(Circular) (Six-base) MAPSORT of: pFN21AE0059.seq Check: 9025 from: 1 to: 6130 pFN21AE0059 6130 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2692 4193 5577 5845 1632 Size: 1060 1501 1384 268 1917 Fragments arranged by size: 1917 1501 1384 1060 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2638 5029 6006 1203 Size: 1435 2391 977 1327 Fragments arranged by size: 2391 1435 1327 977 AgeI A'CCGG_T Cuts at: 2750 4961 2750 Size: 2211 3919 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 2835 3087 3171 3339 1166 Size: 1669 252 84 168 3957 Fragments arranged by size: 3957 1669 252 168 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 3873 5445 3873 Size: 1572 4558 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2574 2618 2658 2826 2910 2994 3078 3162 Size: 1081 44 40 168 84 84 84 84 Cuts at: 3162 3246 3330 3414 3439 3665 3817 5926 1493 Size: 84 84 84 25 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 226 168 152 84 84 84 84 84 84 84 44 40 25 AseI AT'TA_AT Cuts at: 160 1032 2493 4684 160 Size: 872 1461 2191 1606 Fragments arranged by size: 2191 1606 1461 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2196 2888 2972 3140 3449 1391 Size: 805 692 84 168 309 4072 Fragments arranged by size: 4072 805 692 309 168 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2508 928 Size: 971 609 4550 Fragments arranged by size: 4550 971 609 BbvCI CC'TCA_GC Cuts at: 2010 2010 Size: 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2236 6125 2236 Size: 3889 2241 BlpI GC'TnA_GC Cuts at: 3055 3547 3055 Size: 492 5638 Bme1580I G_kGCm'C Cuts at: 1790 4191 5347 1790 Size: 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2405 3861 4819 Size: 416 53 83 399 24 988 1456 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1456 988 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2332 4790 1239 Size: 1093 2458 2579 Fragments arranged by size: 2579 2458 1093 Bpu10I CC'TnA_GC Cuts at: 2010 2154 2010 Size: 144 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2145 2360 4255 5120 5418 2145 Size: 215 1895 865 298 2857 Fragments arranged by size: 2857 1895 865 298 215 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2750 4563 4961 5235 5382 5793 2750 Size: 1813 398 274 147 411 3087 Fragments arranged by size: 3087 1813 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5244 BseYI C'CCAG_C Cuts at: 5333 5829 5333 Size: 496 5634 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2888 2972 3140 4191 4276 5347 2888 Size: 84 168 1051 85 1071 3671 Fragments arranged by size: 3671 1071 1051 168 85 84 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2888 2972 3140 3449 4191 4276 Size: 399 406 692 84 168 309 742 85 Cuts at: 4276 5347 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 692 406 399 309 168 85 84 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2750 4774 4961 1700 Size: 110 940 2024 187 2869 Fragments arranged by size: 2869 2024 940 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2236 3394 3460 3827 4212 4229 5681 5767 Size: 264 1158 66 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 264 86 66 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4440 4460 3646 Size: 794 20 5316 Fragments arranged by size: 5316 794 20 ClaI AT'CG_AT Cuts at: 1554 2246 1554 Size: 692 5438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3013 3048 529 Size: 35 2449 35 3611 Fragments arranged by size: 3611 2449 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4060 1353 Size: 2707 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2886 2970 3138 2886 Size: 84 168 5878 Fragments arranged by size: 5878 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2332 2692 4193 4790 5577 5845 1239 Size: 393 700 360 1501 597 787 268 1524 Fragments arranged by size: 1524 1501 787 700 597 393 360 268 EcoRI G'AATT_C Cuts at: 2574 2826 2910 2994 3078 3162 3246 3330 3414 Size: 252 84 84 84 84 84 84 84 Cuts at: 3414 3439 3817 2574 Size: 25 378 4887 Fragments arranged by size: 4887 378 252 84 84 84 84 84 84 84 25 EcoRV GAT'ATC Cuts at: 2402 2402 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 4771 4803 4845 Size: 32 135 32 656 32 2511 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 2511 656 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3152 3474 3720 669 Size: 988 281 1214 322 246 3079 Fragments arranged by size: 3079 1214 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 2975 748 Size: 2227 3903 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5244 5428 1305 Size: 786 3153 184 2007 Fragments arranged by size: 3153 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 2835 2919 3003 3087 3171 3255 Size: 648 1417 252 84 84 84 84 84 Cuts at: 3255 3271 3339 4088 4447 4606 518 Size: 16 68 749 359 159 2042 Fragments arranged by size: 2042 1417 749 648 359 252 159 84 84 84 84 84 68 16 MspA1I CmG'CkG Cuts at: 1606 3546 4223 5371 5616 1606 Size: 1940 677 1148 245 2120 Fragments arranged by size: 2120 1940 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 3268 387 Size: 2881 3249 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2449 2642 2906 3488 5033 1132 Size: 97 1220 193 264 582 1545 2229 Fragments arranged by size: 2229 1545 1220 582 264 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2638 5029 2638 Size: 2391 3739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SacI G_AGCT'C Cuts at: 2888 2972 3140 2888 Size: 84 168 5878 Fragments arranged by size: 5878 168 84 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 2345 4378 6090 2345 Size: 2033 1712 2385 Fragments arranged by size: 2385 2033 1712 SfcI C'TryA_G Cuts at: 826 1045 3478 4613 5294 5485 826 Size: 219 2433 1135 681 191 1471 Fragments arranged by size: 2433 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 2339 4234 5135 5397 820 Size: 197 926 217 179 1895 901 262 1553 Fragments arranged by size: 1895 1553 926 901 262 217 197 179 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 2906 3488 1132 Size: 1774 582 3774 Fragments arranged by size: 3774 1774 582 SspI AAT'ATT Cuts at: 5 52 2430 4054 5 Size: 47 2378 1624 2081 Fragments arranged by size: 2378 2081 1624 47 StuI AGG'CCT Cuts at: 1517 2226 2537 2705 2789 3209 3293 1517 Size: 709 311 168 84 420 84 4354 Fragments arranged by size: 4354 709 420 311 168 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3569 513 Size: 552 939 1565 3074 Fragments arranged by size: 3074 1565 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4157 4174 4327 4512 4957 325 Size: 1255 2577 17 153 185 445 1498 Fragments arranged by size: 2577 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 4376 6088 Size: 274 80 33 51 1443 366 2033 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 2033 1712 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 3216 3248 6090 6122 2234 Size: 32 950 32 2842 32 2242 Fragments arranged by size: 2842 2242 950 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI