(Circular) (Six-base) MAPSORT of: pFN21AE0051.seq Check: 1466 from: 1 to: 7708 pFN21AE0051 7708 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2963 2963 Size: 7708 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5518 278 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2391 5051 1937 Size: 454 2660 4594 Fragments arranged by size: 4594 2660 454 Acc65I G'GTAC_C Cuts at: 2969 5029 2969 Size: 2060 5648 AclI AA'CG_TT Cuts at: 1563 5835 6208 1563 Size: 4272 373 3063 Fragments arranged by size: 4272 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 4157 5771 7155 7423 1632 Size: 749 1776 1614 1384 268 1917 Fragments arranged by size: 1917 1776 1614 1384 749 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7511 AflIII A'CryG_T Cuts at: 1203 6607 7584 1203 Size: 5404 977 1327 Fragments arranged by size: 5404 1327 977 AgeI A'CCGG_T Cuts at: 2681 6539 2681 Size: 3858 3850 AhdI GACnn_n'nnGTC Cuts at: 2407 3478 6437 2407 Size: 1071 2959 3678 Fragments arranged by size: 3678 2959 1071 AleI CACnn'nnGTG Cuts at: 1166 2684 3347 3377 3605 4178 1166 Size: 1518 663 30 228 573 4696 Fragments arranged by size: 4696 1518 663 573 228 30 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 1184 Size: 1245 6463 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4736 4768 4736 Size: 32 7676 AlwNI CAG_nnn'CTG Cuts at: 2761 4497 4768 5451 7023 2761 Size: 1736 271 683 1572 3446 Fragments arranged by size: 3446 1736 1572 683 271 ApaI G_GGCC'C Cuts at: 4888 4888 Size: 7708 ApaLI G'TGCA_C Cuts at: 4630 5765 6921 4630 Size: 1135 1156 5417 Fragments arranged by size: 5417 1156 1135 ApoI r'AATT_y Cuts at: 1493 3449 5017 5243 5395 7504 1493 Size: 1956 1568 226 152 2109 1697 Fragments arranged by size: 2109 1956 1697 1568 226 152 AseI AT'TA_AT Cuts at: 160 1032 6262 160 Size: 872 5230 1606 Fragments arranged by size: 5230 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 AvaI C'yCGr_G Cuts at: 1870 1943 2430 2595 2632 3468 3516 3603 3702 Size: 73 487 165 37 836 48 87 99 Cuts at: 3702 4685 5033 1870 Size: 983 348 4545 Fragments arranged by size: 4545 983 836 487 348 165 99 87 73 48 37 BamHI G'GATC_C Cuts at: 3858 4984 5038 5405 3858 Size: 1126 54 367 6161 Fragments arranged by size: 6161 1126 367 54 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2636 2690 2969 3065 3551 Size: 325 217 627 849 54 279 96 486 Cuts at: 3551 3596 3607 5029 6484 618 Size: 45 11 1422 1455 1842 Fragments arranged by size: 1842 1455 1422 849 627 486 325 279 217 96 54 45 11 BanII G_rGCy'C Cuts at: 1391 2127 2583 2864 3833 4047 4410 4475 4485 Size: 736 456 281 969 214 363 65 10 Cuts at: 4485 4692 4811 4888 4944 5027 1391 Size: 207 119 77 56 83 4072 Fragments arranged by size: 4072 969 736 456 363 281 214 207 119 83 77 65 56 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2184 2927 4666 928 Size: 971 251 34 743 1739 3970 Fragments arranged by size: 3970 1739 971 743 251 34 BbvCI CC'TCA_GC Cuts at: 2133 4581 2133 Size: 2448 5260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2203 2237 4005 4039 5899 5933 1903 Size: 34 266 34 1768 34 1860 34 3678 Fragments arranged by size: 3678 1860 1768 266 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5600 6810 5600 Size: 1210 6498 BclI T'GATC_A Cuts at: 1548 3648 3867 3873 1548 Size: 2100 219 6 5383 Fragments arranged by size: 5383 2100 219 6 BfrBI ATG'CAT Cuts at: 2214 2214 Size: 7708 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2354 2657 Size: 107 122 71 631 605 337 345 303 Cuts at: 2657 3211 6319 136 Size: 554 3108 1525 Fragments arranged by size: 3108 1525 631 605 554 345 337 303 122 107 71 BglII A'GATC_T Cuts at: 4920 7703 4920 Size: 2783 4925 BlpI GC'TnA_GC Cuts at: 5125 5125 Size: 7708 Bme1580I G_kGCm'C Cuts at: 1790 2639 2693 3259 3556 3665 3896 4634 4888 Size: 849 54 566 297 109 231 738 254 Cuts at: 4888 5769 6925 1790 Size: 881 1156 2573 Fragments arranged by size: 2573 1156 881 849 738 566 297 254 231 109 54 BmgBI CAC'GTC Cuts at: 1318 4019 1318 Size: 2701 5007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3888 4894 5439 Size: 416 53 83 399 24 2471 1006 545 Cuts at: 5439 6397 442 Size: 958 1753 Fragments arranged by size: 2471 1753 1006 958 545 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7708 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 7708 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 3159 3479 3779 6368 1239 Size: 884 1036 320 300 2589 2579 Fragments arranged by size: 2589 2579 1036 884 320 300 Bpu10I CC'TnA_GC Cuts at: 2133 4581 2133 Size: 2448 5260 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4901 4940 5833 6698 6996 4901 Size: 39 893 865 298 5613 Fragments arranged by size: 5613 893 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 2246 2706 3017 6371 882 Size: 617 558 189 460 311 3354 2219 Fragments arranged by size: 3354 2219 617 558 460 311 189 BsaAI yAC'GTr Cuts at: 493 1204 5499 493 Size: 711 4295 2702 Fragments arranged by size: 4295 2702 711 BsaBI GATnn'nnATC Cuts at: 3104 3872 7702 3104 Size: 768 3830 3110 Fragments arranged by size: 3830 3110 768 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3564 3852 4116 4125 Size: 53 83 186 936 2031 288 264 9 Cuts at: 4125 5515 5897 6532 275 Size: 1390 382 635 1451 Fragments arranged by size: 2031 1451 1390 936 635 382 288 264 186 83 53 9 BsaWI w'CCGG_w Cuts at: 2681 6141 6539 6813 6960 7371 2681 Size: 3460 398 274 147 411 3018 Fragments arranged by size: 3460 3018 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3157 3187 4208 4238 1178 Size: 30 165 30 1754 30 1021 30 4648 Fragments arranged by size: 4648 1754 1021 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 3262 3956 4262 1186 Size: 1003 1073 694 306 4632 Fragments arranged by size: 4632 1073 1003 694 306 BseYI C'CCAG_C Cuts at: 3502 4411 4804 6911 7407 3502 Size: 909 393 2107 496 3803 Fragments arranged by size: 3803 2107 909 496 393 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3019 4394 4560 4817 1163 Size: 573 1283 1375 166 257 4054 Fragments arranged by size: 4054 1375 1283 573 257 166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 3955 5158 5919 6947 1540 Size: 275 186 1573 381 1203 761 1028 2301 Fragments arranged by size: 2301 1573 1203 1028 761 381 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 4634 5769 5854 6925 2127 Size: 2507 1135 85 1071 2910 Fragments arranged by size: 2910 2507 1135 1071 85 BsmI GAATG_Cn' Cuts at: 2998 5219 5312 2998 Size: 2221 93 5394 Fragments arranged by size: 5394 2221 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3761 3761 Size: 7708 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2583 2639 2693 2864 3259 3556 Size: 399 337 456 56 54 171 395 297 Cuts at: 3556 3665 3833 3896 4047 4410 4475 4485 4634 Size: 109 168 63 151 363 65 10 149 Cuts at: 4634 4692 4811 4888 4944 5027 5769 5854 6925 Size: 58 119 77 56 83 742 85 1071 Cuts at: 6925 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 456 399 395 363 337 297 171 168 151 149 119 109 85 83 77 65 63 58 56 56 54 10 BspEI T'CCGG_A Cuts at: 7371 7371 Size: 7708 BspHI T'CATG_A Cuts at: 3870 5597 7327 3870 Size: 1727 1730 4251 Fragments arranged by size: 4251 1730 1727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 3993 4083 5063 844 Size: 2119 1030 90 980 3489 Fragments arranged by size: 3489 2119 1030 980 90 BsrBI CCG'CTC Cuts at: 1394 1823 2015 2332 5595 1394 Size: 429 192 317 3263 3507 Fragments arranged by size: 3507 3263 429 317 192 BsrDI GCAATG_nn' Cuts at: 65 1248 2298 2643 3271 3900 4155 4741 6203 Size: 1183 1050 345 628 629 255 586 1462 Cuts at: 6203 6377 65 Size: 174 1396 Fragments arranged by size: 1462 1396 1183 1050 629 628 586 345 255 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 2681 3158 3622 3912 6352 6539 Size: 110 841 30 477 464 290 2440 187 Cuts at: 6539 1700 Size: 2869 Fragments arranged by size: 2869 2440 841 477 464 290 187 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7708 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7708 BssSI C'ACGA_G Cuts at: 5768 6780 5768 Size: 1012 6696 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7708 BstBI TT'CG_AA Cuts at: 6512 6512 Size: 7708 BstEII G'GTnAC_C Cuts at: 1184 4396 4717 1184 Size: 3212 321 4175 Fragments arranged by size: 4175 3212 321 BstXI CCAn_nnnn'nTGG Cuts at: 2686 2959 3349 3757 4066 4423 4699 2686 Size: 273 390 408 309 357 276 5695 Fragments arranged by size: 5695 408 390 357 309 276 273 BstYI r'GATC_y Cuts at: 1972 2706 3858 4166 4920 4984 5038 5405 5790 Size: 734 1152 308 754 64 54 367 385 Cuts at: 5790 5807 7259 7345 7357 7703 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1152 754 734 385 367 346 308 86 64 54 17 12 Bsu36I CC'TnA_GG Cuts at: 3558 4356 3558 Size: 798 6910 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2067 2854 2872 2901 4161 Size: 552 939 24 39 787 18 29 1260 Cuts at: 4161 4179 4548 4698 513 Size: 18 369 150 3523 Fragments arranged by size: 3523 1260 939 787 552 369 150 39 29 24 18 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6969 Fragments arranged by size: 6969 683 56 BtsI GCAGTG_nn' Cuts at: 2259 4173 4634 4925 5224 6018 6038 2259 Size: 1914 461 291 299 794 20 3929 Fragments arranged by size: 3929 1914 794 461 299 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7673 DraI TTT'AAA Cuts at: 5012 5012 Size: 7708 DraIII CAC_nnn'GTG Cuts at: 4902 4902 Size: 7708 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2551 4670 6715 809 Size: 507 1235 2119 2045 1802 Fragments arranged by size: 2119 2045 1802 1235 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2673 2833 Size: 54 1425 50 275 18 375 468 160 Cuts at: 2833 3212 3243 3667 3696 3948 4182 5155 6044 Size: 379 31 424 29 252 234 973 889 Cuts at: 6044 7673 8 Size: 1629 43 Fragments arranged by size: 1629 1425 973 889 468 424 379 375 275 252 234 160 54 50 43 31 29 18 EagI C'GGCC_G Cuts at: 1537 1812 5155 1537 Size: 275 3343 4090 Fragments arranged by size: 4090 3343 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 4508 5638 1353 Size: 891 2264 1130 3423 Fragments arranged by size: 3423 2264 1130 891 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3419 6295 6669 6815 387 Size: 1064 138 1830 2876 374 146 1280 Fragments arranged by size: 2876 1830 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2125 2125 Size: 7708 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2381 3159 3479 3779 4157 5771 Size: 393 491 258 778 320 300 378 1614 Cuts at: 5771 6368 7155 7423 1239 Size: 597 787 268 1524 Fragments arranged by size: 1614 1524 787 778 597 491 393 378 320 300 268 258 EcoNI CCTnn'n_nnAGG Cuts at: 3392 4139 3392 Size: 747 6961 EcoO109I rG'GnC_Cy Cuts at: 2356 4360 4681 4885 2356 Size: 2004 321 204 5179 Fragments arranged by size: 5179 2004 321 204 EcoRI G'AATT_C Cuts at: 3449 5017 5395 3449 Size: 1568 378 5762 Fragments arranged by size: 5762 1568 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 7708 FspI TGC'GCA Cuts at: 6214 6214 Size: 7708 HaeII r_GCGC'y Cuts at: 1678 6855 1678 Size: 5177 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2413 2425 2445 2543 Size: 32 135 32 821 20 12 20 98 Cuts at: 2543 2575 3464 3496 4208 4240 4748 4780 6349 Size: 32 889 32 712 32 508 32 1569 Cuts at: 6349 6381 6423 6455 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 889 821 712 508 135 98 42 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2530 2605 4600 4855 5052 5298 Size: 988 281 592 75 1995 255 197 246 Cuts at: 5298 669 Size: 3079 Fragments arranged by size: 3079 1995 988 592 281 255 246 197 75 HindIII A'AGCT_T Cuts at: 748 3007 748 Size: 2259 5449 HpaI GTT'AAC Cuts at: 5298 5298 Size: 7708 KpnI G_GTAC'C Cuts at: 2973 5033 2973 Size: 2060 5648 MfeI C'AATT_G Cuts at: 5307 5307 Size: 7708 MluI A'CGCG_T Cuts at: 7584 7584 Size: 7708 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2957 3078 4329 6822 7006 1305 Size: 1652 121 1251 2493 184 2007 Fragments arranged by size: 2493 2007 1652 1251 184 121 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 2835 3245 3698 4184 Size: 54 1425 343 843 160 410 453 486 Cuts at: 4184 10 Size: 3534 Fragments arranged by size: 3534 1425 843 486 453 410 343 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 2807 3347 3377 3605 3644 4100 Size: 648 1518 123 540 30 228 39 456 Cuts at: 4100 4160 4178 4697 4727 5666 6025 6184 518 Size: 60 18 519 30 939 359 159 2042 Fragments arranged by size: 2042 1518 939 648 540 519 456 359 228 159 123 60 39 30 30 18 MspA1I CmG'CkG Cuts at: 1606 2874 3576 3841 4003 4340 4378 4559 4612 Size: 1268 702 265 162 337 38 181 53 Cuts at: 4612 5124 5801 6949 7194 1606 Size: 512 677 1148 245 2120 Fragments arranged by size: 2120 1268 1148 702 677 512 337 265 245 181 162 53 38 NaeI GCC'GGC Cuts at: 2653 3914 2653 Size: 1261 6447 NcoI C'CATG_G Cuts at: 513 1065 2004 2854 4161 4698 513 Size: 552 939 850 1307 537 3523 Fragments arranged by size: 3523 1307 939 850 552 537 NdeI CA'TA_TG Cuts at: 387 387 Size: 7708 NgoMIV G'CCGG_C Cuts at: 2651 3912 2651 Size: 1261 6447 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7708 NotI GC'GGCC_GC Cuts at: 5155 5155 Size: 7708 NsiI A_TGCA'T Cuts at: 2216 2216 Size: 7708 NspI r_CATG'y Cuts at: 1132 1229 2214 2626 3802 5066 6611 1132 Size: 97 985 412 1176 1264 1545 2229 Fragments arranged by size: 2229 1545 1264 1176 985 412 97 PasI CC'CwG_GG Cuts at: 1348 2444 2965 3028 4345 4423 4785 1348 Size: 1096 521 63 1317 78 362 4271 Fragments arranged by size: 4271 1317 1096 521 362 78 63 PciI A'CATG_T Cuts at: 6607 6607 Size: 7708 PflMI CCAn_nnn'nTGG Cuts at: 1108 4937 1108 Size: 3829 3879 PfoI T'CCnGG_A Cuts at: 3735 3756 3735 Size: 21 7687 PmeI GTTT'AAAC Cuts at: 5012 5012 Size: 7708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5777 5809 7290 7322 5777 Size: 32 1481 32 6163 Fragments arranged by size: 6163 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7708 PsiI TTA'TAA Cuts at: 5278 5278 Size: 7708 PspOMI G'GGCC_C Cuts at: 4884 4884 Size: 7708 PstI C_TGCA'G Cuts at: 830 2488 2944 4080 5060 830 Size: 1658 456 1136 980 3478 Fragments arranged by size: 3478 1658 1136 980 456 PvuI CG_AT'CG Cuts at: 2001 3955 2001 Size: 1954 5754 PvuII CAG'CTG Cuts at: 3841 4378 4559 4612 3841 Size: 537 181 53 6937 Fragments arranged by size: 6937 537 181 53 SacI G_AGCT'C Cuts at: 2127 2127 Size: 7708 SacII CC_GC'GG Cuts at: 2875 2875 Size: 7708 SalI G'TCGA_C Cuts at: 1936 5050 1936 Size: 3114 4594 SapI GCTCTTCn'nnn_ Cuts at: 4508 4508 Size: 7708 SbfI CC_TGCA'GG Cuts at: 5060 5060 Size: 7708 ScaI AGT'ACT Cuts at: 5956 7668 5956 Size: 1712 5996 SfcI C'TryA_G Cuts at: 826 1045 2484 2940 4076 4603 5056 6191 6872 Size: 219 1439 456 1136 527 453 1135 681 Cuts at: 6872 7063 826 Size: 191 1471 Fragments arranged by size: 1471 1439 1136 1135 681 527 456 453 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7708 SgrAI Cr'CCGG_yG Cuts at: 1700 2681 1700 Size: 981 6727 SmaI CCC'GGG Cuts at: 1872 3518 3605 3704 5035 1872 Size: 1646 87 99 1331 4545 Fragments arranged by size: 4545 1646 1331 99 87 SmlI C'TyrA_G Cuts at: 820 1017 1943 4916 4955 5812 6713 6975 820 Size: 197 926 2973 39 857 901 262 1553 Fragments arranged by size: 2973 1553 926 901 857 262 197 39 SnaBI TAC'GTA Cuts at: 493 5499 493 Size: 5006 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7708 SphI G_CATG'C Cuts at: 1132 2214 3802 5066 1132 Size: 1082 1588 1264 3774 Fragments arranged by size: 3774 1588 1264 1082 SspI AAT'ATT Cuts at: 5 52 5632 5 Size: 47 5580 2081 Fragments arranged by size: 5580 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 6565 StyI C'CwwG_G Cuts at: 513 1065 2004 2661 2787 2854 2907 3276 4161 Size: 552 939 657 126 67 53 369 885 Cuts at: 4161 4476 4698 5147 513 Size: 315 222 449 3074 Fragments arranged by size: 3074 939 885 657 552 449 369 315 222 126 67 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 4375 4415 4526 4661 4777 4913 Size: 1255 2196 599 40 111 135 116 136 Cuts at: 4913 5735 5752 5905 6090 6535 325 Size: 822 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 822 599 445 185 153 136 135 116 111 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5954 7666 96 Size: 274 80 33 51 1443 3977 1712 138 Fragments arranged by size: 3977 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3157 3189 7668 7700 3157 Size: 32 4479 32 3165 Fragments arranged by size: 4479 3165 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2438 2477 2849 1316 Size: 1122 39 372 6175 Fragments arranged by size: 6175 1122 372 39 XbaI T'CTAG_A Cuts at: 5044 5044 Size: 7708 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2683 2311 Size: 372 7336 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7708 XmaI C'CCGG_G Cuts at: 1870 3516 3603 3702 5033 1870 Size: 1646 87 99 1331 4545 Fragments arranged by size: 4545 1646 1331 99 87 XmnI GAAnn'nnTTC Cuts at: 2934 3146 3365 3449 4280 5837 2934 Size: 212 219 84 831 1557 4805 Fragments arranged by size: 4805 1557 831 219 212 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5516 276 Size: 53 83 186 936 3982 2468 Fragments arranged by size: 3982 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI