Restriction map for
pFN21AB0989
(Linear) (Six-base) MAPSORT of: KIBB0989.seq Check: 1252 from: 1 to: 405
KIBB0989.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
ApaI G_GGCC'C
Cuts at: 0 47 237 405
Size: 47 190 168
Fragments arranged by size:
190 168 47
AvaI C'yCGr_G
Cuts at: 0 58 230 237 405
Size: 58 172 7 168
Fragments arranged by size:
172 168 58 7
BanI G'GyrC_C
Cuts at: 0 18 405
Size: 18 387
BanII G_rGCy'C
Cuts at: 0 47 65 215 237 405
Size: 47 18 150 22 168
Fragments arranged by size:
168 150 47 22 18
BlpI GC'TnA_GC
Cuts at: 0 95 382 405
Size: 95 287 23
Fragments arranged by size:
287 95 23
Bme1580I G_kGCm'C
Cuts at: 0 47 237 405
Size: 47 190 168
Fragments arranged by size:
190 168 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 405
Size: 229 176
Bpu10I CC'TnA_GC
Cuts at: 0 39 405
Size: 39 366
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 405
Size: 202 30 173
Fragments arranged by size:
202 173 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 405
Size: 72 333
BseYI C'CCAG_C
Cuts at: 0 116 405
Size: 116 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 405
Size: 272 133
BsiHKAI G_wGCw'C
Cuts at: 0 65 383 405
Size: 65 318 22
Fragments arranged by size:
318 65 22
Bsp1286I G_dGCh'C
Cuts at: 0 47 65 215 237 383 405
Size: 47 18 150 22 146 22
Fragments arranged by size:
150 146 47 22 22 18
BsrBI CCG'CTC
Cuts at: 0 94 405
Size: 94 311
BstYI r'GATC_y
Cuts at: 0 338 405
Size: 338 67
EarI CTCTTCn'nnn_
Cuts at: 0 193 405
Size: 193 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 207 331 405
Size: 207 124 74
Fragments arranged by size:
207 124 74
EcoICRI GAG'CTC
Cuts at: 0 63 405
Size: 63 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 405
Size: 229 176
EcoO109I rG'GnC_Cy
Cuts at: 0 43 44 51 234 405
Size: 43 1 7 183 171
Fragments arranged by size:
183 171 43 7 1
FspI TGC'GCA
Cuts at: 0 355 405
Size: 355 50
HaeII r_GCGC'y
Cuts at: 0 206 405
Size: 206 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 307 339 405
Size: 202 32 73 32 66
Fragments arranged by size:
202 73 66 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 405
Size: 90 315
MspA1I CmG'CkG
Cuts at: 0 15 73 99 312 405
Size: 15 58 26 213 93
Fragments arranged by size:
213 93 58 26 15
NspI r_CATG'y
Cuts at: 0 146 405
Size: 146 259
PasI CC'CwG_GG
Cuts at: 0 47 104 405
Size: 47 57 301
Fragments arranged by size:
301 57 47
PspOMI G'GGCC_C
Cuts at: 0 43 233 405
Size: 43 190 172
Fragments arranged by size:
190 172 43
PvuII CAG'CTG
Cuts at: 0 15 99 405
Size: 15 84 306
Fragments arranged by size:
306 84 15
SacI G_AGCT'C
Cuts at: 0 65 405
Size: 65 340
SapI GCTCTTCn'nnn_
Cuts at: 0 193 405
Size: 193 212
SmaI CCC'GGG
Cuts at: 0 232 239 405
Size: 232 7 166
Fragments arranged by size:
232 166 7
TatI w'GTAC_w
Cuts at: 0 299 405
Size: 299 106
XmaI C'CCGG_G
Cuts at: 0 230 237 405
Size: 230 7 168
Fragments arranged by size:
230 168 7
Enzymes that do cut:
ApaI AvaI BanI BanII BlpI Bme1580I BpmI Bpu10I
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BstYI
EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
MmeI MspA1I NspI PasI PspOMI PvuII SacI SapI
SmaI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI