Restriction map for
pFN21AE0051
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccIII AclI AflII AflIII
Aor51HI AscI AseI AsnI AviII
AvrII BbeI BbrPI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRV FseI FspI HaeII HpaI
KasI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 964 GGTACC G^GTACC
AccI 385 GTCTAC GT^[AC][GT]AC
AcsI 1444 GAATTC [GA]^AATT[CT]
AgeI 676 ACCGGT A^CCGGT
Alw44I 2625 GTGCAC G^TGCAC
ApaI 2879 GGGCCC GGGCC^C
ApaLI 2625 GTGCAC G^TGCAC
ApoI 1444 GAATTC [GA]^AATT[CT]
Asp718I 964 GGTACC G^GTACC
BfaI 2974 CTAG C^TAG
BglII 2915 AGATCT A^GATCT
BsaWI 676 ACCGGT [AT]^CCGG[AT]
BsmBI 1762 GAGACG CGTCTC(1/5)
BsmI 987 GAATGC GAATGC(1/-1)
BspHI 1865 TCATGA T^CATGA
DraIII 2892 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1444 GAATTC G^AATTC
EcoT22I 207 ATGCAT ATGCA^T
HindIII 1002 AAGCTT A^AGCTT
KpnI 964 GGTACC GGTAC^C
KspI 867 CCGCGG CCGC^GG
MaeI 2974 CTAG C^TAG
NsiI 207 ATGCAT ATGCA^T
PflMI 2926 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 676 ACCGGT A^CCGGT
PvuI 1947 CGATCG CGAT^CG
RcaI 1865 TCATGA T^CATGA
SacI 118 GAGCTC GAGCT^C
SacII 867 CCGCGG CCGC^GG
SapI 2496 GCTCTTC GCTCTTC(1/4)
SgrAI 675 CACCGGTG C[GA]^CCGG[CT]G
StuI 653 AGGCCT AGG^CCT
Van91I 2926 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AhdI 397 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1468 GACAGAAAGTC
AocI 1552 CCTGAGG CC^T[ACGT]AGG
2350 CCTGAGG
AspEI 397 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1468 GACAGAAAGTC
AspHI 118 GAGCTC G[AT]GC[AT]^C
2625 GTGCAC
BamHI 1853 GGATCC G^GATCC
2979 GGATCC
BbvCI 127 GCTGAGG CCTCAGC(-5/-2)
2575 GCTGAGG
BcgI 2011 CGACGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
209 GCATTTCCTTCG
BsaBI 1095 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1863 GATCATGATC
BsiEI 1566 CGACCG CG[GA][CT]^CG
1947 CGATCG
BsiHKAI 118 GAGCTC G[AT]GC[AT]^C
2625 GTGCAC
BsrBI 8 CCGCTC CCGCTC(-3/-3)
325 GAGCGG
BstEII 2391 GGTGACC G^GT[ACGT]ACC
2712 GGTGACC
BstPI 2391 GGTGACC G^GT[ACGT]ACC
2712 GGTGACC
Bsu36I 1552 CCTGAGG CC^T[ACGT]AGG
2350 CCTGAGG
DrdI 540 GACAGGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2659 GACTGTGAGGTC
Eam1105I 397 GACCGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1468 GACAGAAAGTC
EarI 2497 CTCTTC CTCTTC(1/4)
244 GAAGAG
Eco81I 1552 CCTGAGG CC^T[ACGT]AGG
2350 CCTGAGG
EcoNI 1383 CCTCTCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2130 CCTGAAGAAGG
EcoO65I 2391 GGTGACC G^GT[ACGT]ACC
2712 GGTGACC
Ksp632I 2497 CTCTTC CTCTTC(1/4)
244 GAAGAG
MaeII 2013 ACGT A^CGT
2303 ACGT
MamI 1095 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1863 GATCATGATC
MseI 86 TTAA T^TAA
887 TTAA
NaeI 646 GCCGGC GCC^GGC
1907 GCCGGC
NgoMIV 646 GCCGGC G^CCGGC
1907 GCCGGC
SmlI 2911 CTCAAG C^T[CT][GA]AG
2950 CTCAAG
SphI 205 GCATGC GCATG^C
1793 GCATGC
Tru9I 86 TTAA T^TAA
887 TTAA
Tsp509I 1445 AATT ^AATT
1914 AATT
XcmI 299 CCAATGCCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
671 CCACCACCGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Asp700I AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI Tth111I TthHB8I XhoII
XmaCI XmaI XmnI