(Circular) (Six-base) MAPSORT of: pFN21AE0052.seq Check: 505 from: 1 to: 7966 pFN21AE0052 7966 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5776 278 Size: 53 83 186 936 4240 2468 Fragments arranged by size: 4240 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4315 4740 5309 1937 Size: 2378 425 569 4594 Fragments arranged by size: 4594 2378 569 425 Acc65I G'GTAC_C Cuts at: 5287 5287 Size: 7966 AclI AA'CG_TT Cuts at: 1563 4491 6093 6466 1563 Size: 2928 1602 373 3063 Fragments arranged by size: 3063 2928 1602 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2021 2927 2982 3029 3528 5183 6029 7413 Size: 389 906 55 47 499 1655 846 1384 Cuts at: 7413 7681 1632 Size: 268 1917 Fragments arranged by size: 1917 1655 1384 906 846 499 389 268 55 47 AflII C'TTAA_G Cuts at: 820 1017 3926 820 Size: 197 2909 4860 Fragments arranged by size: 4860 2909 197 AflIII A'CryG_T Cuts at: 1203 2222 6865 7842 1203 Size: 1019 4643 977 1327 Fragments arranged by size: 4643 1327 1019 977 AgeI A'CCGG_T Cuts at: 6797 6797 Size: 7966 AhdI GACnn_n'nnGTC Cuts at: 6695 6695 Size: 7966 AleI CACnn'nnGTG Cuts at: 1166 2758 4748 1166 Size: 1592 1990 4384 Fragments arranged by size: 4384 1990 1592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7966 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 2555 Size: 32 7934 AlwNI CAG_nnn'CTG Cuts at: 5709 7281 5709 Size: 1572 6394 ApaLI G'TGCA_C Cuts at: 6023 7179 6023 Size: 1156 6810 ApoI r'AATT_y Cuts at: 1493 2432 2660 3190 3743 3817 4028 4341 5157 Size: 939 228 530 553 74 211 313 816 Cuts at: 5157 5203 5275 5501 5653 7762 1493 Size: 46 72 226 152 2109 1697 Fragments arranged by size: 2109 1697 939 816 553 530 313 228 226 211 152 74 72 46 AseI AT'TA_AT Cuts at: 160 1032 2888 3312 4260 4575 6520 160 Size: 872 1856 424 948 315 1945 1606 Fragments arranged by size: 1945 1856 1606 948 872 424 315 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7966 AvaI C'yCGr_G Cuts at: 1870 1943 2177 5291 1870 Size: 73 234 3114 4545 Fragments arranged by size: 4545 3114 234 73 BamHI G'GATC_C Cuts at: 5296 5663 5296 Size: 367 7599 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2181 5287 6742 618 Size: 325 217 627 394 3106 1455 1842 Fragments arranged by size: 3106 1842 1455 627 394 325 217 BanII G_rGCy'C Cuts at: 1391 2145 4919 5285 1391 Size: 754 2774 366 4072 Fragments arranged by size: 4072 2774 754 366 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2585 3049 3452 3653 4440 5181 928 Size: 971 686 464 403 201 787 741 3713 Fragments arranged by size: 3713 971 787 741 686 464 403 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6157 6191 1903 Size: 34 4220 34 3678 Fragments arranged by size: 4220 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5858 7068 5858 Size: 1210 6756 BclI T'GATC_A Cuts at: 1548 1548 Size: 7966 BfrBI ATG'CAT Cuts at: 2885 3206 4361 4646 2885 Size: 321 1155 285 6205 Fragments arranged by size: 6205 1155 321 285 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6577 136 Size: 107 122 71 631 605 4905 1525 Fragments arranged by size: 4905 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3910 4562 7961 3910 Size: 652 3399 3915 Fragments arranged by size: 3915 3399 652 BlpI GC'TnA_GC Cuts at: 5383 5383 Size: 7966 Bme1580I G_kGCm'C Cuts at: 1790 2934 6027 7183 1790 Size: 1144 3093 1156 2573 Fragments arranged by size: 3093 2573 1156 1144 BmgBI CAC'GTC Cuts at: 1318 2101 1318 Size: 783 7183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 5697 6655 Size: 416 53 83 399 24 2459 1821 958 Cuts at: 6655 442 Size: 1753 Fragments arranged by size: 2459 1821 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7966 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2613 2613 Size: 7966 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2398 2840 4872 4941 5023 6626 1239 Size: 1159 442 2032 69 82 1603 2579 Fragments arranged by size: 2579 2032 1603 1159 442 82 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3994 6091 6956 7254 3994 Size: 2097 865 298 4706 Fragments arranged by size: 4706 2097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2416 6629 882 Size: 617 917 4213 2219 Fragments arranged by size: 4213 2219 917 617 BsaAI yAC'GTr Cuts at: 493 1204 5757 493 Size: 711 4553 2702 Fragments arranged by size: 4553 2702 711 BsaBI GATnn'nnATC Cuts at: 3345 3372 7960 3345 Size: 27 4588 3351 Fragments arranged by size: 4588 3351 27 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 4015 5773 6155 Size: 53 83 186 936 600 1882 1758 382 Cuts at: 6155 6790 275 Size: 635 1451 Fragments arranged by size: 1882 1758 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4800 6399 6797 7071 7218 7629 4800 Size: 1599 398 274 147 411 5137 Fragments arranged by size: 5137 1599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2034 2064 1178 Size: 30 165 30 631 30 7080 Fragments arranged by size: 7080 631 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2120 2153 4697 4802 4861 1186 Size: 901 33 33 2544 105 59 4291 Fragments arranged by size: 4291 2544 901 105 59 33 33 BseYI C'CCAG_C Cuts at: 4937 7169 7665 4937 Size: 2232 496 5238 Fragments arranged by size: 5238 2232 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7393 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5416 6177 7205 1540 Size: 275 186 3415 761 1028 2301 Fragments arranged by size: 3415 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2389 3834 4919 6027 6112 7183 2145 Size: 244 1445 1085 1108 85 1071 2928 Fragments arranged by size: 2928 1445 1108 1085 1071 244 85 BsmI GAATG_Cn' Cuts at: 2887 3544 4363 4810 5477 5570 2887 Size: 657 819 447 667 93 5283 Fragments arranged by size: 5283 819 667 657 447 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2389 2934 3834 4919 5285 6027 Size: 399 355 244 545 900 1085 366 742 Cuts at: 6027 6112 7183 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1085 1071 900 742 545 399 366 355 244 85 BspEI T'CCGG_A Cuts at: 4800 7629 4800 Size: 2829 5137 BspHI T'CATG_A Cuts at: 5855 7585 5855 Size: 1730 6236 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 5321 844 Size: 1190 3287 3489 Fragments arranged by size: 3489 3287 1190 BsrBI CCG'CTC Cuts at: 1394 1823 5853 1394 Size: 429 4030 3507 Fragments arranged by size: 4030 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4510 6461 6635 65 Size: 1183 3262 1951 174 1396 Fragments arranged by size: 3262 1951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6610 6797 1700 Size: 110 4800 187 2869 Fragments arranged by size: 4800 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7966 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7966 BssSI C'ACGA_G Cuts at: 6026 7038 6026 Size: 1012 6954 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7966 BstBI TT'CG_AA Cuts at: 6770 6770 Size: 7966 BstEII G'GTnAC_C Cuts at: 1184 3278 1184 Size: 2094 5872 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2065 Size: 7966 BstYI r'GATC_y Cuts at: 1972 2062 3910 4562 5296 5663 6048 6065 7517 Size: 90 1848 652 734 367 385 17 1452 Cuts at: 7517 7603 7615 7961 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1848 1452 734 652 385 367 346 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2759 3737 4907 513 Size: 552 939 755 978 1170 3572 Fragments arranged by size: 3572 1170 978 939 755 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2824 3076 3213 487 Size: 683 56 1598 252 137 5240 Fragments arranged by size: 5240 1598 683 252 137 56 BtsI GCAGTG_nn' Cuts at: 2155 5482 6276 6296 2155 Size: 3327 794 20 3825 Fragments arranged by size: 3825 3327 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7966 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7931 DraI TTT'AAA Cuts at: 2692 5270 2692 Size: 2578 5388 DraIII CAC_nnn'GTG Cuts at: 3678 3678 Size: 7966 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3928 6973 809 Size: 507 2612 3045 1802 Fragments arranged by size: 3045 2612 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 5413 6302 Size: 54 1425 50 275 18 177 3406 889 Cuts at: 6302 7931 8 Size: 1629 43 Fragments arranged by size: 3406 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5413 1537 Size: 275 3601 4090 Fragments arranged by size: 4090 3601 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2559 2733 2856 4110 4907 5020 5896 Size: 756 450 174 123 1254 797 113 876 Cuts at: 5896 1353 Size: 3423 Fragments arranged by size: 3423 1254 876 797 756 450 174 123 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3602 6553 6927 7073 387 Size: 1064 138 2013 2951 374 146 1280 Fragments arranged by size: 2951 2013 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2143 4917 2143 Size: 2774 5192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2398 2840 2927 2982 3029 3528 Size: 393 389 377 442 87 55 47 499 Cuts at: 3528 4872 4941 5023 5183 6029 6626 7413 7681 Size: 1344 69 82 160 846 597 787 268 Cuts at: 7681 1239 Size: 1524 Fragments arranged by size: 1524 1344 846 787 597 499 442 393 389 377 268 160 87 82 69 55 47 EcoO109I rG'GnC_Cy Cuts at: 2042 4934 2042 Size: 2892 5074 EcoRI G'AATT_C Cuts at: 2660 3190 5275 5653 2660 Size: 530 2085 378 4973 Fragments arranged by size: 4973 2085 530 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4495 4495 Size: 7966 FspI TGC'GCA Cuts at: 6472 6472 Size: 7966 HaeII r_GCGC'y Cuts at: 1678 7113 1678 Size: 5435 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2102 2134 4886 Size: 32 135 32 462 32 36 32 2752 Cuts at: 4886 4918 6607 6639 6681 6713 1373 Size: 32 1689 32 42 32 2626 Fragments arranged by size: 2752 2626 1689 462 135 42 36 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3578 5310 5556 669 Size: 988 281 1640 1732 246 3079 Fragments arranged by size: 3079 1732 1640 988 281 246 HindIII A'AGCT_T Cuts at: 748 3848 748 Size: 3100 4866 HpaI GTT'AAC Cuts at: 5556 5556 Size: 7966 KpnI G_GTAC'C Cuts at: 5291 5291 Size: 7966 MfeI C'AATT_G Cuts at: 5565 5565 Size: 7966 MluI A'CGCG_T Cuts at: 7842 7842 Size: 7966 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2804 3468 4085 7080 7264 1305 Size: 1499 664 617 2995 184 2007 Fragments arranged by size: 2995 2007 1499 664 617 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5967 Fragments arranged by size: 5967 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 2758 2937 3138 4748 5924 6283 Size: 648 1216 376 179 201 1610 1176 359 Cuts at: 6283 6442 518 Size: 159 2042 Fragments arranged by size: 2042 1610 1216 1176 648 376 359 201 179 159 MspA1I CmG'CkG Cuts at: 1606 2860 3337 3635 4764 5382 6059 7207 7452 Size: 1254 477 298 1129 618 677 1148 245 Cuts at: 7452 1606 Size: 2120 Fragments arranged by size: 2120 1254 1148 1129 677 618 477 298 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3737 513 Size: 552 939 1733 4742 Fragments arranged by size: 4742 1733 939 552 NdeI CA'TA_TG Cuts at: 387 2938 2979 3040 387 Size: 2551 41 61 5313 Fragments arranged by size: 5313 2551 61 41 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7966 NotI GC'GGCC_GC Cuts at: 5413 5413 Size: 7966 NruI TCG'CGA Cuts at: 5107 5107 Size: 7966 NsiI A_TGCA'T Cuts at: 2887 3208 4363 4648 2887 Size: 321 1155 285 6205 Fragments arranged by size: 6205 1155 321 285 NspI r_CATG'y Cuts at: 1132 1229 2226 4646 4739 5324 6869 1132 Size: 97 997 2420 93 585 1545 2229 Fragments arranged by size: 2420 2229 1545 997 585 97 93 PasI CC'CwG_GG Cuts at: 1348 2058 1348 Size: 710 7256 PciI A'CATG_T Cuts at: 2222 6865 2222 Size: 4643 3323 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7966 PmeI GTTT'AAAC Cuts at: 5270 5270 Size: 7966 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2140 6035 6067 7548 7580 2108 Size: 32 3895 32 1481 32 2494 Fragments arranged by size: 3895 2494 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 4934 2042 Size: 2892 5074 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7966 PsiI TTA'TAA Cuts at: 5536 5536 Size: 7966 PstI C_TGCA'G Cuts at: 830 2150 4174 5318 830 Size: 1320 2024 1144 3478 Fragments arranged by size: 3478 2024 1320 1144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7966 PvuII CAG'CTG Cuts at: 2860 3337 3635 2860 Size: 477 298 7191 Fragments arranged by size: 7191 477 298 SacI G_AGCT'C Cuts at: 2145 4919 2145 Size: 2774 5192 SalI G'TCGA_C Cuts at: 1936 5308 1936 Size: 3372 4594 SapI GCTCTTCn'nnn_ Cuts at: 4907 4907 Size: 7966 SbfI CC_TGCA'GG Cuts at: 5318 5318 Size: 7966 ScaI AGT'ACT Cuts at: 6214 7926 6214 Size: 1712 6254 SexAI A'CCwGG_T Cuts at: 3892 3892 Size: 7966 SfcI C'TryA_G Cuts at: 826 1045 2146 2788 3597 3621 3792 4170 4965 Size: 219 1101 642 809 24 171 378 795 Cuts at: 4965 5314 6449 7130 7321 826 Size: 349 1135 681 191 1471 Fragments arranged by size: 1471 1135 1101 809 795 681 642 378 349 219 191 171 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7966 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7966 SmaI CCC'GGG Cuts at: 1872 2179 5293 1872 Size: 307 3114 4545 Fragments arranged by size: 4545 3114 307 SmlI C'TyrA_G Cuts at: 820 1017 1943 3926 4009 6070 6971 7233 820 Size: 197 926 1983 83 2061 901 262 1553 Fragments arranged by size: 2061 1983 1553 926 901 262 197 83 SnaBI TAC'GTA Cuts at: 493 5757 493 Size: 5264 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7966 SphI G_CATG'C Cuts at: 1132 5324 1132 Size: 4192 3774 SspI AAT'ATT Cuts at: 5 52 4628 5890 5 Size: 47 4576 1262 2081 Fragments arranged by size: 4576 2081 1262 47 StuI AGG'CCT Cuts at: 1517 5012 1517 Size: 3495 4471 StyI C'CwwG_G Cuts at: 513 1065 2004 3128 3737 3859 5405 513 Size: 552 939 1124 609 122 1546 3074 Fragments arranged by size: 3074 1546 1124 939 609 552 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 3799 5993 6010 6163 6348 6793 Size: 1255 491 1728 2194 17 153 185 445 Cuts at: 6793 325 Size: 1498 Fragments arranged by size: 2194 1728 1498 1255 491 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6212 7924 96 Size: 274 80 33 51 1443 4235 1712 138 Fragments arranged by size: 4235 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3283 3315 7926 7958 3283 Size: 32 4611 32 3291 Fragments arranged by size: 4611 3291 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2968 1316 Size: 1652 6314 XbaI T'CTAG_A Cuts at: 4417 5302 4417 Size: 885 7081 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2519 4579 2065 Size: 454 2060 5452 Fragments arranged by size: 5452 2060 454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7966 XmaI C'CCGG_G Cuts at: 1870 2177 5291 1870 Size: 307 3114 4545 Fragments arranged by size: 4545 3114 307 XmnI GAAnn'nnTTC Cuts at: 2664 4850 6095 2664 Size: 2186 1245 4535 Fragments arranged by size: 4535 2186 1245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5774 276 Size: 53 83 186 936 4240 2468 Fragments arranged by size: 4240 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI