Restriction map for
pFN21AE0056
(Circular) (Six-base) MAPSORT of: pFN21AE0056.seq Check: 9713 from: 1 to: 6121
pFN21AE0056 6121 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2172 2333 2172
Size: 161 5960
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3464 1937
Size: 1527 4594
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2836 4184 5568 5836 1632
Size: 1204 1348 1384 268 1917
Fragments arranged by size:
1917 1384 1348 1204 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 2334 1166
Size: 1168 4953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AlwNI CAG_nnn'CTG
Cuts at: 2982 3864 5436 2982
Size: 882 1572 3667
Fragments arranged by size:
3667 1572 882
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2292 3195 3430 3656 3808 5917 1493
Size: 799 903 235 226 152 2109 1697
Fragments arranged by size:
2109 1697 903 799 235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 3446 1870
Size: 73 1503 4545
Fragments arranged by size:
4545 1503 73
BamHI G'GATC_C
Cuts at: 2392 3135 3451 3818 2392
Size: 743 316 367 4695
Fragments arranged by size:
4695 743 367 316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3442 4897 618
Size: 325 217 627 449 1206 1455 1842
Fragments arranged by size:
1842 1455 1206 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 3440 1391
Size: 2049 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2769 6116 2769
Size: 3347 2774
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 2307 4182 5338 1790
Size: 517 1875 1156 2573
Fragments arranged by size:
2573 1875 1156 517
BmgBI CAC'GTC
Cuts at: 1318 2623 2706 1318
Size: 1305 83 4733
Fragments arranged by size:
4733 1305 83
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3852 4810
Size: 416 53 83 399 24 1689 746 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1689 958 746 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 3039 4781 1239
Size: 1307 493 1742 2579
Fragments arranged by size:
2579 1742 1307 493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2614 4784 882
Size: 617 1115 2170 2219
Fragments arranged by size:
2219 2170 1115 617
BsaAI yAC'GTr
Cuts at: 493 1204 2605 3912 493
Size: 711 1401 1307 2702
Fragments arranged by size:
2702 1401 1307 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2649 3928 4310 4945
Size: 53 83 186 936 1116 1279 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1451 1279 1116 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2395 3066 4554 4952 5226 5373 5784 2395
Size: 671 1488 398 274 147 411 2732
Fragments arranged by size:
2732 1488 671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2928 2958 1178
Size: 30 165 30 855 30 640 30 4341
Fragments arranged by size:
4341 855 640 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 1186
Size: 1159 4962
BseYI C'CCAG_C
Cuts at: 5324 5820 5324
Size: 496 5625
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 2476 1163
Size: 573 623 117 4808
Fragments arranged by size:
4808 623 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2027 2313 2324 2430 4182 4267 5338 2027
Size: 286 11 106 1752 85 1071 2810
Fragments arranged by size:
2810 1752 1071 286 106 85 11
BsmI GAATG_Cn'
Cuts at: 2054 3632 3725 2054
Size: 1578 93 4450
Fragments arranged by size:
4450 1578 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2578 2578
Size: 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2307 2313 2324 2430 3440 4182
Size: 399 237 280 6 11 106 1010 742
Cuts at: 4182 4267 5338 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1010 742 399 280 237 106
85 11 6
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2333 3476 844
Size: 1328 161 1143 3489
Fragments arranged by size:
3489 1328 1143 161
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2484 4616 4790 65
Size: 1183 1236 2132 174 1396
Fragments arranged by size:
2132 1396 1236 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2465 4765 4952 1700
Size: 110 655 2300 187 2869
Fragments arranged by size:
2869 2300 655 187 110
BsrGI T'GTAC_A
Cuts at: 96 2870 96
Size: 2774 3347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 2306 4181 5193 2306
Size: 1875 1012 3234
Fragments arranged by size:
3234 1875 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2218 2236 794
Size: 1424 18 4679
Fragments arranged by size:
4679 1424 18
BstBI TT'CG_AA
Cuts at: 2926 4925 2926
Size: 1999 4122
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 2280 2392 2769 3135 3451 3818 4203 4220
Size: 308 112 377 366 316 367 385 17
Cuts at: 4220 5672 5758 5770 6116 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 377 367 366 346 316
308 112 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2128 2128
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2115 3225 513
Size: 552 939 14 97 1110 3409
Fragments arranged by size:
3409 1110 939 552 97 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 2290 3425 2290
Size: 1135 4986
DraIII CAC_nnn'GTG
Cuts at: 2236 2317 2236
Size: 81 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2857 3568
Size: 54 1425 50 275 18 339 688 711
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 711 688 339 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3298 4051 1353
Size: 1945 753 3423
Fragments arranged by size:
3423 1945 753
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2059 4708 5082 5228 387
Size: 1064 138 470 2649 374 146 1280
Fragments arranged by size:
2649 1280 1064 470 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2836 3039 4184 4781 5568 5836
Size: 393 914 290 203 1145 597 787 268
Cuts at: 5836 1239
Size: 1524
Fragments arranged by size:
1524 1145 914 787 597 393 290 268
203
EcoNI CCTnn'n_nnAGG
Cuts at: 2194 2194
Size: 6121
EcoO109I rG'GnC_Cy
Cuts at: 2124 2266 2514 2521 2124
Size: 142 248 7 5724
Fragments arranged by size:
5724 248 142 7
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2258 2290 2768 2800 3344
Size: 32 135 32 686 32 478 32 544
Cuts at: 3344 3376 4762 4794 4836 4868 1373
Size: 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 686 544 478 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2859 10
Size: 54 1425 343 339 688 3272
Fragments arranged by size:
3272 1425 688 343 339 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 2823 4079 4438 4597 518
Size: 648 1168 489 1256 359 159 2042
Fragments arranged by size:
2042 1256 1168 648 489 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 3537 4214 5362 5607 1606
Size: 414 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2115 513
Size: 552 939 111 4519
Fragments arranged by size:
4519 939 552 111
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NruI TCG'CGA
Cuts at: 2418 2418
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 1108
Size: 1629 4492
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2521 2266
Size: 255 5866
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PstI C_TGCA'G
Cuts at: 830 3473 830
Size: 2643 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
SacII CC_GC'GG
Cuts at: 2021 2021
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SexAI A'CCwGG_T
Cuts at: 2232 2232
Size: 6121
SfcI C'TryA_G
Cuts at: 826 1045 3469 4604 5285 5476 826
Size: 219 2424 1135 681 191 1471
Fragments arranged by size:
2424 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 2465 1700
Size: 765 5356
SmaI CCC'GGG
Cuts at: 1872 3448 1872
Size: 1576 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 3178 4045 5
Size: 47 3126 867 2081
Fragments arranged by size:
3126 2081 867 47
StuI AGG'CCT
Cuts at: 1517 3265 1517
Size: 1748 4373
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2190 3150 3401 3560 513
Size: 552 939 111 75 960 251 159 3074
Fragments arranged by size:
3074 960 939 552 251 159 111 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3088 3323 4148 4165 4318 4503 4948
Size: 1255 1508 235 825 17 153 185 445
Cuts at: 4948 325
Size: 1498
Fragments arranged by size:
1508 1498 1255 825 445 235 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2870 3213 4367
Size: 274 80 33 51 1443 893 343 1154
Cuts at: 4367 6079 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1154 893 343 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 2928 2960 6081 6113 2681
Size: 32 215 32 3121 32 2689
Fragments arranged by size:
3121 2689 215 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 3446 1870
Size: 1576 4545
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI