(Circular) (Six-base) MAPSORT of: pFN21AB6636.seq Check: 8898 from: 1 to: 6028 pFN21AB6636 6028 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3371 1937 Size: 1434 4594 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 2469 2561 2658 3027 4091 5475 5743 Size: 758 79 92 97 369 1064 1384 268 Cuts at: 5743 1632 Size: 1917 Fragments arranged by size: 1917 1384 1064 758 369 268 97 92 79 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 4927 5904 1203 Size: 3724 977 1327 Fragments arranged by size: 3724 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 2740 2742 1166 Size: 1574 2 4452 Fragments arranged by size: 4452 1574 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 2117 3771 5343 2117 Size: 1654 1572 2802 Fragments arranged by size: 2802 1654 1572 ApaLI G'TGCA_C Cuts at: 3276 4085 5241 3276 Size: 809 1156 4063 Fragments arranged by size: 4063 1156 809 ApoI r'AATT_y Cuts at: 1493 2415 2986 3066 3337 3563 3715 5824 1493 Size: 922 571 80 271 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 571 271 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 3353 1870 Size: 73 1410 4545 Fragments arranged by size: 4545 1410 73 BamHI G'GATC_C Cuts at: 2374 3358 3725 2374 Size: 984 367 4677 Fragments arranged by size: 4677 984 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3349 4804 618 Size: 325 217 627 1562 1455 1842 Fragments arranged by size: 1842 1562 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3347 1391 Size: 1956 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 2664 3032 928 Size: 971 711 54 368 3924 Fragments arranged by size: 3924 971 711 368 54 BbvCI CC'TCA_GC Cuts at: 2633 2633 Size: 6028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2926 3920 5130 2926 Size: 994 1210 3824 Fragments arranged by size: 3824 1210 994 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4639 136 Size: 107 122 71 631 605 2967 1525 Fragments arranged by size: 2967 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6023 6023 Size: 6028 BlpI GC'TnA_GC Cuts at: 2763 3445 2763 Size: 682 5346 Bme1580I G_kGCm'C Cuts at: 1790 3280 4089 5245 1790 Size: 1490 809 1156 2573 Fragments arranged by size: 2573 1490 1156 809 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3759 4717 442 Size: 416 53 83 399 24 2342 958 1753 Fragments arranged by size: 2342 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2139 2400 2447 4688 1239 Size: 900 261 47 2241 2579 Fragments arranged by size: 2579 2241 900 261 47 Bpu10I CC'TnA_GC Cuts at: 2633 2633 Size: 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2832 4153 5018 5316 2832 Size: 1321 865 298 3544 Fragments arranged by size: 3544 1321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4691 882 Size: 617 3192 2219 Fragments arranged by size: 3192 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3835 4217 4852 275 Size: 53 83 186 936 2302 382 635 1451 Fragments arranged by size: 2302 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4461 4859 5133 5280 5691 4461 Size: 398 274 147 411 4798 Fragments arranged by size: 4798 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 2373 2403 1178 Size: 30 165 30 662 30 278 30 4803 Fragments arranged by size: 4803 662 278 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2762 1186 Size: 1576 4452 BseYI C'CCAG_C Cuts at: 5231 5727 5231 Size: 496 5532 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5455 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 4239 5267 1540 Size: 275 186 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3280 4089 4174 5245 3280 Size: 809 85 1071 4063 Fragments arranged by size: 4063 1071 809 85 BsmI GAATG_Cn' Cuts at: 3539 3632 3539 Size: 93 5935 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3280 3347 4089 4174 5245 1391 Size: 399 1490 67 742 85 1071 2174 Fragments arranged by size: 2174 1490 1071 742 399 85 67 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3383 844 Size: 2539 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3915 1394 Size: 429 2092 3507 Fragments arranged by size: 3507 2092 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 4523 4697 65 Size: 1183 1701 1574 174 1396 Fragments arranged by size: 1701 1574 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4672 4859 1700 Size: 110 2862 187 2869 Fragments arranged by size: 2869 2862 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 4088 5100 4088 Size: 1012 5016 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6028 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstYI r'GATC_y Cuts at: 1972 2374 3358 3725 4110 4127 5579 5665 5677 Size: 402 984 367 385 17 1452 86 12 Cuts at: 5677 6023 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 984 402 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 3174 3544 4338 4358 3174 Size: 370 794 20 4844 Fragments arranged by size: 4844 794 370 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5993 DraI TTT'AAA Cuts at: 2961 3332 2961 Size: 371 5657 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3475 4364 5993 Size: 54 1425 50 275 18 1645 889 1629 Cuts at: 5993 8 Size: 43 Fragments arranged by size: 1645 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3475 1537 Size: 275 1663 4090 Fragments arranged by size: 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 2197 3126 3156 3958 1353 Size: 823 21 929 30 802 3423 Fragments arranged by size: 3423 929 823 802 30 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3224 4615 4989 5135 387 Size: 1064 138 1635 1391 374 146 1280 Fragments arranged by size: 1635 1391 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2139 2390 2400 2447 2469 2561 2658 Size: 393 507 251 10 47 22 92 97 Cuts at: 2658 3027 4091 4688 5475 5743 1239 Size: 369 1064 597 787 268 1524 Fragments arranged by size: 1524 1064 787 597 507 393 369 268 251 97 92 47 22 10 EcoRI G'AATT_C Cuts at: 3337 3715 3337 Size: 378 5650 EcoRV GAT'ATC Cuts at: 2302 2302 Size: 6028 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 5175 1678 Size: 3497 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 4669 4701 4743 Size: 32 135 32 896 32 2169 32 42 Cuts at: 4743 4775 1373 Size: 32 2626 Fragments arranged by size: 2626 2169 896 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3319 3372 3618 669 Size: 988 281 1381 53 246 3079 Fragments arranged by size: 3079 1381 988 281 246 53 HindIII A'AGCT_T Cuts at: 748 2829 2927 748 Size: 2081 98 3849 Fragments arranged by size: 3849 2081 98 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 2349 2721 3627 2349 Size: 372 906 4750 Fragments arranged by size: 4750 906 372 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5142 5326 1305 Size: 3837 184 2007 Fragments arranged by size: 3837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4206 Fragments arranged by size: 4206 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2642 2740 2742 3986 4345 4504 518 Size: 648 1476 98 2 1244 359 159 2042 Fragments arranged by size: 2042 1476 1244 648 359 159 98 2 MspA1I CmG'CkG Cuts at: 1606 2921 3444 4121 5269 5514 1606 Size: 1315 523 677 1148 245 2120 Fragments arranged by size: 2120 1315 1148 677 523 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 2231 3005 3038 3386 4931 1132 Size: 97 1002 774 33 348 1545 2229 Fragments arranged by size: 2229 1545 1002 774 348 97 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6028 PciI A'CATG_T Cuts at: 4927 4927 Size: 6028 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6028 PfoI T'CCnGG_A Cuts at: 2377 2377 Size: 6028 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3252 3284 4097 4129 5610 5642 3252 Size: 32 813 32 1481 32 3638 Fragments arranged by size: 3638 1481 813 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 3598 3598 Size: 6028 PstI C_TGCA'G Cuts at: 830 2113 2670 3380 830 Size: 1283 557 710 3478 Fragments arranged by size: 3478 1283 710 557 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SapI GCTCTTCn'nnn_ Cuts at: 3126 3126 Size: 6028 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 4276 5988 4276 Size: 1712 4316 SfcI C'TryA_G Cuts at: 826 1045 2109 2666 3376 4511 5192 5383 826 Size: 219 1064 557 710 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 710 681 557 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6028 SmaI CCC'GGG Cuts at: 1872 3355 1872 Size: 1483 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2847 4132 5033 5295 820 Size: 197 926 904 1285 901 262 1553 Fragments arranged by size: 1553 1285 926 904 901 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3005 3386 1132 Size: 1873 381 3774 Fragments arranged by size: 3774 1873 381 SspI AAT'ATT Cuts at: 5 52 2865 3952 5 Size: 47 2813 1087 2081 Fragments arranged by size: 2813 2081 1087 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6028 StyI C'CwwG_G Cuts at: 513 1065 2004 2093 3467 513 Size: 552 939 89 1374 3074 Fragments arranged by size: 3074 1374 939 552 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4055 4072 4225 4410 4855 325 Size: 1255 2475 17 153 185 445 1498 Fragments arranged by size: 2475 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2152 2709 4274 Size: 274 80 33 51 1443 175 557 1565 Cuts at: 4274 5986 96 Size: 1712 138 Fragments arranged by size: 1712 1565 1443 557 274 175 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 5988 6020 2159 Size: 32 3797 32 2167 Fragments arranged by size: 3797 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6028 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 3353 1870 Size: 1483 4545 XmnI GAAnn'nnTTC Cuts at: 4157 4157 Size: 6028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI