(Circular) (Six-base) MAPSORT of: pFN21AB9904.seq Check: 6264 from: 1 to: 6472 pFN21AB9904 6472 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4282 278 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3300 3815 1937 Size: 1363 515 4594 Fragments arranged by size: 4594 1363 515 Acc65I G'GTAC_C Cuts at: 2973 3793 2973 Size: 820 5652 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 3418 3758 4535 5919 6187 1632 Size: 1181 605 340 777 1384 268 1917 Fragments arranged by size: 1917 1384 1181 777 605 340 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3527 1184 Size: 2343 4129 AlwNI CAG_nnn'CTG Cuts at: 3349 4215 5787 3349 Size: 866 1572 4034 Fragments arranged by size: 4034 1572 866 ApaI G_GGCC'C Cuts at: 2176 2964 3009 3071 2176 Size: 788 45 62 5577 Fragments arranged by size: 5577 788 62 45 ApaLI G'TGCA_C Cuts at: 4529 5685 4529 Size: 1156 5316 ApoI r'AATT_y Cuts at: 1493 2714 3242 3781 4007 4159 6268 1493 Size: 1221 528 539 226 152 2109 1697 Fragments arranged by size: 2109 1697 1221 539 528 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2025 3122 3797 1870 Size: 73 82 1097 675 4545 Fragments arranged by size: 4545 1097 675 82 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2973 3096 3793 5248 618 Size: 325 217 627 1186 123 697 1455 1842 Fragments arranged by size: 1842 1455 1186 697 627 325 217 123 BanII G_rGCy'C Cuts at: 1391 2176 2964 3009 3071 3791 1391 Size: 785 788 45 62 720 4072 Fragments arranged by size: 4072 788 785 720 62 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3193 3202 3414 928 Size: 971 1294 9 212 3986 Fragments arranged by size: 3986 1294 971 212 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2839 4364 5574 2839 Size: 1525 1210 3737 Fragments arranged by size: 3737 1525 1210 BclI T'GATC_A Cuts at: 1548 2839 1548 Size: 1291 5181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2249 2741 5083 Size: 107 122 71 631 605 577 492 2342 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 2342 1525 631 605 577 492 122 107 71 BglII A'GATC_T Cuts at: 2538 3561 6467 2538 Size: 1023 2906 2543 Fragments arranged by size: 2906 2543 1023 BlpI GC'TnA_GC Cuts at: 3759 3889 3759 Size: 130 6342 Bme1580I G_kGCm'C Cuts at: 1790 2176 2737 2964 3009 3071 3099 4533 5689 Size: 386 561 227 45 62 28 1434 1156 Cuts at: 5689 1790 Size: 2573 Fragments arranged by size: 2573 1434 1156 561 386 227 62 45 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2303 3082 4203 Size: 416 53 83 399 24 886 779 1121 Cuts at: 4203 5161 442 Size: 958 1753 Fragments arranged by size: 1753 1121 958 886 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3646 3646 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 3554 3665 3692 5132 1239 Size: 833 1482 111 27 1440 2579 Fragments arranged by size: 2579 1482 1440 833 111 27 Bpu10I CC'TnA_GC Cuts at: 2178 2178 Size: 6472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 4597 5462 5760 2171 Size: 2426 865 298 2883 Fragments arranged by size: 2883 2426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5135 882 Size: 617 3636 2219 Fragments arranged by size: 3636 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 4279 4661 5296 Size: 53 83 186 936 508 2238 382 635 Cuts at: 5296 275 Size: 1451 Fragments arranged by size: 2238 1451 936 635 508 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 1178 Size: 30 165 30 682 30 5535 Fragments arranged by size: 5535 682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 3560 3622 1186 Size: 1067 1307 62 4036 Fragments arranged by size: 4036 1307 1067 62 BseYI C'CCAG_C Cuts at: 2506 2736 3500 3574 5675 6171 2506 Size: 230 764 74 2101 496 2807 Fragments arranged by size: 2807 2101 764 496 230 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3497 1163 Size: 573 1761 4138 Fragments arranged by size: 4138 1761 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2090 2170 3922 4683 5711 1540 Size: 275 186 89 80 1752 761 1028 2301 Fragments arranged by size: 2301 1752 1028 761 275 186 89 80 BsiHKAI G_wGCw'C Cuts at: 3156 4533 4618 5689 3156 Size: 1377 85 1071 3939 Fragments arranged by size: 3939 1377 1071 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2737 2964 3009 3071 3099 3156 Size: 399 386 561 227 45 62 28 57 Cuts at: 3156 3791 4533 4618 5689 1391 Size: 635 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 635 561 399 386 227 85 62 57 45 28 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 3246 4361 6091 3246 Size: 1115 1730 3627 Fragments arranged by size: 3627 1730 1115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3827 844 Size: 2983 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4359 1394 Size: 429 2536 3507 Fragments arranged by size: 3507 2536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 3232 4967 5141 65 Size: 1183 1245 739 1735 174 1396 Fragments arranged by size: 1735 1396 1245 1183 739 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2201 5116 5303 1700 Size: 110 391 2915 187 2869 Fragments arranged by size: 2915 2869 391 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 4532 5544 4532 Size: 1012 5460 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6472 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 2501 2885 3056 3538 1184 Size: 1317 384 171 482 4118 Fragments arranged by size: 4118 1317 482 384 171 BstYI r'GATC_y Cuts at: 1972 2538 3561 3802 4169 4554 4571 6023 6109 Size: 566 1023 241 367 385 17 1452 86 Cuts at: 6109 6121 6467 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1023 566 385 367 346 241 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2625 2965 3270 513 Size: 552 1560 340 305 3715 Fragments arranged by size: 3715 1560 552 340 305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2111 2720 3582 3678 487 Size: 683 56 885 609 862 96 3281 Fragments arranged by size: 3281 885 862 683 609 96 56 BtsI GCAGTG_nn' Cuts at: 2164 2469 3753 3988 4782 4802 2164 Size: 305 1284 235 794 20 3834 Fragments arranged by size: 3834 1284 794 305 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2857 2892 529 Size: 35 2293 35 4109 Fragments arranged by size: 4109 2293 35 35 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5479 809 Size: 507 4163 1802 Fragments arranged by size: 4163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2167 3587 Size: 54 1425 50 275 18 307 30 1420 Cuts at: 3587 3919 4808 6437 8 Size: 332 889 1629 43 Fragments arranged by size: 1629 1425 1420 889 332 307 275 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2167 3919 1537 Size: 275 355 1752 4090 Fragments arranged by size: 4090 1752 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4402 1353 Size: 3049 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 2139 5059 5433 5579 387 Size: 1064 138 456 94 2920 374 146 1280 Fragments arranged by size: 2920 1280 1064 456 374 146 138 94 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2813 3418 3554 3665 3692 3758 Size: 393 440 741 605 136 111 27 66 Cuts at: 3758 4535 5132 5919 6187 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 741 605 597 440 393 268 136 111 66 27 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 6472 EcoO109I rG'GnC_Cy Cuts at: 2173 2326 2960 2961 3006 3067 2173 Size: 153 634 1 45 61 5578 Fragments arranged by size: 5578 634 153 61 45 1 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 EcoRV GAT'ATC Cuts at: 2770 2770 Size: 6472 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2372 2372 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 5619 1678 Size: 3941 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3638 3670 5113 5145 5187 Size: 32 135 32 2066 32 1443 32 42 Cuts at: 5187 5219 1373 Size: 32 2626 Fragments arranged by size: 2626 2066 1443 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2518 3816 4062 669 Size: 988 281 580 1298 246 3079 Fragments arranged by size: 3079 1298 988 580 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KpnI G_GTAC'C Cuts at: 2977 3797 2977 Size: 820 5652 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3384 5586 5770 1305 Size: 2079 2202 184 2007 Fragments arranged by size: 2202 2079 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4650 Fragments arranged by size: 4650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2988 4430 4789 4948 518 Size: 648 1822 1442 359 159 2042 Fragments arranged by size: 2042 1822 1442 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2740 3532 3888 4565 5713 5958 1606 Size: 474 660 792 356 677 1148 245 2120 Fragments arranged by size: 2120 1148 792 677 660 474 356 245 NaeI GCC'GGC Cuts at: 2203 2203 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 3270 513 Size: 552 2205 3715 Fragments arranged by size: 3715 2205 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NgoMIV G'CCGG_C Cuts at: 2201 2201 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 3830 5375 1132 Size: 97 2601 1545 2229 Fragments arranged by size: 2601 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2549 1348 Size: 1201 5271 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6472 PfoI T'CCnGG_A Cuts at: 2567 3443 2567 Size: 876 5596 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4541 4573 6054 6086 4541 Size: 32 1481 32 4927 Fragments arranged by size: 4927 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 2172 2960 3005 3067 2172 Size: 788 45 62 5577 Fragments arranged by size: 5577 788 62 45 PstI C_TGCA'G Cuts at: 830 3824 830 Size: 2994 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 3297 3820 4955 5636 5827 826 Size: 219 2252 523 1135 681 191 1471 Fragments arranged by size: 2252 1471 1135 681 523 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 2201 1700 Size: 501 5971 SmaI CCC'GGG Cuts at: 1872 2027 3799 1872 Size: 155 1772 4545 Fragments arranged by size: 4545 1772 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4576 5477 5739 820 Size: 197 926 243 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 2366 2750 1517 Size: 849 384 5239 Fragments arranged by size: 5239 849 384 StyI C'CwwG_G Cuts at: 513 1065 2745 3270 3911 513 Size: 552 1680 525 641 3074 Fragments arranged by size: 3074 1680 641 552 525 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2520 3210 3557 4499 4516 4669 4854 Size: 1255 940 690 347 942 17 153 185 Cuts at: 4854 5299 325 Size: 445 1498 Fragments arranged by size: 1498 1255 942 940 690 445 347 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4718 6430 96 Size: 274 80 33 51 1443 2741 1712 138 Fragments arranged by size: 2741 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 6432 6464 2085 Size: 32 4315 32 2093 Fragments arranged by size: 4315 2093 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3197 1316 Size: 1881 4591 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2392 3277 2392 Size: 885 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 2025 3797 1870 Size: 155 1772 4545 Fragments arranged by size: 4545 1772 155 XmnI GAAnn'nnTTC Cuts at: 2828 3605 4601 2828 Size: 777 996 4699 Fragments arranged by size: 4699 996 777 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4280 276 Size: 53 83 186 936 2746 2468 Fragments arranged by size: 2746 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI