Restriction map for
pFN21AB9904
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BfaI BfrI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoRI EcoT22I
FseI FspI HaeII HindIII HpaI
KasI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 968 GGTACC G^GTACC
AccI 1294 GTAGAC GT^[AC][GT]AC
AccII 5 CGCG CG^CG
AcyI 35 GGCGTC G[GA]^CG[CT]C
AfaI 969 GTAC GT^AC
AlwI 1167 GGATC GGATC(4/5)
AlwNI 1339 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 968 GGTACC G^GTACC
AspHI 1147 GTGCTC G[AT]GC[AT]^C
AspI 1189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 840 GGATAC GTATCC(6/5)
BclI 834 TGATCA T^GATCA
BlpI 1753 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1753 GCTCAGC GC^T[ACGT]AGC
BsaHI 35 GGCGTC G[GA]^CG[CT]C
BsgI 1507 CTGCAC GTGCAG(16/14)
BsiHKAI 1147 GTGCTC G[AT]GC[AT]^C
BspHI 1241 TCATGA T^CATGA
BsrFI 196 GCCGGC [GA]^CCGG[CT]
BstUI 5 CGCG CG^CG
CelII 1753 GCTCAGC GC^T[ACGT]AGC
CfoI 1620 GCGC GCG^C
Cfr10I 196 GCCGGC [GA]^CCGG[CT]
EagI 162 CGGCCG C^GGCCG
EclXI 162 CGGCCG C^GGCCG
Eco52I 162 CGGCCG C^GGCCG
EcoNI 351 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 763 GATATC GAT^ATC
FbaI 834 TGATCA T^GATCA
HgaI 36 GCGTC GACGC(5/10)
HhaI 1620 GCGC GCG^C
Hin1I 35 GGCGTC G[GA]^CG[CT]C
HinP1I 1620 GCGC G^CGC
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
KpnI 968 GGTACC GGTAC^C
MaeII 1502 ACGT A^CGT
MslI 979 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 5 CGCG CG^CG
NaeI 196 GCCGGC GCC^GGC
NcoI 1265 CCATGG C^CATGG
NgoMIV 196 GCCGGC G^CCGGC
PflFI 1189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1241 TCATGA T^CATGA
RsaI 969 GTAC GT^AC
SfcI 1292 CTGTAG C^T[GA][CT]AG
SgrAI 195 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 20 CCCGGG CCC^GGG
SmlI 181 CTCAAG C^T[CT][GA]AG
Tth111I 1189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 20 CCCGGG C^CCGGG
XmaI 20 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 709 GAATTT [GA]^AATT[CT]
1237 AAATTC
ApoI 709 GAATTT [GA]^AATT[CT]
1237 AAATTC
Asp700I 819 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1596 GAAAGTATTC
AvaI 20 CCCGGG C^[CT]CG[GA]G
1117 CCCGAG
BanI 968 GGTACC G^G[CT][GA]CC
1091 GGCACC
BglI 238 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
730 GCCCAGCGGGC
BglII 533 AGATCT A^GATCT
1556 AGATCT
BsiEI 82 CGGTCG CG[GA][CT]^CG
162 CGGCCG
BsmFI 548 GGGAC GGGAC(10/14)
848 GGGAC
BsoBI 20 CCCGGG C^[CT]CG[GA]G
1117 CCCGAG
BsrDI 489 CATTGC GCAATG(2/0)
1220 GCAATG
BstYI 533 AGATCT [GA]^GATC[CT]
1556 AGATCT
EcoT14I 740 CCAAGG C^C[AT][AT]GG
1265 CCATGG
MflI 533 AGATCT [GA]^GATC[CT]
1556 AGATCT
SfaNI 1660 GATGC GCATC(5/9)
1484 GCATC
StuI 359 AGGCCT AGG^CCT
743 AGGCCT
StyI 740 CCAAGG C^C[AT][AT]GG
1265 CCATGG
TaqI 613 TCGA T^CGA
794 TCGA
TfiI 1435 GAATC G^A[AT]TC
1648 GAATC
TthHB8I 613 TCGA T^CGA
794 TCGA
XcmI 380 CCATCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1265 CCATGGTGGAGATGG
XhoII 533 AGATCT [GA]^GATC[CT]
1556 AGATCT
XmnI 819 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1596 GAAAGTATTC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI ApaI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoO65I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TseI Tsp45I
Tsp509I TspRI