(Circular) (Six-base) MAPSORT of: pFN21AB7276.seq Check: 4179 from: 1 to: 5851 pFN21AB7276 5851 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2698 2951 3914 5298 5566 1632 Size: 806 260 253 963 1384 268 1917 Fragments arranged by size: 1917 1384 963 806 268 260 253 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 4580 4580 Size: 5851 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2356 3594 5166 2356 Size: 1238 1572 3041 Fragments arranged by size: 3041 1572 1238 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 2458 2738 2860 3143 3160 3386 3538 5647 Size: 965 280 122 283 17 226 152 2109 Cuts at: 5647 1493 Size: 1697 Fragments arranged by size: 2109 1697 965 283 280 226 152 122 17 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2418 3176 1870 Size: 73 475 758 4545 Fragments arranged by size: 4545 758 475 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 4627 618 Size: 325 217 627 1385 1455 1842 Fragments arranged by size: 1842 1455 1385 627 325 217 BanII G_rGCy'C Cuts at: 1391 3170 1391 Size: 1779 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BfrBI ATG'CAT Cuts at: 2192 2192 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4462 136 Size: 107 122 71 631 605 2790 1525 Fragments arranged by size: 2790 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 3912 5068 1790 Size: 2122 1156 2573 Fragments arranged by size: 2573 2122 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4511 1239 Size: 3272 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2893 3642 493 Size: 711 1689 749 2702 Fragments arranged by size: 2702 1689 749 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5626 Fragments arranged by size: 5626 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2786 1186 Size: 1600 4251 BseYI C'CCAG_C Cuts at: 5054 5550 5054 Size: 496 5355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3060 1163 Size: 573 1324 3954 Fragments arranged by size: 3954 1324 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3912 3997 5068 3912 Size: 85 1071 4695 Fragments arranged by size: 4695 1071 85 BsmI GAATG_Cn' Cuts at: 2479 3362 3455 2479 Size: 883 93 4875 Fragments arranged by size: 4875 883 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3170 3912 3997 5068 1391 Size: 399 1380 742 85 1071 2174 Fragments arranged by size: 2174 1380 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3206 844 Size: 2362 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2650 2964 4346 4520 65 Size: 1183 1402 314 1382 174 1396 Fragments arranged by size: 1402 1396 1382 1183 314 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 2879 794 Size: 2085 3766 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstYI r'GATC_y Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846 Size: 1209 367 385 17 1452 86 12 346 Cuts at: 5846 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5299 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3367 4161 4181 3043 Size: 324 794 20 4713 Fragments arranged by size: 4713 794 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 3298 4187 Size: 54 1425 50 275 18 501 967 889 Cuts at: 4187 5816 8 Size: 1629 43 Fragments arranged by size: 1629 1425 967 889 501 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2763 3781 1353 Size: 1410 1018 3423 Fragments arranged by size: 3423 1410 1018 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2698 2951 3914 4511 5298 5566 Size: 393 806 260 253 963 597 787 268 Cuts at: 5566 1239 Size: 1524 Fragments arranged by size: 1524 963 806 787 597 393 268 260 253 EcoNI CCTnn'n_nnAGG Cuts at: 2692 2692 Size: 5851 EcoRI G'AATT_C Cuts at: 3143 3160 3538 3143 Size: 17 378 5456 Fragments arranged by size: 5456 378 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2468 2542 2468 Size: 74 5777 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 4998 1678 Size: 3320 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 4492 4524 4566 Size: 32 135 32 827 32 2061 32 42 Cuts at: 4566 4598 1373 Size: 32 2626 Fragments arranged by size: 2626 2061 827 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3092 3195 3441 669 Size: 988 281 1154 103 246 3079 Fragments arranged by size: 3079 1154 988 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2127 4965 5149 1305 Size: 822 2838 184 2007 Fragments arranged by size: 2838 2007 822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 10 Size: 54 1425 343 501 3528 Fragments arranged by size: 3528 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3809 4168 4327 518 Size: 648 2643 359 159 2042 Fragments arranged by size: 2643 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3267 3944 5092 5337 1606 Size: 1661 677 1148 245 2120 Fragments arranged by size: 2120 1661 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5299 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NsiI A_TGCA'T Cuts at: 2194 2194 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2075 3209 4754 1132 Size: 97 846 1134 1545 2229 Fragments arranged by size: 2229 1545 1134 846 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PmlI CAC'GTG Cuts at: 2893 2893 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PstI C_TGCA'G Cuts at: 830 3203 830 Size: 2373 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 3199 4334 5015 5206 826 Size: 219 2154 1135 681 191 1471 Fragments arranged by size: 2154 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2418 3955 4856 5118 820 Size: 197 926 475 1537 901 262 1553 Fragments arranged by size: 1553 1537 926 901 475 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 2075 3209 1132 Size: 943 1134 3774 Fragments arranged by size: 3774 1134 943 SspI AAT'ATT Cuts at: 5 52 2122 2365 3775 5 Size: 47 2070 243 1410 2081 Fragments arranged by size: 2081 2070 1410 243 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2382 2633 3290 513 Size: 552 1317 251 657 3074 Fragments arranged by size: 3074 1317 657 552 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3878 3895 4048 4233 4678 325 Size: 1255 2298 17 153 185 445 1498 Fragments arranged by size: 2298 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3114 4097 5809 Size: 274 80 33 51 1443 1137 983 1712 Cuts at: 5809 96 Size: 138 Fragments arranged by size: 1712 1443 1137 983 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3028 3187 3028 Size: 159 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2844 2844 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 2418 1943 Size: 475 5376 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI