Restriction map for
pFN21AE0060
(Linear) (Six-base) MAPSORT of: KIEE0060.seq Check: 5298 from: 1 to: 1725
KIEE0060.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1668 1725
Size: 1668 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 1574 1725
Size: 1217 357 151
Fragments arranged by size:
1217 357 151
AflIII A'CryG_T
Cuts at: 0 666 689 1725
Size: 666 23 1036
Fragments arranged by size:
1036 666 23
AlwNI CAG_nnn'CTG
Cuts at: 0 83 1047 1725
Size: 83 964 678
Fragments arranged by size:
964 678 83
ApaI G_GGCC'C
Cuts at: 0 153 1725
Size: 153 1572
ApoI r'AATT_y
Cuts at: 0 8 562 1725
Size: 8 554 1163
Fragments arranged by size:
1163 554 8
AvaI C'yCGr_G
Cuts at: 0 146 777 1725
Size: 146 631 948
Fragments arranged by size:
948 631 146
AvrII C'CTAG_G
Cuts at: 0 1063 1725
Size: 1063 662
BanII G_rGCy'C
Cuts at: 0 153 987 1725
Size: 153 834 738
Fragments arranged by size:
834 738 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 738 1547 1725
Size: 310 428 809 178
Fragments arranged by size:
809 428 310 178
BciVI GTATCCnnnnn_n'
Cuts at: 0 1372 1725
Size: 1372 353
BglII A'GATC_T
Cuts at: 0 1122 1725
Size: 1122 603
Bme1580I G_kGCm'C
Cuts at: 0 153 1725
Size: 153 1572
BmgBI CAC'GTC
Cuts at: 0 669 1725
Size: 669 1056
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 1725
Size: 529 1196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1407 1653 1725
Size: 1094 313 246 72
Fragments arranged by size:
1094 313 246 72
Bpu10I CC'TnA_GC
Cuts at: 0 1094 1725
Size: 1094 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 556 1725
Size: 201 355 1169
Fragments arranged by size:
1169 355 201
BsaI GGTCTCn'nnnn_
Cuts at: 0 847 1725
Size: 847 878
BsaBI GATnn'nnATC
Cuts at: 0 1276 1725
Size: 1276 449
BsaWI w'CCGG_w
Cuts at: 0 89 438 1702 1725
Size: 89 349 1264 23
Fragments arranged by size:
1264 349 89 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 794 824 1090 1120 1405 1435 1500 1530
Size: 794 30 266 30 285 30 65 30
Cuts at: 1530 1725
Size: 195
Fragments arranged by size:
794 285 266 195 65 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 815 905 1725
Size: 328 487 90 820
Fragments arranged by size:
820 487 328 90
BseYI C'CCAG_C
Cuts at: 0 54 1225 1254 1725
Size: 54 1171 29 471
Fragments arranged by size:
1171 471 54 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 615 1725
Size: 615 1110
Bsp1286I G_dGCh'C
Cuts at: 0 153 987 1725
Size: 153 834 738
Fragments arranged by size:
834 738 153
BspEI T'CCGG_A
Cuts at: 0 89 438 1725
Size: 89 349 1287
Fragments arranged by size:
1287 349 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1668 1725
Size: 1668 57
BsrBI CCG'CTC
Cuts at: 0 131 252 1725
Size: 131 121 1473
Fragments arranged by size:
1473 131 121
BsrDI GCAATG_nn'
Cuts at: 0 1190 1455 1683 1725
Size: 1190 265 228 42
Fragments arranged by size:
1190 265 228 42
BsrFI r'CCGG_y
Cuts at: 0 237 1510 1725
Size: 237 1273 215
Fragments arranged by size:
1273 237 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1192 1725
Size: 1192 533
BstEII G'GTnAC_C
Cuts at: 0 396 1725
Size: 396 1329
BstYI r'GATC_y
Cuts at: 0 1077 1122 1725
Size: 1077 45 603
Fragments arranged by size:
1077 603 45
BtgI C'CryG_G
Cuts at: 0 806 1372 1457 1725
Size: 806 566 85 268
Fragments arranged by size:
806 566 268 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 396 1725
Size: 396 1329
DraI TTT'AAA
Cuts at: 0 560 1725
Size: 560 1165
DraIII CAC_nnn'GTG
Cuts at: 0 471 1725
Size: 471 1254
DrdI GACnn_nn'nnGTC
Cuts at: 0 115 376 1725
Size: 115 261 1349
Fragments arranged by size:
1349 261 115
EaeI y'GGCC_r
Cuts at: 0 1460 1725
Size: 1460 265
EarI CTCTTCn'nnn_
Cuts at: 0 21 1389 1593 1609 1725
Size: 21 1368 204 16 116
Fragments arranged by size:
1368 204 116 21 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1094 1217 1407 1574 1653 1725
Size: 1094 123 190 167 79 72
Fragments arranged by size:
1094 190 167 123 79 72
EcoO109I rG'GnC_Cy
Cuts at: 0 80 369 1640 1725
Size: 80 289 1271 85
Fragments arranged by size:
1271 289 85 80
EcoRI G'AATT_C
Cuts at: 0 8 1725
Size: 8 1717
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1638 1725
Size: 1638 87
HaeII r_GCGC'y
Cuts at: 0 217 1725
Size: 217 1508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 511 543 794 826 1725
Size: 465 32 14 32 251 32 899
Fragments arranged by size:
899 465 251 32 32 32 14
HincII GTy'rAC
Cuts at: 0 644 1322 1725
Size: 644 678 403
Fragments arranged by size:
678 644 403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 200 215 1725
Size: 78 122 15 1510
Fragments arranged by size:
1510 122 78 15
MscI TGG'CCA
Cuts at: 0 1462 1725
Size: 1462 263
MspA1I CmG'CkG
Cuts at: 0 1374 1673 1725
Size: 1374 299 52
Fragments arranged by size:
1374 299 52
NcoI C'CATG_G
Cuts at: 0 806 1457 1725
Size: 806 651 268
Fragments arranged by size:
806 651 268
NspI r_CATG'y
Cuts at: 0 693 1725
Size: 693 1032
PciI A'CATG_T
Cuts at: 0 689 1725
Size: 689 1036
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1725
Size: 240 1485
PfoI T'CCnGG_A
Cuts at: 0 1071 1725
Size: 1071 654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 1725
Size: 356 32 1337
Fragments arranged by size:
1337 356 32
PpuMI rG'GwC_Cy
Cuts at: 0 80 369 1725
Size: 80 289 1356
Fragments arranged by size:
1356 289 80
PsiI TTA'TAA
Cuts at: 0 959 1725
Size: 959 766
PspOMI G'GGCC_C
Cuts at: 0 149 1725
Size: 149 1576
PstI C_TGCA'G
Cuts at: 0 1052 1122 1725
Size: 1052 70 603
Fragments arranged by size:
1052 603 70
SacII CC_GC'GG
Cuts at: 0 1375 1725
Size: 1375 350
SfcI C'TryA_G
Cuts at: 0 342 1048 1118 1206 1443 1725
Size: 342 706 70 88 237 282
Fragments arranged by size:
706 342 282 237 88 70
SmaI CCC'GGG
Cuts at: 0 148 1725
Size: 148 1577
SmlI C'TyrA_G
Cuts at: 0 180 571 1725
Size: 180 391 1154
Fragments arranged by size:
1154 391 180
StyI C'CwwG_G
Cuts at: 0 610 806 1063 1457 1644 1725
Size: 610 196 257 394 187 81
Fragments arranged by size:
610 394 257 196 187 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 384 1459 1521 1725
Size: 63 321 1075 62 204
Fragments arranged by size:
1075 321 204 63 62
TatI w'GTAC_w
Cuts at: 0 906 1725
Size: 906 819
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1500 1532 1725
Size: 1500 32 193
Fragments arranged by size:
1500 193 32
Tth111I GACn'n_nGTC
Cuts at: 0 394 535 1725
Size: 394 141 1190
Fragments arranged by size:
1190 394 141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 853 1464 1725
Size: 853 611 261
Fragments arranged by size:
853 611 261
XmaI C'CCGG_G
Cuts at: 0 146 1725
Size: 146 1579
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApoI AvaI AvrII
BanII BbsI BciVI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstYI BtgI BtgZI DraI DraIII DrdI EaeI
EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
MmeI MscI MspA1I NcoI NspI PciI PflMI PfoI
PpiI PpuMI PsiI PspOMI PstI SacII SfcI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI
BlpI BmtI BplI BsaAI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmI BspHI BsrGI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtsI ClaI CspCI EagI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmnI ZraI