Restriction map for
pFN21AE0051
(Linear) (Six-base) MAPSORT of: KIEE0051.seq Check: 6858 from: 1 to: 3003
KIEE0051.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 958 3003
Size: 958 2045
AccI GT'mk_AC
Cuts at: 0 386 3003
Size: 386 2617
Acc65I G'GTAC_C
Cuts at: 0 964 3003
Size: 964 2039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2152 3003
Size: 376 1776 851
Fragments arranged by size:
1776 851 376
AgeI A'CCGG_T
Cuts at: 0 676 3003
Size: 676 2327
AhdI GACnn_n'nnGTC
Cuts at: 0 402 1473 3003
Size: 402 1071 1530
Fragments arranged by size:
1530 1071 402
AleI CACnn'nnGTG
Cuts at: 0 679 1342 1372 1600 2173 3003
Size: 679 663 30 228 573 830
Fragments arranged by size:
830 679 663 573 228 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 3003
Size: 424 2579
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2731 2763 3003
Size: 2731 32 240
Fragments arranged by size:
2731 240 32
AlwNI CAG_nnn'CTG
Cuts at: 0 756 2492 2763 3003
Size: 756 1736 271 240
Fragments arranged by size:
1736 756 271 240
ApaI G_GGCC'C
Cuts at: 0 2883 3003
Size: 2883 120
ApaLI G'TGCA_C
Cuts at: 0 2625 3003
Size: 2625 378
ApoI r'AATT_y
Cuts at: 0 1444 3003
Size: 1444 1559
AvaI C'yCGr_G
Cuts at: 0 425 590 627 1463 1511 1598 1697 2680
Size: 425 165 37 836 48 87 99 983
Cuts at: 2680 3003
Size: 323
Fragments arranged by size:
983 836 425 323 165 99 87 48
37
BamHI G'GATC_C
Cuts at: 0 1853 2979 3003
Size: 1853 1126 24
Fragments arranged by size:
1853 1126 24
BanI G'GyrC_C
Cuts at: 0 631 685 964 1060 1546 1591 1602 3003
Size: 631 54 279 96 486 45 11 1401
Fragments arranged by size:
1401 631 486 279 96 54 45 11
BanII G_rGCy'C
Cuts at: 0 122 578 859 1828 2042 2405 2470 2480
Size: 122 456 281 969 214 363 65 10
Cuts at: 2480 2687 2806 2883 2939 3003
Size: 207 119 77 56 64
Fragments arranged by size:
969 456 363 281 214 207 122 119
77 65 64 56 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 179 922 2661 3003
Size: 145 34 743 1739 342
Fragments arranged by size:
1739 743 342 145 34
BbvCI CC'TCA_GC
Cuts at: 0 128 2576 3003
Size: 128 2448 427
Fragments arranged by size:
2448 427 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 232 2000 2034 3003
Size: 198 34 1768 34 969
Fragments arranged by size:
1768 969 198 34 34
BclI T'GATC_A
Cuts at: 0 1643 1862 1868 3003
Size: 1643 219 6 1135
Fragments arranged by size:
1643 1135 219 6
BfrBI ATG'CAT
Cuts at: 0 209 3003
Size: 209 2794
BglI GCCn_nnn'nGGC
Cuts at: 0 349 652 1206 3003
Size: 349 303 554 1797
Fragments arranged by size:
1797 554 349 303
BglII A'GATC_T
Cuts at: 0 2915 3003
Size: 2915 88
Bme1580I G_kGCm'C
Cuts at: 0 634 688 1254 1551 1660 1891 2629 2883
Size: 634 54 566 297 109 231 738 254
Cuts at: 2883 3003
Size: 120
Fragments arranged by size:
738 634 566 297 254 231 120 109
54
BmgBI CAC'GTC
Cuts at: 0 2014 3003
Size: 2014 989
BmrI ACTGGGnnnn_n'
Cuts at: 0 1883 2889 3003
Size: 1883 1006 114
Fragments arranged by size:
1883 1006 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 795 3003
Size: 795 2208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1154 1474 1774 3003
Size: 118 1036 320 300 1229
Fragments arranged by size:
1229 1036 320 300 118
Bpu10I CC'TnA_GC
Cuts at: 0 128 2576 3003
Size: 128 2448 427
Fragments arranged by size:
2448 427 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2896 2935 3003
Size: 2896 39 68
Fragments arranged by size:
2896 68 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 241 701 1012 3003
Size: 52 189 460 311 1991
Fragments arranged by size:
1991 460 311 189 52
BsaBI GATnn'nnATC
Cuts at: 0 1099 1867 3003
Size: 1099 768 1136
Fragments arranged by size:
1136 1099 768
BsaHI Gr'CG_yC
Cuts at: 0 1559 1847 2111 2120 3003
Size: 1559 288 264 9 883
Fragments arranged by size:
1559 883 288 264 9
BsaWI w'CCGG_w
Cuts at: 0 676 3003
Size: 676 2327
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1152 1182 2203 2233 3003
Size: 1152 30 1021 30 770
Fragments arranged by size:
1152 1021 770 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1257 1951 2257 3003
Size: 184 1073 694 306 746
Fragments arranged by size:
1073 746 694 306 184
BseYI C'CCAG_C
Cuts at: 0 1497 2406 2799 3003
Size: 1497 909 393 204
Fragments arranged by size:
1497 909 393 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 2389 2555 2812 3003
Size: 1014 1375 166 257 191
Fragments arranged by size:
1375 1014 257 191 166
BsiEI CG_ry'CG
Cuts at: 0 1569 1950 3003
Size: 1569 381 1053
Fragments arranged by size:
1569 1053 381
BsiHKAI G_wGCw'C
Cuts at: 0 122 2629 3003
Size: 122 2507 374
Fragments arranged by size:
2507 374 122
BsmI GAATG_Cn'
Cuts at: 0 993 3003
Size: 993 2010
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1756 3003
Size: 1756 1247
Bsp1286I G_dGCh'C
Cuts at: 0 122 578 634 688 859 1254 1551 1660
Size: 122 456 56 54 171 395 297 109
Cuts at: 1660 1828 1891 2042 2405 2470 2480 2629 2687
Size: 168 63 151 363 65 10 149 58
Cuts at: 2687 2806 2883 2939 3003
Size: 119 77 56 64
Fragments arranged by size:
456 395 363 297 171 168 151 149
122 119 109 77 65 64 63 58
56 56 54 10
BspHI T'CATG_A
Cuts at: 0 1865 3003
Size: 1865 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 958 1988 2078 3003
Size: 958 1030 90 925
Fragments arranged by size:
1030 958 925 90
BsrBI CCG'CTC
Cuts at: 0 10 327 3003
Size: 10 317 2676
Fragments arranged by size:
2676 317 10
BsrDI GCAATG_nn'
Cuts at: 0 293 638 1266 1895 2150 2736 3003
Size: 293 345 628 629 255 586 267
Fragments arranged by size:
629 628 586 345 293 267 255
BsrFI r'CCGG_y
Cuts at: 0 646 676 1153 1617 1907 3003
Size: 646 30 477 464 290 1096
Fragments arranged by size:
1096 646 477 464 290 30
BstEII G'GTnAC_C
Cuts at: 0 2391 2712 3003
Size: 2391 321 291
Fragments arranged by size:
2391 321 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 954 1344 1752 2061 2418 2694 3003
Size: 681 273 390 408 309 357 276 309
Fragments arranged by size:
681 408 390 357 309 309 276 273
BstYI r'GATC_y
Cuts at: 0 701 1853 2161 2915 2979 3003
Size: 701 1152 308 754 64 24
Fragments arranged by size:
1152 754 701 308 64 24
Bsu36I CC'TnA_GG
Cuts at: 0 1553 2351 3003
Size: 1553 798 652
Fragments arranged by size:
1553 798 652
BtgI C'CryG_G
Cuts at: 0 23 62 849 867 896 2156 2174 2543
Size: 23 39 787 18 29 1260 18 369
Cuts at: 2543 2693 3003
Size: 150 310
Fragments arranged by size:
1260 787 369 310 150 39 29 23
18 18
BtsI GCAGTG_nn'
Cuts at: 0 254 2168 2629 2920 3003
Size: 254 1914 461 291 83
Fragments arranged by size:
1914 461 291 254 83
DraIII CAC_nnn'GTG
Cuts at: 0 2897 3003
Size: 2897 106
DrdI GACnn_nn'nnGTC
Cuts at: 0 546 2665 3003
Size: 546 2119 338
Fragments arranged by size:
2119 546 338
EaeI y'GGCC_r
Cuts at: 0 200 668 828 1207 1238 1662 1691 1943
Size: 200 468 160 379 31 424 29 252
Cuts at: 1943 2177 3003
Size: 234 826
Fragments arranged by size:
826 468 424 379 252 234 200 160
31 29
EarI CTCTTCn'nnn_
Cuts at: 0 239 2503 3003
Size: 239 2264 500
Fragments arranged by size:
2264 500 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1414 3003
Size: 1414 1589
EcoICRI GAG'CTC
Cuts at: 0 120 3003
Size: 120 2883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 376 1154 1474 1774 2152 3003
Size: 118 258 778 320 300 378 851
Fragments arranged by size:
851 778 378 320 300 258 118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1387 2134 3003
Size: 1387 747 869
Fragments arranged by size:
1387 869 747
EcoO109I rG'GnC_Cy
Cuts at: 0 351 2355 2676 2880 3003
Size: 351 2004 321 204 123
Fragments arranged by size:
2004 351 321 204 123
EcoRI G'AATT_C
Cuts at: 0 1444 3003
Size: 1444 1559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 3003
Size: 759 2244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 388 408 420 440 538 570 1459 1491
Size: 388 20 12 20 98 32 889 32
Cuts at: 1491 2203 2235 2743 2775 3003
Size: 712 32 508 32 228
Fragments arranged by size:
889 712 508 388 228 98 32 32
32 32 20 20 12
HincII GTy'rAC
Cuts at: 0 525 600 2595 2850 3003
Size: 525 75 1995 255 153
Fragments arranged by size:
1995 525 255 153 75
HindIII A'AGCT_T
Cuts at: 0 1002 3003
Size: 1002 2001
KpnI G_GTAC'C
Cuts at: 0 968 3003
Size: 968 2035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1073 2324 3003
Size: 952 121 1251 679
Fragments arranged by size:
1251 952 679 121
MscI TGG'CCA
Cuts at: 0 670 830 1240 1693 2179 3003
Size: 670 160 410 453 486 824
Fragments arranged by size:
824 670 486 453 410 160
MslI CAynn'nnrTG
Cuts at: 0 679 802 1342 1372 1600 1639 2095 2155
Size: 679 123 540 30 228 39 456 60
Cuts at: 2155 2173 2692 2722 3003
Size: 18 519 30 281
Fragments arranged by size:
679 540 519 456 281 228 123 60
39 30 30 18
MspA1I CmG'CkG
Cuts at: 0 869 1571 1836 1998 2335 2373 2554 2607
Size: 869 702 265 162 337 38 181 53
Cuts at: 2607 3003
Size: 396
Fragments arranged by size:
869 702 396 337 265 181 162 53
38
NaeI GCC'GGC
Cuts at: 0 648 1909 3003
Size: 648 1261 1094
Fragments arranged by size:
1261 1094 648
NcoI C'CATG_G
Cuts at: 0 849 2156 2693 3003
Size: 849 1307 537 310
Fragments arranged by size:
1307 849 537 310
NgoMIV G'CCGG_C
Cuts at: 0 646 1907 3003
Size: 646 1261 1096
Fragments arranged by size:
1261 1096 646
NsiI A_TGCA'T
Cuts at: 0 211 3003
Size: 211 2792
NspI r_CATG'y
Cuts at: 0 209 621 1797 3003
Size: 209 412 1176 1206
Fragments arranged by size:
1206 1176 412 209
PasI CC'CwG_GG
Cuts at: 0 439 960 1023 2340 2418 2780 3003
Size: 439 521 63 1317 78 362 223
Fragments arranged by size:
1317 521 439 362 223 78 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 2932 3003
Size: 2932 71
PfoI T'CCnGG_A
Cuts at: 0 1730 1751 3003
Size: 1730 21 1252
Fragments arranged by size:
1730 1252 21
PspOMI G'GGCC_C
Cuts at: 0 2879 3003
Size: 2879 124
PstI C_TGCA'G
Cuts at: 0 483 939 2075 3003
Size: 483 456 1136 928
Fragments arranged by size:
1136 928 483 456
PvuI CG_AT'CG
Cuts at: 0 1950 3003
Size: 1950 1053
PvuII CAG'CTG
Cuts at: 0 1836 2373 2554 2607 3003
Size: 1836 537 181 53 396
Fragments arranged by size:
1836 537 396 181 53
SacI G_AGCT'C
Cuts at: 0 122 3003
Size: 122 2881
SacII CC_GC'GG
Cuts at: 0 870 3003
Size: 870 2133
SapI GCTCTTCn'nnn_
Cuts at: 0 2503 3003
Size: 2503 500
SfcI C'TryA_G
Cuts at: 0 479 935 2071 2598 3003
Size: 479 456 1136 527 405
Fragments arranged by size:
1136 527 479 456 405
SgrAI Cr'CCGG_yG
Cuts at: 0 676 3003
Size: 676 2327
SmaI CCC'GGG
Cuts at: 0 1513 1600 1699 3003
Size: 1513 87 99 1304
Fragments arranged by size:
1513 1304 99 87
SmlI C'TyrA_G
Cuts at: 0 2911 2950 3003
Size: 2911 39 53
Fragments arranged by size:
2911 53 39
SphI G_CATG'C
Cuts at: 0 209 1797 3003
Size: 209 1588 1206
Fragments arranged by size:
1588 1206 209
StuI AGG'CCT
Cuts at: 0 655 3003
Size: 655 2348
StyI C'CwwG_G
Cuts at: 0 656 782 849 902 1271 2156 2471 2693
Size: 656 126 67 53 369 885 315 222
Cuts at: 2693 3003
Size: 310
Fragments arranged by size:
885 656 369 315 310 222 126 67
53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1771 2370 2410 2521 2656 2772 2908 3003
Size: 1771 599 40 111 135 116 136 95
Fragments arranged by size:
1771 599 136 135 116 111 95 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1152 1184 3003
Size: 1152 32 1819
Fragments arranged by size:
1819 1152 32
Tth111I GACn'n_nGTC
Cuts at: 0 433 472 844 3003
Size: 433 39 372 2159
Fragments arranged by size:
2159 433 372 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 678 3003
Size: 306 372 2325
Fragments arranged by size:
2325 372 306
XmaI C'CCGG_G
Cuts at: 0 1511 1598 1697 3003
Size: 1511 87 99 1306
Fragments arranged by size:
1511 1306 99 87
XmnI GAAnn'nnTTC
Cuts at: 0 929 1141 1360 1444 2275 3003
Size: 929 212 219 84 831 728
Fragments arranged by size:
929 831 728 219 212 84
Enzymes that do cut:
AarI AccI Acc65I AcuI AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PflMI PfoI PspOMI PstI PvuI PvuII SacI SacII
SapI SfcI SgrAI SmaI SmlI SphI StuI StyI
TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AscI AseI AsiSI
AvrII BaeI BbeI BciVI BlpI BmtI BsaAI BsiWI
BspEI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoRV FseI FspI FspAI
HaeII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI
SspI SwaI TatI XbaI XhoI ZraI