Restriction map for
pFN21AE0056
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I CelII
CfoI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfcI
SfiI SfoI SmaI SmlI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 643 GACGCC G[GA]^CG[CT]C
AlwNI 972 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 122 CCTTAGG CC^T[ACGT]AGG
BanI 231 GGTGCC G^G[CT][GA]CC
BcnI 88 CCCGG CC^[GC]GG
BglII 764 AGATCT A^GATCT
BsaAI 598 TACGTG [CT]AC^GT[GA]
BsaHI 643 GACGCC G[GA]^CG[CT]C
BsaI 603 GGTCTC GGTCTC(1/5)
BseRI 349 CTCCTC GAGGAG(10/8)
BsmBI 567 CGTCTC CGTCTC(1/5)
BsmI 43 GAATGC GAATGC(1/-1)
Bsp1407I 865 TGTACA T^GTACA
BsrDI 480 CATTGC GCAATG(2/0)
BsrFI 460 ACCGGC [GA]^CCGG[CT]
BsrGI 865 TGTACA T^GTACA
BsrI 1091 ACTGG ACTGG(1/-1)
BssSI 301 CACGAG CACGAG(-5/-1)
BstBI 920 TTCGAA TT^CGAA
Bsu36I 122 CCTTAGG CC^T[ACGT]AGG
Cfr10I 460 ACCGGC [GA]^CCGG[CT]
DraI 283 TTTAAA TTT^AAA
EarI 1287 CTCTTC CTCTTC(1/4)
Eco81I 122 CCTTAGG CC^T[ACGT]AGG
EcoNI 185 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 643 GACGC GACGC(5/10)
Hin1I 643 GACGCC G[GA]^CG[CT]C
Ksp632I 1287 CTCTTC CTCTTC(1/4)
KspI 13 CCGCGG CCGC^GG
MspA1I 13 CCGCGG C[AC]G^C[GT]G
NciI 88 CCCGG CC^[GC]GG
NcoI 110 CCATGG C^CATGG
NruI 411 TCGCGA TCG^CGA
NspV 920 TTCGAA TT^CGAA
PflMI 726 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 13 CCGCGG CCGC^GG
SexAI 227 ACCAGGT A^CC[AT]GGT
SfuI 920 TTCGAA TT^CGAA
SgrAI 459 CACCGGCG C[GA]^CCGG[CT]G
SspBI 865 TGTACA T^GTACA
SspI 1171 AATATT AAT^ATT
StuI 1258 AGGCCT AGG^CCT
TfiI 629 GAATC G^A[AT]TC
Van91I 726 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 14 CGCG CG^CG
412 CGCG
AcsI 287 AAATTC [GA]^AATT[CT]
1190 AAATTC
AfaI 866 GTAC GT^AC
1209 GTAC
ApoI 287 AAATTC [GA]^AATT[CT]
1190 AAATTC
BalI 164 TGGCCA TGG^CCA
852 TGGCCA
BamHI 387 GGATCC G^GATCC
1130 GGATCC
BpmI 520 CTGGAG CTGGAG(16/14)
1013 CTGGAG
BsaWI 390 TCCGGT [AT]^CCGG[AT]
1061 ACCGGA
BsgI 369 CTGCAC GTGCAG(16/14)
450 GTGCAG
BspMI 158 ACCTGC ACCTGC(4/8)
319 ACCTGC
BstAPI 207 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
225 GCACCAGGTGC
BstUI 14 CGCG CG^CG
412 CGCG
DraIII 226 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
307 CACGCTGTG
EaeI 164 TGGCCA [CT]^GGCC[GA]
852 TGGCCA
MluNI 164 TGGCCA TGG^CCA
852 TGGCCA
MscI 164 TGGCCA TGG^CCA
852 TGGCCA
MslI 325 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
814 CATTCCAATG
MvnI 14 CGCG CG^CG
412 CGCG
PleI 942 GACTC GAGTC(4/5)
476 GAGTC
PpuMI 260 AGGACCC [GA]G^G[AT]CC[CT]
515 GGGACCT
RsaI 866 GTAC GT^AC
1209 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII