(Circular) (Six-base) MAPSORT of: pFN21AE0045.seq Check: 4878 from: 1 to: 6688 pFN21AE0045 6688 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4498 278 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3624 4031 1937 Size: 1687 407 4594 Fragments arranged by size: 4594 1687 407 Acc65I G'GTAC_C Cuts at: 2784 4009 2784 Size: 1225 5463 AclI AA'CG_TT Cuts at: 1563 4815 5188 1563 Size: 3252 373 3063 Fragments arranged by size: 3252 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 3630 4751 6135 6403 1632 Size: 714 1284 1121 1384 268 1917 Fragments arranged by size: 1917 1384 1284 1121 714 268 AflII C'TTAA_G Cuts at: 820 1017 3979 820 Size: 197 2962 3529 Fragments arranged by size: 3529 2962 197 AflIII A'CryG_T Cuts at: 1203 2228 2459 5587 6564 1203 Size: 1025 231 3128 977 1327 Fragments arranged by size: 3128 1327 1025 977 231 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 5417 5417 Size: 6688 AleI CACnn'nnGTG Cuts at: 1166 3733 1166 Size: 2567 4121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2697 2729 2880 2912 2697 Size: 32 151 32 6473 Fragments arranged by size: 6473 151 32 32 AlwNI CAG_nnn'CTG Cuts at: 4431 6003 4431 Size: 1572 5116 ApaLI G'TGCA_C Cuts at: 3574 4745 5901 3574 Size: 1171 1156 4361 Fragments arranged by size: 4361 1171 1156 ApoI r'AATT_y Cuts at: 1493 2331 2856 3046 3997 4223 4375 6484 1493 Size: 838 525 190 951 226 152 2109 1697 Fragments arranged by size: 2109 1697 951 838 525 226 190 152 AseI AT'TA_AT Cuts at: 160 1032 2657 5242 160 Size: 872 1625 2585 1606 Fragments arranged by size: 2585 1625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 4013 1870 Size: 73 2070 4545 Fragments arranged by size: 4545 2070 73 BamHI G'GATC_C Cuts at: 3257 4018 4385 3257 Size: 761 367 5560 Fragments arranged by size: 5560 761 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2784 4009 5464 618 Size: 325 217 627 997 1225 1455 1842 Fragments arranged by size: 1842 1455 1225 997 627 325 217 BanII G_rGCy'C Cuts at: 1391 4007 1391 Size: 2616 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 2418 2582 928 Size: 971 316 203 164 5034 Fragments arranged by size: 5034 971 316 203 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4879 4913 1903 Size: 34 2942 34 3678 Fragments arranged by size: 3678 2942 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4580 5790 4580 Size: 1210 5478 BclI T'GATC_A Cuts at: 1548 3667 1548 Size: 2119 4569 BfrBI ATG'CAT Cuts at: 2985 3092 2985 Size: 107 6581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5299 136 Size: 107 122 71 631 605 3627 1525 Fragments arranged by size: 3627 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6683 6683 Size: 6688 BlpI GC'TnA_GC Cuts at: 4105 4105 Size: 6688 Bme1580I G_kGCm'C Cuts at: 1790 3578 4749 5905 1790 Size: 1788 1171 1156 2573 Fragments arranged by size: 2573 1788 1171 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4419 5377 442 Size: 416 53 83 399 24 3002 958 1753 Fragments arranged by size: 3002 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 2400 2586 5348 1239 Size: 996 165 186 2762 2579 Fragments arranged by size: 2762 2579 996 186 165 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4813 5678 5976 4813 Size: 865 298 5525 Fragments arranged by size: 5525 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 2616 5351 882 Size: 617 754 363 2735 2219 Fragments arranged by size: 2735 2219 754 617 363 BsaAI yAC'GTr Cuts at: 493 1204 2229 4479 493 Size: 711 1025 2250 2702 Fragments arranged by size: 2702 2250 1025 711 BsaBI GATnn'nnATC Cuts at: 3864 6682 3864 Size: 2818 3870 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4495 4877 5512 275 Size: 53 83 186 936 2962 382 635 1451 Fragments arranged by size: 2962 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5121 5519 5793 5940 6351 5121 Size: 398 274 147 411 5458 Fragments arranged by size: 5458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2697 2727 2880 2910 2959 Size: 30 165 30 1294 30 153 30 49 Cuts at: 2959 2989 3145 3175 3568 3598 1178 Size: 30 156 30 393 30 4268 Fragments arranged by size: 4268 1294 393 165 156 153 49 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2597 2820 3540 3573 3576 3870 1186 Size: 1411 223 720 33 3 294 4004 Fragments arranged by size: 4004 1411 720 294 223 33 3 BseYI C'CCAG_C Cuts at: 5891 6387 5891 Size: 496 6192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3060 3533 1163 Size: 573 1324 473 4318 Fragments arranged by size: 4318 1324 573 473 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4138 4899 5927 1540 Size: 275 186 2137 761 1028 2301 Fragments arranged by size: 2301 2137 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2645 3578 4749 4834 5905 2645 Size: 933 1171 85 1071 3428 Fragments arranged by size: 3428 1171 1071 933 85 BsmI GAATG_Cn' Cuts at: 3909 4199 4292 3909 Size: 290 93 6305 Fragments arranged by size: 6305 290 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2645 3578 4007 4749 4834 5905 1391 Size: 399 855 933 429 742 85 1071 2174 Fragments arranged by size: 2174 1071 933 855 742 429 399 85 BspEI T'CCGG_A Cuts at: 6351 6351 Size: 6688 BspHI T'CATG_A Cuts at: 2728 4577 6307 2728 Size: 1849 1730 3109 Fragments arranged by size: 3109 1849 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4043 844 Size: 3199 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4575 1394 Size: 429 2752 3507 Fragments arranged by size: 3507 2752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3286 3914 5183 5357 65 Size: 1183 2038 628 1269 174 1396 Fragments arranged by size: 2038 1396 1269 1183 628 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5332 5519 1700 Size: 110 3522 187 2869 Fragments arranged by size: 3522 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2231 96 Size: 2135 4553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6688 BssSI C'ACGA_G Cuts at: 4748 5760 4748 Size: 1012 5676 BstAPI GCAn_nnn'nTGC Cuts at: 794 3248 794 Size: 2454 4234 BstBI TT'CG_AA Cuts at: 5492 5492 Size: 6688 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6688 BstYI r'GATC_y Cuts at: 1972 2750 2925 3257 3686 3890 4018 4385 4770 Size: 778 175 332 429 204 128 367 385 Cuts at: 4770 4787 6239 6325 6337 6683 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 778 429 385 367 346 332 204 175 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2929 3115 513 Size: 552 1864 186 4086 Fragments arranged by size: 4086 1864 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5949 Fragments arranged by size: 5949 683 56 BtsI GCAGTG_nn' Cuts at: 3521 4204 4998 5018 3521 Size: 683 794 20 5191 Fragments arranged by size: 5191 794 683 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6688 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3804 3839 529 Size: 35 3240 35 3378 Fragments arranged by size: 3378 3240 35 35 DraI TTT'AAA Cuts at: 2131 3992 2131 Size: 1861 4827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5695 809 Size: 507 4379 1802 Fragments arranged by size: 4379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4135 5024 6653 Size: 54 1425 50 275 18 2305 889 1629 Cuts at: 6653 8 Size: 43 Fragments arranged by size: 2305 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4135 1537 Size: 275 2323 4090 Fragments arranged by size: 4090 2323 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 2211 2650 3649 4618 1353 Size: 708 150 439 999 969 3423 Fragments arranged by size: 3423 999 969 708 439 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5275 5649 5795 387 Size: 1064 138 3686 374 146 1280 Fragments arranged by size: 3686 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2346 2400 2586 3630 4751 5348 Size: 393 603 111 54 186 1044 1121 597 Cuts at: 5348 6135 6403 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1121 1044 787 603 597 393 268 186 111 54 EcoRI G'AATT_C Cuts at: 3997 4375 3997 Size: 378 6310 FspI TGC'GCA Cuts at: 5194 5194 Size: 6688 HaeII r_GCGC'y Cuts at: 1678 5835 1678 Size: 4157 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2408 2440 2598 2630 2959 Size: 32 135 32 836 32 158 32 329 Cuts at: 2959 2991 3145 3177 5329 5361 5403 5435 1373 Size: 32 154 32 2152 32 42 32 2626 Fragments arranged by size: 2626 2152 836 329 158 154 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4032 4278 669 Size: 988 281 2094 246 3079 Fragments arranged by size: 3079 2094 988 281 246 HindIII A'AGCT_T Cuts at: 748 2134 748 Size: 1386 5302 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KpnI G_GTAC'C Cuts at: 2788 4013 2788 Size: 1225 5463 MfeI C'AATT_G Cuts at: 4287 4287 Size: 6688 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5802 5986 1305 Size: 4497 184 2007 Fragments arranged by size: 4497 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4866 Fragments arranged by size: 4866 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2605 2623 3091 3404 3733 4646 5005 Size: 648 1439 18 468 313 329 913 359 Cuts at: 5005 5164 518 Size: 159 2042 Fragments arranged by size: 2042 1439 913 648 468 359 329 313 159 18 MspA1I CmG'CkG Cuts at: 1606 2564 3872 4104 4781 5929 6174 1606 Size: 958 1308 232 677 1148 245 2120 Fragments arranged by size: 2120 1308 1148 958 677 245 232 NcoI C'CATG_G Cuts at: 513 1065 2929 3115 513 Size: 552 1864 186 4086 Fragments arranged by size: 4086 1864 552 186 NdeI CA'TA_TG Cuts at: 387 3088 387 Size: 2701 3987 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 4135 4135 Size: 6688 NsiI A_TGCA'T Cuts at: 2987 3094 2987 Size: 107 6581 NspI r_CATG'y Cuts at: 1132 1229 2347 2463 3096 4046 5591 1132 Size: 97 1118 116 633 950 1545 2229 Fragments arranged by size: 2229 1545 1118 950 633 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6688 PciI A'CATG_T Cuts at: 2459 5587 2459 Size: 3128 3560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6688 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PmlI CAC'GTG Cuts at: 2229 2229 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 2729 2880 2912 4757 4789 6270 6302 2697 Size: 32 151 32 1845 32 1481 32 3083 Fragments arranged by size: 3083 1845 1481 151 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6688 PsiI TTA'TAA Cuts at: 4258 4258 Size: 6688 PstI C_TGCA'G Cuts at: 830 4040 830 Size: 3210 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 PvuII CAG'CTG Cuts at: 2564 3872 2564 Size: 1308 5380 SalI G'TCGA_C Cuts at: 1936 4030 1936 Size: 2094 4594 SapI GCTCTTCn'nnn_ Cuts at: 2211 2650 3649 2211 Size: 439 999 5250 Fragments arranged by size: 5250 999 439 SbfI CC_TGCA'GG Cuts at: 4040 4040 Size: 6688 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SfcI C'TryA_G Cuts at: 826 1045 2440 4036 5171 5852 6043 826 Size: 219 1395 1596 1135 681 191 1471 Fragments arranged by size: 1596 1471 1395 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 4015 1872 Size: 2143 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3979 4792 5693 5955 820 Size: 197 926 2036 813 901 262 1553 Fragments arranged by size: 2036 1553 926 901 813 262 197 SnaBI TAC'GTA Cuts at: 493 4479 493 Size: 3986 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 2117 3287 4612 5 Size: 47 2065 1170 1325 2081 Fragments arranged by size: 2081 2065 1325 1170 47 StuI AGG'CCT Cuts at: 1517 3698 1517 Size: 2181 4507 StyI C'CwwG_G Cuts at: 513 1065 2258 2929 3115 4127 513 Size: 552 1193 671 186 1012 3074 Fragments arranged by size: 3074 1193 1012 671 552 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4715 4732 4885 5070 5515 325 Size: 1255 3135 17 153 185 445 1498 Fragments arranged by size: 3135 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2231 2530 3365 Size: 274 80 33 51 1443 254 299 835 Cuts at: 3365 3548 4934 6646 96 Size: 183 1386 1712 138 Fragments arranged by size: 1712 1443 1386 835 299 274 254 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3249 3281 3568 3600 6648 6680 3249 Size: 32 287 32 3048 32 3257 Fragments arranged by size: 3257 3048 287 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6688 XbaI T'CTAG_A Cuts at: 4024 4024 Size: 6688 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6688 XmaI C'CCGG_G Cuts at: 1870 4013 1870 Size: 2143 4545 XmnI GAAnn'nnTTC Cuts at: 2400 2491 2510 2895 4817 2400 Size: 91 19 385 1922 4271 Fragments arranged by size: 4271 1922 385 91 19 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4496 276 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI