Restriction map for
pFN21AE0056
(Linear) (Six-base) MAPSORT of: KIEE0056.seq Check: 3514 from: 1 to: 1416
KIEE0056.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 167 328 1416
Size: 167 161 1088
Fragments arranged by size:
1088 167 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 831 1416
Size: 831 585
AleI CACnn'nnGTG
Cuts at: 0 329 1416
Size: 329 1087
AlwNI CAG_nnn'CTG
Cuts at: 0 977 1416
Size: 977 439
ApoI r'AATT_y
Cuts at: 0 287 1190 1416
Size: 287 903 226
Fragments arranged by size:
903 287 226
BamHI G'GATC_C
Cuts at: 0 387 1130 1416
Size: 387 743 286
Fragments arranged by size:
743 387 286
BanI G'GyrC_C
Cuts at: 0 231 1416
Size: 231 1185
BglII A'GATC_T
Cuts at: 0 764 1416
Size: 764 652
Bme1580I G_kGCm'C
Cuts at: 0 302 1416
Size: 302 1114
BmgBI CAC'GTC
Cuts at: 0 618 701 1416
Size: 618 83 715
Fragments arranged by size:
715 618 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 1101 1416
Size: 1101 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1034 1416
Size: 541 493 382
Fragments arranged by size:
541 493 382
BsaI GGTCTCn'nnnn_
Cuts at: 0 609 1416
Size: 609 807
BsaAI yAC'GTr
Cuts at: 0 600 1416
Size: 600 816
BsaHI Gr'CG_yC
Cuts at: 0 644 1416
Size: 644 772
BsaWI w'CCGG_w
Cuts at: 0 390 1061 1416
Size: 390 671 355
Fragments arranged by size:
671 390 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 923 953 1416
Size: 253 30 640 30 463
Fragments arranged by size:
640 463 253 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 1416
Size: 340 1076
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 471 1416
Size: 354 117 945
Fragments arranged by size:
945 354 117
BsiHKAI G_wGCw'C
Cuts at: 0 22 308 319 425 1416
Size: 22 286 11 106 991
Fragments arranged by size:
991 286 106 22 11
BsmI GAATG_Cn'
Cuts at: 0 49 1416
Size: 49 1367
BsmBI CGTCTCn'nnnn_
Cuts at: 0 573 1416
Size: 573 843
Bsp1286I G_dGCh'C
Cuts at: 0 22 302 308 319 425 1416
Size: 22 280 6 11 106 991
Fragments arranged by size:
991 280 106 22 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 328 1416
Size: 167 161 1088
Fragments arranged by size:
1088 167 161
BsrDI GCAATG_nn'
Cuts at: 0 479 1416
Size: 479 937
BsrFI r'CCGG_y
Cuts at: 0 460 1416
Size: 460 956
BsrGI T'GTAC_A
Cuts at: 0 865 1416
Size: 865 551
BssSI C'ACGA_G
Cuts at: 0 301 1416
Size: 301 1115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 213 231 1416
Size: 213 18 1185
Fragments arranged by size:
1185 213 18
BstBI TT'CG_AA
Cuts at: 0 921 1416
Size: 921 495
BstYI r'GATC_y
Cuts at: 0 275 387 764 1130 1416
Size: 275 112 377 366 286
Fragments arranged by size:
377 366 286 275 112
Bsu36I CC'TnA_GG
Cuts at: 0 123 1416
Size: 123 1293
BtgI C'CryG_G
Cuts at: 0 13 110 1220 1416
Size: 13 97 1110 196
Fragments arranged by size:
1110 196 97 13
DraI TTT'AAA
Cuts at: 0 285 1416
Size: 285 1131
DraIII CAC_nnn'GTG
Cuts at: 0 231 312 1416
Size: 231 81 1104
Fragments arranged by size:
1104 231 81
EaeI y'GGCC_r
Cuts at: 0 164 852 1416
Size: 164 688 564
Fragments arranged by size:
688 564 164
EarI CTCTTCn'nnn_
Cuts at: 0 1293 1416
Size: 1293 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 54 1416
Size: 54 1362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 831 1034 1416
Size: 541 290 203 382
Fragments arranged by size:
541 382 290 203
EcoNI CCTnn'n_nnAGG
Cuts at: 0 189 1416
Size: 189 1227
EcoO109I rG'GnC_Cy
Cuts at: 0 119 261 509 516 1416
Size: 119 142 248 7 900
Fragments arranged by size:
900 248 142 119 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 253 285 763 795 1339 1371 1416
Size: 253 32 478 32 544 32 45
Fragments arranged by size:
544 478 253 45 32 32 32
MscI TGG'CCA
Cuts at: 0 166 854 1416
Size: 166 688 562
Fragments arranged by size:
688 562 166
MslI CAynn'nnrTG
Cuts at: 0 329 818 1416
Size: 329 489 598
Fragments arranged by size:
598 489 329
MspA1I CmG'CkG
Cuts at: 0 15 1416
Size: 15 1401
NcoI C'CATG_G
Cuts at: 0 110 1416
Size: 110 1306
NruI TCG'CGA
Cuts at: 0 413 1416
Size: 413 1003
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 1416
Size: 732 684
PpuMI rG'GwC_Cy
Cuts at: 0 261 516 1416
Size: 261 255 900
Fragments arranged by size:
900 261 255
SacII CC_GC'GG
Cuts at: 0 16 1416
Size: 16 1400
SexAI A'CCwGG_T
Cuts at: 0 227 1416
Size: 227 1189
SgrAI Cr'CCGG_yG
Cuts at: 0 460 1416
Size: 460 956
SspI AAT'ATT
Cuts at: 0 1173 1416
Size: 1173 243
StuI AGG'CCT
Cuts at: 0 1260 1416
Size: 1260 156
StyI C'CwwG_G
Cuts at: 0 110 185 1145 1396 1416
Size: 110 75 960 251 20
Fragments arranged by size:
960 251 110 75 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1083 1318 1416
Size: 1083 235 98
Fragments arranged by size:
1083 235 98
TatI w'GTAC_w
Cuts at: 0 865 1208 1416
Size: 865 343 208
Fragments arranged by size:
865 343 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 676 708 923 955 1416
Size: 676 32 215 32 461
Fragments arranged by size:
676 461 215 32 32
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI BamHI BanI BglII
Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI DraI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I Hin4I MscI MslI MspA1I NcoI NruI PflMI
PpuMI SacII SexAI SgrAI SspI StuI StyI TaqII
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I
BpuEI BsaBI BseYI BsiEI BsiWI BspEI BspHI BsrBI
BssHII BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI