(Circular) (Six-base) MAPSORT of: pFN21AE0796.seq Check: 3797 from: 1 to: 5977 pFN21AE0796 5977 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4040 5424 5692 1632 Size: 2408 1384 268 1917 Fragments arranged by size: 2408 1917 1384 268 AfeI AGC'GCT Cuts at: 2723 2723 Size: 5977 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 3720 5292 3720 Size: 1572 4405 ApaI G_GGCC'C Cuts at: 3229 3229 Size: 5977 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2599 2632 2599 Size: 33 5944 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 3298 4753 618 Size: 325 217 627 411 1100 1455 1842 Fragments arranged by size: 1842 1455 1100 627 411 325 217 BanII G_rGCy'C Cuts at: 1391 3229 3263 3296 1391 Size: 1838 34 33 4072 Fragments arranged by size: 4072 1838 34 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2683 3258 928 Size: 971 784 575 3647 Fragments arranged by size: 3647 971 784 575 BbvCI CC'TCA_GC Cuts at: 3090 3090 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2262 2905 3229 4038 5194 1790 Size: 472 643 324 809 1156 2573 Fragments arranged by size: 2573 1156 809 643 472 324 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2908 3708 4666 Size: 416 53 83 399 24 1491 800 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1491 958 800 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2529 2544 2896 3000 3032 4637 1239 Size: 1290 15 352 104 32 1605 2579 Fragments arranged by size: 2579 1605 1290 352 104 32 15 Bpu10I CC'TnA_GC Cuts at: 3090 3090 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4102 4967 5265 4102 Size: 865 298 4814 Fragments arranged by size: 4814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2282 2312 2918 2948 1178 Size: 30 165 30 879 30 606 30 4207 Fragments arranged by size: 4207 879 606 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 3161 1186 Size: 912 1063 4002 Fragments arranged by size: 4002 1063 912 BseYI C'CCAG_C Cuts at: 2811 5180 5676 2811 Size: 2369 496 3112 Fragments arranged by size: 3112 2369 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3023 1163 Size: 573 1287 4117 Fragments arranged by size: 4117 1287 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 4188 5216 1540 Size: 275 186 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4038 4123 5194 4038 Size: 85 1071 4821 Fragments arranged by size: 4821 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 3214 2285 Size: 929 5048 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2905 3229 3263 3296 4038 4123 Size: 399 472 643 324 34 33 742 85 Cuts at: 4123 5194 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 643 472 399 324 85 34 33 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3332 844 Size: 2488 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2499 4472 4646 65 Size: 1183 1251 1973 174 1396 Fragments arranged by size: 1973 1396 1251 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 2145 96 Size: 2049 3928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2838 794 Size: 2044 3933 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2452 2944 2452 Size: 492 5485 BstYI r'GATC_y Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972 Size: 1335 367 385 17 1452 86 12 346 Cuts at: 5972 1972 Size: 1977 Fragments arranged by size: 1977 1452 1335 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2676 2676 Size: 5977 BtgI C'CryG_G Cuts at: 513 1065 2004 2996 3244 513 Size: 552 939 992 248 3246 Fragments arranged by size: 3246 992 939 552 248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2050 3082 487 Size: 683 56 824 1032 3382 Fragments arranged by size: 3382 1032 824 683 56 BtsI GCAGTG_nn' Cuts at: 2596 2791 3493 4287 4307 2596 Size: 195 702 794 20 4266 Fragments arranged by size: 4266 794 702 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942 Size: 54 1425 50 275 18 1594 889 1629 Cuts at: 5942 8 Size: 43 Fragments arranged by size: 1629 1594 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 1537 Size: 275 1612 4090 Fragments arranged by size: 4090 1612 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3086 3123 3907 1353 Size: 1733 37 784 3423 Fragments arranged by size: 3423 1733 784 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2529 2544 2896 3000 3032 4040 4637 Size: 393 897 15 352 104 32 1008 597 Cuts at: 4637 5424 5692 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1008 897 787 597 393 352 268 104 32 15 EcoNI CCTnn'n_nnAGG Cuts at: 3233 3233 Size: 5977 EcoO109I rG'GnC_Cy Cuts at: 3226 3226 Size: 5977 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2725 2976 5124 1678 Size: 1047 251 2148 2531 Fragments arranged by size: 2531 2148 1047 251 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 2382 2414 2918 Size: 32 135 32 712 32 66 32 504 Cuts at: 2918 2950 4618 4650 4692 4724 1373 Size: 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 712 504 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 3185 748 Size: 2437 3540 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 2246 5091 5275 1305 Size: 699 242 2845 184 2007 Fragments arranged by size: 2845 2007 699 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 3935 4294 4453 518 Size: 648 1264 1505 359 159 2042 Fragments arranged by size: 2042 1505 1264 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3089 3393 4070 5218 5463 1606 Size: 1483 304 677 1148 245 2120 Fragments arranged by size: 2120 1483 1148 677 304 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2996 513 Size: 552 939 992 3494 Fragments arranged by size: 3494 992 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3335 4880 1132 Size: 97 2106 1545 2229 Fragments arranged by size: 2229 2106 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 3225 3225 Size: 5977 PstI C_TGCA'G Cuts at: 830 2662 2834 2991 3329 830 Size: 1832 172 157 338 3478 Fragments arranged by size: 3478 1832 338 172 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 3089 3089 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SapI GCTCTTCn'nnn_ Cuts at: 3086 3086 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2034 2210 2658 2830 2987 3325 4460 Size: 219 989 176 448 172 157 338 1135 Cuts at: 4460 5141 5332 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 989 681 448 338 219 191 176 172 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4081 4982 5244 820 Size: 197 926 2138 901 262 1553 Fragments arranged by size: 2138 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2004 2996 3416 513 Size: 552 939 992 420 3074 Fragments arranged by size: 3074 992 939 552 420 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2989 3009 4004 4021 4174 4359 4804 Size: 1255 1409 20 995 17 153 185 445 Cuts at: 4804 325 Size: 1498 Fragments arranged by size: 1498 1409 1255 995 445 185 153 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 4223 5935 Size: 274 80 33 51 1443 168 2078 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 2078 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3242 3242 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 2058 4106 2058 Size: 2048 3929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI