153 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2911 4676 6388 Size: 274 80 33 51 1443 934 1765 1712 Cuts at: 6388 96 Size: 138 Fragments arranged by size: 1765 1712 1443 934 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3505 3537 6390 6422 3505 Size: 32 2853 32 3513 Fragments arranged by size: 3513 2853 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 2540 1316 Size: 1083 141 5206 Fragments arranged by size: 5206 1083 141 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2858 3469 2858 Size: 611 5819 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 2151 3755 1870 Size: 281 1604 4545 Fragments arranged by size: 4545 1604 281 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI